Review : Optimization of Influencing Factors on Biomass Accumulation and 5-Aminolevulinic Acid (ALA) Yield in Rhodobacter sphaeroides Wastewater Treatment

( Shuli Liu ),( Xiangkun Li ),( Guangming Zhang ),( Jie Zhang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.11

This study aimed to optimize four factors affecting biomass accumulation and 5-aminolevulinic acid (ALA) yield together with pollutants removal in Rhodobacter sphaeroides wastewater treatment. Results showed that it was feasible to produce biomass and ALA in R. sphaeroides wastewater treatment. Microaerobic, 1,000-3,000 lux, and pH 7.0 were optimal conditions for the highest ALA yield of 4.5 ± 0.5 mg/g-biomass. Under these conditions, COD removal and biomass production rate were 93.3 ± 0.9% and 31.8 ± 0.5 mg/l/h, respectively. In addition, trace elements Fe2+, Mg2+, Ni2+, and Zn2+ further improved the ALA yield, COD removal, and biomass production rate. Specifically, the highest ALA yield (12.5 ± 0.6 mg/g-biomass) was achieved with Fe2+ addition.

Impact of high-speed rail network development on airport traffic and traffic distribution: Evidence from China and Japan

Liu, Shuli,Wan, Yulai,Ha, Hun-Koo,Yoshida, Yuichiro,Zhang, Anming Elsevier 2019 Transportation research Part A, Policy and practic Vol.127 No.-

<P><B>Abstract</B></P> <P>We explore the impacts of high-speed rail (HSR) development on airport-level traffic by considering not only the availability of air-HSR intermodal linkage between the airport and HSR station but also the position of the airport’s city in the HSR network. The latter is measured by both the degree centrality (to reflect connectivity) and the harmonic centrality (to reflect accessibility). Using a sample of 46 airports in China and a sample of 16 airports in Japan over the period of 2007–2015, we conduct regression analysis and compare the effects of HSR network development on airports in these two Northeast Asian countries. We find that as HSR connectivity or accessibility increases, there is, on average, a decline in airports’ domestic and total traffic in China but little change in Japan. Meanwhile, we observe a strong complementary effect of HSR to feed international flights with the presence of air-HSR intermodal linkage. As a result, some airports may experience a total traffic increase. In China, hub airports tend to gain traffic regardless the availability of air-HSR linkage, while non-hub airports are likely to lose. In Japan, on the other hand, airports with air-HSR linkage tend to gain traffic regardless the hub status. Our analysis also reveals some differentiated impacts of HSR connectivity and accessibility in China. An important policy implication is that the investment in air-HSR intermodal linkage at busy airports may not help with realizing the benefit of congestion mitigation and emission reduction. Rather, policy makers may invest air-HSR linkage at regional airports which have the potential to be converted into international gateway hubs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A network view of HSR development is taken to study its impact on airport traffic. </LI> <LI> HSR connectivity and accessibility affect airport traffic differently. </LI> <LI> Airport-HSR station linkage and airport hub status play substantial roles. </LI> <LI> On average the impacts in China are stronger than in Japan, especially in domestic markets. </LI> <LI> HSR development tends to make airport traffic more concentrated to large hubs in China. </LI> </UL> </P>

Paridis Saponins Inhibiting Carcinoma Growth and Metastasis In Vitro and In Vivo

Shuli Man,Wenyuan Gao,Yanjun Zhang,Chaoyi Ma,Liu Yang,Yiwen Li 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.1

Paris polyphylla Smith var. yunnanensis extracts, Rhizoma Paridis saponins (RPS) have been found to show strong antitumor activity. However, few studies have yet investigated pulmonary metastasis treatment with this herb. To detail the effective components in RPS and discuss the preliminary mechanism of antitumor effects in vivo and in vitro, a mixture isolated from RPS was investigated. The main constituents were identified as polyphyllin D, formosanin C, dioscin, Paris H, Paris VII and pennogennin 3-O-α-L-rhamnopyranosyl (1→4)-[β-L-rhamnopyranosyl (1→2)]-β-D-glucopyranoside. In our experiments, LA795 cells were exposed to the mixed compounds. Migration inhibition was evaluated by wound healing assay and migration assay in non-cytotoxic dose which was determined by MTT assay. The results demonstrated that the constituent in varying degrees inhibited the migration of the tumor cells in vitro. The mixture also showed antitumor effects on carcinoma in vivo. In conclusion, the mixture is a potent anticancer agent that elicits programmed cell death and inhibits the migration in murine lung adenocarcinoma, both in vitro and in vivo.

