Model Free Deadbeat Predictive Speed Control of Surface-Mounted Permanent Magnet Synchronous Motor Drive system

Yanan Zhou,Hongmei Li,Hengguo Zhang,Jingkui Mao,Jiandong Huang 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.1

In the predictive controlled surface-mounted permanent magnet synchronous motor (SMPMSM) drive system, parametric uncertainties and external disturbances lead to model mismatch and eventually causing the performance degradation. Consequently, this paper proposed a model free deadbeat predictive speed controller (MFDPSC) based on the ultra-local model and deadbeat predictive control. The ultra-local model is established through the input and output variable of the speed loop within parametric uncertainties and external disturbances, then it is used as the predictive model for the design of MFDPSC. The proposed method avoids the requirement of the knowledge of the SMPMSM drive system, and therefore ensures the system robustness. At last, the simulation and experiment are implemented to verify the speed tracking performance of the proposed method, and the results show that the proposed method is robust to parametric uncertainties and external disturbances.

Mechanical properties and bearing capacity of CFRP confined steel reinforced recycled concrete columns under axial compression loading

Hui Ma,Yanan Wu,Cheng Huang,Yanli Zhao 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.79 No.4

To study the axial compression behavior of carbon fiber reinforced plastics (CFRP) confined steel reinforced recycled concrete (CSRRC) columns, 11 specimens of CSRRC columns were manufactured and tested under axial compression loading. The design variables in the experiments included the replacement percentage of recycled coarse aggregate (RCA), layers of CFRP, strength of recycled aggregate concrete (RAC), profile steel ratio and slenderness ratio. Subsequently, the failure process and modes, load-displacement curves, stress-strain curves, transverse deformation coefficient and stiffness degradation of the specimens were obtained and analyzed in detail. The experimental results showed that the profile steel yielded before the steel rebars in the columns, then the RAC was crushed, and finally the CFRP broke under axial compression loading. The axial bearing capacity of CSRRC columns decreased with the increase of replacement percentage of RCA and slenderness ratio, respectively. However, the CFRP can give full play to its high-strength confinement performance and effectively improve the axial bearing capacity and deformability of columns. Moreover, the profile steel ratio and strength of RAC have significant effects on the initial stiffness of CSRRC columns, and the stiffness degradation rate of columns decreases with the increase of these parameters. Overall, the CSRRC columns exhibit high axial bearing capacity and good ductility deformation ability. Based on ACI 440.2R-08, the modified formula on the nominal axial bearing capacity of CSRRC columns was proposed in this study. The accuracy on the modified formulae was evaluated by the comparison between the calculated values and test values.

Morphology characterization of micro-gap oil film of tilting pad hydrostatic bearing under extreme working conditions

Xiaodong Yu,Yanan Feng,Yan Zhao,Dianbin Huang,Jianhua Jiao 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.12

Taking the hydrostatic support of the tiltable oil pad double rectangular cavity as the object, the fluid-thermal-solid coupling deformation analysis of the friction pair is carried out, and the three-dimensional morphology of the micro-gap oil film is obtained. An oil film thickness test device is built to verify the rationality of the numerical simulation method. It is found that the elastic deformations of rotational workbench and base under extreme loads are both concave deformations, the thermal deformations are both convex deformations, and the maximum thermal deformation is mainly concentrated on the outer oil sealing edge. The deformation of the downstream side oil sealing edge is higher than that of the reverse side oil sealing edge. The minimum oil film thickness is the minimum distance between the downstream side oil sealing edge and the lower surface of the hydrostatic guideway of the rotational workbench, where tribological failure is prone to occur.

The effect of the HRB linker of Newcastle disease virus fusion protein on the fusogenic activity

Liu Yaqing,Liu Ying,Huang Yanan,Wen Hongling,Zhao Li,Song Yanyan,Wang Zhiyu 한국미생물학회 2021 The journal of microbiology Vol.59 No.5

Newcastle disease, designated a class A disease of poultry by the Office international des epizooties (OIE), is an acute infection caused by Newcastle disease virus (NDV). The merging of the envelope of NDV with the membrane of a target host cell is the key step in the infection pathway, which is driven by the concerted action of two glycoproteins: haemagglutinin- neuraminidase (HN) and fusion (F) protein. When the HN protein binds to the host cell surface receptor, the F protein is activated to mediate fusion. The three-dimensional structure of the F protein has been reported to have low electron density between the DIII domain and the HRB domain, and this electron-poor region is defined as the HRB linker. To clarify the contributing role of the HRB linker in the NDV F protein-mediated fusion process, 6 single amino acid mutants were obtained by site-directed mutagenesis of the HRB linker. The expression of the mutants and their abilities to mediate fusion were analysed, and the key amino acids in the HRB linker were identified as L436, E439, I450, and S453, as they can modulate the fusion ability or expression of the active form to a certain extent. The data shed light on the crucial role of the F protein HRB linker in the acquisition of a normal fusogenic phenotype.

Proteomic Comparison between Salmonella Typhimurium and Salmonella Typhi

Yue Wang,Kuan-Yeh Huang,Yanan Huo 한국미생물학회 2014 The journal of microbiology Vol.52 No.1

The genus Salmonella contains more than 2500 serovars. While most cause the self-limiting gastroenteritis, a few serovars can elicit typhoid fever, a severe systemic infection. S. enterica subsp. enterica serovar Typhimurium and S. Typhi are the representatives of the gastroenteritis and typhoid fever types of Salmonella. In this study, we adopted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) technology to quantitatively compare the proteomes of the two serovars. We found several proteins with serovar-specific expression, which could be developed as new biomarkers for clinical serotype diagnosis. We found that flagella and chemotaxis genes were down-regulated in S. Typhi in comparison with S. Typhimurium. We attributed this observation to the fact that the smooth cellular structure of S. Typhi may better fit its systemic lifestyle. Instead of known virulence factors that were located within Salmonella Pathogenecity Islands, a number of core genes, which were involved in metabolism and transport of carbohydrates and amino acids, showed differential expression between the two serovars. Further studies on the roles of these differentially-expressed genes in the pathogenesis should be undertaken.

