LncRNA MEG3 Regulates Imatinib Resistance in Chronic Myeloid Leukemia via Suppressing MicroRNA-21

( Xiangyu Zhou ),( Ping Yuan ),( Qi Liu ),( Zhiqiang Liu ) 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5

Imatinib resistance has become a major clinical problem for chronic myeloid leukemia. The aim of the present study was to investigate the involvement of MEG3, a lncRNA, in imatinib resistance and demonstrate its underlying mechanisms. RNAs were extracted from CML patients` peripheral blood cells and human leukemic K562 cells, and the expression of MEG3 was measured by RT-qPCR. Cell proliferation and cell apoptosis were evaluated. Western blotting was used to measure the protein expression of several multidrug resistant transporters. Luciferase reporter assay was performed to determine the binding between MEG3 and miR-21. Our results showed that MEG3 was significantly decreased in imatinib-resistant CML patients and imatinib-resistant K562 cells. Overexpression of MEG3 in imatinib-resistant K562 cells markedly decreased cell proliferation, increased cell apoptosis, reversed imatinib resistance, and reduced the expression of MRP1, MDR1, and ABCG2. Interestingly, MEG3 binds to miR-21. MEG3 and miR-21 were negatively correlated in CML patients. In addition, miR-21 mimics reversed the phenotype of MEG3-overexpression in imatinib-resistant K562 cells. Taken together, MEG3 is involved in imatinib resistance in CML and possibly contributes to imatinib resistance through regulating miR-21, and subsequent cell proliferation, apoptosis and expression of multidrug resistant transporters.

A GIS-based 3D Slope Stability Analysis Method Based on the Assumed Normal Stress on the Slip Surface

( Xiangyu Liu ),( Mowen Xie ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

A geographic information systems(GIS)-based slope stability analysis method is proposed based on the assumption of normal stress distribution acting on the slip surface. first, a three-dimensional slopestability analysis model based on grid column units is established. then, four equilibrium equations are derived under limit equilibrium condition. the normal stress distribution acting on the slip surface is analysed to construct a reasonable normal stress distribution approximatin function for solving the safety factor. moreover, a GIS-based extension module is developed. the accuracy and feasibility of the module are vefified by three typical cases.

LncRNA MEG3 Regulates Imatinib Resistance in Chronic Myeloid Leukemia via Suppressing MicroRNA-21

Zhou, Xiangyu,Yuan, Ping,Liu, Qi,Liu, Zhiqiang The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5

Imatinib resistance has become a major clinical problem for chronic myeloid leukemia. The aim of the present study was to investigate the involvement of MEG3, a lncRNA, in imatinib resistance and demonstrate its underlying mechanisms. RNAs were extracted from CML patients' peripheral blood cells and human leukemic K562 cells, and the expression of MEG3 was measured by RT-qPCR. Cell proliferation and cell apoptosis were evaluated. Western blotting was used to measure the protein expression of several multidrug resistant transporters. Luciferase reporter assay was performed to determine the binding between MEG3 and miR-21. Our results showed that MEG3 was significantly decreased in imatinib-resistant CML patients and imatinib-resistant K562 cells. Overexpression of MEG3 in imatinib-resistant K562 cells markedly decreased cell proliferation, increased cell apoptosis, reversed imatinib resistance, and reduced the expression of MRP1, MDR1, and ABCG2. Interestingly, MEG3 binds to miR-21. MEG3 and miR-21 were negatively correlated in CML patients. In addition, miR-21 mimics reversed the phenotype of MEG3-overexpression in imatinib-resistant K562 cells. Taken together, MEG3 is involved in imatinib resistance in CML and possibly contributes to imatinib resistance through regulating miR-21, and subsequent cell proliferation, apoptosis and expression of multidrug resistant transporters.

