An Adaptive Mean Shift Segmentation of Plankton Images

Wentao Dong,Youngsung Soh,Howard Schultz,Allen Hanson 대한전자공학회 2007 ITC-CSCC :International Technical Conference on Ci Vol.2007 No.7

Research on flexural bearing capacity of cold-formed thin-walled steel and reinforced concrete sandwich composite slabs

Wentao Qiao,Zhiyuan Huang,Xiaoshuo Yan,Dong Wang,Lijun Meng 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.45 No.2

The aim of this paper is to study the mechanical behaviors of the cold-formed thin-walled steel and reinforced concrete sandwich composite slab (CTS&RC-SCS) under vertical loads and to develop the calculation methods of its flexural bearing capacity and section stiffness. Two CTS&RC-SCS specimens were designed and manufactured to carry out the static loading test, and meanwhile, the numerical simulation analyses based on finite element method were implemented. The comparison between experimental results and numerical analysis results shows that the CTS&RC-SCS has good flexural capacity and ductility, and the accuracy and rationality of the numerical simulation analysis are verified. Further, the variable parameter analysis results indicate that neither increasing the concrete strength grade nor increasing the thickness of C-sections can significantly improve the flexural capacity of CTS&RC-SCS. With the increase of the ratio of longitudinal bars and the thickness of the composite slab, the flexural capacity of CTS&RC-SCS will be significantly increased. On the basis of experimental research and numerical analysis above, the calculation formula of the flexural capacity of CTS&RC-SCS was deduced according to the plastic section design theory, and section stiffness calculation formula was proposed according to the theory of transformed section. In terms of the ultimate flexural capacity and mid-span deflection, the calculated values based on the formulas and the experimental values are in good agreement.

Isolation and characterization of tick-borne Roseomonas haemaphysalidis sp. nov. and rodent-borne Roseomonas marmotae sp. nov.

Zhu Wentao,Zhou Juan,Lu Shan,Yang Jing,Lai Xin-He,Jin Dong,Pu Ji,Huang Yuyuan,Liu Liyun,Li Zhenjun,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Four novel Gram-negative, mesophilic, aerobic, motile, and cocci-shaped strains were isolated from tick samples (strains 546T and 573) and respiratory tracts of marmots (strains 1318T and 1311). The 16S rRNA gene sequencing revealed that strains 546T and 573 were 97.8% identical to Roseomonas wenyumeiae Z23T, whereas strains 1311 and 1318T were 98.3% identical to Roseomonas ludipueritiae DSM 14915T. In addition, a 98.0% identity was observed between strains 546T and 1318T. Phylogenetic and phylogenomic analyses revealed that strains 546T and 573 clustered with R. wenyumeiae Z23T, whereas strains 1311 and 1318T grouped with R. ludipueritiae DSM 14915T. The average nucleotide identity between our isolates and members of the genus Roseomonas was below 95%. The genomic G+C content of strains 546T and 1318T was 70.9% and 69.3%, respectively. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) were the major polar lipids, with Q-10 as the predominant respiratory quinone. According to all genotypic, phenotypic, phylogenetic, and phylogenomic analyses, the four strains represent two novel species of the genus Roseomonas, for which the names Roseomonas haemaphysalidis sp. nov. and Roseomonas marmotae sp. nov. are proposed, with 546T (= GDMCC 1.1780T = JCM 34187T) and 1318T (= GDMCC 1.1781T = JCM 34188T) as type strains, respectively.

Steam-Chest Molding of Expanded Polypropylene Foams. 2. Mechanism of Interbead Bonding

Zhai, Wentao,Kim, Young-Wook,Jung, Dong Won,Park, Chul B. American Chemical Society 2011 INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH - Vol.50 No.9

<P>Expanded polypropylene (EPP) bead foams were processed using steam-chest molding. Interbead bonds formed during processing and was characterized by observing both the product surface and the fracture surfaces using scanning electron microscopy (SEM) and by measuring the tensile properties of specimens. It was found that both the degree of interbead bonding and the tensile strength had a direct relationship to the steam pressure/temperature and that high steam temperature enhanced the crystallization behavior of EPP beads during the cooling process. A formation mechanism of interbead bonding during processing was proposed based on the crystal melting behaviors, as discussed in the first article of this series (<name name-style='western'>Zhai</name> et al. Ind. Eng. Chem. Res.<x xml:space='preserve'> </x>2010<x xml:space='preserve'>, </x>49<x xml:space='preserve'>, </x>9822). Possible parameters influencing the formation of interbead bonding, namely, steam temperature/pressure and bead foam expansion ratio, were investigated in this study. The effect of postannealing of the EPP products on the shrinkage and crystallinity of the molded EPP products was also addressed.</P>

