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      • KCI등재

        Experimental and analytical investigation on RC columns with distributed-steel bar

        Mao Ye,Yinpei Pi,Min Ren 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.47 No.6

        Distributed-Steel Bar Reinforced Concrete (DSBRC) columns, a new and innovative construction technique for composite steel and concrete material which can alleviate the difficulty in the arrangement of the stirrup in the column, were studied experimentally and analytically in this paper. In addition, an ordinary steel Reinforced Concrete (SRC) column was also tested for comparison purpose. The specimens were subjected to quasi-static load reversals to model the earthquake effect. The experimental results including the hysteresis curve, resistance recession, skeleton curves and ductility ratio of columns were obtained, which showed well resistant-seismic behavior for DSBRC column. Meanwhile a numerical three-dimensional nonlinear finite-element (FE) analysis on its mechanical behavior was also carried out. The numerically analyzed results were then compared to the experimental results for validation. The parametric studies and investigation about the effects of several critical factors on the seismic behavior of the DSBRC column were also conducted, which include axial compression ratios, steel ratio, concrete strength and yield strength of steel bar.

      • Prostate Cancer Risk in Relation to a Single Nucleotide Polymorphism in the Insulin-like Growth Factor-binding Protein-3 (IGFBP3) Gene: a Meta-analysis

        Mao, Ye-Qing,Xu, Xin,Lin, Yi-Wei,Chen, Hong,Hu, Zheng-Hui,Xu, Xiang-Lai,Zhu, Yi,Wu, Jian,Zheng, Xiang-Yi,Qin, Jie,Xie, Li-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

        Insulin-like growth factor-binding protein-3 (IGFBP3) has been identified as a putative tumor suppressor with multifunctional roles in the IGF axis. Recently, there have been a growing body of studies investigating the relation between the IGFBP3 A-202C polymorphism, circulating IGFBP3 and prostate cancer risk, but their outcomes varied leading to controversy. Hence, it is necessary to perform a meta-analysis covering all eligible studies to shed a light on the association of IGFBP3 A-202C and cancer risk. Finally, we included a total of 11 relevant articles between 2003 and 2010 covering 14 case-control studies including 9,238 cases and 8,741 controls for our analysis. Our results showed that A-202C was a marginal risk factor of prostate cancer (allele contrast: OR=1.08, 95% CI :1.01-1.16; dominant model: OR=1.11, 95% CI :1.01-1.22; heterozygote codominant model: OR=1.11, 95% CI :1.03-1.18; homozygote contrast: OR=1.19, 95% CI :1.03-1.37). Stratification analysis revealed that sample size and control source were two major heterogeneous meta-factors especially in the recessive model (source: Population-based control group :p=0.30,I2=16.7%, Hospital-based control group: p=0.20, I2=30.3%; sample size: Small: p=0.22,I2= 32.8%, Medium: p=0.09,I2=48%, Large p=0.60,I2=0.0%); However, contrary to previous findings, no significance was found in racial subgroups. No significant publication bias was found in our analysis. Considering the robustness of the results and the discrepancy among some studies, there might be some unsolved confounding factors, and further more critical large studies are needed for confirmation.

      • SCIESCOPUS

        Experimental and analytical investigation on RC columns with distributed-steel bar

        Ye, Mao,Pi, Yinpei,Ren, Min Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.47 No.6

        Distributed-Steel Bar Reinforced Concrete (DSBRC) columns, a new and innovative construction technique for composite steel and concrete material which can alleviate the difficulty in the arrangement of the stirrup in the column, were studied experimentally and analytically in this paper. In addition, an ordinary steel Reinforced Concrete (SRC) column was also tested for comparison purpose. The specimens were subjected to quasi-static load reversals to model the earthquake effect. The experimental results including the hysteresis curve, resistance recession, skeleton curves and ductility ratio of columns were obtained, which showed well resistant-seismic behavior for DSBRC column. Meanwhile a numerical three-dimensional nonlinear finite-element (FE) analysis on its mechanical behavior was also carried out. The numerically analyzed results were then compared to the experimental results for validation. The parametric studies and investigation about the effects of several critical factors on the seismic behavior of the DSBRC column were also conducted, which include axial compression ratios, steel ratio, concrete strength and yield strength of steel bar.