Bond Behavior of Spiral Ribbed Ultra-high Strength Steel Rebar Embedded in Plain and Steel Fiber Reinforced High-Strength Concrete

Xiangyu Li,Jianwei Zhang,Juan Liu,Wanlin Cao 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.10

The bond behavior of spiral ribbed ultra-high strength (SRUHS) steel rebar embedded in plain high strength concrete (HSC) and steel fiber reinforced high strength concrete (SFRHSC) is experimentally studied using pull-out tests. The influencing factors of concrete type, embedded length, cover thickness and stirrup ratio are considered and the bond failure characteristics as well as the whole process of stress-slip behavior are analyzed. The research shows good anchorage ductility between SRUHS steel rebar and HSC. Since SRUHS steel rebars have continuous spiral bite teeth with concrete, the concrete in the rib dales is not easily sheared off, thereby leading to a reliable bond strength. Compared to specimens without steel fibers, steel fiber reinforcement can increase the ultimate bond strength, bond stiffness and residual strength. Meanwhile, it will also reduce the influence of cover thickness, stirrup ratio on the specimens with steel fibers. Finally, a prediction calculation for ultimate bond stress and an analytical model for bond stress-slip relation are proposed, which match well with test results.

An Improved Artificial Bee Colony Algorithm and Its Application

Xiangyu Kong,Sanyang Liu,Zhen Wang 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.6

Mechanism of Synergetic Growth of Flexibility and Strength of Biomimetic Nanocomposite Fibre

Xiangyu Duan,Pan Li,Jingyu Ouyang,Zichen Gao,Jiaxin Liu,Jie Wang,Zhigang Xia,Weilin Xu 한국섬유공학회 2022 Fibers and polymers Vol.23 No.3

Nanocomposites with carbon nanotubes (CNTs) can combine the stiffness and multi-functionality of carbonnanotubes with the advantages of high toughness and processability of polymers giving rise to properties different from thatof general composites. However, when the content of CNTs increases gradually, the flexibility of the composite fibre canreduce. In this paper, we propose a simple method of softening the composite fibre via dimensional helical deformation offibre inner macromolecule bundles to avoid the deterioration of fibre flexibility. The theoretical simulations were conductedto predict proper helical deformations of the single fibre to increase fibre softness, followed by practical softening of thepolyvinylidene difluoride (PVDF)/CNTs composite by tensional twisting of the single fibres. The fibres with and withouttensional twisting were tested by Fourier-transformed infrared spectroscopy, scanning electron microscopy, X-ray diffractionand mechanical drawing. Results showed the reinforcement of the PVDF/multi-walled CNTs composite fibres (tensilestrength enhanced from 4.71 to 5.19 cN/dtex) with an evident softness reduction (initial modulus increased from 16.8 to20.52 cN/dtex) as the CNTs content increased from 0 to 1.5 wt%. After the tensional twisting, the initial modulus of thecomposite fibre was reduced by 62.5 % while the fibre strength remained reinforced because biomimetic helix formationimproved the internal structure deformation ability of the fibre.

Geochemistry of the Early Cretaceous saline lacustrine shales on the Lingshan Island, East China: implications for provenance, tectonic setting, and paleoweathering

Xiangyu Zhang,Lu Liu,Qiang Sun,Yanming Xu,Shoujun Li,Xiaoli Wang,Xuxue Wang,Ying Sun,Wenxia Wang 한국지질과학협의회 2023 Geosciences Journal Vol.27 No.4

Early Cretaceous saline lacustrine black shales in the Fajiaying Formation were deposited under hothouse conditions and have a complex structure and history of sedimentary evolution. In this study, integrated petrographic and geochemical investigations were utilized to determine the provenance, tectonic setting, and paleoweathering conditions of these shales, therefore revealing their depositional history. Shale in the area under investigation has not been subjected to any post-depositional alteration, mechanical sorting, or recycling, hence its geochemistry has not been affected. The chondrite normalized rare earth elements (REE) pattern of shales displayed typical characteristics, including an enrichment in light REE (LREE), a flat pattern for heavy REE (HREE), and a negative europium (Eu) anomaly. Specific trace element ratios suggested that the sediments were primarily derived from felsic source rocks. Furthermore, there was no discernible shift in the sediment source between the Qiancengya and the Laohuzui sections. The discrimination diagrams of major and trace elements used to understand the tectonic history showed that the majority of the source rocks originated from the active continental margin (ACM) and continental island-arc (CIArc). The bivariate plots and low average values of CIW (chemical index of weathering) and CIA (chemical index of alteration) for the examined shale suggested that chemical weathering in the source terrain under arid conditions was extremely weak. Importantly, with the change of climate from dry-hot to warmhumid, an increase in CIA and CIW was observed which indicated a gradual increase in chemical weathering.

