Cellulase-amyloglucosidase와 효모의 가스생성법에 의한 사료의 에너지가 측정에 관한 연구

김영길,이광목,김욱 東亞大學校生命資源科學大學附設 農業資源硏究所 1993 農業生命資援硏究 Vol.2 No.2

粗飼料와 濃厚飼料를 混合한 飼料의 營養價를 Cellulase와 Amyloglucosidase, 酵母를 利用한 가스 生成法에 의하여 消化率과 TDN을 測定할 수 있는 方法을 確立하고자 本 硏究를 實施한 實驗 結果는 다음과 같다. 1. Cellulose 加水分解 酵素인 Trichoderma viride cellulase의 最適 活性 pH는 4.5-4.8이었으며 pH가 5.0보다 높을 때에는 活性이 急激히 저하하였다. 2. Cellulose 加水分解시 Trichoderma viride celulase의 溫度의 의한 影響은 50℃에서 最大 活性을 나타내었으며, 40℃에서는 活性이 92%정도로 나타났다. 3. Trichoderma viride-aspergillus niger 混合, cellulase 使用時의 가스 生成量은 Trichoderma viride cellulase 使用時 가스 生成量 보다 20-30% 높게 나타났으므로 cellulose 加水分解時 Trichoderma viride-aspergillus niger 混合 cellulase를 使用하는 것이 더욱 效果的일 것으로 思料된다. 4. 포도당(X)과 가스 生成量(Y)과의 關係는 Y=6.82+165X의 回歸式과 相關係數는 0.92로서 高度의 有意性(P<0.01)이 있었다. 5. Trichoderma viride cellulase 使用時 가스 生成量(X)과 乾物 消化率(Y)과의 關係는 Y=29.9+1.13X(r=78**)의 回歸式을 나타내었고, Trichoderma viride-aspergillus niger 混合 vellulase 使用時 가스 生成量(X)과 乾物 消化率(Y)과의 關係는 Y=28.9+0.88X(r=0.82**)의 回歸式을 나타내었다. Trichoderma viride cellulase 使用時 보다 Trichoderma viride-aspergillus niger 혼합 cellulase 사용시 相關係數가 더 높았다. 6. 가스 生成量(X)과 TDN(Y)과의 關係는 Y=35.8+0.585X의 回歸式과 相關係數 0.93으로서 高度의 有意性을 나타내었고, TDN(X)과 가스 生成量(Y)과의 關係는 Y=1.68X-59.6의 回歸式을 나타내었다. The experiment was conducted to establish the gas production method by cellulase, amyloglucosidase and yeast to determine the digestibility and energy value(TDN) of feedstuffs. The results obtained were summarized as follows; 1. The highest activity of Trichoderma viride cellulase was shown at pH 4.8 and the activity of the cellulase decreased rapidly at higher pH than 5.0. 2. The highest activity of Trichoderma virie cellulase was shown at 50℃ and the activity at 40℃ was approximately 92% compared with that at 50℃. 3. The mixed celllulases of Trichoderma viride and Aspergillus nigher produced more gas than Trichoderma viride cellulase when incubated with feedstuffs and were identified to be more effectively used for gas production method. 4. The regression equation of gas production(Y) on glucose production (X) was Y=6.82+165X with significant correlation coefficient of 0.92. 5. The regression equation of DMD(Y) on gas production(X) was Y=29.9+1.13X(r=0.78) with Trichoderma viride cellulase and Y=28.9+0.88X (r=0.82) with both Trichoderma viride and Aspergillus niger cellulase. 6. The regression equation of TDN(Y) on gas production(X) was Y=35.8+0.58X with significant correlation coefficients of 0.93 and in conclusion energy value of feedstuffs can be estimated by gas production method.

