Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody

Kang-seuk Choi,Jin-ju Nah,Young-joon Ko,Shien-young Kang,Yi-seol Joo 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal AntibodyKang-seuk Choi*, Jin-ju Nah, Young-joon Ko, Shien-young Kang1 and Yi-seok JooNational Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea1Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, KoreaReceived April 2, 2003 / Accept July 10, 2003J. Vet. Sci. (2003), 4(2), 167-173JOURNAL OFVeterinaryScience*Corresponding author: Kang-seuk Choi National Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea Tel: +82-31-467-1860, Fax: +82-31-449-5882 E-mail: choiks@nvrqs.go.kr

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

대농갱이 암,수의 성장 차이

강언종,이배익,조미영,손상규,김광석,김이청,방인철 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.4

대농갱이는 동자개과의 담수어로 고부가가치를 창출 할 수 있는 새로운 담수 양식 대상종으로 주목받고 있으나 현재까지 성장도 등 양식에 대한 기초정보가 밝혀지지 않았으며, 본 연구에서는 인공으로 생산한 자 ? 치어를 사육하면서 암 ? 수의 성장 차이를 비교 ? 조사하였다. 암수의 정확한 성장 차이를 구명하기 위해 본 실험은 자어 초기 조직검사에 의한 조사와 치어기부터 표지법에 의한 조사로 구분하여 실시하였다. 조직학적 판별에 의한 암수간 성장비교 시험 결과 부화 후 50일까지는 수컷과 암컷의 성장차이는 없는 것으로 나타났으나, 부화 80일 후에는 수컷(체장 72.0±6.7㎜, 체중 3.71±6.7g이 암컷(체장 64.3±6.7㎜, 체중 3.71±0.99g)보다 성장이 빠른 것으로 나타나 대농갱이는 부화 후 50일에서 80일 사이에 암 ? 수의 성장차이가 시작되는 것으로 사료되었다. 부화 80일 이후 조사는 표지법이 개체 성장에 미치는 영향을 조사한 후 본 실험에 착수하였으며 표지구는 대조구와 사이에 유으적인 성장의 차이를 보이지 않았다. 개체간 성장차이는 조직학적 판별 결과와 동일한 경향으로 수컷이 암컷보다 성장이 유의적으로 빨랐으며(P<0.05), 시험 종료 후 암컷과 수컷의 크기는 수컷이 평균 체장 17.57㎝, 체중 52.19g으로 암컷의 평균 체장 11.02㎝, 체중 16.26g보다 체장은 약 1.5배, 체중은 약 3배이상 큰 것으로 나타났다(p=0.05). 체중으로 본 성장곡선은 지수성장을 보였으며, 수컷은 Y=0.0024X^(1.738)(R^(2)=0.985), 암컷은 Y=0.0405X^(1.0316)(R^(2)=0.983)의 지수식에 밀접하게 수렵되었고 성장의 차이는 사육기간이 길어질수록 크게 나타났다. The Ussurien bullhead, Leiocassis ussuriensis, of the family Bagridae has recently been targeted for freshwater culture in Korea. In this paper we investigated sexual dimorphism in body size in rearing condition using specimens produced artificially. Tissue sections of gonads were made between 20 and 80 days after hatching to identify sex of the specimens investigated. A difference in body size between sexes was not observed in fry until they were 50 days old. Male fry 80 days old had attained 81.62f7.21 ㎜ total length, and females 72.84±8.62 ㎜ total length. After 80 days the difference in body size was analysed by tagging. Applying the tag by cutting fin tips had no affect on the growth of the fishes (P< 0.05). The growth in body size of males was significantly faster (P< 0.05) than in females, and males attained about 1.5 times more in standard length and about 3 times more in body weight than females at the end of 320 days. The growth curve of body weight was exponential and the body weight was expressed by Y = 0.0024X^(1.738) (R² = 0.985) and Y = 0.0405X^(1.0316)(R² = 0.983) in male and female, respectively, observed at monthly intervals.

