http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
DnaJ of Streptococcus suis Type 2 Contributes to Cell Adhesion and Thermotolerance
Zhang, Xiaoyan,Jiang, Xiaowu,Yang, Ling,Fang, Lihua,Shen, Hongxia,Lu, Xingmeng,Fang, Weihuan The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6
To examine if the molecular chaperone DnaK operon proteins of Streptococcus suis type 2 (SS2) are involved in adhesion to host cells, the abundance values of these proteins from the surface of two SS2 strains of different adhesion capability were compared. Their roles in growth and adhesion to human laryngeal epithelial cell line HEp-2 cells were investigated on SS2 strain HA9801 and its mutants with DnaK operon genes partially knocked-out (PKO mutant) under heat stress. The major difference was that DnaJ was more abundant in strain HA9801 than in strain JX0811. Pretreatment of the bacteria with hyperimmune sera to DnaJ, but not with those to other proteins, could significantly reduce SS2 adhesion to HEp-2 cells. PKO of dnaJ g ene resulted in decreased SS2 growth at 37℃ and 42℃, and reduced its adhesion to HEp-2 cells. The wild-type strain stressed at 42℃ had increased expression of DnaJ on its surface and elevated adhesion to HEp-2 cells, which was also inhibitable by DnaJ specific antiserum. These results indicate that the DnaJ of S. suis type 2 is important not only for thermotolerance but also for adhesion to host cells. Because DnaJ expression is increased upon temperature upshift with increased exposure on the bacterial surface, the febrile conditions of the cases with systemic infections might help facilitate bacterial adhesion to host cells. DnaJ could be one of the potential candidates as a subunit vaccine because of its good immunogenicity.
Yingzhong Shen,Xiaoyan Wang,Sen Xu,Hulin Li 한국물리학회 2007 Current Applied Physics Vol.7 No.1
2InL [L= N-(4-methoxy)benzylidenethiobenzahydrazonato (1),N-(3,4-dimethoxy)benzyl-idenethiobenzahydrazonato (2),N-(4-N,N-dimethylamino)benzylidenethiobenzahydrazonato (3),N-(2-naphthyl)methylene thiobenza-hydrazonato (4) and N-(9-anthryl)methylenethiobenzahydrazonato (5)] have been synthesized by reaction of trimethylindium withappropriateN-arylmethylenethiobenzahydrozones. The complexes obtained have been characterized by elemental analysis,1H NMR,IR and mass spectroscopy. Compounds15 emit blue colors atkmax = 432479 nm when irradiated by UV light. The electroluminescent(EL) properties of15 were examined by fabricating EL devices using15 region (513578 nm).
Huang Xi,Shen Yinzhong,Li Qingshan,Li Xiaoyan,Zhan Zixiong,Li Guang,Li Zhenhe 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.7
The nanoindentation behavior of P92 steel with thermomechanical treatment under 3.5 MeV Fe13þ ion irradiation at room temperature, 400 and 700 C was investigated. Pop-in behavior is observed for all the samples with and without irradiation at room temperature, while the temperature dependence of pop-in behavior is only observed in irradiated samples. The average load and penetration depth at the onset of pop-in increase as the irradiation temperature increases, in line with the results of the maximum shear stress. Irradiation induced hardening is exhibited for all irradiated samples, but there is a significant reduction in the hardness of sample irradiated at 700 C in comparison to the samples irradiated at room temperature and 400 C. The ratio of hardness to elastic modulus for all samples decreases with increasing penetration depth except for samples at 700 C. With the increasing of irradiation temperature, the ratio of the irreversible work to the total work gradually decreases. In contrast, it increases for samples without irradiation
Meng-Wen Wu,Xiaoyan Wang,Sijia Qiao,Chung-Hua Shen,Juan Wang 한국증권학회 2022 Asia-Pacific Journal of Financial Studies Vol.51 No.2
This study investigates the effect of non-financial firms’ activities in shadow banking on firm risk and performance. Using manually collected data of entrusted loans from Chinese listed firms, we find that lending firms’ bankruptcy risk and performance increases from their engagement of entrusted loan businesses in the year the loans are issued and in the following year. Further, firms’ risk increases and performance improves significantly when firms are financially healthy, financially constrained, and non-state-owned. Overall, our findings provide policy implications that the risk of shadow banking activities must be cautious.
Huimin Yang,Guoli Liu,Shubin He,Yuying Shen,Xiaoyan Zhang 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
Forage production and water use efficiency (WUE) of Lucerne were investigated in three varieties at different water availabilities. Forage production decreased with the severity of soil water availability. At 50% field water capacity (FWC), forage production dropped but in two varieties, Algonquin and Longdong, was still high and from 75%FWC to 50%FWC, forage production in Longdong decreased at the least rate. The greatest leaf WUE was observed in Longdong at all soil water availabilities. From 75%FWC to 50%FWC, it increased in Longdong and Xinjiangdaye, but decreased in Algonquin. With the severity of water deficit, δ¹³C value increased in all three varieties. At the same water availability, the greatest value was observed in Longdong. It suggested that moderate water stress can improve WUE in Lucerne. Longdong is to some extent more efficient in water use and may be more drought-tolerant with more steady production at moderate water deficit.
