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젖산균과 비피더스균에 의한 Escherichia coli O157 : H7 과 Salmonella typhimurium 의 생장억제
안영태,신필기,김현욱 한국식품위생안전성학회 1997 한국식품위생안전성학회지 Vol.12 No.3
Lactobacillus acidophilus NCFM, Lactobacillus casei YIT 9018, Bifidobacterium longum 8001, and Bifadobacterium longum 8025 at the level of 10^6 cfu/ml were cultured with 10⁴ cfu/ml of Escherichia colt O157:H7 KSC 109 or Salmonella typhimurium ATCC 14028, in order to verify the effects of lactic acid bacteria and bifidobacteria on the growth of the pathogens. In the mixed culture of lactic acid bacteria with E. colt O157:H7 KSC 109, growth inhibition and atypical microcolonies of E. colt O157:H7 KSC 109 were observed. The pathogens inoculated grew for 5 hours (pH 5.3), by the time L. acidophilus NCFM reached the exponential growth phase, and then the surviving pathogens were decreased to 10¹ cfu/ml after 35 hours. When L. casei YIT 9018 was grown with the pathogens, they grew for 10 hours (pH 4.6), by the time L. casei YIT 9018 reached the end of exponential growth phase, and then the surviving pathogens were decreased drastically. Up to the stationary growth phase of lactic acid bacteria, L. acidophilus NCFM exhibited stronger inhibition against the pathogens than L. casei YIT 9018 did, which might be attributed to its faster growth. Likewise bifidobacteria inhibited the growth of the pathogens. tested, bifidobaceria was weaker in the inhibitory activity than lactic acid bacteria. When Bifidobacterium longum 8001 was cultured with the pathogens, E. colt O157:H7 KSC 109 was gradually inhibited at the stationary growth phase of bifidobacteria, atypical microcolonies were formed on Levine EMB medium after 48 hours, and Salmonella grew up to 10^6 cfu/ml, then was drastically inhibited at the exponential growth phage of Bifxdobacterium longum 8001. But when Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same level of Bifidobacteriuam longum 8025 after 10 hours, then the surviving pathogens were decreased drastically.
젖산균의 혈중 Cholesterol 저하기능에 관한 고찰
안영태,김현욱 한국유가공기술과학회 1999 Journal of Dairy Science and Biotechnology (JMSB) Vol.17 No.2
High serum cholesterol concentration are associated with the development of coronary heart disease, the major cause of death in the world. The consumption of milk products containing some lactobacilli or bifidobacteria are known to have a beneficial effect in lowering the concentration of serum cholesterol in humans, swine, rats. However, due to some of the controversial results it is not possible to draw any definitive conclusion on a hypocholesterolemic effect of fermented milk products. Recently, bile salt deconjugation by lactic acid bacteria has been proposed as the mechanism of hypocholesterolemic effects. Since conjugated bile acids are necessary to emulsify cholesterol in small intestine, bile salt deconjugation by lactic acid bacteria can decrease intestinal absorption of cholesterol. Free bile acids as well as cholesterol are less soluble than the conjugated bile acid at intestinal pH(pH 5.5∼6.5). Therefore, lesser amount of free bile acids and cholesterol are absorbed through the enterohepatic circulation and most of them are easily excreted via feces. Thus, serum cholesterol could be removed from the body's pool. Because L. acidophilus strains tend to exhibit host specificity, the most effective strains as dietary adjuncts for humans to aid serum cholesterol control should theoretically be originated from human intestines. The fermented milk containing lactobacilli having highly bile salt deconjugation activity is presumed to higher hypocholesterolemic effects.
한국 성인의 분과 유제품에서 발견된 젖산균의 복합담즙산염 분해에 관한 연구
안영태,김현욱 한국축산학회 1999 한국축산학회지 Vol.41 No.2
Bile salt deconjugation of one hundred-thirty lactic acid bacterial isolates from human feces, fermented milk products, and probiotic capsules was investigated on MRS media containing oxgall, bile extract(porcine), bile (bovine), TCA(taurocholic acid), TCDCA(taurochenodeoxycholic acid), TDCA(taurodeoxycholic acid), GCA (glycocholic acid), GCDCA(glycochenodeoxycholic acid), and GDCA(glycodeoxycholic acid). Lactobacillus fermentum, L. brnvis, L. plantarum, and Lactococcus lactis ssp. lactis had higher deconjugation activity of bile salts than L. acidophilus strains. When bile salts were deconjugated, halos of precipitate around colonies or opaque granular white colonies appeared on MRS agar containing bile salts. Opaque granular white colonies were formed when TDCA and GDCA were deconjugated. No or weak deconjugation activity was found under aerobic conditions. Fifty-three lactic acid bacterial isolates from human feces and thirteen isolates from fermented milk products or probiotic capsules were found to have high deconjugation activity of bile salts. When deconjugation of bile salts by selected lactic acid bacteria was tested on taurine- and glycine-conjugated bile acids, L. acidophilus FMP 0101 and L. axidophilus HF 0404 deconjugated all the conjugated bile acids tested except for GCDCA and GDCA, and these two strains deconjugated more taurine- than glycine-conjugated bile acid. E. faecium HP 0201 isolated from commercial probiotic capsules had the highest deconjugation activity among the examined bacterial isolates. Optimum pH for the bile salt hydrolase of the two L. acidophilus strains appeared to be pH 5.0-5.4 and the bile salt hydrolase activity of both L. acidophilus strains from human feces was higher inside the cell than in the supernatant without bile extract. When bile extract was added to the media, the enzyme activity of L. acidophilus HF 0401 was high both inside the cell and in the supernatant, but L. acidophilus HF 0404 exhibited higher enzyme activity in the supernatant than inside the cell.
안영태,이호용,이정희,김동현,허철성 한국유산균·프로바이오틱스학회 2013 Current Topic in Lactic Acid Bacteria and Probioti Vol.1 No.1
The anti-colitic effect of lactic acid bacteria mixture (R&B Balance<sup>®</sup>) consisting of Lactobacillus brevis HY7401, Lactobacillus helveticus HY7801 and Bifidobacterium longum HY8004 was investigated in dextran sulfate sodium (DSS)-induced colitic mice. Treatment with R&B Balance<sup>®</sup> reduced severe colitic symptoms, including colon shortening, myeloperoxidase (MPO) activity and malondialdehyde (MDA). The oral administration of R&B Balance<sup>®</sup> significantly reduced the activities of fecal chondroitinase and the fecal genotoxicity. Changes of fecal microflora in mice treated with DSS with or without R&B Balance<sup>®</sup> were assessed by terminal restriction fragment length polymorphism (T-RFLP). Cluster analysis of T-RFLP patterns demonstrated that microflora of DSS-induced colitic mice differed from that of normal mice and the oral administration of R&B Balance<sup>®</sup> could change the structure of gut microflora. These findings also suggest that R&B Balance<sup>®</sup> may improve colitis by inhibition of the production of harmful enzymes or toxic metabolites in intestine and influence the intestinal microbial diversity in DSS-induced colitic mice.