STATE LEGITIMACY, STATE POLICY, AND THE DEVELOPMENT OF THE 1989 BEIJING STUDENT MOVEMENT

Zhao, Ding Xin 경남대학교 극동문제연구소 1999 ASIAN PERSPECTIVE Vol.23 No.2

Transcriptional profiling analysis of OsDT11-mediated ABA-dependent signal pathway for drought tolerance in rice

Zhao Man,Ju Yanhu,Zhao Bo,Li Xiaoming,Dai LIli,Qu Jicheng,Chu Zhaohui,Ding Xinhua 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5

Small cysteine-rich peptides (CRPs) are important in plant responses to biotic and abiotic stresses. OsDT11, encoding an 88 amino acid CRP-type protein, has been reported to enhance tolerance to drought in rice (Oryza sativa L.) without negatively afecting other agronomic traits. However, the molecular mechanisms of OsDT11-mediated drought tolerance are still unclear. Here, we performed RNA-Seq analysis to compare the transcriptome profles between wild-type (WT) and OsDT11-overexpressing (OE) rice lines under drought stress or under control (non-drought) conditions. A total of 1570 and 1421 diferentially expressed genes were identifed in the OE lines and the WT under drought treatment, respectively, compared to non-drought conditions. Gene ontology analysis indicated that the 430 up-regulated genes in common to both OE and the WT lines were induced for functions related to responses to water deprivation and to abscisic acid (ABA). More than half of these genes had higher expression in the OE than in the WT under drought stress. In the OE, but not in the WT, 294 genes were specifcally up-regulated under drought stress and were functionally enriched in starch and sucrose biosyn�thetic processes and in response to stress. This implies that OsDT11 not only triggers strongly response to drought stress, but also alters several metabolic processes to enhance drought tolerance. Gene expression profling suggests that OsDT11 confers drought tolerance by mediating an enhanced response to drought stress in an ABA-dependent signaling pathways.

Path Smoothing for Five-Axis Machine Tools Using Dual Quaternion Approximation with Dominant Points

Xin Zhao,Huan Zhao,Xiangfei Li,Han Ding 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.5

Nowadays, G01 blocks are still the most widely utilized representation form for the tool paths in the five-axis machine tools. Tangency and curvature discontinuities of the linear tool paths may lead to frequent feedrate fluctuation and acceleration variation, which inevitably deteriorate the machining efficiency and quality. To solve this problem, this paper proposed a dual quaternion B-spline approximation method of dominant points to generate smooth tool paths for five-axis CNC machine tools. First, the dominant points, which can characterize the shape of the linear path, are selected for the tool tip location and the tool orientation simultaneously. Such scheme can significantly compress the number of the control points and release the computational load. Then, the linear tool paths are converted to dual quaternions and approximated to dual quaternion B-spline, which can realize the parameter synchronization for the tool tip and tool orientation, and avoid the fitting oscillatory as well. To verify the proposed method, a trajectory generation method for the dual quaternion B-spline is developed. Simulations and experiments on a five-axis machine tool are conducted, and the results demonstrate the feasibility and efficiency of the proposed method.

Electrochemical removal of nitrogenous species for high salinity contained industrial wastewater effluent

Jing Ding,Guanshu Zhao,Qingliang Zhao,Fanyu Meng 대한환경공학회 2019 대한환경공학회 학술발표논문집 Vol.2019 No.-

Synthesis of PdP Nanoparticles as Electrocatalysts for Hydrogen Evolution Reaction in an Alkaline Medium

Ming Zhao,Ming Zhao,Bin Tong,Zinan Kang,Yanfang Wu,Wei Ding,Baoming Feng 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.5

The synthesis of PdP nanoparticles (NPs) using triphenylphosphine as a phosphorus precursor was investigated. The electrocatalytic performance of PdP NPs in hydrogen evolution reaction in an alkaline medium was measured. It can be concluded that when the current density is 10 mA cm -2, the overpotential produced by Pd3P0.95 NPs is 75mV which is 185mV lower than that produced by commercial Pd/C. The mass activity of Pd3P0.95 NPs is more than twice as high as that of commercial Pd/C. The work indicates that PdP nanomaterials could be a potential catalyst for water splitting.

LncRNA H19/miR-29b-3p/PGRN Axis Promoted Epithelial-Mesenchymal Transition of Colorectal Cancer Cells by Acting on Wnt Signaling

Ding, Dayong,Li, Changfeng,Zhao, Tiancheng,Li, Dandan,Yang, Lei,Zhang, Bin Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.5

This investigation was aimed at working out the combined role of lncRNA H19, miR-29b and Wnt signaling in the development of colorectal cancer (CRC). In the aggregate, 185 CRC tissues and corresponding para-carcinoma tissues were gathered. The human CRC cell lines (i.e. HT29, HCT116, SW480 and SW620) and normal colorectal mucosa cell line (NCM460) were also purchased. Si-H19, si-NC, miR-29b-3p mimics, miR-29b-3p inhibitor, si-PGRN and negative control (NC) were, respectively, transfected into the CRC cells. Luciferase reporter plasmids were prepared to evaluate the transduction activity of $Wnt/{\beta}-catenin$ signaling pathway, and dual-luciferase reporter gene assay was arranged to confirm the targeted relationship between H19 and miR-29b-3p, as well as between miR-29b-3p and PGRN. Finally, the proliferative and invasive capacities of CRC cells were appraised through transwell, MTT and scratch assays. As a result, overexpressed H19 and down-expressed miR-29b-3p displayed close associations with the CRC patients' poor prognosis (P < 0.05). Besides, transfection with si-H19, miR-29b-3p mimic or si-PGRN were correlated with elevated E-cadherin expression, decreased snail and vimentin expressions, as well as less-motivated cell proliferation and cell metastasis (P < 0.05). Moreover, H19 was verified to directly target miR-29b-3p based on the luciferase reporter gene assay (P < 0.05), and miR-29b-3p also bound to PGRN in a direct manner (P < 0.05). Finally, addition of LiCl ($Wnt/{\beta}-catenin$ pathway activator) or XAV93920 ($Wnt/{\beta}-catenin$ pathway inhibitor) would cause remarkably altered E-cadherin, c-Myc, vimentin and snail expressions, as well as significantly changed transcriptional activity of ${\beta}-catenin/Tcf$ reporter plasmid (P < 0.05). In conclusion, the lncRNA H19/miR-29b-3p/PGRN/Wnt axis counted a great deal for seeking appropriate diagnostic biomarkers and treatment targets for CRC.

