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Patent Ductus Arteriosus and Pulmonary Valve Stenosis in A Patient with 18p Deletion Syndrome
Chun-Hong Xie,Jian-Bin Yang,Fang-Qi Gong,Zheng-Yan Zhao 연세대학교의과대학 2008 Yonsei medical journal Vol.49 No.3
We report on a patient with a partial deletion on the short arm of chromosome 18 (del 18p), who presented with dysmorphic features and delayed developmental milestones as well as with a patent ductus arteriosus (PDA) and pulmonary valve stenosis (PS). Several forms of congenital heart disease (CHD) are found in about 10% of patients with del (18p), but coexisting PDA and PS have not been reported. Del (18p) must be considered in patients with characteristic phenotypic abnormalities and congenital heart disease, including a combination of PDA and PS.
Cheng Wang,Gang-Lin Yan,Shao-Wu Lü,Chun-Hong Sui,Yang Zhao,Ya-Wei Xu,Gang Zhao,Jun-jie Xu,Ping-Sheng Gong,Gui-Min Luo,Ying Mu 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1
Glutathione peroxidase (GPX) is one of the important members of the antioxidant enzyme family. It can catalyze the reduction of hydroperoxides with glutathione to protect cells against oxidative damage. Single-chain variable fragment (scFv) can be converted into seleniumcontaining single-chain variable fragment (Se-scFv) by chemical modification of the hydroxyl groups in scFv, thus Se-scFv possesses GPX activity and becomes a prodrug. To improve the expression of scFv and simplify its purification steps, Single-protein production (SPP) system was used to express scFv and chemical modification was used to synthesize Se-scFv. Therefore, we must construct a new scFv-WCD1-lessACA gene, which can express its mRNA not containing any ACA sequences and express its amino acid sequence of target protein (scFv) being same to scFv-WCD1. In this way, the scFv-WCD1-lessACA can be only expressed in SPP system and no other background proteins in the cells could be expressed. The expression results showed that high level of scFv-WCD1-lessACA synthesis was at least sustained for 96 h in the virtual absence of background protein synthesis. Then, selenocysteine (Sec) was incorporated into the scFv-WCD1-lessACA by chemical modification and resulted in Se-scFv-WCD1-lessACA. The enzymatic characteristics of Se-scFv-WCD1-lessACA were determined. GPX activity was 2,563 U/μmol,its binding constant for GSH was 0.687 ×105/mol. Moreover,Se-scFv-WCD1-lessACA was confirmed to have a strong antioxidant ability to protect mitochondria against oxidative damage induced by Vc/Fe2+ (mitochondrial damage model),suggesting that Se-scFv-WCD1-lessACA has potential application for protection of mitochondrial damage induced by reactive oxygen species (ROS).
( Ruo Peng Cai ),( Yan Long Jiang ),( Wei Yang ),( Wen Tao Yang ),( Shao Hua Shi ),( Chun Wei Shi ),( Jing Tao Hu ),( Wei Gu ),( Li Ping Ye ),( Fang Yu Zhou ),( Qing Long Gong ),( Wen Yu Han ),( Guil 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2
Recently, poly-γ-glutamic acid synthetase A (pgsA) has been applied to display exogenous proteins on the surface of Lactobacillus casei or Lactococcus lactis, which results in a surfacedisplayed component of bacteria. However, the ability of carrying genes encoded by plasmids and the expression efficiency of recombinant bacteria can be somewhat affected by the longer gene length of pgsA (1,143 bp); therefore, a truncated gene, pgsA, was generated based on the characteristics of pgsA by computational analysis. Using murine IL-10 as an exogenous gene, recombinant Lactobacillus plantarum was constructed and the capacity of the surface-displayed protein and functional differences between exogenous proteins expressed by these strains were evaluated. Surface expression of IL-10 on both recombinant bacteria with anchorins and the higher expression levels in L. plantarum-pgsA’-IL-10 were confirmed by western blot assay. Most importantly, up-regulation of IL-1β, IL-6, TNF-α, IFN-γ, and the nuclear transcription factor NF-κB p65 in RAW264.7 cells after stimulation with Poly(I:C) or LPS was exacerbated after co-culture with L. plantarum-pgsA. By contrast, IL-10 expressed by these recombinant strains could reduce these factors, and the expression of these factors was associated with recombinant strains that expressed anchorin (especially in L. plantarum-pgsA’-IL-10) and was significantly lower compared with the anchorin-free strains. These findings indicated that exogenous proteins could be successfully displayed on the surface of L. plantarum by pgsA or pgsA’, and the expression of recombinant bacteria with pgsA’ was superior compared with bacteria with pgsA.
Yuping Li,Li-Hua Yao,Guan-Jie Wu,Xiao-Fang Pi,Yan-Chun Gong,Ruo-Shong Ye,Chen-Xi Wang 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6
Novel small-molecule polysaccharide fractions,named POP II and POP III, were purified from Portulacaoleracea L. with average Mw values of 9.25 and 8.03 kDa,respectively. Monosaccharide analysis revealed that POP IIwas composed of Rha, Ara, Man, Glc, and Gal with molarratios of 1: 1.42: 0.44: 0.88: 1.59. POP III was composedof Rha, Ara, Glc, and Gal with molar ratios of 1: 1.16:0.23: 0.59. The antioxidant activities of the fractions wereevaluated using cell-free and cell-mediated radical generatingsystems. POPII and POP III possessed strong antioxidantactivities in both systems. The 2 novel polysaccharidefractions extracted from P. oleracea L. can be developed asnatural antioxidants for treatment of free radical-relateddiseases.
An Improved Method of Maximum Power Point Tracking Strategy for Wind Power Conversion System
CHEN Ran,CHEN Jie,CHEN Jia-wei,CHEN Zhi-hui,GONG Chun-ying,YAN Yang-guang 전력전자학회 2011 ICPE(ISPE)논문집 Vol.2011 No.5
The turbine speed loop adjustment is the key link of the designing of the controller for the fixed pitch variable speed wind energy conversion systems (WECS). Because of the strong nonlinear, big inertia and mechanical damping characteristics, it’s difficult to designing the controller of the wind power systems. In this paper, a small signal model is present, and based on the analyzing; a wind turbine speed loop regulator is designed. Then, a method of tracking the peak power conversion system is proposed, which is independent of the turbine parameters and air density. At last, simulation system is built, and the results of the simulation experiments show that, the performance of the controller algorithm meet the requirements of the MPPT without wind measurement.