Sorption of carbon dioxide by ionic liquid-based sorbents

Bi, Wentao,Zhu, Tao,Park, Dong Wha,Row, Kyung Ho Wiley Blackwell (John Wiley Sons) 2012 Asia-Pacific journal of chemical engineering Vol.7 No.1

Amphiphilic Norbornene-Based Diblock Copolymers Containing Polyhedral Oligomeric Silsesquioxane Prepared by Living Ring Opening Metathesis Polymerization

Park, Su-Dong,Xu, Wentao,Chung, Chan-Hong,Kwon, Young-Hwan The Polymer Society of Korea 2008 Macromolecular Research Vol.16 No.2

We report the successful synthesis of poly(NBECOOH-b-NBEPOSS) copolymers, taking advantage of the sequential, living ring opening metathesis polymerization of NBETMS and NBEPOSS using the $RuCl_2(=CHPh)(PCY_3)_2$/$CH_2Cl_2$/$20^{\circ}C$ system, followed by the hydrolysis of trimethylsilyl groups in poly(NBETMS-b-NBEPOSS) copolymers. The living behavior of ROMP of NBETMS was first investigated using two diagnostic plots, a first order kinetic plot and a $\bar{M}_n$ vs. conversion plot. The plots confirmed that no termination and chain transfer reaction had occurred during polymerization. Poly(NBECOOH-b-NBEPOSS) copolymers were prepared using the sequential monomer addition of NBEPOSS to living poly(NBETMS) chain ends, followed by the hydrolysis of trimethylsilyl groups in the poly(NBETMS-b-NBEPOSS) copolymers. The high structural integrity of poly(NBE-COOH-b-NBEPOSS) copolymers was confirmed by $^1H$-NMR, $^{13}C$-NMR spcctroscopy and GPC.

Nonlinear Quality Indices Based on a Novel Lempel-Ziv Complexity for Assessing Quality of Multi-Lead ECGs Collected in Real Time

Yatao Zhang,Zhenguo Ma,Wentao Dong 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.2

We compared a novel encoding LempelZiv complexity (ELZC) with three common complexity algorithmsi.e., approximate entropy (ApEn), sample entropy (SampEn), and classic LempelZiv complexity (CLZC) soas to determine a satisfied complexity and its corresponding quality indices for assessing quality of multileadelectrocardiogram (ECG). First, we calculated the aforementioned algorithms on six artificial time series inorder to compare their performance in terms of discerning randomness and the inherent irregularity within timeseries. Then, for analyzing sensitivity of the algorithms to content level of different noises within the ECG, weinvestigated their change trend in five artificial synthetic noisy ECGs containing different noises at severalsignal noise ratios. Finally, three quality indices based on the ELZC of the multilead ECG were proposed toassess the quality of 862 real 12lead ECGs from the MIT databases. The results showed the ELZC coulddiscern randomness and the inherent irregularity within six artificial time series, and also reflect content levelof different noises within five artificial synthetic ECGs. The results indicated the AUCs of three quality indicesof the ELZC had statistical significance (>0.500). The ELZC and its corresponding three indices were moresuitable for multilead ECG quality assessment than the other three algorithms.

Association of UDP-galactose-4-epimerase with milk protein concentration in the Chinese Holstein population