      • KCI등재

        An Efficient Fire Detection Method Based on Orientation Feature

        Mao Ye,Tao Li,Feng Pang,Haiyang Wang,Jian Ding 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.5

        This paper proposes a novel method for reliable fire detection. The burning fire usually causes rich moving features in terms of directions, which can offer the best chance to distinguish between the fire region and the non-fire one. Motivated by this observation, we design a novel orientation feature to represent this characteristic. Based on this feature, a method is proposed to detect the fire efficiently. First, fire color is utilized to extract the fire candidate areas from the surveillance video. Then, the direction is obtained by computing the optical flow for each pixel in the candidate area. The directions are discretized to four parts. By counting the percentage of pixels whose moving directions fall into these four parts in a period of time, and combining with the two parameters, i.e., both of the number of frames without the moving directions and the number of consecutive frames in the candidate area, we use these six parameters as the fire orientation feature. In the end, by training a support vector machine (SVM) classifier with the input of our fire orientation feature, the candidate area is judged whether it is a fire. Our main contribution is that we design the novel fire orientation feature. The fea-ture can not only characterize the fire intrinsic dynamic properties accurately but also is very efficient. Compared with the art-of-state methods, the experimental results confirm that our approach signifi-cantly improves the accuracy of fire detection and impressively decreases the false alarm rate. The de-tection speed of our approach is also very competitive with the art-of-state fire detection methods.

      • KCI등재

        Genome-wide analysis of chemosensory protein genes in the small white butterfly Pieris rapae (Lepidoptera: Pieridae)

        Mao-Ye Li,Xiu-Yun Jiang,Xi-Ya Liu,Yuan-Jie Huang,Shi-Guang Li,Su Liu 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.3

        Chemosensory proteins (CSPs) play a crucial role in olfactory recognition in insects. The small white butterfly Pieris rapae—a major pest of Brassicaceae vegetables, which causes enormous economic losses—uses olfaction to locate its host plants. However, the molecular mechanism of olfaction in this species remains unknown. Herein, we performed a genome-wide and transcriptome-wide analysis of CSP genes in P. rapae and identified 21 CSPs (PrapCSP1 to PrapCSP21). Proteins encoded by these genes showed typical characteristics of CSPs—an Nterminal signal peptide and four positionally conserved cysteine residues. BLASTX analysis indicated that most P. rapae CSPs showed high amino acid identity with their respective orthologs in other lepidopterans. Phylogenetic analysis showed that most P. rapae CSPs were well segregated and were clustered into different branches. The 21 genes were located on six genomic scaffolds, and most genes were tandemly arrayed. Quantitative reverse transcription-PCR showed that PrapCSP3, 4, 16 and 21 had the highest expression level in the antennae; PrapCSP7 and PrapCSP18 were mainly expressed in the ovaries, and PrapCSP9 and PrapCSP17 were leg-enriched. PrapCSP11 and PrapCSP20 were found mainly in the heads and testes, respectively. Our findings provide a solid foundation for studying the function of these genes.

      • SCIESCOPUSKCI등재

        Purification and Characterization of an Acid Deoxyribonuclease from the Cultured Mycelia of Cordyceps sinensis

        ( Mao Qing Ye ),( Zheng Hu ),( Ying Fan ),( Ling He ),( Fu Bao Xia ),( Guo Lin Zou ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.4

        A new acid deoxyribonuclease (DNase) was purified from the cultured mycelia of Cordyceps sinensis, and designated CSDNase. CSDNase was purified by (NH₄)₂SO₄ precipitation, Sephacryl S-100 HR gel filtration, weak anion-exchange HPLC, and gel filtration HPLC. The protein was single-chained, with an apparent molecular mass of ca. 34 kDa, as revealed by SDS-PAGE, and an isoelectric point of 7.05, as estimated by isoelectric focusing. CSDNase acted on both double-stranded (ds) and single- stranded (ss) DNA, but preferentially on dsDNA. The optimum pH of CSDNase was pH 5.5 and its optimum temperature 55. The activity of CSDNase was not dependent on divalent cations, but its enzymic activity was inhibited by high concentration of the cation: MgC1₂ above 150 mM, MnCl₂ above 200 mM, ZnCl₂ above 150 mM, CaCl₂ above 200 mM, NaCl above 300 mM, and KCI above 300 mM. CSDNase was found to hydrolyze DNA, and to generate 3-phosphate and 5-OH termini. These results indicate that the nucleolytic properties of CSDNase are essentially the same as those of other well-characterized acid DNases, and that CSDNase is a member of the acid DNase family. To our knowledge, this is the first report of an acid DNase in a fungus.

