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        Characterization of Phenolic Compounds and Active Anthocyanin Degradation in Crabapple (Malus orientalis) Flowers

        Rana Naveed Ur Rehman,Yaohua You,Chengquan Yang,Abdul Rehman Khan,Pengmin Li,Fengwang Ma 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.4

        The red coloration of young crabapple (Malus orientalis) flowers results from their high cellular concentrationof anthocyanins. As the petals grow larger, the color fades, which is commonly regarded as being caused by floraltissue expansion diluting the anthocyanin levels in larger cells. We hypothesized that the anthocyanins are degradeddue to hydrogen peroxide (H2O2) and that the concentrations of each phenolic intermediate depends on the physiologicalgrowth of flower petals. A high-performance liquid chromatography (HPLC) analysis was performed to characterizethe identities and concentrations of the diverse array of phenolics present during different growth stages in the crabappleflowers. The concentrations of gallic acid, caffeic acid, syringic acid, chlorogenic acid, phloridzin, procyanidin B1,procyanidin B2, and catechin significantly increased throughout development, while epicatechin and cyanidin-3-galactosidelevels declined. Most flavonols (quercetin-3-xyloside, quercetin-3-rhaminose, quercetin-3-glucoside, and quercetin-3-arabinoside)increased until the fourth stage, after which they declined suddenly. Likewise, cyanidin-3-galactoside degraded sharplyat stage III, exhibiting reciprocal relationship with H2O2 concentration. Our results suggest that the concentrations ofphenolic compounds are congruent with their functional activities in floral tissues, and that their systematicfluctuation mainly depends on environmental cues and physiological imbalances, not merely petal expansion. Theresults of this study will be useful for better understanding the physiological changes in phenolic content andanthocyanin degradation that occur during flower development.

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