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Lipase-catalyzed Production of Solid Fat Containing Conjugated Linoleic Acid in Binary Models
Xue-Mei Zhu,Md. Abdul Alim,Jiang-Ning Hu,Prakash Adhikari,Jeung-Hee Lee,Ki-Teak Lee 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3
Solid fats were esterified with solid phase of rice bran oil (S-RBO), palm stearin (PS), and conjugated linoleic acid (CLA) at 2 substrate mole ratios (S-RBO:PS:CLA of 1:1:2 and 1:3:4). The major fatty acids were palmitic, oleic, and CLA in 36 hr products. The solid fat content (SFC) of the 1:1:2 product was 12.8% while the SFC of 1:3:4 product was 45.1% at 20℃. The SFCs after 20℃ reduced when the reaction time increased from 1 to 36 hr, suggesting that the change of triacylglycerol species was augmented by extending reaction time.
Xue-Mei Zhu,Jiang-Ning Hu,이정희,Yang Dan,이기택 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5
The oxidative stabilities of enzymatic modifiedlipids (EMLs) were studied using pine nut oil (PN) andpalm stearin (PS) as substrates (w/w, 40:60; 30:70) vs. aphysical blend (PB). The amount of pinolenic acid (PLA)at the sn-2 position in EML was increased to 5.5%compared to 0.8% in PB. The acid, peroxide, p-anisidine,and TBARS values were used to measure the oxidativestability of EML and PB. After 30 days of oxidation, only1.5 and 0.7% of total PLA at sn-2 position PLA weredetected in EML of PN:PS 40:60 and PN:PS 40:60,respectively. EMLs were more vulnerable to oxidizationthan PB. Different concentrations of rosemary (1,000 and2,000 mg/kg) and catetchin (400 and 800 mg/kg) wereadded to EMLs to reduce the oxidation level. Resultsindicated that these two antioxidants deterred the extent ofoxidation in EMLs.
Determination of Tocopherol Contents in Refined Edible Oils Using an HPLC Method
Hu, Jiang-Ning,Zhu, Xue-Mei,Adhikari, Prakash,Li, Dan,Kim, In-Hwan,Lee, Ki-Teak The Korean Society of Food Science and Nutrition 2009 Preventive Nutrition and Food Science Vol.14 No.3
A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression ($r^2$>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94$\sim$4.27 and 1.77$\sim$ 4.88%, respectively. The tocopherol recoveries ranged from 91.44$\sim$108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of $\alpha$, $\gamma$, and $\delta$-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.
Compaction techniques and construction parameters of loess as filling material
Hu, Chang-Ming,Wang, Xue-Yan,Mei, Yuan,Yuan, Yi-Li,Zhang, Shan-Shan Techno-Press 2018 Geomechanics & engineering Vol.15 No.6
Loess often causes problems when used as a filling material in the construction of foundations. Therefore, the compaction technique, shear behavior, and bearing capacity of a filled foundation should be carefully considered. A series of tests was performed in this study to obtain effective compaction techniques and construction parameters. The results indicated that loess is strongly sensitive to water. Thus, the soil moisture content should be kept within 12%-14% when it is used as a filling material. The vibrating-dynamic combination compaction technique is effective and has fewer limitations than other methods. In addition, the shear strength of the compacted loess was found to increase linearly with the degree of compaction, and the soil's compressibility decreased rapidly with an increase in the degree of compaction when the degree of compaction was less than 95%. Finally, the characteristic value of the bearing capacity increased with an increase in the degree of compaction in a ladder-type way when the degree of compaction was within 92%-95%. Based on the test data, this paper could be used as a reference in the selection of construction designs in similar engineering projects.
