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cis-Dichlorodiammineplatinum(Ⅱ)이 발정주기에 따른 흰쥐 난관섬모 세포의 형태 및 섬모형성에 미치는 영향에 관한 전자현미경적 및 면역조직화학적 연구
김동옥,송양주,전영희,정호삼 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.1
electron microscope for morphological changes of cytoplasmic organelles and with light microscope for detection of tubulin substances in the cytoplasm during estrous cycle. Experimental animals(weighting 200gm. female albino rats) divided into 4 groups by the estrous cycles. Ampullar oviducts of these animals were excised at each estrous cycles. The specimen was made into immunocytological reaction slides for detection of tubulin in the cytoplasm, and the other specimen were made to ultrathin section for electron microscopy. All of specimens were examined with light and electron microscope. The results obtained are as follows. 1. The tubulin substances in ciliated cells of oviducts revealed strong reactions during proestrus and estrus but weak reactions during metestus and diestrus. 2. After cis-Platin treatment, tubulin substances in the cytoplasm of oviductal ciliated cells showed weak stain reactions but at diestrus, moderate reactions were seen similar to that of the control group. 3. The cilia and basal bodies of ciliated cells of ampulla oviducts after cisplatin treatment were decreased in number at all stages of estrous cycle. 4. Ciliated cells in cis-Platin treated rat revealed hypertropies of mitochondria, atrophies of Golgi complex, decrease of polyribosomes and segmented cisternae of rough endoplasmic reticulum at proestrus, estrus and metestrus. It is consequently suggested that cis-Platin would induce inhibition of ciliogenesis in oviductal ciliated cells during estrous cycles.
박지훈,이상혁,김희,이재호,박성재,지삼룡,양성연,박은택,이연재,설상영,정정명,강미선 白中央醫療院 2005 仁濟醫學 Vol.26 No.1
Fungus such as Candida albicans is a normal flora that is frequently found in the oral cavity, gastrointestinal tract, genitourinary tract, vaginal mucosa in a normal person. However, candida can cause opportunistic infection in an immune compromised host. Candidiasis has broad spectrum of disease from mucocutaneous infection to invasive or disseminated infection. But, it is rarely reported that candida is associaed with gastrointestinal tract disease in a healthy adult. The case of gastric ulcer associated with candida particularly in a health adult is reported with relevant literature.
Jung Yoon Yang,Min Young Park,Sam Young Park,Hong Il Yoo,Min Seok Kim,Jae Hyung Kim,Won Jae Kim,Ji Yeon Jung 대한생리학회-대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6
Nitric oxide (NO) is important in the regulation of bone remodeling, whereas high concentration of NO promotes cell death of osteoblast. However, it is not clear yet whether NO-induced autophagy is implicated in cell death or survival of osteoblast. The present study is aimed to examine the role of NO-induced autophagy in the MC3T3-E1 cells and their underlying molecular mechanism. The effect of sodium nitroprusside (SNP), an NO donor, on the cytotoxicity of the MC3T3-E1 cells was determined by MTT assay and expression of apoptosis or autophagy associated molecules was evaluated by western blot analysis. The morphological observation of autophagy and apoptosis by acridine orange stain and TUNEL assay were performed, respectively. Treatment of SNP decreased the cell viability of the MC3T3-E1 cells in dose- and time-dependent manner. SNP increased expression levels of p62, ATG7, Beclin-1 and LC3-II, as typical autophagic markers and augmented acidic autophagolysosomal vacuoles, detected by acridine orange staining. However, pretreatment with 3-methyladenine (3MA), the specific inhibitor for autophagy, decreased cell viability, whereas increased the cleavage of PARP and caspase-3 in the SNP-treated MC3T3-E1 cells. AMP-activated protein kinase (AMPK), a major autophagy regulatory kinase, was activated in SNP-treated MC3T3-E1 cells. In addition, pretreatment with compound C, an inhibitor of AMPK, decreased cell viability, whereas increased the number of apoptotic cells, cleaved PARP and caspase-3 levels compared to those of SNP-treated MC3T3-E1 cells. Taken together, it is speculated that NO-induced autophagy functions as a survival mechanism via AMPK activation against apoptosis in the MC3T3-E1 cells.