Li Cong,Cai Wentao,Liu Shuli,Zhou Chenghao,Cao Mingyue,Yin Hongwei,Sun Dong-Xiao,Zhang Shengli,Loor Juan J. 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: An initial RNA-Sequencing study revealed that UDP-galactose-4-epimerase (GALE) was one of the most promising candidates for milk protein concentration in Chinese Holstein cattle. This enzyme catalyzes the interconversion of UDP-galactose and UDP-glucose, an important step in galactose catabolism. To further validate the genetic effect of GALE on milk protein traits, genetic variations were identified, and genotypes-phenotypes associations were performed. Methods: The entire coding region and the 5’-regulatory region (5’-UTR) of GALE were re-sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed linear animal model with a population encompassing 1,027 Chinese Holstein cows. Results: A total of three variants in GALE were identified, including two novel variants (g.2114 A>G and g.2037 G>A) in the 5’-UTR and one previously reported variant (g.3836 G>C) in an intron. All three single nucleotide polymorphisms (SNPs) were associated with milk yield (p<0.0001), fat yield (p = 0.0006 to <0.0001), protein yield (p = 0.0232 to <0.0001) and protein percentage (p<0.0001), while no significant associations were detected between the SNPs and fat percentage. A strong linkage disequilibrium (D’ = 0.96 to 1.00) was observed among all three SNPs, and a 5 Kb haplotype block involving three main haplotypes with GAG, AGC, and AGG was formed. The results of haplotype association analyses were consistent with the results of single locus association analysis (p<0.0001). The phenotypic variance ratio above 3.00% was observed for milk protein yield that was explained by SNP-g.3836G >C. Conclusion: Overall, our findings provided new insights into the polymorphic variations in bovine GALE gene and their associations with milk protein concentration. The data indicate their potential uses for marker-assisted breeding or genetic selection schemes.

Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs

Zhang Fei,Peng Wuxun,Wang Tao,Zhang Jian,Dong Wentao,Wang Chuan,Xie Zhihong,Luo Hong,Liu Gang 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Bone marrow mesenchymal stem cells (BMSCs) have been used in the treatment of early steroid-induced osteonecrosis of the femoral head (SONFH). However, the hypoxic microenvironment in the osteonecrotic area leads to hypoxia-induced apoptosis of transplanted BMSCs, which limits their efficacy. Therefore, approaches that inhibit hypoxia-induced apoptosis of BMSCs are promising for augmenting the efficacy of BMSC transplantation. Our present study found that under hypoxia, the expression of the long noncoding RNA (Lnc) transmembrane protein 235 (Tmem235) was downregulated, the expression of Bcl-2-associated X protein was upregulated, the expression of B-cell lymphoma-2 protein was downregulated, and the apoptotic rate of BMSCs was over 70%. However, overexpression of Lnc Tmem235 reversed hypoxia-induced apoptosis of BMSCs and promoted their survival. These results demonstrated that Lnc Tmem235 effectively inhibited hypoxia-induced apoptosis of BMSCs. Mechanistically, we found that Lnc Tmem235 exhibited competitive binding to miR-34a-3p compared with BIRC5 mRNA, which is an inhibitor of apoptosis; this competitive binding relieved the silencing effect of miR-34a-3p on BIRC5 mRNA to ultimately inhibit hypoxia-induced apoptosis of BMSCs by promoting the expression of BIRC5. Furthermore, we cocultured BMSCs overexpressing Lnc Tmem235 with xenogeneic antigen-extracted cancellous bone to construct tissue-engineered bone to repair a model of early SONFH in vivo. The results showed that overexpression of Lnc Tmem235 effectively reduced apoptosis of BMSCs in the hypoxic microenvironment of osteonecrosis and improved the effect of BMSC transplantation. Taken together, our findings show that Lnc Tmem235 inhibited hypoxia-induced apoptosis of BMSCs by regulating the miR-34a-3p/BIRC5 axis, thus improving the transplantation efficacy of BMSCs for treating early SONFH.

2019 Novel Coronavirus (COVID-19) Pneumonia: Serial Computed Tomography Findings

Jiangping Wei,Huaxiang Xu,Jingliang Xiong,Qinglin Shen,Bing Fan,Chenglong Ye,Wentao Dong,Fangfang Hu 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.4

From December 2019, Coronavirus disease 2019 (COVID-19) pneumonia (formerly known as the 2019 novel Coronavirus [2019nCoV]) broke out in Wuhan, China. In this study, we present serial CT findings in a 40-year-old female patient with COVID-19 pneumonia who presented with the symptoms of fever, chest tightness, and fatigue. She was diagnosed with COVID-19 infection confirmed by real-time reverse-transcriptase-polymerase chain reaction. CT showed rapidly progressing peripheral consolidations and ground-glass opacities in both lungs. After treatment, the lesions were shown to be almost absorbed leaving the fibrous lesions.