LPL gene Pvu II polymorphism and hypertriglycer-idemia: a meta-analysis involving 1,640 subjects

( Yan-yan Li ),( Yan-hong Zhou ),( Ge Gong ),( Hong-yu Geng ),( Xin-xing Yang ),( Xiang-ming Wang ),( Chuan-wei Zhou ),( Jian Xu ),( Yun Qian ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.6

Background/Aims: Although lipoprotein lipase (LPL) gene Pvu II polymorphism has been associated with an increased risk of hypertriglyceridemia (HT), there is no clear consensus within the scientific community. Methods: A meta-analysis of 1,640 subjects from six individual studies was conducted to better elucidate the potential relationship between the LPL gene Pvu II polymorphism and HT within the Chinese population. Pooled odds ratios (ORs) and their corresponding 95% confidence intervals (CIs) were evaluated by using fixed effect models. Results: Our analysis indicated a significant association between LPL gene Pvu II polymorphism and HT within the Chinese population under allelic (OR, 1.550; 95% CI, 1.320 to 1.830; p = 1.158 × 10^-7), recessive (OR, 0.540; 95% CI, 0.390 to 0.750; p = 0.0002), dominant (OR, 1.889; 95% CI, 1.501 to 2.377; p = 5.960 × 10^-8), homozygous (OR, 2.167; 95% CI, 1.531 to 3.067; p = 1.242 × 10^-5), heterozygous (OR, 1.810; 95% CI, 1.419 to 2.309; p = 1.842 × 10^-6), and additive genetic models (OR, 1.553; 95% CI, 1.320 to 1.828; p = 1.158 × 10^-7). Conclusions: Because LPL gene Pvu II restriction fragment length polymorphism polymorphism was associated with an elevated risk of HT, the P+ allele carriers of the LPL gene might be predisposed to HT.

公司决策权力分配路径的转向 : 法定与意定之间

Wang Yan chuan(王延川) 충북대학교 법학연구소 2014 法學硏究 Vol.25 No.1

The idea that any company organization could not have unlimited decision-making power is the basic concept of company law. This concept requires the power differentiation of the board and shareholder's meeting. And this kind of structure must be regulated by the law. Whether it is stockholders-centerism or board-centerism, the traditional ways of company's decision-making power allocation mostly base on the ownership theory, and maximizing the interests of shareholders as the ultimate goal. This kind of idea should be reviewed. The company is an assembly of all the stakeholders, but shareholder is only one of them. To be fair, shareholder could not be company decision-makers, but based upon the balance of interests, the shareholder can have exceptional minimum supervision. Board of directors as a specialized management organization, as a representative of all the stakeholders, its decision-making authority comes from legal regulations. This kind of power comes from the original provision rather than non-authorized provision. For the allocation of inner decision-making authority, there are some principles should be held on: a) decision-making authority belongs to the board; b) decision-making authority of the shareholder is minimum; c) Power specificity principle and company classify principle. Under the concept of the ownership interest of shareholders, shareholders have excessive decision-making authority, which is unfavorable for improving the efficiency of company management. Decision-making authority will become rigid if it has not been differentiated. This pattern should be improved by legislation and revision.

STUDIES ON SIDESTREAM BIOLOGICAL PHOSPHOROUS REMOVAL PROCESS BY DOSING FERMENTATION LIQUOR

Yang, Zao-yan,Han, Wei,Wang, Le-chuan,Liu, Yan-hua 동아대학교 환경문제연구소 1990 硏究報告 Vol.13 No.3

A sidesteam bio-P removal laboratory scale test unit was carried out to study the possibility of dosing the acid fermentation liquor of primary sludge, excess sludge, mixture of digested sludge with sewage, raw wastewater as substrates for P release into anaerobic P stripper. The results show that the fermentation liquor which contain readily biodegradable substance could be used for bio-P removal. When the retention time in anaerobic P stripper were decreased to 2-6 hours, the P release was stills satisfied. It is possible to keep a higher readily biodegradable COD concentration' in anaerobic stripper because P release is carried out, in more concentrated form, in a small sidestream, when the liquor were added into the stripper. Therefore, not only NO^(-)_(x)-N trouble could be redused, but also P release accelerated.

