LncRNA MEG3 Regulates Imatinib Resistance in Chronic Myeloid Leukemia via Suppressing MicroRNA-21

( Xiangyu Zhou ),( Ping Yuan ),( Qi Liu ),( Zhiqiang Liu ) 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5

Imatinib resistance has become a major clinical problem for chronic myeloid leukemia. The aim of the present study was to investigate the involvement of MEG3, a lncRNA, in imatinib resistance and demonstrate its underlying mechanisms. RNAs were extracted from CML patients` peripheral blood cells and human leukemic K562 cells, and the expression of MEG3 was measured by RT-qPCR. Cell proliferation and cell apoptosis were evaluated. Western blotting was used to measure the protein expression of several multidrug resistant transporters. Luciferase reporter assay was performed to determine the binding between MEG3 and miR-21. Our results showed that MEG3 was significantly decreased in imatinib-resistant CML patients and imatinib-resistant K562 cells. Overexpression of MEG3 in imatinib-resistant K562 cells markedly decreased cell proliferation, increased cell apoptosis, reversed imatinib resistance, and reduced the expression of MRP1, MDR1, and ABCG2. Interestingly, MEG3 binds to miR-21. MEG3 and miR-21 were negatively correlated in CML patients. In addition, miR-21 mimics reversed the phenotype of MEG3-overexpression in imatinib-resistant K562 cells. Taken together, MEG3 is involved in imatinib resistance in CML and possibly contributes to imatinib resistance through regulating miR-21, and subsequent cell proliferation, apoptosis and expression of multidrug resistant transporters.

LncRNA MEG3 Regulates Imatinib Resistance in Chronic Myeloid Leukemia via Suppressing MicroRNA-21

Zhou, Xiangyu,Yuan, Ping,Liu, Qi,Liu, Zhiqiang The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5

Imatinib resistance has become a major clinical problem for chronic myeloid leukemia. The aim of the present study was to investigate the involvement of MEG3, a lncRNA, in imatinib resistance and demonstrate its underlying mechanisms. RNAs were extracted from CML patients' peripheral blood cells and human leukemic K562 cells, and the expression of MEG3 was measured by RT-qPCR. Cell proliferation and cell apoptosis were evaluated. Western blotting was used to measure the protein expression of several multidrug resistant transporters. Luciferase reporter assay was performed to determine the binding between MEG3 and miR-21. Our results showed that MEG3 was significantly decreased in imatinib-resistant CML patients and imatinib-resistant K562 cells. Overexpression of MEG3 in imatinib-resistant K562 cells markedly decreased cell proliferation, increased cell apoptosis, reversed imatinib resistance, and reduced the expression of MRP1, MDR1, and ABCG2. Interestingly, MEG3 binds to miR-21. MEG3 and miR-21 were negatively correlated in CML patients. In addition, miR-21 mimics reversed the phenotype of MEG3-overexpression in imatinib-resistant K562 cells. Taken together, MEG3 is involved in imatinib resistance in CML and possibly contributes to imatinib resistance through regulating miR-21, and subsequent cell proliferation, apoptosis and expression of multidrug resistant transporters.

Downregulation of lysyl oxidase and lysyl oxidase-like protein 2 suppressed the migration and invasion of trophoblasts by activating the TGF-β/collagen athway in preeclampsia

Xiang-Hong Xu,Yuanhui Jia,Xinyao Zhou,Dandan Xie,Xiaojie Huang,Linyan Jia,Qian Zhou,Qingliang Zheng,Xiangyu Zhou,Kai Wang,Li-Ping Jin 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Preeclampsia is a pregnancy-specific disorder that is a major cause of maternal and fetal morbidity and mortality with a prevalence of 6–8% of pregnancies. Although impaired trophoblast invasion in early pregnancy is known to be closely associated with preeclampsia, the underlying mechanisms remain elusive. Here we revealed that lysyl oxidase (LOX) and LOX-like protein 2 (LOXL2) play a critical role in preeclampsia. Our results demonstrated that LOX and LOXL2 expression decreased in preeclamptic placentas. Moreover, knockdown of LOX or LOXL2 suppressed trophoblast cell migration and invasion. Mechanistically, collagen production was induced in LOX- or LOXL2-downregulated trophoblast cells through activation of the TGF-β1/Smad3 pathway. Notably, inhibition of the TGF-β1/Smad3 pathway could rescue the defects caused by LOX or LOXL2 knockdown, thereby underlining the significance of the TGF-β1/ Smad3 pathway downstream of LOX and LOXL2 in trophoblast cells. Additionally, induced collagen production and activated TGF-β1/Smad3 were observed in clinical samples from preeclamptic placentas. Collectively, our study suggests that the downregulation of LOX and LOXL2 leading to reduced trophoblast cell migration and invasion through activation of the TGF-β1/Smad3/collagen pathway is relevant to preeclampsia. Thus, we proposed that LOX, LOXL2, and the TGF-β1/Smad3/collagen pathway can serve as potential markers and targets for clinical diagnosis and therapy for preeclampsia.