CELLULASE - AMYLOGLUCOSIDASE 와 효모의 가스생성법에 의한 사료의 에너지가 측정에 관한 연구

김영길(Y . K . Kim),이광묵(K . M . Lee),김욱(U . Kim) 한국영양사료학회 1993 韓國營養飼料學會誌 Vol.17 No.3

U-드로우 굽힘 마찰시험에 의한 마찰계수 평가

이승욱(S. U. Lee),문지훈(J. H. Mun),홍상휘(S. H. Hong),김정호(J. H. Kim),곽성훈(S. H. Kwak),김영석(Y. S. Kim) 한국기계가공학회 2013 한국기계가공학회 춘추계학술대회 논문집 Vol.2013 No.5

간이 코오넬 의학지수에 의한 부산시내 산업장 근로자들의 건강 상태에 관한 조사

김돈균,김준연,김용준,이채언,이종철,유병호 부산대학교 1972 論文集 Vol.13 No.1

An investigation was conducted by authors from April 1st to August 30th, 1971 on the physical as well as mental complaints of industrial workers, through applying the Modified Cornell Medical Index forwards a total of 4,914 labourers working in Pusan City. The obtained results were as follows : 1. The highest average number of complaints was seen with the age group of 19-23, numbering 10.9 in male and of below 18, numbering 14.9 in female. The largest number of cases with no complaints was seen with those belonging to the age group above 54, rating 42.5% in male and above 54, rating 50.0% in female. The rate of cases giving 10 or more complaints throughout all sections was the highest with the age group below 18 and 19-23, rating 44.9% in male and below 18, rating 65.7% in female. 2. The "no education group" of all, in case of male, gave the largest number of complaints averaging 10.8 and in case of female, "can read Korean group" had the highest average number of complaints, numbering 15.1. The largest number of cases with no complaints was seen with those belonging to the primary school graduates, rating 20.8% in male and "no education group", rating 9.6% in female. Those giving more than 10 complaints covering all sections rated 38.8% or highest with the "no education group" in male and rated 65.9% or highest with the educational level of "high school graduates group" in female. 3. By living standards, the largest average number of complaints was seen with the "middle" class numbering 9.6 in male and also "middle" class, numbering 14.3 in female, "low" class of female demonstrated the highest rate of "no complaint" rating 19.0% while in case of female, "upper" class showed the highest rate, 12.5%. The middle classes of male and female occupied the highest rate (36.6% and 64.7% respectively) of giving 10 or more complaints covering all sections. 4. By religion those believing in Chun-ri-Kyo in male and Catholicism in case of female gave the largest average number of complaints, numbering 13.0 and 15.4 respectively, 21.4% of Confucian group in male and 13.6% of those believing in other religion in female revealed the highest rate of giving no complaints. Those believing Chun-ri-Kyo of male and Catholics of female respectively demonstrated the highest rate (Chun-ri-Kyo : 50.0%, Catholic 70.6%) in giving 10 or more complaints covering all sections. 5. By the number of family size the highest average number of complaints are seen with the family size of only one, numbering 10.4 in male and of severe, numbering 16.1 in female. The largest number of cases with no complaints was seen with those belonging to the family size of 3, rating 20.9% in male and of 2, rating 13.3% in female. The rate of cases gives 10 or more complaints throughout all sections was the highest with the family size of only one, rating 44.8% in male and of also only one, rating 71.7% in female.

연 취급 근로자들의 생화학적 검사에 관한 연구

김준연,이채언,전진호,문덕환,이명철,김병수,김용완 인제대학교 1985 仁濟醫學 Vol.6 No.3

연 취급 근로자 236명과 대학생 120명을 대상으로 연 폭로에 관련되는 생물학적 지표들의 농도를 측정한 결과 PbB, Hb, PbU, ALAU에서 양군간에 유의한 차이를 시현하였으며, 연 근로자에서 PbU 및 CPU가 PbB의 증가에 따라 점증 경향을 보였다. To acquire the fundamental data to control the harmful effect by lead exposure, the authors examined several biological indicators relevant to lead exposure on 236 female lead workers in the electronics industry and 120 controls for one year from August, 1984. The obtained results were as follows; 1.The mean value of PbB, Hb, PbU, CPU and ALAU in lead workers was 24.56± 12.55㎍/100ml,13.53±0.90g/100ml, 58.54±40.00㎍/l, 39.63±26.09㎍/l and 2.33±1.3mg/l, respectively. 2.The mean value of PbB, Hb, PbU, CPU anti ALAU in controls was 16.54± 10.40㎍/100ml, 15.10±1.55g/ml, 30.74±19.05㎍/l, 34.30±26.86㎍/l, and 1.98±1.37mg/l, respectively, and the difference of the mean value of PbB, Hb, PbU and ALAU between lead workers and controls was statistically significant. 3.In lead workers, PbB and PbU, PbU and ALAU, CPU and ALAU showed significant correlation. 4.In lead workers, there showed increasing tendency of PbU and CPU as increasing PbB with statistical significance.

