Phenolic compounds as biochemical markers of senescence in woody ornamental flowers of Malus crabapple

다국어 초록 (Multilingual Abstract)

Polyphenol intermediates accumulate predominantly in peripheral parts of floral tissues and play various roles in tissuehomeostasis. A limited ornamental flower shelf life is caused by early tissue senescence, otherwise known as programmedcell death (PCD), which is characterized by processes such as DNA degradation, reduction in protein content and others.
However, the role of polyphenols during PCD is poorly understood. In this study, we evaluated polyphenols as potentialbiochemical markers during developmental changes associated with PCD. Malus crabapple flowers were exposed to low(LT = 13 ± 2 °C), room (RT = 23 ± 2 °C), and high (HT = 30 ± 2 °C) temperatures to induce different levels of PCD. HTtreatment was associated with enhanced H2O2and MDA content, which subsequently caused lower protein concentrationand induced DNA degradation. Conversely, substantially higher protein content, lesser DNA degradation, and lower H2O2and MDA accumulation were observed following RT and LT treatments. Furthermore, significantly higher concentrationsof phenolic acids, flavanols, and anthocyanins were observed following both RT and LT treatments, more so for the latter,due to up-regulation of structural genes such as MpPAL, MpDFR, MpLDOX, and MpUFGT. Similarly, following HT treatmentthat induced a higher rate of PCD, the accumulation of dihydrochalcone and flavonols was significantly enhanced andassociated with increased expression of MpCHS and MpFLS. Phenolic contents are determined via precise regulation of ahighly conserved set of structural genes, indicating their functional importance. Therefore, it was assumed that individualphenolic intermediates or discrete diverse classes of polyphenol compounds may be utilized as biochemical markers of PCDin woody ornamental flowers of Malus crabapple.

참고문헌 (Reference)

1 Diaz-Tielas C, "The natural compound trans-chalcone induces programmed cell death in Arabidopsis thaliana roots" 35 : 1500-1517, 2012

2 Koca N, "The effects of plant growth regulators and L-phenylalanine on phenolic compounds of sweet basil" 166 : 515-521, 2015

3 Tian J, "The balance of expression of dihydroflavonol 4-reductase and flavonol synthase regulates flavonoid biosynthesis and red foliage coloration in crabapples" 5 : 12228-, 2015

4 Jiang R, "The Malus crabapple transcription factor McMYB10 regulates anthocyanin biosynthesis during petal coloration" 166 : 42-49, 2014

5 Sun S, "Response of phenolic compounds in 'Golden Delicious' and 'Red Delicious' apples peel to fruit bagging and subsequent sunlight re-exposure" 168 : 161-167, 2014

6 Zhang J, "Reactive oxygen species produced via plasma membrane NADPH oxidase regulate anthocyanin synthesis in apple peel" 240 : 1023-1035, 2014

7 Gill SS, "Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants" 48 : 909-930, 2010

8 Karuppagounder SS, "Quercetin up-regulates mitochondrial Complex-I activity to protect against programmed cell death in rotenone model of parkinson's disease in rats" 236 : 136-148, 2013

9 Oke F, "Protective effect of two edible mushrooms against oxidative cell damage and their phenolic composition" 128 : 613-619, 2011

10 Mochizuki-kawai H, "Programmed cell death begins earlier in the mesophyll cells of tulip petals than in the epidermal cells" 79 : 9-12, 2013

11 Zhang XZ, "Potential polyphenol markers of phase change in apple (Malus domestica)" 164 : 574-580, 2007

12 Fernandez-Lorenzo JL, "Polyphenols as potential markers to differentiate juvenile and mature chestnut shoot cultures" 19 : 461-466, 1999

13 Ding J, "Physiological basis of different allelopathic reactions of cucumber and figleaf gourd plants to cinnamic acid" 58 : 3765-3773, 2007

14 Gosch C, "Phloridzin: biosynthesis, distribution and physiological relevance in plants" 71 : 838-843, 2010

15 Gornas P, "Phenolic compounds in different fruit parts of crab apple: dihydrochalcones as promising quality markers of industrial apple pomace by-products" 74 : 607-612, 2015