Xue-Mei Zhu,Jiang-Ning Hu,Jung-Ah Shin,Jeung-Hee Lee,Soon-Teak Hong,Ki-Teak Lee 한국식품영양과학회 2010 Preventive Nutrition and Food Science Vol.15 No.4
The chemical characteristics of seed oils of Asian ginseng (Panax ginseng C.A. Meyer) at different ages grown in Korea (3, 4 and 5-year old) and China (5-year old), and American ginseng (Panax quinquefoliu L., 5-year old) grown in China were compared. Total fatty acid composition showed a significantly higher oleic acid content in American (87.50%) than in Korean (68.02~69.14%) and Chinese ginseng seed oils (61.19%). At the sn-2 position, the highest oleic acid (81.09%) and lowest linoleic acid (15.77%) were found in American ginseng seed oil. The main triacylglycerol species in ginseng seed oils were triolein (OOO) and 1,2-dioleoyl-3-linoleoyl-glycerol (LOO)/1,3-dioleoyl-2-linoleoyl-glycerol (OLO). In addition, the seed oils possessed an ideal oxidative stability showing 16.55~23.12 hr of induction time by Rancimat test. The results revealed that ginseng seed oil could be developed as a new healthy edible oil, and that the oil’s chemical characteristics were strongly associated with the ginseng species and habitats.
Xue-Lian Zhao,Shang-Ying Hu,Qian Zhang,Li Dong,Rui-Mei Feng,Ross Han,Fang-Hui Zhao 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.4
Objective: To explore the genotype distribution of high-risk human papillomavirus (HR-HPV) and its attribution to different grades of cervical lesions in rural China, which will contribute to type-specific HPV screening tests and the development of new polyvalent HPV vaccines among the Chinese population. Methods: One thousand two hundred ninety-two subjects were followed based on the Shanxi Province Cervical Cancer Screening Study I (SPOCCS-I), and screened by HPV DNA testing (hybrid capture® 2 [HC2]), liquid-based cytology (LBC), and if necessary, directed or random colposcopy-guided quadrant biopsies. HPV genotyping with linear inverse probe hybridization (SPF10-PCR-LiPA) was performed in HC2 positive specimens. Attribution of specific HR-HPV type to different grades of cervical lesions was estimated using a fractional contribution approach. Results: After excluding incomplete data, 1,274 women were included in the final statistical analysis. Fifteen point two percent (194/1,274) of women were HR-HPV positive for any of 13 HR-HPV types (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68) and the most common HR-HPV types were HPV16 (19.1%) and HPV52 (16.5%). The genotypes most frequently detected in HR-HPV-positive cervical intraepithelial neoplasia grade 1 (CIN1) were HPV52 (24.1%), HPV31 (20.7%), HPV16 (13.8%), HPV33 (13.8%), HPV39 (10.3%), and HPV56 (10.3%); in HR-HPV-positive cervical intraepithelial neoplasia grade 2 or worse (CIN2+): HPV16 (53.1%), HPV58 (15.6%), HPV33 (12.5%), HPV51 (9.4%), and HPV52 (6.3%). HPV52, 31, 16, 33, 39, and 56 together contributed to 89.7% of HR-HPV-positive CIN1, and HPV16, 33, 58, 51, and 52 together contributed to 87.5% of CIN2+. Conclusion: In summary, we found substantial differences in prevalence and attribution of CINs between different oncogenic HPV types in a rural Chinese population, especially for HPV16, 31, 33, 52, and 58. These differences may be relevant for both clinical management and the design of preventive strategies.
Isoflavanones from the Stem of Cassia siamea and Their Anti-tobacco Mosaic Virus Activities
Hu, Qiu-Fen,Niu, De-Yun,Zhou, Bin,Ye, Yan-Qing,Du, Gang,Meng, Chun-Yang,Gao, Xue-Mei Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.10
Two new isoflavanones, (3R) 7,2',4'-trihydroxy-3'-methoxy-5-methoxycarbonyl-isoflavanone (1) and (3R) 7,2'-dihydroxy-3',4'-dimethoxy-5-methoxycarbonyl-isoflavanone (2), together with six known isoflavanones (3-8), were isolated from the stems of Cassia siamea. The structure of 1-8 was elucidated by spectroscopic methods including extensive 1D- and 2D-NMR techniques. Compounds 1, 2, 5-8 were evaluated for their anti-tobacco mosaic virus (Anti-TMV) activity. The results showed that compounds 1 and 6 showed potential anti-TMV activity with inhibition rates of 24.6% and 26.9%, respectively. Compounds 2, 5, 7, 8 also showed anti-TMV activity with inhibition rates in the range of 11.8-18.6%.