Homogeneous preparation of polyamidoamine grafted silica gels and their adsorption properties as Au3+ adsorbents

Rongjun Qu,Xilu Ma,Minghua Wang,Changmei Sun,Xiaomei Sun,Shaoying Sun,Ying Zhang,Ping Yin 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6

A series of low generation dendrimer polyamidoamine grafted silica gels (named SiO2-G1.0, SiO2-G2.0,SiO2-G3.0) were synthesized via the homogeneous route. The new materials were characterized byscanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermal analysis,pore and surface area analysis, X-ray diffraction analysis (XRD) and elemental analysis. The adsorptionproperties of the three products for Au3+ were studied by batch methods. The adsorbents prepared viathe homogeneous route showed higher adsorption capacities than those via the heterogeneous route. The maximum adsorption capacities for the three could reach up to 1.48, 2.45, and 2.21 mmol g-1,respectively.

A Novel Perceptual Hashing for Color Images Using a Full Quaternion Representation

( Xiaomei Xing ),( Yuesheng Zhu ),( Zhiwei Mo ),( Ziqiang Sun ),( Zhen Liu ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.12

Quaternions have been commonly employed in color image processing, but when the existing pure quaternion representation for color images is used in perceptual hashing, it would degrade the robustness performance since it is sensitive to image manipulations. To improve the robustness in color image perceptual hashing, in this paper a full quaternion representation for color images is proposed by introducing the local image luminance variances. Based on this new representation, a novel Full Quaternion Discrete Cosine Transform (FQDCT)-based hashing is proposed, in which the Quaternion Discrete Cosine Transform (QDCT) is applied to the pseudo-randomly selected regions of the novel full quaternion image to construct two feature matrices. A new hash value in binary is generated from these two matrices. Our experimental results have validated the robustness improvement brought by the proposed full quaternion representation and demonstrated that better performance can be achieved in the proposed FQDCT-based hashing than that in other notable quaternion-based hashing schemes in terms of robustness and discriminability.

중국의 여성학

��, 샤오메이,이인허 이화여자대학교 한국여성연구원 1998 여성학논집 Vol.14-15 No.-

MicroRNA-362 Inhibits Cell Proliferation and Invasion by Directly Targeting SIX1 in Colorectal Cancer

Jin’e Wan,Jian Yang,Cuixia Qiao,Xiaomei Sun,Aiting Di,Lize Zhang,Dandan Wang,Gang Zhao 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.5

Purpose: Colorectal cancer (CRC) is the third most common cancer in China and poses high morbidity and mortality. In recentyears, increasing evidence has indicated that microRNAs played important functions in the occurrence and development of tumors. The purpose of this study was to identify the biological mechanisms of miR-362 in CRC. Materials and Methods: Quantitative real-time PCR was carried out to assess the expression of miR-362 and SIX1. The Kaplan-Meier method was employed to evaluate the 5-year overall survival of CRC patients. The proliferative and invasive abilities of CRCcells were assessed by MTT and transwell assays. Results: miR-362 was significantly decreased in CRC tissues and cell lines, compared to the normal tissues and normal cells. Asignificant connection was confirmed between the overall survival of 53 CRC patients and low expression of miR-362. Downregulationof miR-362 inhibited the proliferation and invasion through binding to the 3'-UTR of SIX1 mRNA in CRC. Additionally, wediscovered that SIX1 was a direct target gene of miR-362 and that the expression of miR-362 had a negative connection with SIX1expression in CRC. SIX1 could reverse partial functions in the proliferation and invasion in CRC cells. Conclusion: miR-362 may be a prognostic marker in CRC and suppress CRC cell proliferation and invasion in part through targetingthe 3'-UTR of SIX1 mRNA. The newly identified miR-362/SIX1 axis provides insight into the progression of CRC.

Crack Detection Based on Generative Adversarial Networks and Deep Learning

Gongfa Chen,Shuai Teng,Mansheng Lin,Xiaomei Yang,Xiaoli Sun 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4

This paper proposes a novel crack detection method using the three-stages detection model. Deep learning technology has been a focus of attention in the field of crack detection; however, it needs big data to train the corresponding network model. More training samples and the combination of multiple deep learning algorithms help to improve the detection performance. Therefore, this paper employed a generative adversarial network (GAN) model to generate abundant virtual crack images with similar features to real images, these virtual images are used to train the CNN classifier and DeepLab_v3+ respectively, and then the real images are used to evaluate the performance of the three-stages detection method. The results show that the proposed three-stages detection method has excellent detection effect on the crack detection is better than that of the control experiment (the NI_MIoU, NI_Accuracy, NI_F-score and NI_MCC are increased by 22.1% − 55.6%, 5.2% − 9.8%, 37.4% − 40.0% and 6.2% − 11.1%, respectively)). These results demonstrate that the three-stages detection model has made a beneficial contribution to the crack detection.

