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Chen Kun,Chen Xi,Lang Chuandong,Yuan Xingshi,Huang Junming,Li Zhi,Xu Mingyou,Wu Kerong,Zhou Chenhe,Li Qidong,Zhu Chen,Liu Lianxin,Shang Xifu 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
The identification of key regulatory factors that control osteoclastogenesis is important. Accumulating evidence indicates that circular RNAs (circRNAs) are discrete functional entities. However, the complexities of circRNA expression as well as the extent of their regulatory functions during osteoclastogenesis have yet to be revealed. Here, based on circular RNA sequencing data, we identified a circular RNA, circFam190a, as a critical regulator of osteoclast differentiation and function. During osteoclastogenesis, circFam190a is significantly upregulated. In vitro, circFam190a enhanced osteoclast formation and function. In vivo, overexpression of circFam190a induced significant bone loss, while knockdown of circFam190a prevented pathological bone loss in an ovariectomized (OVX) mouse osteoporosis model. Mechanistically, our data suggest that circFam90a enhances the binding of AKT1 and HSP90β, promoting AKT1 stability. Altogether, our findings highlight the critical role of circFam190a as a positive regulator of osteoclastogenesis, and targeting circFam190a might be a promising therapeutic strategy for treating pathological bone loss.
Altered mRNA Levels of MOV10, A3G, and IFN-α in Patients with Chronic Hepatitis B
Zhi-Wei Song,Yan-Xiu Ma,Li-Juan Fu,Bao-qing Fu,Xu Teng,Si-Jia Chen,Wei-Zhen Xu,Hong-Xi Gu 한국미생물학회 2014 The journal of microbiology Vol.52 No.6
To explore the relationship of the MOV10, A3G, and IFN-αmRNA levels with chronic hepatitis B virus (HBV) infection,Blood samples from 96 patients with chronic hepatitis B(CHB) and 21 healthy individuals as control were collected. HBV DNA load and aminotransferase in the serum weretested using real time PCR and velocity methods, respectively. The MOV10, A3G, and IFN-α mRNA levels in theperipheral blood mononuclear cells (PBMC) were examinedthrough qRT-PCR. The MOV10, A3G, and IFN-α mRNAlevels in CHB group was significantly lower than those inthe control group (P<0.01, P<0.05, P<0.01, respectively). TheA3G mRNA level in the high-HBV DNA load group waslower than that in the low-HBV DNA load group (P<0.05). However, no statistical difference was found in the MOV10and IFN-α mRNA levels between the two HBV DNA loadgroups. Furthermore, the MOV10 mRNA level showed positivecorrelation with IFN-α in the control group. These resultsindicated that the expression of the innate immune factorsMOV10, A3G, and IFN-α is affected by chronic HBV infection.
Isoprenaline Induces Periostin Expression in Gastric Cancer
Lin Chen,Guo-Xiao Liu,Hong-Qing Xi,Xiao-Yan Sun,Zhi-Jun Geng,Shao-Wei Yang,Yan-Jie Lu,Bo Wei 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.3
Purpose: Periostin mediates critical steps in gastric cancer and is involved in various signaling pathways. However, the roles of periostin in promoting gastric cancer metastasis are not clear. The aim of this study was to investigate the relevance between periostinexpression and gastric cancer progression and the role of stress-related hormones in the regulation of cancer development and progression. Materials and Methods: Normal, cancerous and metastatic gastric tissues were collected from patients diagnosed with advanced gastric cancer. The in vivo expression of periostin was evaluated by in situ hybridization and immunofluorescent staining. Meanwhile,human gastric adenocarcinoma cell lines MKN-45 and BGC-803 were used to detect the in vitro expression of periostin by using quantitative real-time polymerase chain reaction (PCR) and western blotting. Results: Periostin is expressed in the stroma of the primary gastric tumors and metastases, but not in normal gastric tissue. In addition,we observed that periostin is located mainly in pericryptal fibroblasts, but not in the tumor cells, and strongly correlated to the expression of α-smooth muscle actin (SMA). Furthermore, the distribution patterns of periostin were broader as the clinical staging of tumors progressed. We also identified a role of stress-related signaling in promoting cancer development and progression,and found that isoprenaline upregulated expression levels of periostin in gastric cancer cells. Conclusion: These findings suggest that the distribution pattern of periostin was broader as the clinical staging of the tumor progressedand found that isoprenaline upregulated expression levels of periostin in gastric cancer cells.
Toll-like Receptor 5 Agonism Protects Mice from Radiation Pneumonitis and Pulmonary Fibrosis
Wang, Zhi-Dong,Qiao, Yu-Lei,Tian, Xi-Feng,Zhang, Xue-Qing,Zhou, Shi-Xiang,Liu, Hai-Xiang,Chen, Ying Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9
Radiation pneumonitis and pulmonary fibrosis are the main complications with radiotherapy for thoracic neoplasms, directly limiting the efficient dose in clinical application and currently there are few medicines that effectively function as radioprotectants. However, a TLR5 agonist, CBLB502, was confirmed to have protective efficacy against hematopoietic and gastrointestinal radiation syndromes in mice and primates. This study points to a new direction for protection against thoracic radiation-induced pulmonary syndromes and skin injury by CBLB502. We utilized the TUNEL assay, pathological analysis and immunohistochemistry to obtain evidence thatCBLB502 could alleviate the occurrence of radiation pneumonitis and pulmonary fibrosis as well as radiation-induced skin injury. It may thus play a promising role in facilitating clinical radiotherapy of thoracic neoplasms.