Roles of mTOR and p-mTOR in Gastrointestinal Stromal Tumors

Li, Jun-Chuan,Zhu, Hong-Yu,Chen, Ting-Xuan,Zou, Lan-Ying,Wang, Xiao-Yan,Zhao, Hui-Chuan,Xu, Jun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Objective: This study aimed to examine the relationship between expression of mammal target of rapamycin (mTOR) and phosphorylation of mTOR (p-mTOR) protein in the PI3K/Akt/mTOR signaling pathways in gastrointestinal stromal tumors and relatiuonships with clinical factors. Methods: Immunohistochemistry was used to detect the expression of the associated proteins mTOR, p-mTOR, and phosphorylation of the tumor suppressor genes PTEN, P27, VEGF, and EGFR in 40 cases of gastrointestinal stromal tumors, with division into a very low and low risk group as well as a moderate and high risk group. Results: The positive rate of mTOR and p-mTOR was significantly increased in the moderate and high risk group compared with the very low and low risk group. The difference was statistically significant (P<0.05). When grouped according to size, the positive mTOR expression rate exhibited a statistical difference (P<0.05), which was significantly increased in the group of tumors larger than 5 cm. The difference in the positive mTOR and p-mTOR expression rate exhibit no statistical significance among the PTEN, P27, VEGF, and EGFR expression subgroups (P>0.05). Conclusion: The different expressions of mTOR and p-mTOR in the signal transduction pathway of gastrointestinal stromal tumor in the different degree-of-risk groups suggested that the mTOR and p-mTOR of the signal transduction pathway serve an important function in the occurrence and development of gastrointestinal stromal tumors.

Characterization of Ha29, a Specific Gene for Helicoverpa armigeraSingle-nucleocapsid Nucleopolyhedrovirus

Chuan-Xi Zhang,Zhong-Jian Guo,Shi-Heng An,Dun Wang,Yan-He Liu,V. Shyam Kumar 한국생화학분자생물학회 2005 BMB Reports Vol.38 No.3

Open reading frame 29 (ha29) is a gene specific for Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearSNPV). Sequence analyses showed that the transcription factor Tfb2 motif, bromodomain and Half-A-TPR (HAT) repeat were present at aa 66-82, 4-76, 55-90 of the Ha29 protein respectively. The product of Ha29 was detected in HearSNPV-infected HzAM1 cells at 3 h post-infection. Western blot analysis using a polyclonal antibody produced by immunizing a rabbit with purified GST-Ha29 fusion protein indicates that Ha29 is an early gene. The size of Ha29 product in infected HzAM1 cells was about 25 kDa, which was larger than the presumed size of 20.4 kDa. Tunicamycin treatment of HearSNPV-infected HzAM1 cells suggested that the Ha29 protein is N-glycosylated. Fluorescent confocal laser scanning microscope examination, and Western blot analysis of purified budded virus (BVs), occlusion-derived virus (O DVs), cell nuclear and cytoplasmic fraction, showed that the Ha29 protein was localized in the nucleus. Our results suggested that ha29 of HearSNPV encodes a non-structurally functional protein that may be associated with virus gene transcription in Helicoverpa hosts.

Agent Bidding Strategy of Multiple Round English Auction based on Genetic Network Programming

Chuan Yue,Shingo Mabu,Yan Chen,Yu Wang,Kotaro Hirasawa 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

The auction mechanism is widely used in web-based sites and originally designed for human beings, but it might not be the most efficientonein the future, while, there is ademand of evolutionary computation auction agents adaptable to the dynamic auction environments. In this paper, we have applied Genetic Network Programming(GNP) to auction agents to determine a bid at each time step and developed multiple round English Auction mechanisms based on multi-agent systems. In the simulations, we provide comparisons of the proposed method with existing ones. As a result, it has been found that the proposed method could help agents to evolve their strategies generation by generation to get more goods with less money. Also, GNP has a good performance of helping the agent to find out the suitable strategy under the current situation.

Transcriptional Analysis of 10 Selected Genes in a Model of Penicillin G Induced Persistence of Chlamydophila psittaci in HeLa Cells

( Yan Qun Hu ),( Li Li Chen ),( Chuan Wang ),( Ya Feng Xie ),( Zhi Xi Chen ),( Liang Zhuan Liu ),( Ze Hong Su ),( Yi Mou Wu ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.8

