Power Decoupling Method for Single Phase Differential Buck Converter

Wenli Yao,Yi Tang,Xiaobin Zhang,Xiongfei Wang,Poh Chiang Loh,Frede Blaabjerg 전력전자학회 2015 ICPE(ISPE)논문집 Vol.2015 No.6

The well-known inherent second-order ripple power in single phase converters imposes harmonic stress on the dc link, resulting in low efficiency and overheating issues. In order to avoid installing bulky electrolytic capacitors or LC filters in the dc-link, this paper presents a differential buck inverter to improve the dc link power quality, and an improved active power decoupling method is proposed to achieve ripple power reduction for both AC-DC and DC-AC conversions. The ripple energy storage is realized by the filter capacitors, which are connected between the output terminal and the negative dc bus. By properly controlling the differential mode voltage of the capacitors, it is possible to transfer desired energy between the DC port and AC port. The common mode voltage is controlled in such a way that the ripple power on the dc side will be reduced. Furthermore, an autonomous reference generation technique is proposed to provide accurate ripple power compensation, and closed-loop controllers are also designed based on small signal models. The effectiveness of this power decoupling method is verified by detailed simulation studies as well as laboratory prototype experimental results.

A Color-Reaction-Based Biochip Detection Assay for RIF and INH Resistance of Clinical Mycobacterial Specimens

( Wen Fei Xue ),( Jing Fu Peng ),( Xiao Li Yu ),( Shu Lin Zhang ),( Boping Zhou ),( Dan Qing Jiang ),( Jian Bo Chen ),( Bing Bing Ding ),( Bin Zhu ),( Yao Li ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1

The widespread occurrence of drug-resistant Mycobacterium tuberculosis places importance on the detection of TB (tuberculosis) drug susceptibility. Conventional drug susceptibility testing (DST) is a lengthy process. We developed a rapid enzymatic color-reaction-based biochip assay. The process included asymmetric multiplex PCR/templex PCR, biochip hybridization, and an enzymatic color reaction, with specific software for data operating. Templex PCR (tem- PCR) was applied to avoid interference between different primers in conventional multiplex- PCR. We applied this assay to 276 clinical specimens (including 27 sputum, 4 alveolar lavage fluid, 2 pleural effusion, and 243 culture isolate specimens; 40 of the 276 were non-tuberculosis mycobacteria specimens and 236 were M. tuberculosis specimens). The testing process took 4.5 h. A sensitivity of 50 copies per PCR was achieved, while the sensitivity was 500 copies per PCR when tem-PCR was used. Allele sequences could be detected in mixed samples at aproportion of 10%. Detection results showed a concordance rate of 97.46% (230/236) in rifampicin resistance detection (sensitivity 95.40%, specificity 98.66%) and 96.19% (227/236) in isoniazid (sensitivity 93.59%, specificity 97.47%) detection with those of DST assay. Concordance rates of testing results for sputum, alveolar lavage fluid, and pleural effusion specimens were 100%. The assay provides a potential choice for TB diagnosis and treatment.

Research on Recognition of Graphic Symbols in Amusement Park: A Case Study of Taiwan`s Theme Amusement Park

( Yao Wen Hsu ),( Yi Chan Chung ),( Ching Piao Chen ),( Chih Hung Tsai ) 한국품질경영학회 2008 The Asian Journal on Quality Vol.9 No.2

A Study of the Ergonomics Evaluation of a Water Heater`s Case Manufacturing Factory

( Yao Wen Hsu ),( Yi Chan Chung ),( Chung Ching Chiu ),( Ching Piao Chen ),( Chih Hung Tsai ) 한국품질경영학회 2008 The Asian Journal on Quality Vol.9 No.1

Wnt/β-Catenin Promotes the Osteoblastic Potential of BMP9 Through Down-Regulating Cyp26b1 in Mesenchymal Stem Cells

