Application of Human Papillomavirus in Screening for Cervical Cancer and Precancerous Lesions

Wang, Jin-Liang,Yang, Yi-Zhuo,Dong, Wei-Wei,Sun, Jing,Tao, Hai-Tao,Li, Rui-Xin,Hu, Yi Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Cervical cancer is a commonly-encountered malignant tumor in women. Cervical screening is particularly important due to early symptoms being deficient in specificity. The main purpose of the study is to assess the application value of cervical thinprep cytologic test (TCT) and human papillomavirus (HPV) detection in screening for cervical cancer and precancerous lesions. In the study, cervical TCT and HPV detection were simultaneously performed on 12,500 patients selected in a gynecological clinic. Three hundred patients with positive results demonstrated by cervical TCT and/or HPV detection underwent cervical tissue biopsy under colposcopy, and pathological results were considered as the gold standard. The results revealed that 200 out of 12,500 patients were abnormal by TCT, in which 30 cases pertained to equivocal atypical squamous cells (ASCUS), 80 cases to low squamous intraepithelial lesion (LSIL), 70 cases to high squamous intraepithelial lesion (HSIL) and 20 cases to squamous cell carcinoma (SCC). With increasing pathological grade of cervical biopsy, however, TCT positive rates did not rise. Two hundred and eighty out of 12,500 patients were detected as positive for HPV infection, in which 50 cases were chronic cervicitis and squamous metaplasia, 70 cases cervical intraepithelial neoplasia (CIN) I, 60 cases CIN II, 70 cases CIN III and 30 cases invasive cervical carcinoma. Two hundred and thirty patients with high-risk HPV infection were detected. With increase in pathological grade, the positive rate of high-risk HPV also rose. The detection rates of HPV detection to CIN III and invasive cervical carcinoma as well as the total detection rate of lesions were significantly higher than that of TCT. Hence, HPV detection is a better method for screening of cervical cancer at present.

Rethinking the South China Sea Issue: A Perspective of Sino-U.S. Relations

( Yi Wei Wang ) 인하대학교 국제관계연구소 2006 Pacific Focus Vol.21 No.1

Three essential factors of international politics underlie current controversies regarding the South China Sea: sovereignty, resources and strategy. Before the 1980`s, the United States maintained a completely "neutral position" towards this issue for a number of historical reasons. However, when the 1982 UNCLOS Ⅲ entered into force in 1994, the South China Sea joined Taiwan and the tensions on the Korean Peninsula as one of three potential flashpoints in the Asia-Pacific region. Since 1994, American foreign policy regarding the South China Sea underwent three stages of development: prevention-deterrence-practice. This article analyzes these changes by focusing on the development of America`s Asia-Pacific security strategy, especially one of its main outcomes: the development of the Sino-U.S. relationship. From balance of power and geopolitical theories, in the long run, the South China Sea issue may become a global fulcrum between the U.S. and China, which probably will become a global power in the future. Even as a regional power today, it is well known that the Taiwan issue has led to conflict and competition with the U.S. for more than half a century. To avoid falling into the same old trap again as with the Taiwan issue in the past, we must prepare for a rainy day in solving the South China Sea issue, in essence, to find a balance between China`s position, which represents a newcomer`s demands, and international maritime law, which represents the vested interests of the international system. We need a long-term perspective to deal with the Sino-U.S relationship.

Notch1 promotes the pericytemyofibroblast transition in idiopathic pulmonary fibrosis through the PDGFR/ ROCK1 signal pathway

Yi-Chun Wang,Qiong Chen,Jun-Ming Luo,Jing Nie,Qing-He Meng,Wei Shuai,Han Xie,Jia-Mei Xia,Hui Wang 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The goals of this study were to investigate the role of the Notch1/PDGFRβ/ROCK1 signaling pathway in the pathogenesis of pulmonary fibrosis and to explore the possibility of treating fibrosis by targeting Notch1. Lung tissues from patients with pulmonary fibrosis were examined for the expression of Notch1/PDGFRβ/ROCK1 using RT-qPCR, western blotting, and immunostaining. Cultured mouse lung pericytes were transfected with Notch1-overexpressed vectors or shRNA targeting PDGFRβ/ROCK1 to examine cell behaviors, including proliferation, cell cycle arrest, and differentiation toward myofibroblasts. Finally, a mouse pulmonary fibrosis model was prepared, and a Notch1 inhibitor was administered to observe tissue morphology and pericyte cell behaviors. Human pulmonary fibrotic tissues presented with overexpression of Notch1, PDGFRβ, and ROCK1, in addition to a prominent transition of pericytes into myofibroblasts. In cultured mouse lung pericytes, overexpression of Notch1 led to the accelerated proliferation and differentiation of cells, and it also increased the expression of the PDGFRβ and ROCK1 proteins. The knockdown of PDGFRβ/ROCK1 in pericytes remarkably suppressed pericyte proliferation and differentiation. As further substantiation, the administration of a Notch1 inhibitor in a mouse model of lung fibrosis inhibited the PDGFRβ/ROCK1 pathway, suppressed pericyte proliferation and differentiation, and alleviated the severity of fibrosis. Our results showed that the Notch1 signaling pathway was aberrantly activated in pulmonary fibrosis, and this pathway may facilitate disease progression via mediating pericyte proliferation and differentiation. The inhibition of the Notch1 pathway may provide one promising treatment strategy for pulmonary fibrosis.