Fabrication and Magnetic Properties of A New Fe-based Amorphous Compound Powder Cores

Xiangyue Wang,Deren Li,Caowei Lu,Feng Guo,Zhichao Lu,Shaoxiong Zhou 한국자기학회 2010 한국자기학회 학술연구발표회 논문개요집 Vol.2010 No.12

Fluorinated and Charged Non-woven Polypropylene Electret Fiber Membrane: A Novel Way in Transformer Oil Regeneration

Nianrong Zhou,Xiao He,Yuyang Yan,Xinliang Guo,Linshan Zhang,Yunhua He,Xiangyu Tan,Zhengyun Fang 한국전기전자재료학회 2022 Transactions on Electrical and Electronic Material Vol.23 No.2

The regeneration of transformer oil is a trending topic in the dielectric research field. However, the normal way of regeneration requires high cost and complicate process. In this paper, the non-woven polypropylene (PP) electret films were modifi ed by fluorination and corona method to achieve the regeneration function for transformer oil. The results show that the C-H bonds in polypropylene were replaced by C-F bonds during the fluorination process, resulting in the mean diameter of the fibers being decreased to a value of 1.7 μm. The increased roughness of the fi bers leads to super hydrophobicity. Further research revealed that the insulation properties of aging oil recovered after fi ltration by corona charged fl uorinated fi ber membranes. The result is especially signifi cant for breakdown voltage, the values of which reached half of the original insulating oil. In conclusion, the combination of fl uorinating and corona charging method on PP fi ber membrane shows great potential for insulation oil regeneration.

Quality-Related Process Monitoring Based on a Bayesian Classifier

Hongping Zhou,Xiangyu Kong,Jiayu Luo,Qiusheng An,Hongzeng Li 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.24 No.12

Multivariate statistical analysis approaches are extensively employed in process monitoring because they can effectively detect abnormal conditions in industrial processes. However, both Gaussian and non-Gaussian variables are often present in industrial processes. A single multivariate statistical process monitoring method often has difficulty simultaneously dealing with variable information of mixed distribution characteristics. This paper proposes a multivariate quality-related process monitoring method based on a Bayesian classifier to address this issue. The proposed method separates the variables into Gaussian and non-Gaussian parts using a Jarque–Bera test. Then, Gaussian and non-Gaussian properties are extracted through modified kernel partial least squares and kernel independent component analysis. After feature extraction, a Bayesian-based classifier relevance vector machine is constructed to monitor quality-related information of the process, which avoids the construction of a threshold in conventional methods and offsets the drawbacks of insufficient single statistic information. A numerical simulation and the Tennessee-Eastman process verify the effectiveness of the method.

Fabrication and Magnetic Properties of A New Fe-based Amorphous Compound Powder Cores

Wang Xiangyue,Guo Feng,Lu Caowei,Lu Zhichao,Li Deren,Zhou Shaoxiong 한국자기학회 2011 Journal of Magnetics Vol.16 No.3

A new Fe-based amorphous compound powder was prepared from Fe-Si-B amorphous powder by crushing amorphous ribbons as the first magnetic component and Fe-Cr-Mo metallic glassy powder by water atomization as the second magnetic component. Subsequently by adding organic and inorganic binders to the compound powder and cold pressing, the new Fe-based amorphous compound powder cores were fabricated. This new Fe-based amorphous compound powder cores combine the superior DC-Bias properties and the excellent core loss. The core loss of 500 ㎾/㎥ at Bm = 0.1T and f = 100 ㎑ was obtained When the mass ratio of FeSiB/FeCrMo equals 3:2, and meanwhile the DC-bias properties of the new Fe-based amorphous compound powder cores just decreased by 10% compared with that of the FeSiB powder cores. In addition, with the increasing of the content of the FeCrMo metallic glassy powder, the core loss tends to decrease.

