MiR-126-3p inhibits apoptosis and promotes proliferation by targeting phosphatidylinositol 3-kinase regulatory subunit 2 in porcine ovarian granulosa cells

Zhou, Xiaofeng,He, Yingting,Jiang, Yao,He, Bo,Deng, Xi,Zhang, Zhe,Yuan, Xiaolong,Li, Jiaqi Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: Numerous studies have indicated that the apoptosis and proliferation of granulosa cells (GCs) are closely related to the normal growth and development of follicles and ovaries. Previous evidence has suggested that miR-126-3p might get involved in the apoptosis and proliferation of GCs, and phosphatidylinositol 3-kinase regulatory subunit 2 (PIK3R2) gene has been predicted as one target of miR-126-3p. However, the molecular regulation of miR-126-3p on PIK3R2 and the effects of PIK3R2 on porcine GCs apoptosis and proliferation remain virtually unexplored. Methods: In this study, using porcine GCs as a cellular model, luciferase report assay, mutation and deletion were applied to verify the targeting relationship between miR-126-3p and PIK3R2. Annexin-V/PI staining and 5-ethynyl-2'-deoxyuridine assay were applied to explore the effect of PIK3R2 on GCs apoptosis and proliferation, respectively. Real-time quantitative polymerase chain reaction and Western Blot were applied to explore the regulation of miR-126-3p on PIK3R2 expression. Results: We found that miR-126-3p targeted at PIK3R2 and inhibited its mRNA and protein expression. Knockdown of PIK3R2 significantly inhibited the apoptosis and promoted the proliferation of porcine GCs, and significantly down-regulated the mRNA expression of several key genes of PI3K pathway such as insulin-like growth factor 1 receptor (IGF1R), insulin receptor (INSR), pyruvate dehydrogenase kinase 1 (PDK1), and serine/threonine kinase 1 (AKT1). Conclusion: MiR-126-3p might target and inhibit the mRNA and protein expressions of PIK3R2, thereby inhibiting GC apoptosis and promoting GC proliferation by down-regulating several key genes of the PI3K pathway, IGF1R, INSR, PDK1, and AKT1. These findings would provide great insight into further exploring the molecular regulation of miR-126-3p and PIK3R2 on the functions of GCs during the folliculogenesis in female mammals.

Removal of Chromium (Ⅵ) by Escherichia coli Cells Expressing Cytoplasmic or Surface-Displayed ChrB: a Comparative Study

( Xiaofeng Zhou ),( Jianghui Li ),( Weilong Wang ),( Fan Yang ),( Bingqian Fan ),( Chenlu Zhang ),( Xiaojun Ren ),( Feng Liang ),( Rong Cheng ),( Fengying Jiang ),( Huaibin Zhou ),( Juanjuan Yang ),( 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7

Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed Ochrobactrum tritici 5bvl1 ChrB in Escherichia coli BL21(DE3) cells. E. coli cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible to variation in extracellular conditions (pH and ionic strength), and more effectively removed Cr(VI) from industrial wastewater than the surface-displayed ChrB at low pH (<3). An adsorptiondesorption experiment demonstrated that compared with intracellular accumulation, cell-surface adsorption is reversible, which allows easy desorption of the adsorbed metal ions and regeneration of the bioadsorbent. In addition, an intrinsic ChrB protein fluorescence assay suggested that pH and salinity may influence the Cr(VI) adsorption capacity of ChrB-expressing E. coli cells by modulating the ChrB protein conformation. Although the characteristics of ChrB may not be universal for all metal-binding proteins, our study provides new insights into different engineering strategies for whole-cell biosorbents for removing heavy metals from industrial effluents.

Uncertainty Analysis of Dynamic Thermal Rating of Overhead Transmission Line

Zhou, Xing,Wang, Yanling,Zhou, Xiaofeng,Tao, Weihua,Niu, Zhiqiang,Qu, Ailing Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.2

Dynamic thermal rating of the overhead transmission lines is affected by many uncertain factors. The ambient temperature, wind speed and wind direction are the main sources of uncertainty. Measurement uncertainty is an important parameter to evaluate the reliability of measurement results. This paper presents the uncertainty analysis based on Monte Carlo. On the basis of establishing the mathematical model and setting the probability density function of the input parameter value, the probability density function of the output value is determined by probability distribution random sampling. Through the calculation and analysis of the transient thermal balance equation and the steady- state thermal balance equation, the steady-state current carrying capacity, the transient current carrying capacity, the standard uncertainty and the probability distribution of the minimum and maximum values of the conductor under 95% confidence interval are obtained. The simulation results indicate that Monte Carlo method can decrease the computational complexity, speed up the calculation, and increase the validity and reliability of the uncertainty evaluation.

