http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Wei Xue,Hang Wang,Zuodi Pei,Wei Zhao,Yong-Sheng Tian,Ri-He Peng,Quan-Hong Yao 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.3
Pichia pastoris is a suitable host for selecting mutant enzymes, since it can secret many gene products to the medium. However, poor transformation efficiency is often encountered. This makes it difficult to obtain a large number of transformants necessary to thoroughly cover a large library. We report here that pre-coating DNA with polyethyleneimine and dextran sulfate increased gene integration significantly in Pichia electroporation. This improvement on integrative efficiency has been proved in different voltage, resistance, cell phase, and DNA concentrations. The condensed DNA nanoparticles make Pichia available as a host for screening large libraries of random mutants.
Biology-Signal Analysis Software Implemented by Java
Xue-Wei Tian,Joon S. Lim(임준식) 한국지능시스템학회 2011 한국지능시스템학회 학술발표 논문집 Vol.21 No.1
The method for analysing the heart rate variability (HRV) has been adopted for assessment of health. In this paper, a summary of computer program for advanced HRV analysis which has calculated all the commonly used time and frequency domain measures of HRV as well as the nonlinear Poincare plot has been presented. The program was originally developed by using eclipse 3.4.2 based on JDK 1.6 and the generated results can be saved as a format of Microsoft Excel, which could be more convenient for further heart disease diagnostic analysis.
Comparison of HRV Time and Frequency Domain Features for Myocardial Ischemia Detection
전설위(Xue-Wei Tian),장진흥(Zhen-Xing Zhang),이상홍(Sang-Hong Lee),임준식(Joon S. Lim) 한국콘텐츠학회 2011 한국콘텐츠학회논문지 Vol.11 No.3
심박 변이도 (HRV) 분석은 심근허혈 (MI)를 평가하기 위한 편리한 도구이다. HRV에 대한 분석법은 시간 영역과 주파수 영역 분석으로 나눠질 수 있다. 본 논문은 단기간의 HRV 분석에 있어서 웨이블릿 변환을 주파수 영역 분석과 시간 영역 분석 비교하기 위하여 사용하였다. ST-T와 정상 에피소드는 각각 European ST-T 데이터베이스와 MIT-BIH Normal Sinus Rhythm 데이터베이스에서 각각 수집되었다. 한 에피소드는 32개 연속하는 RR 간격으로 나눠질 수 있다. 18개 HRV 특징은 시간과 주파수 영역 분석을 통하여 추출된다. 가종 퍼지소속함수 신경망 (NEWFM)은 추출된 18개의 특징을 이용하여 심근허혈을 진단하였다. 결과는 보여주는 평균 정확도로부터 시간영역과 주파수영역의 특징은 각각 75.29%와 80.93%이다. Heart Rate Variability (HRV) analysis is a convenient tool to assess Myocardial Ischemia (MI). The analysis methods of HRV can be divided into time domain and frequency domain analysis. This paper uses wavelet transform as frequency domain analysis in contrast to time domain analysis in short term HRV analysis. ST-T and normal episodes are collected from the European ST-T database and the MIT-BIH Normal Sinus Rhythm database, respectively. An episode can be divided into several segments, each of which is formed by 32 successive RR intervals. Eighteen HRV features are extracted from each segment by the time and frequency domain analysis. To diagnose MI, the Neural Network with Weighted Fuzzy Membership functions (NEWFM) is used with the extracted 18 features. The results show that the average accuracy from time and frequency domain features is 75.29% and 80.93%, respectively.
Analysis the role of arabidopsis CKRC6/ASA1 in auxin and cytokinin biosynthesis
Dong-Wei Di,Lei Wu,Pan Luo,Li Zhang,Tian-Zi Zhang,Xue Sun,Shao-Dong Wei,Chen-Wei An,Guang-Qin Guo 한국식물학회 2016 Journal of Plant Biology Vol.59 No.2
The crosstalk between auxin and cytokinin (CK) is important for plant growth and development, although the underlying molecular mechanisms remain unclear. Here, we describe the isolation and characterization of a mutant of Arabidopsis Cytokinin-induced Root Curling 6 (CKRC6), an allele of ANTHRANILATE SYNTHASE ALPHA SUBUNIT 1 (ASA1) that encodes the á-subunit of AS in tryptophan (Trp) biosynthesis. The ckrc6 mutant exhibits root gravitropic defects and insensitivity to both CK and the ethylene precursor 1-aminocyclopropane-1-carboxylicacid (ACC) in primary root growth. These defects can be rescued by exogenous indole-3-acetic acid (IAA) or tryptophan (Trp) supplementation. Furthermore, our results suggest that the ckrc6 mutant has decreased IAA content, differential expression patterns of auxin biosynthesis genes and CK biosynthesis isopentenyl transferase (IPT) genes in comparison to wild type. Collectively, our study shows that auxin controls CK biosynthesis based on that CK sensitivity is altered in most auxin-resistant mutants and that CKs promote auxin biosynthesis but inhibit auxin transport and response. Our results also suggest that CKRC6/ASA1 may be located at an intersection of auxin, CK and ethylene metabolism and/or signaling.
Temperature Response to Tensile Characteristics of the Hot Asphalt Mixtures
Wei Si,Ning Li,Biao Ma,Yu-xiang Tian,Xue-yan Zhou 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.4
Asphalt concrete is one type of the temperature sensitive mixtures, which is the most and broadest used pavement material in the world. Asphalt pavement suffers from the impact of temperature due to the temperature variation in different districts and environments. Facing temperature sensitivity, this paper uses Indirect Tensile Test (IDT) to analyze the tensile characters of asphalt mixture under different temperatures (-20°C, -10°C, 0°C, 15°C, 20°C, 45°C, and 60°C). The influence factors of temperature, loading rate, asphalt content, asphalt types, and mixtures gradation are evaluated. Piecewise linear model, logarithm linear model and S-logistic nonlinear model are applied to simulate the variation of flexural tensile characters with temperature change. Results show that temperature has significant influence on flexural tensile properties. Loading rates also has obvious influence on flexural tensile characteristics. With temperature changes, asphalt types have evident influence on mixture’s tensile characteristics, SBR modified asphalt has better performance at the lower temperature environment. Mixtures’ gradation has important effect on tensile strength and tensile strain as well, while due the similarity between two gradations, it is hard to precisely recommend which one is better. Asphalt content has evident influence on mixtures properties at the same temperature condition. Research results provide suggestions and applications for construction and performance of asphalt pavement. The results also indicate that IDT would be a practical option to evaluate the properties of asphalt mixture in all temperature ranges.