Over-expression of phosphoenolpyruvate carboxylase cDNA from C4 millet (Seteria italica) increase rice photosynthesis and yield under upland condition but not in wetland fields

Ding, Zai-Song,Huang, Su-Hua,Zhou, Bao-Yuan,Sun, Xue-Fang,Zhao, Ming 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.2

Phosphoenolpyruvate carboxylase (PEPC) catalyzes the initial fixation of $CO_2$ in $C_4$ plants. Under the control of the rice Rubisco small subunit promoter, cDNA of a $C_4$ SiPPC gene cloned from Seteria italica was introduced into Japonica rice by Agrobacterium-mediated transformation. Integration of the gene was confirmed by PCR analysis. RT-PCR showed expression of the gene at the RNA level in transgenic plants, and enzyme activity measurements confirmed the increase in PEPC protein. The transformants showed improvements in both photosynthesis rate and yield only under upland field cultivation. The possible function of PEPC in rice stress tolerance is discussed.

Interdecadal change in the lagged relationship between the Pacific–South American pattern and ENSO

Ding, R.,Li, J.,Tseng, Y. h.,Ha, K. J.,Zhao, S.,Lee, J. Y. Springer-International 2016 Climate dynamics Vol.47 No.9

<P>A significant interdecadal change in the lagged relationship between the austral summer Pacific-South American (PSA) pattern and the El Nio-Southern Oscillation (ENSO) in the following austral summer (the PSA serving as a precursor signature to ENSO events) has been detected by analysis of a 91-year historical record. Strong correlations between the PSA and ENSO occurred during the periods 1956-1975 and 1990-2004 [referred to as the high correlation (HC) periods], but the correlations were weak for the periods 1928-1956 and 1975-1990 [referred to as the low correlation (LC) periods]. Both the processes of surface air-sea coupling in the extratropical/tropical Pacific, and subsurface ocean temperature evolution along the equator associated with the PSA, were found to be stronger during the HC periods than during the LC periods, thereby resulting in a stronger influence of the PSA on the subsequent ENSO during the HC periods. Changes in the PSA-ENSO relationship can be attributed mainly to interdecadal changes in the intensity of the austral summer PSA. The latter was found to have contributions from both the modulation of the Pacific decadal oscillation and long-term variations in the Southern Annular Mode.</P>

Aberrant Expression of miR-20a and miR-203 in Cervical Cancer

Zhao, Shan,Yao, De-Sheng,Chen, Jun-Ying,Ding, Nan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4

MicroRNAs (miRNAs) are small, non-coding RNAs that are critical regulators of various diseases. MicroRNA-20a (miR-20a) and microRNA-203 (miR-203) have previously shown significant alteration in a range of cancers. In this study, the expression levels of miR-20a and miR-203 in 100 cervical cancer tissues were detected by qRT-PCR and compared to patient matched-nontumor cervical tissues. Correlations between expression level and clinicopathologic characteristics of cervical cancer were also analyzed. Finally, we studied the effect of miR-20a and miR-203 on cell proliferation in cervical cancer cell lines by MTT. We found that the expression level of miR-20a (P<0.001) was significantly higher in cervical cancer patients than in healthy controls, while that of miR-203 (P<0.001) was lower. Aberrant expression of miR-20a was correlated with lymph node metastasis (LNM), histological grade and tumor diameter, but down-regulated miR-203 was correlated with LNM only. Furthermore, we found that over-expression of miR-203 decreased cell proliferation, while reduction of miR-20a also prevented tumor progression. Our results support the involvement of miR-20a and miR-203 in cervical tumorigenesis. We propose that miRNAs might be used as therapeutic agents for cervical cancer.

RAMS evaluation for a steel-truss arch high-speed railway bridge based on SHM system

Zhao, Han-Wei,Ding, You-Liang,Geng, Fang-Fang,Li, Ai-Qun Techno-Press 2018 Structural monitoring and maintenance Vol.5 No.1

The evaluation theory of reliability, availability, maintainability and safety (RAMS) as a mature theory of state evaluation in the railway engineering, can be well used to the evaluation, management, and maintenance of complicated structure like the long-span bridge structures on the high-speed railway. Taking a typical steel-truss arch bridge on the Beijing-Shanghai high-speed railway, the Nanjing Dashengguan Yangtze River Bridge, this paper developed a new method of state evaluation for the existing steel-truss arch high-speed railway bridge. The evaluation framework of serving state for the bridge structure is presented based on the RAMS theory. According to the failure-risk, safety/availability, maintenance of bridge members, the state evaluation method of each monitoring item is presented. The weights of the performance items and the monitoring items in all evaluation levels are obtained using the analytic hierarchy process. Finally, the comprehensive serving state of bridge structure is hierarchical evaluated.