Influence of Ga(Al)As substrates on surface morphology and critical thickness of InGaAs quantum dots

Yi Wang,Xiang Guo,Jiemin Wei,Chen Yang,Zijiang Luo,Jihong Wang,Zhao Ding 한국물리학회 2019 Current Applied Physics Vol.19 No.4

Influence of Ga(Al)As substrates on surface morphology of InGaAs quantum dots and critical thickness of In0.5Ga0.5As film grown by molecular beam epitaxy is investigated. The In0.5Ga0.5As quantum dots are grown on (001) surfaces of GaAs and Al0.25Ga0.75 A at 450 °C, scanning tunneling microscope images show that the size of quantum dots varied slightly for 10 ML of In0.5Ga0.5As grown on GaAs and Al0.25Ga0.75As surfaces. Reflection high energy electron diffraction (RHEED) is used to monitor the growth of 4 monolayers (ML) In0.5Ga0.5As on Al0.25Ga0.75As and GaAs surfaces during deposition. The critical thickness is theoretically calculated by adding energy caused by surface roughness and heat from substrate. The calculations show that the critical thickness of In0.5Ga0.5As grown on GaAs and Al0.25Ga0.75As are 3.2 ML and 3.8 ML, respectively. The theoretical calculation agrees with the experimental results.

Genetic structure of six cattle populations revealed by transcriptomewide SNPs and gene expression

Wei Wang,Huai Wang,Hui Tang,Jia Gan,Changgeng Shi,Qing Lu,Donghui Fang,Jun Yi,Maozhong Fu 한국유전학회 2018 Genes & Genomics Vol.40 No.7

There are abundant cattle breeds/populations in China, and the systematic discovery of genomic variants is essential for performing the marker assisted selection and conservation of genetic resources. In the present study, we employed whole transcriptome sequencing (RNA-Seq) technology for revealing genetic structure among six Chinese cattle populations according to transcriptome-wide SNPs and gene expression. A total of 68,094 variants consisting of 61,754 SNPs and 6340 InDels were detected and widely distributed among all chromosomes, by which the clear patterns of population structures were revealed. We also found the significantly differential density of variant distribution among genes. Additionally, we totally assembled 15,992 genes and detected obvious differences on the expression profiles among populations. In contrast to genomic variants, the measure of gene expression levels failed to support the expected population structure. Here, we provided a global landscape on the differential expression genes among these cattle populations.

China`s Role in Dealing with the North Korean Nuclear Issue

Yi Wei Wang 한국학술연구원 2005 Korea Observer Vol.36 No.3

Lentivirus-mediated shRNA Interference Targeting SLUG Inhibits Lung Cancer Growth and Metastasis

Wang, Yao-Peng,Wang, Ming-Zhao,Luo, Yi-Ren,Shen, Yi,Wei, Zhao-Xia Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Objective: Lung cancer is a deadly cancer, whose kills more people worldwide than any other malignancy. SLUG (SNAI2, Snail2) is involved in the epithelial mesenchymal transition in physiological and in pathological contexts and is implicated in the development and progression of lung cancer. Methods: We constructed a lentivirus vector with SLUG shRNA (LV-shSLUG). LV-shSLUG and a control lentivirus were infected into the non-small cell lung cancer cell A549 and real-time PCR, Western blot and IHC were applied to assess expression of the SLUG gene. Cell proliferation and migration were detected using MTT and clony formation methods. Results: Real-time PCR, Western Blot and IHC results confirmed down-regulation of SLUG expression by its shRNA by about 80%~90% at both the mRNA and protein levels. Knockdown of SLUG significantly suppressed lung cancer cell proliferation. Furthermore, knockdown of SLUG significantly inhibited lung cancer cell invasion and metastasis. Finally, knockdown of SLUG induced the down-regulation of Bcl-2 and up-regulation of E-cadherin. Conclusion: These results indicate that SLUG is a newly identified gene associated with lung cancer growth and metastasis. SLUG may serve as a new therapeutic target for the treatment of lung cancer in the future.