Polar Transmitter with Differential DSM Phase and Digital PWM Envelope

Zhou, Bo,Liu, Shuli The Institute of Electronics and Information Engin 2014 Journal of semiconductor technology and science Vol.14 No.3

A low-power low-cost polar transmitter for EDGE is designed in $0.18{\mu}m$ CMOS. A differential delta-sigma modulator (DSM) tunes a three-terminal voltage-controlled oscillator (VCO) to perform RF phase modulation, where the VCO tuning curve is digitally pre-compensated for high linearity and the carrier frequency is calibrated by a dual-mode low-power frequency-locked loop (FLL). A digital intermediate-frequency (IF) pulse-width5 modulator (PWM) drives a complementary power-switch followed by an LC filter to achieve envelope modulation with high efficiency. The proposed transmitter with 9mW power dissipation relaxes the time alignment between the phase and envelope modulations, and achieves an error vector magnitude (EVM) of 4% and phase noise of -123dBc/Hz at 400kHz offset frequency.

Polar Transmitter with Differential DSM Phase and Digital PWM Envelope

Bo Zhou,Shuli Liu 대한전자공학회 2014 Journal of semiconductor technology and science Vol.14 No.3

A low-power low-cost polar transmitter for EDGE is designed in 0.18μm CMOS. A differential delta-sigma modulator (DSM) tunes a three-terminal voltage-controlled oscillator (VCO) to perform RF phase modulation, where the VCO tuning curve is digitally pre-compensated for high linearity and the carrier frequency is calibrated by a dual-mode lowpower frequency-locked loop (FLL). A digital intermediate-frequency (IF) pulse-width5 modulator (PWM) drives a complementary power-switch followed by an LC filter to achieve envelope modulation with high efficiency. The proposed transmitter with 9mW power dissipation relaxes the time alignment between the phase and envelope modulations, and achieves an error vector magnitude (EVM) of 4% and phase noise of ?123dBc/Hz at 400kHz offset frequency.

Association of UDP-galactose-4-epimerase with milk protein concentration in the Chinese Holstein population

Li Cong,Cai Wentao,Liu Shuli,Zhou Chenghao,Cao Mingyue,Yin Hongwei,Sun Dong-Xiao,Zhang Shengli,Loor Juan J. 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.11

Objective: An initial RNA-Sequencing study revealed that UDP-galactose-4-epimerase (GALE) was one of the most promising candidates for milk protein concentration in Chinese Holstein cattle. This enzyme catalyzes the interconversion of UDP-galactose and UDP-glucose, an important step in galactose catabolism. To further validate the genetic effect of GALE on milk protein traits, genetic variations were identified, and genotypes-phenotypes associations were performed. Methods: The entire coding region and the 5’-regulatory region (5’-UTR) of GALE were re-sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed linear animal model with a population encompassing 1,027 Chinese Holstein cows. Results: A total of three variants in GALE were identified, including two novel variants (g.2114 A>G and g.2037 G>A) in the 5’-UTR and one previously reported variant (g.3836 G>C) in an intron. All three single nucleotide polymorphisms (SNPs) were associated with milk yield (p<0.0001), fat yield (p = 0.0006 to <0.0001), protein yield (p = 0.0232 to <0.0001) and protein percentage (p<0.0001), while no significant associations were detected between the SNPs and fat percentage. A strong linkage disequilibrium (D’ = 0.96 to 1.00) was observed among all three SNPs, and a 5 Kb haplotype block involving three main haplotypes with GAG, AGC, and AGG was formed. The results of haplotype association analyses were consistent with the results of single locus association analysis (p<0.0001). The phenotypic variance ratio above 3.00% was observed for milk protein yield that was explained by SNP-g.3836G >C. Conclusion: Overall, our findings provided new insights into the polymorphic variations in bovine GALE gene and their associations with milk protein concentration. The data indicate their potential uses for marker-assisted breeding or genetic selection schemes.