Direct Electrochemistry and Electrocatalysis of Myoglobin with CoMoO4 Nanorods Modified Carbon Ionic Liquid Electrode

Zengying Zhao,Lili Cao,Anhui Hu,Weili Zhang,Xiaomei Ju,Yuanyuan Zhang,Wei Sun 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

By using ionic liquid 1-hexylpyridinium hexafluorophosphate (HPPF6) based carbon ionic liquid electrode (CILE) as the substrate electrode, a CoMoO4 nanorods and myoglobin (Mb) composite was casted on the surface of CILE with chitosan (CTS) as the film forming material to obtain the modified electrode (CTS/ CoMoO4-Mb/CILE). Spectroscopic results indicated that Mb retained its native structures without any conformational changes after mixed with CoMoO4 nanorods and CTS. Electrochemical behaviors of Mb on the electrode were carefully investigated by cyclic voltammetry with a pair of well-defined redox peaks from the heme Fe(III)/Fe(II) redox center of Mb appeared, which indicated that direct electron transfer between Mb and CILE was realized. Electrochemical parameters such as the electron transfer number (n), charge transfer coefficient (α) and electron transfer rate constant (ks) were estimated by cyclic voltammetry with the results as 1.09, 0.53 and 1.16 s−1, respectively. The Mb modified electrode showed good electrocatalytic ability toward the reduction of trichloroacetic acid in the concentration range from 0.1 to 32.0 mmol L−1 with the detection limit as 0.036 mmol L−1 (3σ), and the reduction of H2O2 in the concentration range from 0.12 to 397.0 μmol L− 1 with the detection limit as 0.0426 μmol L−1 (3σ).

Direct Electrochemistry and Electrocatalysis of Myoglobin with CoMoO₄ Nanorods Modified Carbon Ionic Liquid Electrode

Zhao, Zengying,Cao, Lili,Hu, Anhui,Zhang, Weili,Ju, Xiaomei,Zhang, Yuanyuan,Sun, Wei Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

By using ionic liquid 1-hexylpyridinium hexafluorophosphate ($HPPF_6$) based carbon ionic liquid electrode (CILE) as the substrate electrode, a $CoMoO_4$ nanorods and myoglobin (Mb) composite was casted on the surface of CILE with chitosan (CTS) as the film forming material to obtain the modified electrode (CTS/$CoMoO_4$-Mb/CILE). Spectroscopic results indicated that Mb retained its native structures without any conformational changes after mixed with $CoMoO_4$ nanorods and CTS. Electrochemical behaviors of Mb on the electrode were carefully investigated by cyclic voltammetry with a pair of well-defined redox peaks from the heme Fe(III)/Fe(II) redox center of Mb appeared, which indicated that direct electron transfer between Mb and CILE was realized. Electrochemical parameters such as the electron transfer number (n), charge transfer coefficient (${\alpha}$) and electron transfer rate constant ($k_s$) were estimated by cyclic voltammetry with the results as 1.09, 0.53 and 1.16 $s^{-1}$, respectively. The Mb modified electrode showed good electrocatalytic ability toward the reduction of trichloroacetic acid in the concentration range from 0.1 to 32.0 mmol $L^{-1}$ with the detection limit as 0.036 mmol $L^{-1}$ ($3{\sigma}$), and the reduction of $H_2O_2$ in the concentration range from 0.12 to 397.0 ${\mu}mol\;L^{-1}$ with the detection limit as 0.0426 ${\mu}mol\;L^{-1}$ ($3{\sigma}$).

Serum exosomal miR-192 serves as a potential detective biomarker for early pregnancy screening in sows

Gao Ruonan,Li Qingchun,Qiu Meiyu,Xie Su,Sun Xiaomei,Huang Tao 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.9

Objective: The study was conducted to screen differentially expressed miRNAs in sows at early pregnancy by high-throughput sequencing and explore its mechanism of action on embryo implantation. Methods: The blood serum of pregnant and non-pregnant Landrace×Yorkshire sows were collected 14 days after artificial insemination, and exosomal miRNAs were purified for high throughput miRNA sequencing. The expression patterns of 10 differentially expressed (DE) miRNAs were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR quantified the abundance of serum exosomal miR-192 in pregnant and control sows, and the diagnostic power was assessed by receiver operating characteristic (ROC) analysis. The target genes of DE miRNAs were predicted with bioinformatics software, and the functional and pathway enrichment analysis was performed on gene ontology and the Kyoto encyclopedia of genes and genomes terms. Furthermore, a luciferase reporter system was used to identify the target relation between miR-192 and integrin alpha 4 (ITGA4), a gene influencing embryo implantation in pigs. Finally, the expression levels of miRNAs and the target gene ITGA4 were analyzed by qRT-PCR, and western blot, with the proliferation of BeWo cells detected by cell counting kit-8 (CCK-8). Results: A total of 221 known miRNAs were detected in the libraries of the pregnant and non-pregnant sows, of which 55 were up-regulated and 67 were down-regulated in the pregnant individuals compared with the non-pregnant controls. From these, the expression patterns of 10 DE miRNAs were validated. The qRT-PCR analysis further confirmed a significantly higher expression of miR-192 in the serum exosomes extracted from pregnant sows, when compared to controls. The ROC analysis revealed that miR-192 provided excellent diagnostic accuracy for pregnancy (area under the ROC curve [AUC] = 0.843; p>0.001). The dual-luciferase reporter assay indicated that miR-192 directly targeted ITGA4. The protein expression of ITGA4 was reduced in cells that overexpressed miR-192. Overexpression of miR-192 resulted in the decreased proliferation of BeWo cells and regulated the expression of cell cycle-related genes. Conclusion: Serum exosomal miR-192 could serve as a potential biomarker for early pregnancy in pigs. miR-192 targeted ITGA4 gene directly, and miR-192 can regulate cellular proliferation.

miR-133a-3p and miR-145-5p co-promote goat hair follicle stem cell differentiation by regulating NANOG and SOX9 expression

Wang Jian,Wu Xi,Zhang Liuming,Wang Qiang,Sun Xiaomei,Ji Dejun,Li Yongjun 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.4

Objective: Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited. Methods: To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs. Results: miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145- 5p or both inhibiting can inhibit goat HFSCs differentiation. Conclusion: These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development.