http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Zou Yi,Changyou Han,Fang Wang,Yanhua Tan,Saina Yang,Chuying Huang,Shengyou Xie,Xueqin Xiao 한국식물학회 2021 Journal of Plant Biology Vol.64 No.2
Aloe vera L. is an excellent resource for medication. Selenium-enriched aloe can act as a functional food to human health. To understand the molecular mechanisms underlying selenium accumulation in Aloe vera L., we characterized the metabolic and transcriptome responses of aloe leaves under different Na2SeO4 levels (0, 200, and 400 mg/L) treatments. Aloe leaves spraying with exogenous selenium fertilizer showed a significant increase in total Se content compared with those under non-treatment control, and no distinct differences were observed between 200 and 400 mg/L Se treatment. Non-targeted metabolic profiling revealed that Se treatment triggered the accumulation of antioxidants, including amino acid and derivatives, phenols, flavonoids, terpene, as well as indole derivatives. Consistent with metabolic changes following Se treatment, the transcript level of genes involved in Se assimilation and Se-response showed dramatically increase, such as those encoding sulfate transporter, antioxidants, phytohormone signaling, transcription factors, and phenols metabolites, suggesting Se assimilation generally accompanied with antioxidant and pathogen defense. This study exhibited comprehensive insights on Se response in Aloe vera L., and provided us with targeted genes for genetic engineering, thereby improving the therapeutic value of aloe.
Assessment of the Cytotoxic and Apoptotic Effects of Chaetominine in a Human Leukemia Cell Line
Yao, Jingyun,Jiao, Ruihua,Liu, Changqing,Zhang, Yupeng,Yu, Wanguo,Lu, Yanhua,Tan, Renxiang The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2
Chaetominine is a quinazoline alkaloid originating from the endophytic fungus Aspergillus fumigatus CY018. In this study, we showed evidence that chaetominine has cytotoxic and apoptotic effects on human leukemia K562 cells and investigated the pathway involved in chaetominine-induced apoptosis in detail. Chaetominine inhibited K562 cell growth, with an $IC_{50}$ value of 35 nM, but showed little inhibitory effect on the growth of human peripheral blood mononuclear cells. The high apoptosis rates, morphological apoptotic features, and DNA fragmentation caused by chaetominine indicated that the cytotoxicity was partially caused by its pro-apoptotic effect. Under chaetominine treatment, the Bax/Bcl-2 ratio was upregulated (from 0.3 to 8), which was followed by a decrease in mitochondrial membrane potential, release of cytochrome c from mitochondria into the cytosol, and stimulation of Apaf-1. Furthermore, activation of caspase-9 and caspase-3, which are the main executers of the apoptotic process, was observed. These results demonstrated that chaetominine induced cell apoptosis via the mitochondrial pathway. Chaetominine inhibited K562 cell growth and induced apoptotic cell death through the intrinsic pathway, which suggests that chaetominine might be a promising therapeutic for leukemia.