상황이야기 중재효과에 대한 메타분석

이성용,강영택,이정현 국립특수교육원 2012 특수교육연구 Vol.19 No.1

상황이야기 중재는 자폐증 및 발달장애학생의 사회적 기술과 지식을 증가하기 위하여 활용되는 효과적인 교수전략이다. 본 연구는 상황이야기를 중재로 활용한 국내 실험연구들을 메타분석 함으로써, 상황이야기 중재의 효과성을 검증하고 앞으로 효과적인 사회성 기술 교육 프로그램을 개발 및 적용하는 데 필요한 기초 자료를 제시하고자 하였다. 이를 위해 지금까지 국내에서 발표된 상황이야기 중재와 관련된 논문을 조사하고, 분석 논문 선정 기준에 따라 국내 13편의 실험 논문을 최종 분석 대상으로 선정하였다. 본 연구의 메타분석은 3가지 분석 영역에 따라 총 7개의 분석변인(연구대상, 실험환경, 실험설계, 중재유형, 목표기술, 중재효과, 유지 및 일반화 효과)으로 분석하였다. 우선 메타분석의 결과를 살펴보면 상황이야기 중재효과는 전체적으로 중간 크기의 효과를 보였으며, 유지와 일반화는 큰 효과가 있었다. 본 연구의 분석결과는 7개의 분석변인에 따라 구체적으로 제시하였으며, 그 결과는 상황이야기 중재와 효과적인 교수기법을 의미하는 근거기반 실제의 관점에서 논의하였다. The social story is effective intervention strategies for addressing social communication skills, social interaction skills, and behavioral functioning in children with ASD and developmental disabilities. The purpose of this study was to meta-analysis the research literature of social story interventions of children with ASD and developmental disabilities and to provide information and suggestions on social story interventions for future research and practices at schools. A systematic search of the literature from 1993 to 2011 revealed 13 experimental studies published in special education within South Korea. This study analyzed the 13 selected studies in terms of 7 analytical variables: participants, research settings, experimental design, target skills, type of intervention, effects of intervention, maintenance and generation. In particular, results indicated a moderately positive effect for social intervention (mean ES ≤ .80, median PND = 85%). Maintenance (median PND = 100%) and generalization effects (median PND = 100%) revealed large effects. The findings of this study were described in detail based on the 7 analytic variables. The implications of the findings and recommendations for practice and future research on social story interventions were presented.

알코올리즘 환자에서 뇌교외수초용해증과 연관된 주기교대눈떨림과 주기교대주시편위

강성원,이명제,정주리,김영진,양현덕 원광대학교 의과학연구소 2009 圓光醫科學 Vol.24 No.1

주기교대눈떨림와 주기교대주시편위는 이상안구 운동에서도 흔히 관찰되지 않는다. 이런 안구운동은 뇌교와 소뇌에 병변이 있을 때 나타난다고 보고되고 있다. 50세 남자 환자가 과음을 하고 난 이후 심하게 구토를 하여 입원하였다. 다음날 아침에 의식이 없었으며 주기교대눈떨림과 주기교대주시편위가 관찰되었다. 뇌자기공명영상에서는 뇌교나 소뇌에 병변없는 교뇌외수초용해증에 합당한 소견을 보였다. 저자는 아마도 교뇌외수초용해증이 소뇌와 전정신경핵의 경로중에 GABA 시스템에 이상이 발생하여 주기교대눈떨림과 주기교대 주시편위가 나타났을 것으로 추정한다. Background; Periodic alternating gaze deviation(PAGD) and periodic alternating nystagmus(PAN) are rare forms of abnormal eye movement. Multiple lesions in the cerebellum and brainstem have been reported with PAN and PAGD. Case Report; A 50-year-old man presented with severe vomiting after binge drinking. The next morning he. became vegetative state and showed PAGD and PAN. Brain MRI showed findings compatible with extrapontine myelinolysis(EPM) without any brainstem or cerebellar lesions. Conclusions; We postulate that the disturbed GABAnernic system in the pathway from the cerebellum to vestibular nuclei caused probably by EPM developed PAGD and PAN.

정상보행시 체중심의 수직 가속도 특성

이진복,강성재,김영호 한국전문물리치료학회 2002 한국전문물리치료학회지 Vol.9 No.3

In this study, vertical acceleration of center of mass was observed along normal gait phases in 9 healthy male volunteers (aged 25.7±2.18). The developed wireless accelerometric device was attached on the intervertebral space between L3 and L4 using a semi-elastic waist belt. A three-dimensional motion analysis system, synchronized with the accelerometry, was used for detecting gait phases. There was no significant correlation between the body weight and the acceleration. The first peak curve covered loading response phase. The second downward peak point was matched accurately with the opposite toe-off. In mid-stance and terminal stance, the acceleration curve highly resembled the vertical ground reaction force curve. There was no significant difference in timing between the final upward peak point and the initial contact. Therefore, the developed accelerometry system would be helpful in determining determine temporal gait patterns in patients with gait disorders.