Phenylboronic acid-functionalized gelatin–oleic acid nanoparticles for high loading and efficient transdermal delivery of Celastrol towards the treatment of psoriasis

Jiangtao Su,Na Lin,Xiangyu You,Heshuang Dai,Meng Rao,Lu Ye,Fan Ye,Le Cai,Yuxin Chen,Gao Zhou,Xiaoxia Guo 한국고분자학회 2023 Macromolecular Research Vol.31 No.11

Systemic toxicity, poor aqueous solubility, and poorly cell permeable ability hindered the clinical application of Celastrol. In this study, we aimed to design and synthesize an amphiphilic conjugate to encapsulate Celastrol into micelles to improve its water solubility, cellular membrane penetration, improving the clinic translation potential of Celastrol for the treatment of psoriasis. For this purpose, we first synthesized gelatin and oleic acid conjugate (GOC-1), and then covalently bonded 4-(3-boronophenylamino)-4-oxobutanoic acid (BPOA) with GOC-1 to form a stable GOC-2 conjugate which can self-assemble into micelles in aqueous solution. Celastrol (Cel) was physically encapsulated into the core of GOCs micelles. The dynamic stability, particle size, drug release, zeta potential, drug-loading efficiency, and surface morphology of Cel/loaded GOCs nano-micelles were determined. In addition, cell viability, cellular uptake of Cel/loaded GOC-2, and skin permeation and in vivo anti-psoriasis effect of Cel-loaded GOC-2 were investigated. Our results have shown that Cel/loaded GOC-1 and Cel/loaded GOC-2 have spherical shapes with diameters of around 200–300 nm. Compared to GOC-1, GOC-2 micelles showed higher drug-loading efficiency and excellent permeation ability in vitro. Moreover, Cel/GOC-2 micelles reduced erythema and white scales on the dorsal skin of psoriatic mice. In conclusion, BPOA attached GOC nanoparticles as a Celastrol carrier not only increase its water solubility but also improve drug-loading efficiency and cell permeation ability, exhibiting superior anti-psoriatic effect than the commercially available tacrolimus. Our work is expected to provide a facile approach to prepare nanocarrier for Celastrol to improve the clinic translation potential of Celastrol.

Single and binary adsorption of dyes from aqueous solutions using functionalized microcrystalline cellulose from cotton fiber

Hongjuan Bai,Junhang Chen,Xiangyu Zhou,Chengzhi Hu 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.11

Simultaneous removal of dyes in the effluents of printing and dyeing industries is challenging, and the mechanism of the co-adsorption of dyes is still unclear. In this context, a novel adsorbent based on microcrystalline cellulose from cotton fiber through a simplified chemical modification process was prepared. Methylene blue (MB) and neutral red (NR) were selected to investigate their adsorption/co-adsorption on such functionalized microcrystalline cellulose. The experimental adsorption results indicated that the adsorption quantity of both dyes was similar for the single solute. The kinetics of adsorption processes could be better described with the pseudo-second order models for both single and binary dye solutes. The results of the co-adsorption suggested that the extended Langmuir model could well predict equilibrium. The maximum adsorption capacity of MB and NR for the single systems was 115.2 and 83.2mg/g, respectively. Moreover, an antagonistic effect could be found in the binary dye solute. The obtained results revealed that the co-adsorption of dyes might be driven by hydrogen bonding, - stacking interaction as well as electrostatic interaction.

Research Progress in SiC-Based Ceramic Matrix Composites

Dong, Shaoming,Wang, Zhen,Zhou, Haijun,Kan, Yan-Mei,Zhang, Xiangyu,Ding, Yusheng,Gao, Le,Wu, Bin,Hu, Jianbao The Korean Ceramic Society 2012 한국세라믹학회지 Vol.49 No.4

SiC-based ceramic matrix composites show many advantages over their monolithic ceramic counterparts, which makes them potential candidates for applications in various fields. Depending strongly on the chemical composition and microstructure of the fiber reinforcement, matrix as well as the fiber/matrix interphase in the material, the properties of ceramic matrix composites(CMCs) are highly tailorable. In this paper, the latest progresses in the interphase design, matrix modification and fiber reinforcement decoration of CMCs are reviewed, their effects on the properties of the CMCs are introduced.