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Xiaowei Ding,Kaihui Liu,Yonggui Zhang,Feihu Liu 한국미생물학회 2017 The journal of microbiology Vol.55 No.11
In the present study, we identified genes that are putatively involved in the production of fungal 10-hydroxycamptothecin via transcriptome sequencing and characterization of the Xylaria sp. M71 treated with salicylic acid (SA). A total of 60,664,200 raw reads were assembled into 26,044 unigenes. BLAST assigned 8,767 (33.7%) and 10,840 (41.6%) unigenes to 40 Gene Ontology (GO) annotations and 108 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, respectively. A total of 3,713 unigenes comprising 1,504 upregulated and 2,209 downregulated unigenes were found to be differentially expressed between SA-induced and control fungi. Based on the camptothecin biosynthesis pathway in plants, 13 functional genes of Xylaria sp. M71 were mapped to the mevalonate (MVA) pathway, suggesting that the fungal 10-hydroxycamptothecin is produced via the MVA pathway. In summary, analysis of the Xylaria sp. M71 transcriptome allowed the identification of unigenes that are putatively involved in 10-hydroxycamptothecin biosynthesis in fungi.
An Enhanced Biometric-Based Three Factors User Authentication Scheme for Multi-server Environments
Youping Lin,Kaihui Wang,Baocan Zhang,Yuzhen Liu,Xiong Li 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.1
Authentication is an important and basic security service for many network based applications, which allows the registered user access remote services after the validity of his/her identity is verified by the remote server. Password, smart card and biometric are three frequently used factors in authentication, and some remote user authentication schemes for different environments had been presented based on these factors by researchers. Recently, Baruah et al. pointed out the weaknesses of Mishra et al.’s three factors user authentication scheme for multi-server environments, and they proposed an enhanced scheme. They claimed that their scheme has many security features and can resist some common attacks. However, based on our analysis, Baruah et al.’s scheme cannot resist stolen smart card attack, cannot protect user’s anonymity, and it is also vulnerable to Denial of Service attack. In this paper, an enhanced three factors user authentication scheme for multi-server environments based on fuzzy extractor technology is proposed, and the analysis show that the proposed scheme is more security and efficient than other related schemes.
Ting Lei,Suping Gao,Xiao Lin,Kaihui Zhang,Yingqi Liu,Wenji Li,Di Hu,Xiaofang Yu,Mingyan Jiang,Qibing Chen,Ying Sun 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2
This study selected Dendrobium offi cinale protocorms as the experimental material and used diff erent light-quality treatmentsand calcium signalling inhibitors to explore the mechanism of the biological response of calcium to light quality. The results indicate that a 1:3 red:blue (R:B) light ratio promotes the accumulation of biomass and polysaccharides in theprotocorm. Nevertheless, this eff ect can be entirely counteracted by calmodulin (CaM) inhibitors and calcium ion-chelatingagents. The possible mechanisms of this eff ect are as follows: (1) The Ca 2+ -CaM signal-regulated photosynthetic apparatusis improved by the R:B 1:3 light, increasing the CaM content and Ca 2+ -ATPase activity. Thus, R:B 1:3 light increases thedevelopment of the photosynthetic apparatus in the D. offi cinale protocorm, enhances its photosynthetic productivity, andpromotes the activities of sucrose phosphatase; accordingly, biomass and sucrose accumulation increase. (2) R:B 1:3 lightenhances Ca 2+ -CaM signalling and regulates glycometabolic processes to promote the activities of sucrose synthase, thusincreasing fructose and glucose levels and providing suffi cient synthetic precursors to promote polysaccharide production.
( Wensong Jin ),( Hui Lin ),( Huifang Gao ),( Zewang Guo ),( Jiahuan Li ),( Quanming Xu ),( Shujing Sun ),( Kaihui Hu ),( Jung-kul Lee ),( Liaoyuan Zhang ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.4
N-acyl-homoserine lactone quorum sensing (AHL-QS) has been shown to regulate many physiological behaviors in Serratia marcescens MG1. In the current study, the effects of AHL-QS on the biosynthesis of acid and neutral products by S. marcescens MG1 and its isogenic ΔswrI with or without supplementing exogenous N-hexanoyl-L-homoserine lactone (C<sub>6</sub>-HSL) were systematically investigated. The results showed that swrI disruption resulted in rapid pH drops from 7.0 to 4.8, which could be restored to wild type by supplementing C<sub>6</sub>-HSL. Furthermore, fermentation product analysis indicated that ΔswrI could lead to obvious accumulation for acidogenesis products such as lactic acid and succinic acid, especially excess acetic acid (2.27 g/l) produced at the early stage of fermentation, whereas solventogenesis products by ΔswrI appeared to noticeably decrease by an approximate 30% for acetoin during 32-48 h and by an approximate 20% for 2,3-butanediol during 24-40 h, when compared to those by wild type. Interestingly, the excess acetic acid produced could be removed in an AHL-QS-independent manner. Subsequently, quantitative real-time PCR was used to determine the mRNA expression levels of genes responsible for acidogenesis and solventogenesis and showed consistent results with those of product synthesis. Finally, by close examination of promoter regions of the analyzed genes, four putative luxI box-like motifs were found upstream of genes encoding acetyl-CoA synthase, lactate dehydrogenase, α-acetolactate decarboxylase, and Lys-like regulator. The information from this study provides a novel insight into the roles played by AHL-QS in switching from acidogenesis to solventogenesis in S. marcescens MG1.