TC1 (C8orf4) is involved in ERK1/2 pathway-regulated G1-to S-phase transition

( Yi Dong Wang ),( Guo Hui Bian ),( Xiao Yan Lv ),( Rong Zheng ),( Huan Sun ),( Zheng Zhang ),( Ye Chen ),( Qin Wei Li ),( Yan Xiao ),( Qiu Tan Yang ),( Jian Zhong Ai ),( Yu Quan Wei ),( Qin Zhou ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.10

Evolutionary and functional implications of 3′ untranslated region length of mRNAs by comprehensive investigation among four taxonomically diverse metazoan species

Wei Wang,Dong‑hui Fang,Jia Gan,Yi Shi,Hui Tang,Huai Wang,Mao‑zhong Fu,Jun Yi 한국유전학회 2019 Genes & Genomics Vol.41 No.7

Background In eukaryotic organisms, it has been well acknowledged that 3′ untranslated regions (3′ UTRs) of mRNA are actively involved in post-transcriptional regulations of gene expression. Although both shortening and lengthening of 3′ UTRs of specific candidate genes were explicitly documented to have functional consequences, landscape of 3′ UTR lengths in relation to evolutionary dynamics and biological meanings remains to be elucidated when large-scale data become available. Objectives The primary objective of this study was to revealed different inter- and intra-species patterns on length distribution of 3′ UTRs in comparison with 5′ UTRs and coding regions. Methods In the present study, we investigated 3′ UTR lengths in a highly curated set of 57,135 mRNA sequences among four well-studied and taxonomically diverse metazoan species (fruit fly, zebrafish, mouse and human). Results The average length ratio of 3′–5′ UTRs considerably increased from fruit fly (twofold) to human (fivefold). Moreover, genes can be characterized by the obviously different patterns of evolutionary change on 3′ UTR lengths. By utilizing the Gene Ontology annotations, genes with differential lengths of 3′ UTRs were suggested to have the divergent functional implications. In human, we further revealed that ubiquitously transcribed genes had higher median lengths of 3′ UTRs than the genes of tissue-restricted expressions. Conclusion We conducted a comprehensive analysis and provided an overview regarding 3′ UTRs biology of mRNAs in animals, whereas the mechanistic explanations of 3′ UTRs length variation in correlation to regulation of gene expression still remain to be further studied.

Enhanced low-temperature activity and huimid-SO2 resistance of MnFe-based multi-oxide catalysts for the marine NH3-SCR reaction

Yi Wei,Wenhua Li,Qi Wang,Mengyu Liu,Peiyuan Liang,Lina Wang,Tianjun Sun 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

Several MnFe-based multi-metal oxides were synthesized as NH3-SCR catalysts by a simple coprecipitationmethod for abating NOx of marine diesel exhausts. The Co and Nb-doped MnFeCeAl catalysts exhibitNOx conversion over 90% and N2 selectivity above 95% at 180–270 C, especially the MnFeCeAlCo catalystscan inhibit nearly all sulfate species growth within 150 ppm humid-SO2 gases at 225 C. The structuralcharacterization results revealed that Co, Nb, Sm, and Sb doping can enhance interactions amongdifferent components and promote active component dispersion. Temperature programmed analysisindicated that the Co doping is not only more favorable for improving redox properties, but can alsoenhance the surface acidity, which are advantageous to improve the activity, N2 selectivity, andhumid-SO2 resistance. Moreover, the XPS results implied that the binding energy shift or the valence variationof the Sm, Sb, Nb, and Co species on catalyst surfaces are favored to raise the atomic ratios of highvalentMn species and surface adsorbed oxygen, which can promote the redox property significantly andfurther facilitate SCR activity. Accordingly, the excellent activity and humid-SO2 tolerance of theMnFeCeAlCo catalyst should attribute to its lower redox temperature, strong interaction between oxides,47.3% surface Mn4+/Mn3+ species, and 71.8% adsorbed oxygen, which provide a method for improving theSCR performances of MnFe-based catalysts with humid SO2 resistance.