한국인의 CCR5 유전자형은 wild homozygous이나 HIV 감염자의 LTNP 비율은 Caucasian과 유사

김성순,최병선,김은영,고운영,박근용,김태규,신영오,이주실 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Nuclear Design Feasibility of the Soluble Boron Free PWR Core

Kim, J.-C 한국원자력학회 1998 Nuclear Engineering and Technology Vol.30 No.4

제조업 산업장의 작업 공정과 분진 작업장내의 분진 농도에 관한 조사 연구

김병수,김성천,이채언,전진호,김진옥,김준연 인제대학교 1986 仁濟醫學 Vol.7 No.1

부산지역의 35개 제조업 산업장을 대상으로 작업 공정을 파악하고 분진 작업 부서별 총 분진 농도를 산정한 절과 선박건조 및 수선업의 분진 농도가 비교적 높게 시현되었으며, 많은 산업장에서1mg/m3 미만의 분진 농도를 정하였다. In order to contribute to improve working environments and preventive health effects for workers exposed to dust, the authors surveyed the working the working process and measured the total dust concentration in dusty process with the subject of 38 manufacturing industries in Pusan area for 4 months from April to July in 1985. The results were summarized as follows: 1.By the kind of industry, total dust concentration was relatively high in shipbuilding and repairing (2.31-12.83mg/m3) and motor vehicle manufacturing (0.27-6.81mg/m3), and relatively low in preserving and processing of fish, crustacea and similar foods (0.28-0.41mg/m3) and milling of cereals (0.34-0.98mg/m3). 2.By the dusty process, the mean concentration of total dust in major assembling (7.98mg/m3), minor assembling (6.26mg/3), pipe arrangement (5.08mg/m3), decorative (4.64mg/m3), Short(4.47/m3) of shipbuilding and repairing was above the threshold limit value (4mg/m3), and the upper level of total dust concentration range in assembling(6.81mg/m3) of motor vehicle manufacturing and grinding (4.03mg/m3) of stainless products manufacturing exceeded the threshold limit value. 3.The mean concentration of total dust was less than 1mg/m3 in most subjected dusty processes (about 65%) such as scouring, spinning of textile industry, rotary and adhesion of plywood manufacturing industry, and so on.

보건의약관계 국가시험제도 개선에 관한 조사연구 : 치과기공사 국가시험 제도 및 시행관리 개선에 관한 조사연구

김용구,유원식,유진호,김지환,오세윤,김웅철 국립보건연구원 1994 국립보건원보 Vol.31 No.2

Lactobacilli에 의한 Bacillus cereus 의 생장억제에 관한 연구

김현욱,안영태,김용희,장영호 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.3

The growth inhibition effect of Bacillus cereus by lactobacilli has been studied. As L. acidophilus HF 04 or L. acidophilus NCFM was co-incubated with B. cereus in skim milk, the germination and sporulation of B. cereus were inhibited without affecting the growth of L. acidophilus strains. The growth curve of lactobacilli reached stationary phase after IS hours of incubation and the number of B. cereus cells decreased drastically after 10 hours of incubation. The growth of B. cereus was not observed in the skim milk medium lower than pH 4.3. The organic acids produced by lactobacilli were confirmed to inhibit the growth of B. cereus. Acetic acid was more effective growth inhibitor of B. cereus LTDM03 in skim milk medium and thermoduric spores were not germinated after 10 hours of incubation in the skim milk medium adjusted to pH 4.63 by acetic acid.