A Novel 3D Model Retrieval Method Based On Shape Index Distribution

Jilai Zhou,Mingquan Zhou,Guohua Geng,Xiaofeng Wang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.9

Uncertainty Analysis of Dynamic Thermal Rating of Overhead Transmission Line

Xing Zhou,Yanling Wang,Xiaofeng Zhou,Weihua Tao,Zhiqiang Niu,Ailing Qu 한국정보처리학회 2019 Journal of information processing systems Vol.15 No.2

Dynamic thermal rating of the overhead transmission lines is affected by many uncertain factors. The ambienttemperature, wind speed and wind direction are the main sources of uncertainty. Measurement uncertainty isan important parameter to evaluate the reliability of measurement results. This paper presents the uncertaintyanalysis based on Monte Carlo. On the basis of establishing the mathematical model and setting the probabilitydensity function of the input parameter value, the probability density function of the output value is determinedby probability distribution random sampling. Through the calculation and analysis of the transient thermalbalance equation and the steady- state thermal balance equation, the steady-state current carrying capacity, thetransient current carrying capacity, the standard uncertainty and the probability distribution of the minimumand maximum values of the conductor under 95% confidence interval are obtained. The simulation resultsindicate that Monte Carlo method can decrease the computational complexity, speed up the calculation, andincrease the validity and reliability of the uncertainty evaluation.

Microstructural Evolution of a High W Content Ni-Based Superalloy at γ′ Sub-Solvus Temperatures

Sigeng Zhou,Naicheng Sheng,Shijie Sun,Shigang Fan,Jinjiang Yu,Guichen Hou,Jinguo Li,Yizhou Zhou,Xiaofeng Sun 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.1

The evolution of γ′ phase and γ/γ′ eutectic during short-time heat treatment of the Ni-based superalloy with high W content,K416B are investigated. In this study, high temperature Differential Scanning Calorimetry (DSC) is employed to designthe heat treatment experiments and the precipitate temperature of secondary γ′ is found in the cooling curve. The standardcoarsening model is applied to figure out the coarsening rate constant of γ′ precipitate. On this basis, a novel method isproposed to predict the globularization time of γ phase in γ/γ′ eutectic during the heat treatment process. As holding temperatureincreases, more γ lamellae convert to platelets before reaching thermodynamic equilibrium. Electron microscopyobservations exhibit the boundary splitting and necking formation on the γ plate surface. Moreover, the dissolution kineticsof γ phase is quantitatively assessed by JMAK model. The volume fraction descends rapidly in the initial stage, and thenreach to a threshold. Above proposed approaches are validated via various heat treatment experiments.

Improving Dielectric Properties in Novel P(VDF‑HFP)/V2AlC MAX/Montmorillonite Composite Films via Interfacial Electric‑Leakage Depressing Strategy

Xiaofeng Xia,Junquan Zhou,Huimin Ding,Qin Li,Maolin Bo,Qihuang Deng,Yefeng Feng 대한금속·재료학회 2021 ELECTRONIC MATERIALS LETTERS Vol.17 No.1

Dielectric constants of polymer/conductor composites are often high, owing to strong interface interaction in those composites. They can be used for dielectric energy storage, but they usually have high dielectric loss and conductivity. In order toreduce the dielectric loss and conductivity in poly(vinylidene fluoride-hexafluoropropylene) (P(VDF-HFP))/V2AlC MAXcomposites, in this study, we explored the novel P(VDF-HFP)/V2AlC MAX/montmorillonite (MMT) ternary composites. Compared with binary composites, the ternary composites showed the mildly reduced dielectric constant, significantlydecreased dielectric loss and conductivity. Using highly-conductive V2AlCMAX filler aimed at taking advantage of thepolymer/MAX interface polarization to increase the dielectric response of composites. Employing well-insulating MMTfiller aimed at reducing the interface electric leakage conduction. At 1 kHz, the outstanding ternary composite with 2 wt%MMT and 20 wt% MAX could exhibit a high dielectric constant of ca. 27 and low dielectric loss of ca. 0.21. This workmight offer a new research idea for the construction of high-performance composite dielectric films containing modernMAX ceramic fillers.

