Effects of Continuous Straw Returning on Soil Functional Microorganisms and Microbial Communities

Guan Yunpeng,Wu Meikang,Che Songhao,Yuan Shuai,Yang Xue,Li Siyuan,Tian Ping,Wu Lei,Yang Meiying,Wu Zhihai 한국미생물학회 2023 The journal of microbiology Vol.61 No.1

This study examined the changes in soil enzymatic activity, microbial carbon source metabolic diversity, and straw decomposition rates in paddy fields treated with 1, 2, or 3 years of straw returning (SR1–SR3). The soil’s ability to decompose straw and cellulolytic bacteria increased with the number of treatment years (1: 31.9% vs. 2: 43.9% vs. 3: 51.9%, P < 0.05). The numbers of Azotobacter, Nitrobacteria, cellulolytic bacteria, and inorganic phosphate bacteria increased progressively with the numbers of straw returning years. Cellulolytic bacteria and inorganic phosphate bacteria were significantly positively correlated with the decomposition rate (r = 0.783 and r = 0.375, P < 0.05). Based on 16S sequencing results, straw returning improved the microbial diversity of paddy soils by increasing unclassified bacteria and keeping dominant soil microorganism populations unchanged. The relative importance of individual microbial taxa was compared using random forest models. Proteobacteria, ammoniating bacteria, and potassium dissolving bacteria contributed to peroxidase activity. The significant contributors to phosphate monoesterase were Acidobacteriota, Desulfobacterota, ammoniating bacteria, cellulolytic bacteria, and potassium-dissolving bacteria. Proteobacteria, ammoniating bacteria, cellulolytic bacteria, and potassium-dissolving bacteria contributed to urease activity. Desulfobacterota, ammoniating bacteria, cellulolytic bacteria, and potassium-dissolving bacteria contributed to the neutral invertase activity. In conclusion, soil microbial community structure and function were affected within 2 years of straw returning, which was driven by the combined effects of soil organic carbon, available nitrogen, available potassium, and pH. With elapsing straw returning years, soil properties interacted with soil microbial communities, and a healthier soil micro-ecological environment would form.

Comment on “Clinical characteristics of spontaneous coronary artery dissection in young female patients with acute myocardial infarction in Korea”

( Guan Yang Kang ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.1

A Singular Value Decomposition based Space Vector Modulation to Reduce the Output Common-Mode Voltage of Direct Matrix Converters

Guan, Quanxue,Yang, Ping,Guan, Quansheng,Wang, Xiaohong,Wu, Qinghua The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.3

Large magnitude common-mode voltage (CMV) and its variation dv/dt have an adverse effect on motor drives that leads to early winding failure and bearing deterioration. For matrix converters, the switch states that connect each output line to a different input phase result in the lowest CMV among all of the valid switch states. To reduce the output CMV for matrix converters, this paper presents a new space vector modulation (SVM) strategy by utilizing these switch states. By this mean, the peak value and the root mean square of the CMV are dramatically decreased. In comparison with the conventional SVM methods this strategy has a similar computation overhead. Experiment results are shown to validate the effectiveness of the proposed modulation method.

Positive Association of TEAD1 With Schizophrenia in a Northeast Chinese Han Population

Yang Sun,Lin Wen,Yi-Yang Luo,Wen-Juan Hu,Hui-Wen Ren,Ye Lv,Cong Zhang,Ping Gao,Li-Na Xuan,Guan-Yu Wang,Cheng-Jie Li,Zhi-Xin Xiang,Zhi-Lin Luan 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.12

Objective Schizophrenia is a complex and devastating psychiatric disorder with a strong genetic background. However, much uncertainty still exists about the role of genetic susceptibility in the pathophysiology of schizophrenia. TEA domain transcription factor 1 (TEAD1) is a transcription factor associated with neurodevelopment and has modulating effects on various nervous system diseases. In the current study, we performed a case–control association study in a Northeast Chinese Han population to explore the characteristics of pathogenic <i>TEAD1</i> polymorphisms and potential association with schizophrenia.Methods We recruited a total of 721 schizophrenia patients and 1,195 healthy controls in this study. The 9 single nucleotide polymorphisms (SNPs) in the gene region of <i>TEAD1</i> were selected and genotyped.Results The genetic association analyses showed that five SNPs (rs12289262, rs6485989, rs4415740, rs7113256, and rs1866709) were significantly different between schizophrenia patients and healthy controls in allele or/and genotype frequencies. After Bonferroni correction, the association of three SNPs (rs4415740, rs7113256, and rs1866709) with schizophrenia were still evident. Haplotype analysis revealed that two strong linkage disequilibrium blocks (rs6485989-rs4415740-rs7113256 and rs16911710-rs12364619-rs1866709) were globally associated with schizophrenia. Four haplotypes (C-C-C and T-T-T, rs6485989-rs4415740-rs7113256; G-T-A and G-T-G, rs16911710-rs12364619-rs1866709) were significantly different between schizophrenia patients and healthy controls.Conclusion The current findings indicated that the human <i>TEAD1</i> gene has a genetic association with schizophrenia in the Chinese Han population and may act as a susceptibility gene for schizophrenia.

Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Yang Sheng,Xie JiaJun,Pan ZhiJie,Guan HongMei,Tu YueSheng,Ye YuanJian,Huang ShouBin,Fu ShiQiang,Li KangXian,Huang ZhiWei,Li XiaoQi,Shi ZhanJun,Li Le,Zhang Yang 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

The meniscus is vital for maintaining knee homeostasis and function. Meniscal calcification is one of the earliest radiological indicators of knee osteoarthritis (KOA), and meniscal calcification is associated with alterations in biomechanical properties. Meniscal calcification originates from a biochemical process similar to vascular calcification. Advanced glycation end products (AGEs) and their receptors (RAGEs) reportedly play critical roles in vascular calcification. Herein, we investigated whether targeting AGE-RAGE is a potential treatment for meniscal calcification. In our study, we demonstrated that AGE-RAGE promotes the osteogenesis of meniscal cells and exacerbates meniscal calcification. Mechanistically, AGE-RAGE activates mTOR and simultaneously promotes ATF4 accumulation, thereby facilitating the ATF4-mTOR positive feedback loop that enhances the osteogenic capacity of meniscal cells. In this regard, mTOR inhibits ATF4 degradation by reducing its ubiquitination, while ATF4 activates mTOR by increasing arginine uptake. Our findings substantiate the unique role of AGE-RAGE in the meniscus and reveal the role of the ATF4-mTOR positive feedback loop during the osteogenesis of meniscal cells; these results provide potential therapeutic targets for KOA.

Study on the Solid Phase Extraction of Hg(II)-SBDTR Chelate with C₁₈ Disks and Its Application to the Determination of Mercury in Tobacco and Tobacco Additive

Yang, Guan-Gyu,Xia, Zhen-Yuang,Wu, Yu-Ping,Sun, Han-Dong,Yin, Jia-Yuan Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.4

A sensitive, selective and rapid method has been developed for the determination of mercury based on the rapid reaction of mercury(II) with p-sulfobenzylidenethiorhodanine (SBDTR) and the solid phase extraction of the colored chelate with $C_{18}$ disks. In the presence of pH 3.5 sodium acetate-acetic acid buffer solution and Emulsifier-OP medium, SBDTR reacts with mercury(II) to form a red chelate of a molar ratio 1 : 2 (mercury to SBDTR). This chelate was prconcentrated by solid phase extraction with $C_{18}$ disks. An enrichment factor of 50 was achieved. The molar absorptivity of the chelate is $1.28{\times}10^5 L{\cdot}mol^{-1}{\cdot}cm^{-1}$ at 545 nm in measured solution. Beer's law is obeyed in the range of 0.01-3 ${\mu}$g/mL. The relative standard deviation for eleven replicates sample of 0.01 ${\mu}$g/mL is 1.65%. This method was applied to the determination of mercury in tobacco and tobacco additive with good results.

Models and Experiments for the Main Topologies of MRC-WPT Systems

Yang, Mingbo,Wang, Peng,Guan, Yanzhi,Yang, Zhenfeng The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.6

Models and experiments for magnetic resonance coupling wireless power transmission (MRC-WPT) topologies such as the chain topology and branch topology are studied in this paper. Coupling mode theory based energy resonance models are built for the two topologies. Complete energy resonance models including input items, loss coefficients, and coupling coefficients are built for the two topologies. The storage and the oscillation model of the resonant energy are built in the time domain. The effect of the excitation item, loss item, and coupling coefficients on MRC systems are provided in detail. By solving the energy oscillation time domain model, distance enhancing models are established for the chain topology, and energy relocating models are established for the branch topology. Under the assumption that there are no couplings between every other coil or between loads, the maximum transmission capacity conditions are found for the chain topology, and energy distribution models are established for the branch topology. A MRC-WPT experiment was carried out for the verification of the above model. The maximum transmission distance enhancement condition for the chain topology, and the energy allocation model for the branch topology were verified by experiments.

Mitochondrial citrate accumulation drives alveolar epithelial cell necroptosis in lipopolysaccharide-induced acute lung injury

Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.

Experimental Evaluation of Precast Concrete Beam-Column Connections with High-strength Steel Rebars

Dongzhi Guan,Zhengxing Guo,Cheng Jiang,Sen Yang,Hui Yang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.1

A novel precast concrete beam-column connection partially reinforced with high-strength steel rebars of a yield strength greater than 630 MPa was introduced to avoid reinforcement obstruction in connection zones. Reversed cyclic loadings were applied to fullscale specimens to evaluate their seismic performance. For the first precast specimen, high-strength steel rebars acted as bottom longitudinal bars of the beams. For the second precast specimen, high-strength steel rebars were used as both bottom longitudinal bars and embedded bars that were anchored into the joint. The results show that the new precast connection exhibits a satisfactory seismic resistance. The anchored embedded bars are not necessary in the new system for a limited improvement in the structural performance. The mechanical equilibrium of the rectangular stress block method can be applied to estimate the strength of the new connection using the design philosophy of strong column-weak beam.

Models and Experiments for the Main Topologies of MRC-WPT Systems

Mingbo Yang,Peng Wang,Yanzhi Guan,Zhenfeng Yang 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.6

Models and experiments for magnetic resonance coupling wireless power transmission (MRC-WPT) topologies such as the chain topology and branch topology are studied in this paper. Coupling mode theory based energy resonance models are built for the two topologies. Complete energy resonance models including input items, loss coefficients, and coupling coefficients are built for the two topologies. The storage and the oscillation model of the resonant energy are built in the time domain. The effect of the excitation item, loss item, and coupling coefficients on MRC systems are provided in detail. By solving the energy oscillation time domain model, distance enhancing models are established for the chain topology, and energy relocating models are established for the branch topology. Under the assumption that there are no couplings between every other coil or between loads, the maximum transmission capacity conditions are found for the chain topology, and energy distribution models are established for the branch topology. A MRC-WPT experiment was carried out for the verification of the above model. The maximum transmission distance enhancement condition for the chain topology, and the energy allocation model for the branch topology were verified by experiments.