Chlamydophila psittaci is an important intracellular pathogen. Persistent infection is an important state of the host-parasite interaction in this chlamydial infection, which plays a significant role in spreading the organism within animal populations and in causing chronic chlamydiosis and serious sequelae. In this study, a C. psittaci persistent infection cell model was induced by penicillin G, and real-time quantitative PCR was used to study the transcriptional levels of 10 C. psittaci genes (dnaA, dnaK, ftsW, ftsY, grpE, rpsD, incC, omcB, CPSIT_0846, and CPSIT_0042) in acute and penicillin-G-induced persistent infection cultures. Compared with the acute cultures, the penicillin-G-treated cultures showed a reduced chlamydial inclusion size and a significantly decreased number of elementary body particles. Additionally, some enlarged aberrant reticulate body particles were present in the penicillin- G-treated cultures but not the acute ones. The expression levels of genes encoding products for cell division (FtsW, FtsY) and outer membrane protein E encoding gene (CPSIT_0042) were downregulated (p < 0.05) from 6 h post-infection onward in the persistent infection cultures. Also from 6 h post-infection, the expression levels of DnaA, DnaK, IncC, RpsD, GrpE, and CPSIT_0846 were upregulated (p < 0.05); however, the expression level of OmcB in the persistent infection was almost the same as that in the acute infection (p > 0.05). These results provide new insight regarding molecular activities that accompany persistence of C. psittaci, which may play important roles in the pathogenesis of C. psittaci infection.

Comparative Evaluation of Three Purification Methods for the NucleocapsidProtein of Newcastle Disease Virus from Escherichia coli Homogenates

Yan Peng Tan,Tau Chuan Ling,Khatijah Yusoff,Wen Siang Tan,Beng Ti Tey 한국미생물학회 2005 The journal of microbiology Vol.43 No.3

In the present study, the performances of conventional purification methods, packed bed adsorption (PBA), and expanded bed adsorption (EBA) for the purification of the nucleocapsid protein (NP) of Newcastle disease virus (NDV) from Escherichia coli homogenates were evaluated. The conventional methods for the recovery of NP proteins involved multiple steps, such as centrifugation, precipitation, dialysis, and sucrose gradient ultracentrifugation. For the PBA, clarified feedstock was used for column loading, while in EBA, unclarified feedstock was used. Streamline chelating immobilized with Ni2+ ion was used as an affinity ligand for both PBA and EBA. The final protein yield obtained in conventional and PBA methods was 1.26% and 5.56%, respectively. It was emonstrated that EBA achieved the highest final protein yield of 9.6% with a purification factor of 7. Additionally, the total processing time of the EBA process has been shortened by 8 times compared to that of the conventional method.

Two New Triterpenoid Acids from Kadsura coccinea

Yan Song,Qing-Jie Zhao,Yong-Sheng Jin,Chuan-Wei Feng,Hai-Sheng Chen 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.12

An investigation of EtOAc extracts of Kadsura coccinea (Lem.) A. C. Smith, has led to the isolation of two new compounds characterized as 3-hydroxy-12-hydroxyl coccinic acid (1) and 3-hydroxy-neokadsuranic acid A (2). Their structures were established by 1D and 2D NMR techniques and mass spectroscopy. Antiproliferative effects of the isolated compounds were evaluated against four human tumor cell lines (A549, HCT116, HL-60 and HepG2), and it was found that compound 1 exhibited antiproliferative effects with IC50 values ranging from 3.01 to 18.08 μg/mL.

Establishment of Tripterygium wilfordii Hook. f. Hairy Root Culture and Optimization of Its Culture Conditions for the Production of Triptolide and Wilforine

( Chuan Shu Zhu ),( Guo Qeng Miao ),( Jia Guo ),( Yan Bo Huo ),( Xing Zhang ),( Jia Hua Xie ),( Jun Tao Feng ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.6

In order to solve the shortage of natural Tripterygium wilfordii Hook. f. plant resource for the production of the important secondary metabolites triptolide and wilforine, hairy roots were induced from its root calli by Agrobacterium rhizogenes. Induced hairy roots not only could be maintained and grown well in hormone-free half-strength Murashige and Skoog medium but also could produce sufficient amounts of both triptolide and wilforine. Although hairy roots produced approximately 15% less triptolide than adventitious roots and 10% less wilforine than naturally grown roots, they could grow fast and could be a suitable system for producing both secondary metabolites compared with other tissues. Addition of 50 μM methyl jasmonate (MeJA) could slightly affect hairy root growth, but dramatically stimulated the production of both triptolide and wilforine, whereas 50 μM salicylic acid had no apparent effect on hairy root growth with slightly stimulatory effects on the production of both secondary metabolites. Addition of precursor nicotinic acid, isoleucine, or aspartic acid at the concentration of 500 μM had varying effects on hairy root growth, but none of them had stimulatory effects on triptolide production, and only the former two had slightly beneficial effects on wilforine production. The majority of triptolide produced was secreted into the medium, whereas most of the produced wilforine was retained inside of hairy roots. Our studies provide a promising way to produce triptolide and wilforine in T. wilfordii hairy root cultures combined with MeJA treatment.