An Improved Multi-Rules-Based ACO Algorithm for FJSS Problem in Cloud Manufacturing Environment

Hongguo Zhang,Linyan He,Chao Ma,Shuli Zhang,Shenghui Liu 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.7

In cloud manufacturing environment, for a scheduling Job, there may be a lot of servicizing manufacturing resources and manufacturing capability that can be used to support for its realizing. Therefore, how to efficiently solve FJSS problem becomes more complex and significant. First, this paper uses disjunctive graph model to analyze the characteristic of FJSS problems, and then, focusing on machine selection sub problem, this paper designs multi-rules to solve machine selection conflicts in different scenarios. Finally, on this basis, an improved multi-rules-based ACO algorithm is proposed. The algorithm is applied to the typical examples of the flexible job-shop scheduling problem. Compared with other algorithms, final experimental results indicate that this algorithm is effective.

Correlation Between Different Antidiarrheal Treatments and Changes in Chemical Components of Allii Sativi Bulbus Before and After Steaming Treatment Based on Flora Sequencing and In Vitro Experiments

Yarong Li,Yaqian Zhou,Huanjin Liu,Chenxu Wei,Shuli Chen,Zhengying Hua,Yan Xu,Yu Wu,Weidong Li 한국식품영양과학회 2022 Journal of medicinal food Vol.25 No.10

We investigated the changes in the main active ingredients and pharmacodynamic differences in the therapeutic effect of garlic before and after steaming and the correlation between them. The main active ingredients in raw garlic products (RGPs) and steamed garlic products (SGPs) were determined by high-pressure liquid chromatography and ultraviolet spectroscopy. Acute rapid diarrhea (AD) and antibiotic-induced diarrhea (DD) models were established in rats, and each group was treated with RGP and SGP, respectively. The main chemical components of garlic changed before and after steaming. Garlicin and alliinase were only found in RGP, whereas only alliin was found in SGP. Both RGP and SGP contained garlic polysaccharides. For in vivo experiments on AD, the average rate of loose stools was 100.00 ± 0.00, 31.55 ± 11.76, and 19.14 ± 6.62 in the RGP high-dose and SGP high-dose treatment groups, respectively; in DD, the rates were 91.11 ± 14.40, 19.33 ± 3.63, and 30.56 ± 4.30, respectively (P < .01, treatment vs. model groups). In AD, the average grade of loose stools was 2.33 ± 0.52 and 1.83 ± 0.75 in the model and RGP high-dose treatment groups, respectively (P < .05); in DD, the values were 2.17 ± 0.41 in the model group and 1.67 ± 0.52 in the SGP high-dose treatment group (P < .05). RGP had a better therapeutic effect on AD, mainly related to the antibacterial effect of garlicin in RGP. SGP had a better therapeutic effect on DD, mainly related to the alliin and garlic polysaccharide in SGP. This study could provide evidence to support the clinical use of garlic.

Simultaneous adsorption of Cd2+ and methylene blue from aqueous solution using xanthate-modified baker’s yeast

Mingyao Song,Zhengyang Duan,Ronggao Qin,Xiaojun Xu,Shuli Liu,Shumin Song,Mengjiao Zhang,Yue Li,Jiemei Shi 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.6

Xanthate-modified baker’s yeast (XMBY) was successfully synthesized by grafting xanthate groups onto the surface of baker’s yeast and was used for the simultaneous adsorption of cadmium (Cd2+) and methylene blue (MB) from aqueous solution. Scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) and Fourier transform infrared spectroscopy (FTIR) were used to characterize the synthesized adsorbent. The results indicated that the sulfur groups were successfully introduced onto the surface of the baker’s yeast and participated in the adsorption processes. The kinetic and isotherm data showed good correlations with the pseudo-second-order model and the Langmuir model, respectively. The equilibrium time and the maximum values obtained from the two models were 40 min, 239.8mg/g for Cd2+ and 300 min, 64.45mg/g for MB, respectively. The thermodynamic analysis (G<0, H>0, S>0) demonstrated that the adsorption processes of Cd2+ and MB onto the XMBY were endothermic and spontaneous. In the binary-component solution (Cd2+ and MB), the adsorption capacity for MB was almost unaffected by the presence of Cd2+ and interestingly, the adsorption capacity for Cd2+ increased in the presence of MB. Overall, these results indicated that XMBY could be a promising adsorbent for the treatment of wastewater containing both Cd2+ and MB.