http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Yaohua You (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Characterization of quercetin and its glycoside derivatives in Malus germplasm
Lei Zhang,Qipeng Xu,Yaohua You,Weifeng Chen,Zhengcao Xiao,Pengmin Li,Fengwang Ma 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.6
Quercetin and its glycoside derivatives were identifi ed and quantifi ed using high-performance liquid chromatograph (HPLC)and liquid chromatograph/mass spectrometer/mass spectrometer (LC/MS/MS) in the leaves, flowers, and fruits of 22 Malusgenotypes. In all genotypes, small amounts of quercetin aglycone were present, with water-soluble glycoside forms were themost abundant in diff erent Malus plant tissues, including quercetin-3-galactoside, quercetin-3-rutinoside, quercetin-3-glucoside,quercetin-3-xyloside, quercetin-3-arabinoside, and quercetin-3-rhamnoside. Among these six quercetin glycosides,quercetin-3-galactoside was the common form in Malus plants, except in the leaves and flowers of M. ceracifolia and M. magdeburgensis , and in the fruits of M. micromalus ‘Haihong Fruit’, where there was a higher concentration of quercetin-3-glucoside. Among the diff erent tissues tested, leaves contained the highest concentration of quercetin and its glycosides,while fruits contained the lowest concentrations of these compounds. Among the genotypes we analyzed, no specifi c genotypeconsistently contained the highest concentration of quercetin and its glycoside derivatives. M. domestica ‘Honeycrisp’had the highest total compound concentration (approximately 1600 mg kg −1 ), whereas M. hupehensis contained the lowestin its fruits. In contrast, the concentration of total quercetin and its glycosides was more than 5000 mg kg −1 in the leaves ofeight genotypes and greater than 2500 mg kg −1 in the flowers of seven species. In general, the concentration of quercetinand its glycoside derivatives depended on the species and tissue type. These results may provide useful information for theevaluation and selection of edible Malus fruits and the materials for quercetin glycoside extraction.
-
Rana Naveed Ur Rehman,Yaohua You,Chengquan Yang,Abdul Rehman Khan,Pengmin Li,Fengwang Ma 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.4
The red coloration of young crabapple (Malus orientalis) flowers results from their high cellular concentrationof anthocyanins. As the petals grow larger, the color fades, which is commonly regarded as being caused by floraltissue expansion diluting the anthocyanin levels in larger cells. We hypothesized that the anthocyanins are degradeddue to hydrogen peroxide (H2O2) and that the concentrations of each phenolic intermediate depends on the physiologicalgrowth of flower petals. A high-performance liquid chromatography (HPLC) analysis was performed to characterizethe identities and concentrations of the diverse array of phenolics present during different growth stages in the crabappleflowers. The concentrations of gallic acid, caffeic acid, syringic acid, chlorogenic acid, phloridzin, procyanidin B1,procyanidin B2, and catechin significantly increased throughout development, while epicatechin and cyanidin-3-galactosidelevels declined. Most flavonols (quercetin-3-xyloside, quercetin-3-rhaminose, quercetin-3-glucoside, and quercetin-3-arabinoside)increased until the fourth stage, after which they declined suddenly. Likewise, cyanidin-3-galactoside degraded sharplyat stage III, exhibiting reciprocal relationship with H2O2 concentration. Our results suggest that the concentrations ofphenolic compounds are congruent with their functional activities in floral tissues, and that their systematicfluctuation mainly depends on environmental cues and physiological imbalances, not merely petal expansion. Theresults of this study will be useful for better understanding the physiological changes in phenolic content andanthocyanin degradation that occur during flower development.
-
Rana Naveed Ur Rehman,Yaohua You,Sajid Ali,Yule Wang,Lei Zhang,Pengmin Li,Fengwang Ma 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.1
Polyphenol intermediates accumulate predominantly in peripheral parts of floral tissues and play various roles in tissuehomeostasis. A limited ornamental flower shelf life is caused by early tissue senescence, otherwise known as programmedcell death (PCD), which is characterized by processes such as DNA degradation, reduction in protein content and others. However, the role of polyphenols during PCD is poorly understood. In this study, we evaluated polyphenols as potentialbiochemical markers during developmental changes associated with PCD. Malus crabapple flowers were exposed to low(LT = 13 ± 2 °C), room (RT = 23 ± 2 °C), and high (HT = 30 ± 2 °C) temperatures to induce different levels of PCD. HTtreatment was associated with enhanced H2O2and MDA content, which subsequently caused lower protein concentrationand induced DNA degradation. Conversely, substantially higher protein content, lesser DNA degradation, and lower H2O2and MDA accumulation were observed following RT and LT treatments. Furthermore, significantly higher concentrationsof phenolic acids, flavanols, and anthocyanins were observed following both RT and LT treatments, more so for the latter,due to up-regulation of structural genes such as MpPAL, MpDFR, MpLDOX, and MpUFGT. Similarly, following HT treatmentthat induced a higher rate of PCD, the accumulation of dihydrochalcone and flavonols was significantly enhanced andassociated with increased expression of MpCHS and MpFLS. Phenolic contents are determined via precise regulation of ahighly conserved set of structural genes, indicating their functional importance. Therefore, it was assumed that individualphenolic intermediates or discrete diverse classes of polyphenol compounds may be utilized as biochemical markers of PCDin woody ornamental flowers of Malus crabapple.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