한국산야생등줄쥐(Apodemus agrarius coreae)의 해마복합체에서 neuropeptide Y 면역반응세포에 관한 연구

김무강,송치원,이경열,박일권,정영길,이강이,김종섭 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

Neuropeptide Y(NPY) was first isolated from porcine brain. This discovery has lead some workers to study the distribution of this peptide in the central nervous system of various mammals. In this study the distribution pattern of neuropeptide Y-immunoreactive neurons was examined in the hippocampal complex of Striped field mouse in Korea(Apodemus agrarius coreae) Experimental animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through the left ventricle and the ascending aorta. The brains were removed and transferred 10%, 20% and 30% sucrose solution. Sections were cut on a cryostat into 40㎛-thick. The tissue was immunostained with free-floating and avidin-biotm complex(ABC) method. The results obtained in this study were summarized as followings. 1. The most of NPY-IR neurons in hippocampus proper were found in the polymorphic layer and a few was found in the pyramidal layer. 2. The process run vertically in the deep polymorphic layer and run parallel to corpus callosum in superficial polymorphic layer. 3. The shapes of neurons in hippocampus proper were varied of polymorphic, oval or round, and the nerve fibers were found in the molecular laver and the hippocampal fissure. 4. The highest density in number of NPY-IR neurons on dentate gyrus were found in hilar area of dentate gyrus. 5. The most of NPY-IR nerve fibers were found in molecular cell layer.

청경채의 엽 형질에 대한 유전분석 : Brassica campestris L. ssp. chinensis Jusl

김영호,이병군,강대성 한경대학교 2005 論文集 Vol.37 No.-

In order to obtain the genetic information on some leaf characters in pak-choi(Brassica campestris L. ssp. chinensis Jusl), diallel cross was conducted using 9 cultivars, and F1, F2 and BC1F1 hybrid lines were developed. Qualitative and quantitative genetic characters related to the three cropping patterns, spring at field, spring in polyethylene film house, and fall at field, were investigated. The results are as follows: 1. A major element related to the expression of leaf green color, and the complementary gene affecting the leaf color at the stage of pigment development were supposed to exist. 2. Heredity of leaf shape was controlled by one major gene that partially dominant, and the oval type was expressed as a dominant at crossing of oval(CR-Cheongdo) with round(Bangjucheong), oval(CR-Cheongdo) with round(Choseonghwakyeong). 3. Inheritance of plant types, erect, semi-erect, and spread, was affected by single gene and the expression of dominant effects increased in order of erect>semi-erect>spread. 4. Heterosis of leaf length, leaf width, midrib length, midrib width and midrib thickness was highly expressed in all cropping patterns. Some characters such as leaf length, number of leaves, midrib length, midrib width, midrib thickness and fresh weight per plant showed significantly positive heterosis at the spring field cropping. The same effect was observed in leaf length, leaf width, midrib length and midrib thickness at the spring in polyethylene film house cropping, and in leaf length, leaf width, leaf shape index, midrib length, midrib width, midrib thickness and fresh weigth per plant at the field cropping in fall. 5. Estimated as a mean heterosis and a heterobeltiosis effects(%), an increase in fresh weigth per plant was attributed to the increase of midrib thickness, midrib width, midrib length, leaf length and leaf width characters. These effects were more significant at the field cropping in fall than spring cropping. Also, hybrid lines showed more late bolting character compared to their parents.

어머니가 지각한 국민학교 고학년 아동의 문제행동에 관한 연구 : 대전시내 일부 국민학교를 대상으로 Using a Selected Group of Several Elementary School in Taejon City

