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        Interaction of Pseudostellaria heterophylla with Quorum Sensing and Quorum Quenching Bacteria Mediated by Root Exudates in a Consecutive Monoculture System<sup>s</sup>

        ( Liaoyuan Zhang ),( Zewang Guo ),( Huifang Gao ),( Xiaoqian Peng ),( Yongyu Li ),( Shujing Sun ),( Jung-kul Lee ),( Wenxiong Lin ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

        Many plant-pathogenic bacteria are dependent on quorum sensing (QS) to evoke disease. In this study, the population of QS and quorum quenching (QQ) bacteria was analyzed in a consecutive monoculture system of Pseudostellaria heterophylla. The isolated QS strains were identified as Serratia marcescens with SwrIR-type QS system and exhibited a significant increase over the years of monoculture. Only one QQ strain was isolated from newly planted soil sample and was identified as Bacillus thuringiensis, which secreted lactonase to degrade QS signal molecules. Inoculation of S. marcescens to P. heterophylla root could rapidly cause wilt disease, which was alleviated by B. thuringiensis. Furthermore, the expression of lactonase encoded by the aiiA gene in S. marcescens resulted in reduction of its pathogenicity, implying that the toxic effect of S. marcescens on the seedlings was QS-regulated. Meanwhile, excess lactonase in S. marcescens led to reduction in antibacterial substances, exoenzymes, and swarming motility, which might contribute to pathogensis on the seedlings. Root exudates and root tuber extracts of P. heterophylla significantly promoted the growth of S. marcescens, whereas a slight increase of B. thuringiensis was observed in both samples. These results demonstrated that QS-regulated behaviors in S. marcescens mediated by root exudates played an important role in replanting diseases of P. heterophylla.

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        Study of Deformation Mechanisms of a High-Purity α-Titanium Plate Under Monotonic Loading with the EVPSC-TDT Model

        Chao Ma,Xing Duan,Xiaoqian Guo,Hua Qiao,Lianying Zhang,Xianbiao Mao,Peidong Wu 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.2

        The deformation mechanisms of a rolled high-purity α-titanium plate under monotonic loading along the rolling direction(RD), transverse direction (TD), and normal direction (ND) are investigated by the Elastic Visco-Plastic Self-Consistent(EVPSC) model, which incorporates a Twinning and De-Twinning (TDT) scheme to describe twinning behavior duringstraining. In the EVPSC-TDT model, plastic deformation is assumed to be accommodated by prismatic, basal and pyramidal⟨c + a⟩ slip modes as well as the 10̄12 extension and 11̄22 contraction twin modes. Numerical results based on theEVPSC-TDT model are in good agreement with the corresponding experimental data. The tension–compression asymmetry,anisotropic initial yielding and strain hardening behavior are interpreted in terms of the predicted relative activities of variousdeformation modes, twin volume fractions and texture evolutions. It is demonstrated that twinning plays an important rolein tension–compression asymmetry and plastic anisotropy, which is closely related to the loading direction with respect tocrystal orientations in the initial texture. In addition, it can be concluded that the TDT scheme permits better performancein describing twinning-associated deformation behavior for the rolled high-purity α-titanium plate than the PredominantTwin Reorientation (PTR) model even when detwinning is not involved.

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        White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment

        Zhu Qinchao,Zhou Xuhao,Zhang Yanan,Ye Di,Yu Kang,Cao Wangbei,Zhang Liwen,Zheng Houwei,Sun Ziyang,Guo Chengchen,Hong Xiaoqian,Zhu Yang,Zhang Yajun,Xiao Ying,Valencak Teresa G.,Ren Tanchen,Ren Daxi 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

        Background : Post-traumatic massive hemorrhage demands immediately available first-aid supplies with reduced operation time and good surgical compliance. In-situ crosslinking gels that are flexibly adapting to the wound shape have a promising potential, but it is still hard to achieve fast gelation, on-demand adhesion, and wide feasibility at the same time. Methods : A white-light crosslinkable natural milk-derived casein hydrogel bioadhesive is presented for the first time. Benefiting from abundant tyrosine residues, casein hydrogel bioadhesive was synthesized by forming di-tyrosine bonds under white light with a ruthenium-based catalyst. We firstly optimized the concentration of proteins and initiators to achieve faster gelation and higher mechanical strength. Then, we examined the degradation, cytotoxicity, tissue adhesion, hemostasis, and wound healing ability of the casein hydrogels to study their potential to be used as bioadhesives. Result : Rapid gelation of casein hydrogel is initiated with an outdoor flashlight, a cellphone flashlight, or an endoscopy lamp, which facilitates its usage during first-aid and minimally invasive operations. The rapid gelation enables 3D printing of the casein hydrogel and excellent hemostasis even during liver hemorrhage due to section injury. The covalent binding between casein and tissue enables robust adhesion which can withstand more than 180 mmHg blood pressure. Moreover, the casein-based hydrogel can facilitate post-traumatic wound healing caused by trauma due to its biocompatibility. Conclusion : Casein-based bioadhesives developed in this study pave a way for broad and practical application in emergency wound management.

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