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Xiaolin Yao,Siyi Pan,Chunhong Duan,Fang Yang,Gang Fan,Xinrong Zhu,Shuzhen Yang,Xiaoyun Xu 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.5
Polymethoxylated flavones (PMFs) extracted from Citrus sinensis ‘Jincheng’ peel were characterized by chromatographic and spectroscopic techniques. Seven individual PMF were identified. 3, 3’, 4’, 5, 6, 7-hexamethoxyflavone (HEX), nobiletin (NOB), heptamethoxyflavone (HEP), 5-demethylnobiletin (DN), and tangeretin (TAN) were characterized through electrospray ionization mass spectrometry (ESI-MS) in positive mode of protonated molecular ions [M+H]?, the diagnostic fragment ions, together with the UV-Vis spectra and high performance liquid chromatography (HPLC) elution order from literature data. Sinensetin (SIN) and tetramethyl-O-scutellarein (SCU) were isolated and identified through their MS, ¹H nuclear magnetic resonance (NMR) and UV-Vis spectral studies. The levels of PMFs in peels from different cultivars of citrus fruits grown in China were determined for the first time. The results showed that C. aurantium ‘Bitter orange’ peel was the most promising variety for HEP. C. sinensis peel was a good source for SIN and SCU.
Yang Yao,Chengqiang Ding,Jun Gao,Datong Wu,Xiaolin Liu,Yong Qin,Yong Kong 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.9
Morphologies of different samples.
Volatile Compounds of Orange Wines Produced with and without Peel Contact
Gang Fan,Xiaolin Yao,Yongxia Xu,Huanhuan Li,Hongfei Fu,Kexing Wang,Siyi Pan 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.6
The present study focused on the effects of peel contact on the volatile compounds of orange wines. The volatile compounds were analyzed by sensory and instrumental analyses. Solid-phase microextraction (SPME) was used for extraction of volatile compounds. A total of 19 and 27 volatile compounds were identified in without and with peel contact wines respectively. Esters were quantitatively the dominant group of volatile compounds in without peel contact wines, while terpenes were the most abundant compounds in peel contact wines. Totally 11 and 14 new formed compounds were found in without and with peel contact wines, mainly were esters, alcohols, and acids. According to sensory analysis, the peel contact wine showed a more citrus-like and fruity aroma than the wines without peel contact.
Qi Wang,Xiaolin Fang,Baofei Sun,Kai Zhu,Maolin Yao,Shaofeng Wei,Aihua Zhang 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.4
Arsenic can cause immune inflammation, which is the basis of arsenic-induced damage to multiple organs and systems. Forkhead box P3 (Foxp3)-labelled CD4+CD25+ regulatory T cells (Tregs) play an essential role in maintaining immune homeostasis. Nuclear factor-κb (NF-κB) and Interleukin-2 (IL-2) are critical regulators of Foxp3. Rosa roxburghii Tratt (RRT) is an edible medicinal plant with anti-inflammation effects. In this study, a control group (n = 41) and an arseniasis group (n = 209) were recruited, and screened subjects from the arseniasis patients for RRTJ (n = 46) or placebo (n = 43) to explore the possible mechanism by which RRT alleviates immune inflammation. The results indicated that RRTJ can inhibits NF-κB and increases IL-2, and alleviates the Foxp3-mediated Tregs imbalance in the peripheral blood of arseniasis patients. In summary, these findings suggest a novel intervention or therapeutic target for immune inflammation in arseniasis patients and provide new evidence that RRTJ inhibits immune inflammation.
Yue Li,WanWan Li,Minhua Fang,XiaoLin Yao,Chao Chen,Miao Shui,Jie Shu,Yuanlong Ren 한국물리학회 2017 Current Applied Physics Vol.17 No.8
Zn1-xCoxO (0 x 0.15) anode material was prepared by an easy polyacrylamide assisted sol-gel route. The successful replacement of Zinc by Cobalt within Cobalt content x 0.09 was confirmed by structural characterization. The introduction of Cobalt element greatly improved the electro-chemical performances of the matrix Zinc oxide. Without carbon coating, at the 20th cycle, Zn0.91Co0.09O anode still preserved a capacity a little bit more than 1000 mA h g1 and a capacity more than 600 mA h g1 was retained at the end of the 50th cycle. Better rate capability was also witnessed. The SEM, EIS at OCV, CV and in situ XRD were further carried out to elucidate the lithiation mechanism. The role Cobalt doping played can be summarized as follows: the stabilization of the Li2Zn phase, the minimization of charge transfer resistance and the enhanced reversibility of the reduction from metal oxide to metal.
Liang, Guijie,Xu, Jie,Xu, Weilin,Wang, Luoxin,Shen, Xiaolin,Yao, Mu Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7
The geometries, electronic structures and absorption spectra of the two organic triphenylamine-based dyes TA-St-CA and TA-DM-CA, containing identical electron donors and acceptors but the different conjugated bridges, were studied by density functional theory (DFT) at the B3LYP and PBE1PBE levels, respectively. The influence of para-orientating methoxyl units on the electronic structures and light absorption properties of the dyes and the consequent photovoltaic performance of the dye-sensitized solar cells (DSSCs) were investigated in detail. The results indicate that the introduction of the para-orientating methoxyl units into the conjugated bridge induces the increased absorption wavelength as well as the more negative EHOMO corresponding to the bigger driving force $(E_{I^-/I^-_3}-E_{HOMO})$ for dye reduction, which together improve the photovoltaic performance of TA-DM-CA, although there is a decline of the open circuit voltage caused by the more negative $E_{LUMO}$.
Guijie Liang,Jie Xu,Weilin Xu,Luoxin Wang,Xiaolin Shen,Mu Yao 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.7
The geometries, electronic structures and absorption spectra of the two organic triphenylamine-based dyes TASt-CA and TA-DM-CA, containing identical electron donors and acceptors but the different conjugated bridges, were studied by density functional theory (DFT) at the B3LYP and PBE1PBE levels, respectively. The influence of para-orientating methoxyl units on the electronic structures and light absorption properties of the dyes and the consequent photovoltaic performance of the dye-sensitized solar cells (DSSCs) were investigated in detail. The results indicate that the introduction of the para-orientating methoxyl units into the conjugated bridge induces the increased absorption wavelength as well as the more negative EHOMO corresponding to the bigger driving force (E_I^-/_(I3)^-)− EHOMO) for dye reduction, which together improve the photovoltaic performance of TA-DM-CA, although there is a decline of the open circuit voltage caused by the more negative E_(LUMO).
Mudalal Mahmoud,Wang Zhanqi,Mustafa Shockry,Liu Yiping,Wang Yao,Yu Jize,Wang Shengnan,Sun Xiaolin,Zhou Yanmin 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5
Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields. Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields.