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      • 개조개 근육 및 내장 중의 식품성분의 계절적 변화 1.일반 성분 및 유리 아미노산 조성의 변화

        김귀식,나재경 여수대학교 산업기술지역개발연구소 2001 産業基術硏究所 論文集 Vol.10 No.-

        In order to obtain basic data on the effective utilization of purplish washington clam, it was divided into muscle and viscera. And then changes in proximate composition and free amino acid were investigated. Moisture conentets of muscle and viscera were in the range of 73.2∼83.6%, 76.2∼82.8% and high contents was September 1998 on the other hand low contents was January1999. Crude protein contents of muscle and viscera were in the range of 8.5∼17.3%, 8.8∼16.8% and the both was high contents in the November 1998 and low contents in the January 1999. Crude fat contents of muscle and viscera were in the range of 0.4 ∼1.4%, 1.1 ∼2.4% and viscera was the higher contents than muscle. The major free amino acid of muscle and viscera were aspartic acid, threonine, glutamic acid, glycine, alanine anti lysine. Glycine contents was more higher than other free amino acids. Changes of free ammino acrid of purplish washington clam during period were high contents in the winter and low in the summer, repectively.

      • KCI등재

        사상 체질별로 분류한 여대생의 식사내용이 혈청과 뇨중 성분치에 미치는 영향에 대한 연구

        박귀선,한진숙,김현주 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.3

        This study was conducted to compare the serum composition and urinary excretion in four institutional groups and the effects of intakes of good foods and harmful foods according to the Sasang constitution for a short time on serum and urine composition. The contitutions of 33 female students were classified by O-ring test and muscular strength test according to the Sasang constitution. The number of each institutional group was So-yang-in 11, So-em-in 10 and Tae-em-in 12. No subject belonged to Tae-yang-in. There were no significant differences among contitutional groups in the values of most serum composition and urinary excretion. Serum levels of total cholesterol, glucose, calcium and phosphorus in Tae-em-in was the highest among groups and the level of albumin and total protein in So-em-in was highest. The values of serum compositions changed after the 33 subjects took good foods and then harmful foods according to The Sasang constitution for a short time(5 days). Dietary behavior of the subjects had some effects among the constitutional groups in the values of most serum composition and urinary excretion. But the effects of dietary behavior on the serum composition and urinary excretion showed various trends in the four institutional groups.

      • 家兎眼 망막 색소상피세포 Acid phosphatase : 정제 및 활성에 미치는 인자들에 관한 연구 Purification and Characterization

        석귀덕,송지영 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.5 No.-

        Acid phosphatase [Orthophosphoric monoester phosphohydrolase(acid optimum), EC 3.1.3.2, ACPase] was purified from the retinal pigment epithelium of rabbit eyes near homogeneity. The purification procedure included lysosomal fractionation, acid treatment, ammonium sulfate fractionation, gel filtration chromatography on sephadex G-100 and ion exchange chromatography on DEAE-cellulose. ACPase was purified about 47 fold and it is mollecular weight was estimated to be approximately 126 kDa, and consists of two identical subunits(54 kDa) by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimum temperature of ACPase activity determined with p-nitirophenyl phosphate was 60℃. ACPase was inhibited by L-(+)-tartarate, fluoride, cupric ion, zinc ion and mercuric ion, whereas activated by magnesium ion, reducing agents(cystein, 2-mercaptoethanol or ascorbic acid) and EDTA. Although the enzyme appears to be the most activity toward P-nitrophenyl phosphate, marked activity was observed with Glucose-1-phosphate. ATP, ADP and AMP were not hydrolyzed by ACPase.

      • 지방채의 효율적 운영방안

        김귀곤 全北行政學會 2000 全北行政學報 Vol.14 No.2

        지방자치가 실시 이후 지역주민의 행정수요에 대한 수요가 급격하게 증가할 것으로 예상되고 있으나 각 지방자치단체는 지방재정의 부족으로 인하여 그 부응에 한계를 느끼고 있으며 또한 주민들의 복지욕구와 지역개발수요릉 지방정부 스스로 원할하게 충족시키기도 어려운 실정이다. 이러한 여건 하에서 단기적으로 실현가능성이 높은 정책수단으로서 지방채에 대한 수요와 관심이 증가하고 있으나 경직적인 현행 지방체제도와 지방채시장의 취약한 하부구조로 인하여 지방채가 지방자치단체의 신축적인 자원조달수단으로서 역할을 못하고 있을 뿐만 아니라 관리·통제도 효율적으로 이루어지지 못하고 있다. 그러므로 지방채를 활성화함으로써 지방재정수요의 변화에 신축적으로 대응하기 위해서는 지방채의 관련제도를 정비하고 보다 효율적이고 엄격한 관리 및 감독을 통하여 재정부실을 미연에 방지할 수 있는 장치를 마련하는 것이 필요하다. 이를 위한 방안으로는 먼저 제도적 측면에서 기채승인제도의 변화, 지방채 발행계획의 합리화, 지방채관련 전담부서설치와 전문인력의 양성, 지방채 관리능력 및 생산성의 제고, 공모주의를 통한 지방채시장의 확대, 공적기금 또는 금융기구의 설치, 증권발행채의 합리화 등이 이루어져야 하며, 운용적 측면에서 지방채발행규모의 상향조정, 지방채 수요기반 확충, 지방채 유통시장기반의 확충, 해외자본의 활용방안 등이 고려되어야 할 것이다.