Yang, Jung Yoon,Park, Min Young,Park, Sam Young,Yoo, Hong Il,Kim, Min Seok,Kim, Jae Hyung,Kim, Won Jae,Jung, Ji Yeon The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6
Nitric oxide (NO) is important in the regulation of bone remodeling, whereas high concentration of NO promotes cell death of osteoblast. However, it is not clear yet whether NO-induced autophagy is implicated in cell death or survival of osteoblast. The present study is aimed to examine the role of NO-induced autophagy in the MC3T3-E1 cells and their underlying molecular mechanism. The effect of sodium nitroprusside (SNP), an NO donor, on the cytotoxicity of the MC3T3-E1 cells was determined by MTT assay and expression of apoptosis or autophagy associated molecules was evaluated by western blot analysis. The morphological observation of autophagy and apoptosis by acridine orange stain and TUNEL assay were performed, respectively. Treatment of SNP decreased the cell viability of the MC3T3-E1 cells in dose- and time-dependent manner. SNP increased expression levels of p62, ATG7, Beclin-1 and LC3-II, as typical autophagic markers and augmented acidic autophagolysosomal vacuoles, detected by acridine orange staining. However, pretreatment with 3-methyladenine (3MA), the specific inhibitor for autophagy, decreased cell viability, whereas increased the cleavage of PARP and caspase-3 in the SNP-treated MC3T3-E1 cells. AMP-activated protein kinase (AMPK), a major autophagy regulatory kinase, was activated in SNP-treated MC3T3-E1 cells. In addition, pretreatment with compound C, an inhibitor of AMPK, decreased cell viability, whereas increased the number of apoptotic cells, cleaved PARP and caspase-3 levels compared to those of SNP-treated MC3T3-E1 cells. Taken together, it is speculated that NO-induced autophagy functions as a survival mechanism via AMPK activation against apoptosis in the MC3T3-E1 cells.
Proteomic Analysis of Resting and Activated Human CD8(+) T Cells
( Jung Hui Koo ),( Wook Jin Chae ),( Je Min Choi ),( Hyung Wook Nam ),( Tomohiro Morio ),( Yu Sam Kim ),( Yang Soo Jang ),( Kwan Yong Choi ),( Jung Jin Yang ),( Sang Kyou Lee ) 한국미생물생명공학회 2006 Journal of microbiology and biotechnology Vol.16 No.6
생체전기임피던스 측정법에 의한 경신강지환16의 비만개선 효과 평가
정양삼 ( Yang Sam Jung ),윤기현 ( Ki Hyeon Yoon ),최승배 ( Seung Bae Choi ),윤미정 ( Mi Chung Yoon ),신순식 ( Soon Shik Shin ) 대한한방신경정신과학회 2008 동의신경정신과학회지 Vol.19 No.1
Objective : Obesity threatens not only the problem of beauty but also health. Furthermore, it could be harmful a chronic disease to increase mortality rate. A purpose of this study is to show a effect of obesity control as developing a herbal medicine, Gyeongshingangjeehwan16 (GGEx16), in order to control obesity that is a harmful factor for healthy. Method : In order to prove the effect of GGEx16, BMI, fat distribution, fat control and fitness score which are closely related with obesity are considered as variables. Each variable is measured, for statistical analysis, using measurement implement of InBody 3.0 which applied a theory of bioimpedence analysis. Result and Conclusion : As a result of statistical analysis for four variables, it was improved that there are the improved effect for obesity because GGEx16 is statistically meaningful better than prior to taking.
고지방식이 비만마우스 모델에서 파키스탄산 및 중국산 마황으로 조성된 강지환(降脂丸)-1,2,3,4과 降脂丸-1+가미소체환(加味消滯丸)의 비알콜성 지방간질환 개선효과 비교
정양삼 ( Yang Sam Jung ),김종훈 ( Jong Hoon Kim ),김병출 ( Byeong Chul Kim ),석화준 ( Hoa Jun Seok ),유재상 ( Jae Sang Yoo ),구자룡 ( Ja Ryong Ku ),윤기현 ( Ki Hyeon Yoon ),조주흠 ( Ju Heum Jo ),장두현 ( Du Hyon Jang ),윤호영 ( Ho 대한본초학회 2014 大韓本草學會誌 Vol.29 No.6
Objectives : This study investigated the improvement effects of Gangjihwan-1,2,3,4 (DF-1,2,3,4) and combination of DF-1 and Gamisochehwan (GSH) on nonalcoholic fatty liver disease in a high fat diet-fed obese mouse model. Methods : Eight-week-old C57BL/6N mice were divided into eight groups: a normal lean group given a standard diet, an obese control group given a high fat diet, and atorvastatin, DF-1,2,3,4, and DF-1+GSH groups given a high fat diet with atorvastatin (10 mg/kg), DF-1,2,3,4 (40, 80, 160, 80 mg/kg), and DF-1+GSH (80 mg/kg), respectively. After 8 weeks of treatment, body weight gain, blood lipid markers, ALT concentrations, liver weight and histology were examined. Results : Body weight gain was significantly decreased in DF-1,2,3,,4, DF-1+GSH and atorvastatin groups compared with control. The extent of decreases was eminent in DF-1+GSH group. Circulating concentrations of total cholesterol and LDL-cholesterol were significantly decreased in DF-2, DF-4, DF-1+GSH and atorvastatin groups compared with control. Liver weights, hepatic lipid accumulation and hepatic fibrosis were significantly decreased in DF-1,2,3,4, DF-1+GSH and atorvastatin groups compared with control, and the magnitude of which was more effective in DF-1+GSH group than in DF-only group. Circulating ALT concentrations were significantly decreased only in DF-4 and DF-1+GSH groups. Conclusions : In conclusion, these results suggest that DF decreases body weight gain, improves blood lipid metabolism, and reduces liver weight and hepatic lipid accumulation and hepatic fibrosis, contributing to the improvement of nonalcoholic fatty liver disease. In addition, these effects were more effective in DF-1+GSH combination group than in DF-only group.