Successful Treatment of Psychiatric Symptoms after Traumatic Brain Injury Using Deep Brain Stimulation to the Anterior Limb of Internal Capsule-Nucleus Accumbens

Bin Zhou,Weiping Kuang,Hongxing Huang,Yong Zhu,Xiaofeng Chen,Liang Li,Ping Yang 대한정신약물학회 2020 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.18 No.4

Psychiatric symptoms are common after traumatic brain injury (TBI), and some patients have poor drug therapeutic efficacy. We report a successfully treated case of psychiatric symptoms after TBI using deep brain stimulation (DBS) to the anterior limb of internal capsule (ALIC)-nucleus accumbens (NAc) in a 76-year-old woman. The patient suffered from auditory hallucination, mood changes, and insomnia caused by TBI. Psychological test assessment showed the scores of Hamilton Anxiety Scale, Hamilton Depression Scale and Positive and Negative Syndrome Scale were 30, 35, and 96 respectively. Head magnetic resonance imaging scan showed right temporal lobe encephalomalacia. Head magnetic resonance spectroscopy (MRS) showed bilateral basal ganglia choline increased relatively. After DBS to the ALIC-NAc, the target parameters were adjusted. The psychiatric symptoms were completely improved and the result of head MRS was normal in the end. The current report declares that DBS is reversible, adjustable and safe in the treatment of psychiatric symptoms caused by TBI. DBS to the ALIC-NAc should be considered as a possible treatment choice once a patient showed psychiatric symptoms after TBI.

Electromagnetic Environment of Transmission Line Based on Full Parameter Online Estimation

Zidan Sun,Xiaofeng Zhou,Likai Liang,Yang Mo 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.2

The parameters of transmission lines have an influence on the electromagnetic environment surrounding theline. This paper proposes a method based on phasor measurement unit (PMU) and supervisory control and dataacquisition (SCADA) to achieve online estimation of transmission line full parameters, such as resistance,reactance and susceptance. The proposed full parameter estimation method is compared with the traditionalmethod of estimating resistance independently based on SCADA system. Then, the electromagnetic environmentis analyzed based on the different parameter estimation methods. The example results illustrate that onlineestimation of transmission line full parameters is more accurate in the analysis of electromagnetic environment,which further confirms its necessity and significance in engineering application.

Realizing Rationally-Balanced Dielectric Properties in Fluoropolymer/Cr2AlC MAX Composites Modified by 2D-BN

Qin Li,Junquan Zhou,Yanhui Chen,Xiaofeng Xia,Maolin Bo,Qihuang Deng,Yefeng Feng 한국고분자학회 2020 Macromolecular Research Vol.28 No.13

Polymer/conductor composite dielectric materials have a high dielectric constant. In recent years, composite dielectrics have become a study hotspot in highdensity energy storage capacitors. However, high dielectric loss and high electrical conductivity make the application of the composite materials very limited. In order to obtain composite materials with high dielectric constant, low dielectric loss and low conductivity, we added the insulating boron nitride (BN) filler into polymer/Cr2AlC composites. As a conductive filler, Cr2AlC ternary-layered-compound (MAX) ceramic has an interface polarization with the polymer matrix, thereby increasing the dielectric constant of the composites. The insulating BN has a very low electrical conductivity, which can effectively reduce the electrical conductivity and dielectric loss of the composites. Compared with the binary polymer/Cr2AlC composite materials, the ternary polymer/Cr2AlC/BN composite materials can achieve the low dielectric loss and low conductivity as well as properly-lowered dielectric constant. The ternary composite material filled with 10 wt% Cr2AlC and 2 wt% BN has a dielectric constant of about 30, a dielectric loss of about 0.7 and a conductivity of about 4.6×10- 7 S m-1 at 100 Hz. In this work, high-performance composite dielectrics were prepared through good cooperation between conductive and insulating ceramic fillers, which might provide an idea for design of promising energy storage capacitors.