Evaluation and Comparison of the Solubility Models for Solute in Monosolvents
Min-jie Zhi,Wan-feng Chen,Yang-bo Xi 한국화학공학회 2024 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.62 No.1
The solubility of Cloxacillin sodium in ethanol, 1-propanol, isopropanol, and acetone solutions was measured at different temperatures. The melting property was also tested by using a differential scanning calorimeter (DSC). Then, the solubility data were fitted using Apelblat equation and λh equation, respectively. The Wilson model and NRTL model were not utilized to correlate the test data, since Cloxacillin sodium will decompose directly after melting. For comparison purposes, the four empirical models, i.e., Apelblat equation, λh equation, Wilson model and NRTL Model, were evaluated by using 1155 solubility curves of 103 solutes tested under different monosolvents and temperatures. The comparison results indicate that the Apelblat equation is superior to the others. Furthermore, a new method (named the calculation method) for determining the Apelblat equation using only three data points was proposed to solve the problem that there may not be enough solute in the determination of solubility. The log-logistic distribution function was used to further capture the trend of the correlation and to make better quantitative comparison between predicted data and the experimental ones for the Apelblat equation determined by different methods (fitting method or calculation method). It is found that the proposed calculation method not only greatly reduces the number of test data points, but also has satisfactory prediction accuracy.
Optimal Objects of Cooperation Selection for Human Activity in Opportunistic Networks
Jia Wu,Zhi-gang Chen,Xi Yi 한국산학기술학회 2014 SmartCR Vol.4 No.2
Randomness and disconnections during transmission in opportunistic networking have some characteristics similar to human activity. Many traditional methods in opportunistic networks, however, have never achieved effective results when applied to human activity. Consequently, this paper establishes and analyzes a structure tree and then, based on count dependability and usability in personal relationships, an optimized cooperation path is selected. Furthermore, the paper presents a new algorithm?the Optimal Cooperation Path Algorithm ?for opportunistic networks. This algorithm solves the problem of how to choose an appropriate path for human activity in opportunistic networks. When tested during simulations, this algorithm achieved good results.
In vitro Removal of Deoxynivalenol and T-2 Toxin by Lactic Acid Bacteria
Zhong-Yi Zou,Zhi-Fei He,Hong-Jun Li,Peng-Fei Han,Xiao Meng,Yu Zhang,Fang Zhou,Ke-Pei Ouyang,Xi-Yue Chen,Jun Tang 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6
Five strains of lactic acid bacteria were tested for their ability to remove deoxynivalenol (DON) and T-2toxin from MRS broth. The ability of Lactobacillus plantarum strain 102 (LP102) was the strongest among 5strains after incubation at 37oC for 72 h. The mode of removal was physical binding, rather than biotransformation. The abilities were not significantly different between when removing single toxin and when removing mixed toxins by viable cells of LP102. DON and T-2 toxin released from LP102 viable cell-toxin complexes were 28.22±1.55 and 35.42±2.02% of total bound toxins respectively after 3times of wash with posphate buffered saline, respectively,those were 4.59±0.86 and 5.59±1.47% after incubation with simulated gastric fluid (SGF) at 37oC for 4 h, and 6.86±0.81 and 9.04±1.13% after incubation with simulated intestinal fluid (SIF) at 37oC for 4 h, respectively.
Zhang Lu,Yang Zhi-gang,Xu Huayan,Yang Meng-xi,Xu Rong,Chen Lin,Sun Ran,Miao Tianyu,Zhao Jichun,Zhou Xiaoyue,Fu Chuan,Guo Yingkun 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.12
Objective: To determine whether T1 mapping could monitor the dynamic changes of injury in myocardial infarction (MI) and be histologically validated. Materials and Methods: In 22 pigs, MI was induced by ligating the left anterior descending artery and they underwent serial cardiovascular magnetic resonance examinations with modified Look-Locker inversion T1 mapping and extracellular volume (ECV) computation in acute (within 24 hours, n = 22), subacute (7 days, n = 13), and chronic (3 months, n = 7) phases of MI. Masson’s trichrome staining was performed for histological ECV calculation. Myocardial native T1 and ECV were obtained by region of interest measurement in infarcted, peri-infarct, and remote myocardium. Results: Native T1 and ECV in peri-infarct myocardium differed from remote myocardium in acute (1181 ± 62 ms vs. 1113 ± 64 ms, p = 0.002; 24 ± 4% vs. 19 ± 4%, p = 0.031) and subacute phases (1264 ± 41 ms vs. 1171 ± 56 ms, p < 0.001; 27 ± 4% vs. 22 ± 2%, p = 0.009) but not in chronic phase (1157 ± 57 ms vs. 1120 ± 54 ms, p = 0.934; 23 ± 2% vs. 20 ± 1%, p = 0.109). From acute to chronic MI, infarcted native T1 peaked in subacute phase (1275 ± 63 ms vs. 1637 ± 123 ms vs. 1471 ± 98 ms, p < 0.001), while ECV progressively increased with time (35 ± 7% vs. 46 ± 6% vs. 52 ± 4%, p < 0.001). Native T1 correlated well with histological findings (R2 = 0.65 to 0.89, all p < 0.001) so did ECV (R2 = 0.73 to 0.94, all p < 0.001). Conclusion: T1 mapping allows the quantitative assessment of injury in MI and the noninvasive monitoring of tissue injury evolution, which correlates well with histological findings.