16 Azad AK, "Intracellular energy depletion triggers programmed cell death during petal senescence in tulip" 59 : 2085-2095, 2008

17 Shaked-Sachray L, "Increased anthocyanin accumulation in aster flowers at elevated temperatures due to magnesium treatment" 114 : 559-565, 2002

18 Zou J, "Increase in DNA fragmentation and the role of ethylene and reactive oxygen species in petal senescence of Osmanthus fragrans" 93 : 97-105, 2014

19 Zipor G, "In planta anthocyanin degradation by a vacuolar class III peroxidase in Brunfelsia calycina flowers" 205 : 653-665, 2014

20 Gao C, "Implication of reactive oxygen species and mitochondrial dysfunction in the early stages of plant programmed cell death induced by ultraviolet-C overexposure" 227 : 755-767, 2008

21 Muthuswamy S, "Fruit phenolics as natural antimicrobial agents: selective antimicrobial activity of catechin, chlorogenic acid and phloridzin" 5 : 81-85, 2007

22 Bertolini A, "Flavonoids and darkness lower PCD in senescing Vitis vinifera suspension cell cultures" 16 : 233-, 2016

23 Russo M, "Flavonoid quercetin sensitizes a CD95-resistant cell line to apoptosis by activating protein kinase $C{\alpha}$" 22 : 3330-3342, 2003

24 Fico G, "Flavonoid distribution in Pyracantha coccinea plants at different growth phases" 28 : 673-678, 2000

25 Heim KE, "Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships" 13 : 572-584, 2002

26 Pannala AS, "Flavonoid B-ring chemistry and antioxidant activity: fast reaction kinetics" 282 : 1161-1168, 2001

27 Pontais I, "Erwinia amylovora modifies phenolic profiles of susceptible and resistant apple through its type III secretion system" 132 : 262-271, 2008

28 Bais HP, "Enantiomeric-dependent phytotoxic and antimicrobial activity of (${\pm}$)-catechin. A rhizosecreted racemic mixture from spotted knapweed" 128 : 1173-1179, 2002

29 Baziramakenga R, "Effects of benzoic and cinnamic acids on membrane permeability of soybean roots" 21 : 1271-1285, 1995

30 Perry LG, "Dual role for an allelochemical: (${\pm}$)-catechin from Centaurea maculosa root exudates regulates conspecific seedling establishment" 93 : 1126-1135, 2005

31 Xue H, "Differences in salt tolerance between diploid and autotetraploid apple seedlings exposed to salt stress" 190 : 24-30, 2015

32 Mansouri A, "Determination of hydrogen peroxide scavenging activity of cinnamic and benzoic acids employing a highly sensitive peroxyoxalate chemiluminescence-based assay: structure-activity relationships" 39 : 22-26, 2005

33 Vacca RA, "Cytochrome c is released in a reactive oxygen speciesdependent manner and is degraded via caspase-like proteases in tobacco bright-yellow 2 cells en route to heat shock-induced cell death" 141 : 208-219, 2006

34 Rana Naveed Ur Rehman, "Characterization of Phenolic Compounds and Active Anthocyanin Degradation in Crabapple (Malus orientalis) Flowers" 한국원예학회 58 (58): 324-333, 2017

35 Claudot A, "Chalcone synthase activity and polyphenolic compounds of shoot tissues from adult and rejuvenated walnut trees" 203 : 275-282, 1997

36 Bi X, "Anthocyanin contributes more to hydrogen peroxide scavenging than other phenolics in apple peel" 152 : 205-209, 2014

37 Fang F, "An intracellular laccase is responsible for the epicatechin mediated anthocyanin degradation in litchi fruit pericarp" 169 : 2391-2408, 2015

38 Bradford MM, "A rapid and sensitive method for the quantitation microgram quantities of protein utilizing the principle of proteindye binding" 254 : 248-254, 1976