Association analysis of polymorphism in the NR6A1 gene with the lumbar vertebrae number traits in sheep

Xiangyu Zhang,Cunyuan Li,Xiaoyue Li,Zhijin Liu,Wei Ni,Yang Cao,Yang Yao,Esenbay Islamov,Junchang Wei,Xiaoxu Hou,Shengwei Hu 한국유전학회 2019 Genes & Genomics Vol.41 No.10

Introduction The vertebral number is an economically significant trait, which is associated with body length and carcass traits. Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1) is a member of the nuclear receptor superfamily and it plays an important role in the early development of embryos. Objectives The NR6A1 gene was considered as an important candidate for influence vertebrae number, while the potential associations between this gene and the number of lumbar vertebrae traits of sheep have not been explored. Methods In this study, we detected the genetic variants of NR6A1 gene and analyzed the associations of the polymorphisms with lumbar number traits in 130 Kazakh sheep. We use single-strand conformation polymorphism (SSCP) technique to detect single nucleotide polymorphism (SNP) of NR6A1 gene, and the association of the genotype and lumbar number variation was analyzed by independent Chi-square test. Results We detect SNP of NR6A1 gene by PCR-SSCP technique, and polymorphisms were only found in the coding region of exon-6 and exon-8 of NR6A1 gene. In order to investigate the connection between the SNP locus and lumbar number traits in sheep, we conducted a Chi-square test for independence for exon-6 and exon-8 of NR6A1 gene, respectively. Association analysis revealed significant associations between the SNP (rs414302710: A >C) in the exon-8 of NR6A1 gene with the number of lumbar vertebrae (P < 0.01). Conclusion Our study indicated that this SNP (rs414302710: A>C) locus of exon-8 of NR6A1 gene in sheep possible influence the number of lumbar vertebrae, which has the potential to be applied in selective breeding of sheep.

Formation Mechanism and Adhesion Evaluation of Debris in Ti–6Al–4V Alloy Turning

Changping Li,Xiangyu Liu,Moran Xu,Jielin Chen,Shujian Li,Pengnan Li,Tae Jo Ko 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.5

In this paper, the formation mechanism of adhered debris on the surface of turned titanium alloy was studied. The adhesion rate of debris was calculated and evaluated using image extraction technology. The effects of cutting parameters and cooling conditions on the debris adhesion rate were also studied. Moreover, the chip temperature and trajectory in the turning process are analyzed using a finite element simulation and a thermal camera. The results show that there are three primary sources of adhered debris: the adhesion of the built-up edge, the micro-chips formed during the cutting process, and the micro-chips generated by the friction between the chip and the machined surface. In addition, the cutting speed is also one of the most significant factors affecting the adhesion of debris. The debris adhesion rate drops significantly as the cutting speed decreases. It was also found that the coolant can effectively reduce the debris adhesion rate, and this effect is more evident at low feed rates.

Deadbeat and Hierarchical Predictive Control with Space-Vector Modulation for Three-Phase Five-Level Nested Neutral Point Piloted Converters

Junjie Li,Xiangyu Chang,Dirui Yang,Yunlong Liu,Jianguo Jiang 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.6

To achieve a fast dynamic response and to solve the multi-objective control problems of the output currents, capacitor voltages and system constraints, this paper proposes a deadbeat and hierarchical predictive control with space-vector modulation (DB-HPC-SVM) for five-level nested neutral point piloted (NNPP) converters. First, deadbeat control (DBC) is adopted to track the reference currents by calculating the deadbeat reference voltage vector (DB-RVV). After that, all of the candidate switching sequences that synthesize the DB-RVV are obtained by using the fast SVM principle. Furthermore, according to the redundancies of the switch combination and switching sequence, a hierarchical model predictive control (MPC) is presented to select the optimal switch combination (OSC) and optimal switching sequence (OSS). The proposed DB-HPC-SVM maintains the advantages of DBC and SVM, such as fast dynamic response, zero steady-state error and fixed switching frequency, and combines the characteristics of MPC, such as multi-objective control and simple inclusion of constraints. Finally, comparative simulation and experimental results of a five-level NNPP converter verify the correctness of the proposed DB-HPC-SVM.