Magnetic properties and enhanced thermoelectric performance in Cu-doped Ca3Co2O6 single crystals

Jiyue Song,Bangchuan Zhao,Yanan Huang,Yanfeng Qin,Wenhai Song,Yuping Sun 한국물리학회 2017 Current Applied Physics Vol.17 No.5

The effect of Cu doping on the structural, magnetic, electrical and thermal transport properties of Ca3Co2- xCuxO6 single crystals has been investigated systematically. Based on the analysis of the structural parameter and x-ray photoelectron spectroscopy spectra, the valence state of Cu is considered to be þ2. All samples undergo a long-range spin density wave (SDW) transition at TN and a glass-like magnetic transition at Tf with decreasing temperature. Both TN and Tf decrease monotonously with increasing Cudoping content. A series of magnetization steps can be observed in the M(H) curve of Ca3Co2O6, and the magnetization steps are sensitive to the sample cooling magnetic field and Cu doping content x. With the increase of x, the resistivity along c-axis decreases while the thermopower increases. As a result, the figure of merit (ZT) increases considerably and the room-temperature ZT value of the sample with x ¼ 0.6 is nearly 60 times larger than that of the un-doped crystal.

Investigating the Characteristics of Wet Shotcrete Pipeline Transportation

Lianjun Chen,Jiahao Sun,Xiaohan Guo,Nan Guo,Yanan Huang,Guoming Liu 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.12

In order to study the resistance characteristics of wet shotcrete pipeline transportation, a small pipe type rheology and resistance test device was developed. The device is used for straight pipe resistance test, pump stop test and dyed concrete test were carried out on concrete with different characteristics. The fluidity changes of concrete with different characteristics were obtained through experiments. The results show that the slump, pressure bleeding rate, water-cement ratio were all negatively related to pipeline transportation resistance. The influence of the three factors on resistance was in order: pressure bleeding rate, water-cement ratio, slump. The dyed concrete test confirmed the principle of the plunger flow in the pipeline when concrete is pumped. The thickness of the lubricating layer increased with the increase of the water-cement ratio. The concrete pipeline transportation resistance increased with the increase of the standing time. The fitting curve was obtained through the correlation analysis between resistance and lubricating layer thickness, and the two were negatively correlated. This provides a new research idea for the study of drag reduction measures for concrete pipeline transportation.

LincR-PPP2R5C Promotes Th2 Cell Differentiation Through PPP2R5C/PP2A by Forming an RNA–DNA Triplex in Allergic Asthma

Ji Ningfei,Chen Zhongqi,Wang Zhengxia,Sun Wei,Yuan Qi,Zhang Xijie,Jia Xinyu,Wu Jingjing,Jiang Jingxian,Song Meijuan,Xu Tingting,Liu Yanan,Ma Qiyun,Sun Zhixiao,Bao Yanmin,Zhang Mingshun,Huang Mao 대한천식알레르기학회 2024 Allergy, Asthma & Immunology Research Vol.16 No.1

Purpose: The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase 2 regulatory subunit B' gamma (PPP2R5C), in Th2 differentiation in a mouse model of asthma. Methods: LincR-PPP2R5C from RNA-seq data of CD4+ T cells of asthma-like mice were validated and confirmed by quantitative reverse transcription polymerase chain reaction, northern blotting, nuclear and cytoplasmic separation, and fluorescence in situ hybridization (FISH). Lentiviruses encoding LincR-PPP2R5C or shRNA were used to overexpress or silence LincR-PPP2R5C in CD4+ T cells. The interactions between LincR-PPP2R5C and PPP2R5C were explored with western blotting, chromatin isolation by RNA purification assay, and fluorescence resonance energy transfer. An ovalbumin-induced acute asthma model in knockout (KO) mice (LincR-PPP2R5C KO, CD4 conditional LincR-PPP2R5C KO) was established to explore the roles of LincR-PPP2R5C in Th2 differentiation. Results: LncR-PPP2R5C was significantly higher in CD4+ T cells from asthmatic mice ex vivo and Th2 cells in vitro. The lentivirus encoding LincR-PPP2R5C suppressed Th1 differentiation; in contrast, the short hairpin RNA (shRNA) lentivirus decreased LincR-PPP2R5C and Th2 differentiation. Mechanistically, LincR-PPP2R5C deficiency suppressed the phosphatase activity of the protein phosphatase 2A (PP2A) holocomplex, resulting in a decline in Th2 differentiation. The formation of an RNA-DNA triplex between LincR-PPP2R5C and the PPP2R5C promoter enhanced PPP2R5C expression and activated PP2A. LincR-PPP2R5C KO and CD4 conditional KO decreased Th2 differentiation, airway hyperresponsiveness and inflammatory responses. Conclusions: LincR-PPP2R5C regulated PPP2R5C expression and PP2A activity by forming an RNA-DNA triplex with the PPP2R5C promoter, leading to Th2 polarization in a mouse model of acute asthma. Our data presented the first definitive evidence of lncRNAs in the regulation of Th2 cells in asthma.