이기영,박인숙,이강이 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

The purpose of this thesis was to study children's problem behaviors and to represent the references and the useful data for successful child rearing, by observing children's problem behaviors and their emotional inadaptation focused on a number of environmental factors, and by examining many factors which have an effect on children's problem behaviors. Data for research were sampled from 264 mothers of fifth and sixth grade children attending two elementary schools on Taejon city Data were collected by posing a questions method duping the period July 2-July 7 in 1990. The instrument used in the study was the Hong Kyeong Ja's child behavior profile and, through the SPSS progrom, the collected data were analysed into Frequency, ANOVA, Pearson Correlation coefficient. The Conclusions were as follows : 1. Factors affecting children's problem behaviors 1) Father's occupation : Physical laborer's children recorded a high problem behavior index point in psychotic, hyperactivity, paranoid(P<.05), whereas office workers children got a high problem behavior index point in delinquency (P<.015), immature aggression (P<.05) : The result is statistically significant 2) Father's educational level : The children whose fathers had a level of education lower than middle school level were marked by high problem behavior index point in psychotic, immature aggression, delinquency, hystery. sexual problem, hyperactivity(P<.001), paranoid(P<.01), superiority(P<.05) 3) Monthly income : The children whose families earned a lower income than 550,000 won a month recorded a high problem behavior index point in immature aggression (P<.001.), psychotic, paranoid(P<.05) : the results are statistically significant. 4) Mother's occupation : It turns out that whether the children's mother had a job or not didn't make any meaningful difference to children's problem behavior through 14 items. 5) Mother's educational level The children whose mother had a level of education lower than middle school level marked a high problem behavior index point in psychotic, immature aggression, hystery, sexual problems, hyperactivity, paranoid(P<.001), deliquency(P<.01), superiority, obsession(P<.05): the results is statistically significant 6) Differences between the male children and female children : Male children had more meaningful differences in hystery. sexual problems(P>.05), whereas female children had more significant differences in social withdrawal(P<.01). 7) Problem behavior on the basis of father's educational level The male children, whose father had a level of education lower than primary school level, recorded a high problem behavior index point in psycotic, overanxiety, immature aggression(P<.001), superiority, social withdrawal, delinquency, hvperactivity(P<.01), psychosomatic, obsession, emotional instability, hystery·sexual problem, paranoid(P<.05), whereas the female children whose father had a level of education lower than middle school level marked a high problem behavior index point in hystery·sexual problem(P<.01), hyperactivity(P<.05). 8) Problem behavior on the basis of family monthly income : The male children, whose family got a lower income than 550,000won a month, recorded a high problem behavior index point in psychotic, overanxiety, immature aggression(P<.05), whereas female children marked a high problem behavior index point in immature aggression, hystery·sexual problem, hyperactivity(P<.05) : the result is statistically 9) Problem behavior on the basis of mother's educational significamt. The male children whose mother had a level of education lower than middle school level, marked a high problem behavior index point in psychotic(P<.001), immature aggression, hystery·sexual problem(P<.01), superiority, obsession, overanxiety, delinguency, hyperactivity, paranoid (P<.05), whereas the female children recorded a high problem behavior index point in hystery·sexual problem(P<.001), hyperactivity, paranold(P<.01), delinquency(P<.05) 2. Relationships between mother's rearing attitude and children's problem behavior 1) According to the standard based on the mother's affection and love for the first 5 years, the child who had had lack of affection and love marked a high problem behavior index point in psychosomatic, social withdrawal, psychotic, paranoid(P<.001), superiority, overanxiety, immature aggression(P<.01), emotional instability, hystery. sexual problem, hyperactivity(P<.05): the result is statistically significant. 2) According to innate nature, unmeek children recorded a high problem behavior index point in superiority, overanxiety, immature aggression, hyperactivity, paranoid(P<.001), regression (P<.01), Psychosomatic(P<.05). 3) According to the rearing attitude, through interference marked a high problem behavior index point in paranoid(P<.001), psychotic, immature aggression(P<.01), superiority(P<.05), and considerable interference recorded high problem behavior index point in social withdrawal, overanxietv(P<.01). 3. The correlation between problem behavior variables of each item turned out to be a positive correlationship.

가역적 후외동맥 폐색으로 발현된 전조편두통 연관 뇌경색

김영진,이명제,정주리,강성원,양현덕 원광대학교 의과학연구소 2009 圓光醫科學 Vol.24 No.1

예전 인구에 근거하여 연구된 논문들은 편두통이 뇌졸중의 위험성을 높일 수 있다는 보고를 하였으며, 전조증상을 동반한 편두통은 중추신경계와 연관된 병인론적 과정을 통하여 뇌졸중과의 관련성이 있다는 보고가 있다. 그러나, 편두통성 뇌졸중의 병태생리는 아직 정확히 밝혀지지 않았다. 편두통의 발생 원인에서 혈관성 이론은 편두통을 혈관수축으로 발생하는 전조증상과 함께 대뇌 조절 장애로 발생한다는 이론으로 뇌허혈증상은 일으킬 수 있다. 전조증상을 동반한 편두통성 뇌경색은 허혈성 뇌졸중의 드문 원인이며, 또한 편두통과 허혈성 뇌졸중사이의 관련성은 아직 논란으로 남아 있다. 우리는 혈관경련 수축으로 나타날 수 있는 전조성 편두통과 관련된 뇌경색에서의 가역적 후뇌 동맥 폐색 1례를 보고한다. Several previous population-based studies reported an increase risk of stroke in association with migraine. Migraine with aura increased stroke risk with pathogenetic process in the central nervous system. However, the pathologic mechanism of migrainous infarction remains elusive. The vasogenic theory ascribes migraine to cerebral dysregulation with aura resulting from vasoconstriction. Migrainous infarction with aura is a rare cause of cerebral ischemic stroke. Also, the relationship between migraine and ischemic stroke remains controversial. We report a case of cerebral infarction associated with migraine with aura that shows reversible arterial narrowing which might be caused by vasospasm.