      • 독일어축소접미사 -lein과 -chen의 형태변천에 관한 연구

        이귀경 숙명여자대학교 독일어권연구센터 1998 독일문화 Vol.5 No.-

        Die verliegende Abhandlung beabsichtigt zu untersuchen, woher die beide Diminutivsuffixe -lein und -chen in der deutschen Schriftsprache stammen, und wie sie historisch bis heute ihre morphologischen Formen total oder partiel vera¨ndert bzw. behalten haben. Die Entstehung des zweiten Elemente auβser den anlautenden Konsonanten der Diminutivsuffixe d. h. -ein zu -lein und -en zu -chen liegt in dem urspru¨nglichen germainishen Stoff-, Abstammungs-, oder Zugeho¨rigkeitsadjektivsuffix -in-a, das sich in dem gotishen. Zeitaum allma¨hlich zum norminalen Neutrum als Diminutivem versendet. Das Element dem nhd. -lein entsprechende ahd. -ili^n setzt sich aus dem ahd. -il und dem obengenannten germ. -in-a zusammen. Dann bleibt die Frage, woraus ahd. -il- kommt. Die Herkunft des ahd. -il- wird von der zwei Seiten betrachtet. 1. Das demselben entsprechende idg. Diminutivum -?? -l ist besonders in Lateinisch voll belegt. 2. Die zweisilbige Wurzel auf -il mit dem Diminutivsuffix germ. -in-a zu ahd. -in zum Beispiel ahd. fugil -in "Vo¨gelin," ahd. leffil -in "Lo¨ff(e)lein"usw. wird volksetymologisch fu¨r Wurzel Bindvokal Dim. -ane. Dim. -inl. Dim. -ausl. fug- -i- -l- -i- -n leff- gehalten. Das dem nhd, -chen entsprchende as. Element -ikin bildet sich aus dem as. -ik- und dem obenerwa¨hnten germ. -in-a zusammen. Und as. -ik- geht zu germ. -ik-, mit den(das Diminutivbedeutung eingeschlossenen) manigfaltigen Bedeutungen zuru¨ck, dessen idg. Wurzel immer noch nicht ganz klar bleibt. Die nhd. Diminutivsuffixe -lein und -chen sind urspru¨nglich die pleonastische Kontamination der verdoppelten. ◁그림 삽입▷(원문을 참조하세요)

      • Classical Swine Fever (Hog cholera) Virus 약독순화주 (Suri 주)의 gp55 Gene 염기서열 분석

        김귀현,장경수,강경임,이병형,박종현,안수환,전무형 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

        An attenuated classical swine fever virus (CSFV), Suri strain, is a variant derived from a vaccine virus, LOM strain. This study was performed to elucidate the molecular biologcal properties of CSFV Suri strain, and to obtain the basic data for molecular epidemiological approaches for the disease. The truncated form of gp55 gene without the C-terminal trasmembrane domain, in size of 1,023bp, was amplified by RT-PCR and sequenced by dye terminator cyclic sequencing method, and inserted into BamHI site of pAcGP67B baculovirus vector, establishing a cloned pAcHEG plasmid. By the nucleotide sequences determined, 341 amino acid sequences were predicted. As compard the nucleotide and amino acid sequences of gp55 of Suri with the various CSFV, Suri strain showed the high homology over 99.1% with ALD and LOM strains, but comparably the lower homology with Alfort and Brescia. In comparison of amino acid sequence in variable domain of gp55 protein, the similar tendency of homology was observed. In hydrophobicity analysis, all of four CSFV strains revealed the analogous patterns of hydrophobicity. The numbers and locations of N-glycosylation site and cysteine residuse in gp55 were analyzed, those of Suri strain being coincident with ALD and LOM strains. The results suggest that gp55 in Suri strain has the high similarity to those in ALD and LOM strains in terms of the nucleotide and amino acid sequences and the functional properties of gp55 protein.

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