Yuqian Hu,Linlin Zheng,Jinhui Zhang,Lijuan Lin,Yue Shen,Xiaoyan Zhang,Buling Wu 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.5
Background: In bone tissue engineering, the fabrication and biocompatibility of scaffold are crucial. Among many scaffold materials, nanohydroxyapatite (nHAP) and collagen (COL) are chosen as building materials of scaffold. At the same time, growth factors were also used to modify the scaffolds. Methods: In this study, blending and freeze drying methods were adopted together in order to build basic fibroblast growth factor (bFGF)-bone morphogenetic protein-2 (BMP-2)-nHAP/COL scaffolds. ELISA was applied to test the release of bFGF and BMP-2 on the scaffold. The flow cytometry was used to identify bone marrow mesenchymal stem cells (BMSCs). Scanning electron microscope was adopted to observe scaffolds and cells morphology. BMSCs were seeded on the scaffolds to test the biological compatibility in vitro. Cells were counted to detect early cell adhesion. Cell counting kit-8 assay was adopted to detect cell proliferation and alkalinephosphatase assay was applied to detect cell activity. Results: The characterization of bFGF-BMP-2-nHAP/COL scaffolds meets the requirements of ideal bone tissue engineering scaffolds. BMSCs that were isolated, purified and passaged satisfied the needs of further experiments. The growth status of cells on bFGF-BMP-2-nHAP/COL scaffolds was satisfactory. Cell adhesion was the highest in the bFGF-BMP-2-nHAP/COL scaffolds group. The cell viability and ALP activity of bFGF-BMP-2-nHAP/COL scaffolds group were the highest. Conclusion: Taken together, bFGF-BMP-2-nHAP/COL scaffolds have good biocompatibility in vitro and promote adhesion, proliferation, differentiation of BMSCs.
Jianlong Yin,Jing Yu,Cheng Lou,Dongsheng Li,Xiaoyan Shen,Ming Li 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.2
In an aerostatic restrictor, the presence of friction between the high-pressure air and the aerostatic restrictor wall can lead to non-uniform pressure and temperature distributions of the lubricating air film, which will affect the aerostatic restrictor performance. Therefore, explicating the pressure and temperature distributions of the lubricating air film could help to improve the characteristics and performance of the aerostatic restrictor. In this study, a theoretical model is established for the pressure and temperature distributions in the aerostatic restrictor with double U-shaped pressure-equalizing grooves, which is then compared with simulations, and finally verified by experiments. The results indicate the following. (1) The parameter distributions of the aerostatic restrictor’s air film are affected by the pressure-equalizing grooves and the orifice. (2) The air film parameter distributions are affected by the air source pressure and air film thickness. (3) The air film parameter distribution characteristics obtained from theoretical calculations, simulations, and experiments are consistent, indicating that the theoretical model is effective for studying the flow field of lubricating air film in the aerostatic restrictor. This model can also provide a theoretical basis for designing the orifice and pressure-equalizing grooves of the aerostatic restrictor, as well as selecting the air source pressure and air film thickness.
Liuxin Xiang,Yuxian Xia,Ying-Fan Cai,Jijun Liu,Xiaohong He,Quan Sun,Xiaoyan Wang,Yuyin Fu,Yonghong Fan,Daiwen Dong,Guanfan Zhou,Jinjuan Shen,Yihua Liu 한국식물학회 2013 Journal of Plant Biology Vol.56 No.3
The first tuber mustard calmodulin-like (CML) gene BjAAR1 (Brassica juncea var. tumida Tsen et Lee Abiotic stress and Abscisic acid (ABA) Responsive gene 1) was cloned and characterized. The protein encoded by BjAAR1 contains four predicted Ca2+ binding sites (EF-hand motif) and its recombinant protein can bind Ca2+ in vitro. qRT-PCR showed that the expression level of BjAAR1 was rather high in non-swollen stem of tuber mustard and largely reduced in swollen stem. Expression of BjAAR1 enhanced ABA- and stress-induced gene expression in Arabidopsis (Arabidopsis thaliana). Transgenic plants also exhibited hypersensitivity to NaCl, mannitol, and ABA during the seed germination and post-germination stages. ABA biosynthesis inhibitor, norflurazon (NF), rescued hypersensitivity phenotype of transgenic plants to NaCl and mannitol, indicating that BjAAR1 functions in multiple abiotic stresses response through ABA-dependent process.