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        Degradation and Drug-release Behavior of Polylactic Acid (PLA) Medical Suture Coating with Tea Polyphenol (TP) -Polycaprolactone (PCL)/Polyglycolide (PGA)

        Shuqiang Liu,Gaihong Wu,Xiaofang Zhang,Juanjuan Yu,Mingfang Liu,Yao Zhang,Peng Wang,Xiaolong Yin 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

        The drug-release time of suture should meet with the healing time of wound, so it is needed to control the drugrelease performance of polylactic acid (PLA) suture. In this paper, two biodegradable polymers with different degradation rates, such as polycaprolactone (PCL) and polyglycolide (PGA), were applied to carry the drug of tea polyphenol (TP). The drug-loading finishing solution which is made of PCL/PGA carriers and TP, was coated on the PLA suture. The drug-release rate and time of PLA suture could be regulated by adjusting the proportion of PCL/PGA carriers. The results indicate that thesurface of drug-loaded PLA suture becomes rough. There is no obvious chemical reaction among the drug, carriers and PLA suture, just physical adhesion. With the increasing of PCL in drug-carriers, the strength of suture gradually increases. At 70/30 of PCL/PGA, the fracture elongation of suture reaches the highest point. In process of degradation, the surface of drugloaded PLA suture appears some holes after 25 weeks. The strength of sutures decreases gradually during degradation, and the effective strength time of sutures with various proportions of PCL/PGA is different. The drug-release rate of the suture isfast at early stage and slow at later stage and the higher the PGA proportion is, the faster the drug-release rate of the suture is.

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      • KCI등재

        Effects of Exogenous Organic Acids on the Growth and Antioxidant System of Cosmos bipinnatus under Cadmium Stress

        Jiani Hu,Xinyi Gao,Yajie Yue,Xiaoyu Ming,Linjie Yue,Lijuan Yang,Xue Xiao,Xiaofang Yu 한국원예학회 2022 원예과학기술지 Vol.40 No.5

        Cadmium pollution in China’s urban green spaces is extremely severe, causing a significant increase in the risk of urban environmental pollution. Applying organic acids under Cd stress conditions improves the activity of the major antioxidant enzymes in the AsA-GSH cycle signi- ficantly. In this study, Cosmos bipinnatus seedlings were cultured in a nutritive medium containing 40 µmol·L-1 Cd. They were subsequently supplemented with different concentrations of citric acid and malic acid (0, 0.5, 1.0, 2.0 mmol L-1 ) to analyze the roles of the organic acids in regulating the AsA-GSH cycle involved in plant resistance to Cd stress. Exogenous organic acids inhibited the growth of C. bipinnatus in a dose-dependent manner. AsA, GSH, and GR in the leaves decreased dramatically at high malic acid concentrations (2 mol L-1 ). The contents of GSH and GR and the APX activities showed a downward trend when the seedlings were treated with 0.5–2 mmol·L-1 of citric acid. However, AsA exhibited contrasting results under these conditions. Notably, both the antioxidant and enzyme activities were reduced considerably to a minimum when the citric acid concentration was 2 mmol·L-1 . Low concentrations of exogenous acids increased the activities of APX and GR in the leaves of C. bipinnatus, thus increasing the contents of ASA and GSH. Such increases boost the efficiency of the ASA-GSH cycle in relieving the toxic effect of cadmium on C. bipinnatus. Cadmium pollution in China’s urban green spaces is extremely severe, causing a significant increase in the risk of urban environmental pollution. Applying organic acids under Cd stress conditions improves the activity of the major antioxidant enzymes in the AsA-GSH cycle signi- ficantly. In this study, Cosmos bipinnatus seedlings were cultured in a nutritive medium containing -1 40 µmol·L Cd. They were subsequently supplemented with different concentrations of citric acid -1 and malic acid (0,0.5,1.0,2.0 mmol L ) to analyze the roles of the organic acids in regulating the AsA-GSH cycle involved in plant resistance to Cd stress. Exogenous organic acids inhibited the growth of C. bipinnatus in a dose-dependent manner. AsA, GSH, and GR in the leaves decreased -1 dramatically at high malic acid concentrations (2 mol L ). The contents of GSH and GR and the -1 APX activities showed a downward trend when the seedlings were treated with 0.5–2 mmol·L of citric acid. However, AsA exhibited contrasting results under these conditions. Notably, both the antioxidant and enzyme activities were reduced considerably to a minimum when the citric acid -1 concentration was 2 mmol·L . Low concentrations of exogenous acids increased the activities of APX and GR in the leaves of C. bipinnatus, thus increasing the contents of ASA and GSH. Such increases boost the efficiency of the ASA-GSH cycle in relieving the toxic effect of cadmium on C. bipinnatus.

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        Calcium signalling mediated the regulation of growth and polysaccharide accumulation by light quality in Dendrobium offi cinale protocorms

        Ting Lei,Suping Gao,Xiao Lin,Kaihui Zhang,Yingqi Liu,Wenji Li,Di Hu,Xiaofang Yu,Mingyan Jiang,Qibing Chen,Ying Sun 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2

        This study selected Dendrobium offi cinale protocorms as the experimental material and used diff erent light-quality treatmentsand calcium signalling inhibitors to explore the mechanism of the biological response of calcium to light quality. The results indicate that a 1:3 red:blue (R:B) light ratio promotes the accumulation of biomass and polysaccharides in theprotocorm. Nevertheless, this eff ect can be entirely counteracted by calmodulin (CaM) inhibitors and calcium ion-chelatingagents. The possible mechanisms of this eff ect are as follows: (1) The Ca 2+ -CaM signal-regulated photosynthetic apparatusis improved by the R:B 1:3 light, increasing the CaM content and Ca 2+ -ATPase activity. Thus, R:B 1:3 light increases thedevelopment of the photosynthetic apparatus in the D. offi cinale protocorm, enhances its photosynthetic productivity, andpromotes the activities of sucrose phosphatase; accordingly, biomass and sucrose accumulation increase. (2) R:B 1:3 lightenhances Ca 2+ -CaM signalling and regulates glycometabolic processes to promote the activities of sucrose synthase, thusincreasing fructose and glucose levels and providing suffi cient synthetic precursors to promote polysaccharide production.

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