      • KCI등재

        Identification of six heat shock protein 70 genes in Lasioderma serricorne (Coleoptera: Anobiidae) and their responses to temperature stress

        Li Mao-Ye,Huang Yan,Lei Xiao,Xu Chuan-Tao,Li Bin,Chen De-Xin,Liu Su 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

        Heat shock proteins (HSPs) play essential roles in stress tolerance in insects, such as the cigarette beetle, Lasioderma serricorne, which is an important pest of stored products. However, the function of HSPs in envi ronmental stress response in L. serricorne is poorly understood. In this study, six cDNAs encoding putative HSP70s were identified in L. serricorne (LsHSP70-1 to LsHSP70-6). The LsHSP70 proteins identified have signature motifs of insect HSP70s and exhibit high amino-acid identity with their respective orthologs from other insect species. Phylogenetic analysis showed that these LsHSP70s fall into three major clans: HSP70 in the cytosol (four genes), endoplasmic reticulum (one gene), and mitochondria (one gene). LsHSP70 genes are differentially expressed in different developmental stages. LsHSP70-4 and LsHSP70-5 are mainly expressed at the larval stage, while LsHSP70-3 shows the highest level at the pupal stage. The other genes are ubiquitously expressed. Furthermore, the expression levels of LsHSP70-1 and LsHSP70-4 are significantly upregulated upon exposure to temperatures of both 0 and 15 ◦ C, while LsHSP70-3 is inducible at 15 ◦ C and LsHSP70-6 at 0 ◦ C. In addition, treatment at 0 ◦ C causes significant downregulation of LsHSP70-2 and LsHSP70-3. Exposure to a temperature of 30 ◦ C upregulates LsHSP70-1 expression, while 35 and 40 ◦ C treatments result in significantly enhanced transcription of all the LsHSP70 genes. To the best of the authors’ knowledge, this is the first report on the sequence characteristics, phylogenetic relationships, and expression profiles of HSP70 genes in L. serricorne. The cold- and heat-inducible regulation of LsHSP70s suggests that these genes are related to tolerance of abnormal temperatures.

      • rs12904 Polymorphism in the 3'UTR of EFNA1 is Associated with Colorectal Cancer Susceptibility in a Chinese Population

        Mao, Ying-Ying,Jing, Fang-Yuan,Jin, Ming-Juan,Li, Ying-Jun,Ding, Ye,Guo, Jing,Wang, Fen-Juan,Jiang, Long-Fang,Chen, Kun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

        Accumulated evidence has indicated that Ephrin A1 (EFNA1) is associated with angiogenesis and tumorigenesis in various types of malignancies, including colorectal cancer (CRC). In the current study, we performed an online search using the public microarray database to investigate whether EFNA1 expression might be altered in CRC tissues. We then conducted a case-control study including 306 subjects (102 cases and 204 well-matched controls) in Xiaoshan County to assess any association between genetic polymorphisms in EFNA1 and CRC susceptibility. Searches in the Oncomine expression profiling database revealed EFNA1 to be overexpressed in CRC tissue compared with adjacent normal tissue. The rs12904 G-A variant located in the 3' untranslated region (UTR) of EFNA1 was observed to be associated with CRC susceptibility. Compared with the AA homozygous genotype, those carrying GA genotype had a decreased risk of developing CRC (odds ratio (OR)=0.469, 95% confidence interval (CI): 0.225-0.977, and P=0.043). The association was stronger among smokers and tea drinkers, however, no statistical evidence of interaction between rs12904 polymorphism and smoking or tea drinking on CRC risk was found. Our results suggest that EFNA1 is involved in colorectal tumorigenesis, and rs12904 A>G polymorphism in the 3' UTR of EFNA1 is associated with CRC susceptibility. Larger studies and further mechanistic investigations are warranted to confirm our findings.

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