Yao Xin-Tong,Li Pei-pei,Liu Jiang,Yang Yuan-Yuan,Luo Zhen-Ling,Jiang Hai-Tao,He Wen-Ge,Luo Hong-Hong,Deng Yi-Xuan,He Bai-Cheng 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.5

BACKGROUND: All-trans retinoic acid (ATRA) promotes the osteogenic differentiation induced by bone morphogenetic protein 9 (BMP9), but the intrinsic relationship between BMP9 and ATRA keeps unknown. Herein, we investigated the effect of Cyp26b1, a critical enzyme of ATRA degradation, on the BMP9-induced osteogenic differentiation in mesenchymal stem cells (MSCs), and unveiled possible mechanism through which BMP9 regulates the expression of Cyp26b1. METHODS: ATRA content was detected with ELISA and HPLC–MS/MS. PCR, Western blot, and histochemical staining were used to assay the osteogenic markers. Fetal limbs culture, cranial defect repair model, and micro–computed tomographic were used to evaluate the quality of bone formation. IP and ChIP assay were used to explore possible mechanism. RESULTS: We found that the protein level of Cyp26b1 was increased with age, whereas the ATRA content decreased. The osteogenic markers induced by BMP9 were increased by inhibiting or silencing Cyp26b1 but reduced by exogenous Cyp26b1. The BMP9-induced bone formation was enhanced by inhibiting Cyp26b1. The cranial defect repair was promoted by BMP9, which was strengthened by silencing Cyp26b1 and reduced by exogenous Cyp26b1. Mechanically, Cyp26b1 was reduced by BMP9, which was enhanced by activating Wnt/b-catenin, and reduced by inhibiting this pathway. b-catenin interacts with Smad1/5/9, and both were recruited at the promoter of Cyp26b1. CONCLUSIONS: Our findings suggested the BMP9-induced osteoblastic differentiation was mediated by activating retinoic acid signalling, viadown-regulating Cyp26b1. Meanwhile, Cyp26b1 may be a novel potential therapeutic target for the treatment of bone-related diseases or accelerating bone-tissue engineering.

Assessing Effects of China’s Party Diplomacy vis-a-vis Southeast Asia: Ideas, Interests, and Controversies

Yao Wen 경남대학교 극동문제연구소 2023 ASIAN PERSPECTIVE Vol.47 No.4

China’s party diplomacy—the Communist Party of China’s own international outreach—has become an important component of China’s foreign relations. Given Southeast Asia’s strategic importance, the Communist Party of China (CPC) has been proactively engaging political elites in the region. What are the immediate changes party diplomacy brings about for Southeast Asian actors, and how do they translate into long-term consequences? I propose a threepronged framework to explore the local effects of China’s party diplomacy, respectively, concerning exposure to ideas, the pursuit of interests, and the emergence of controversies. Drawing on a novel dataset and using illustrative cases of Vietnam, Singapore, Myanmar, and Malaysia, I show how the effects are contingent on the agency of Southeast Asian actors and domestic dynamics in each country. From the standpoint of Southeast Asian elites, engagement with the CPC could bring about ideational and material benefits, while domestic pushback, if any, is easy to deflect and tends to fade away over time. China’s party diplomacy is best described as a moderate status quo multiplier that leads to noticeable changes without triggering structural shifts.

Wind tunnel investigation on flutter and buffeting of a three-tower suspension bridge

Wen-ming Zhang,Yao-jun Ge 한국풍공학회 2017 Wind and Structures, An International Journal (WAS Vol.24 No.4

The Maanshan Bridge over Yangtze River in China is a new long-span suspension bridge with double main spans of 2×1080 m and a closed streamline cross-section of single box deck. The flutter and buffeting performances were investigated via wind tunnel tests of a full bridge aeroelastic model at a geometric scale of 1:211. The tests were conducted in both smooth wind and simulated boundary layer wind fields. Emphasis is placed on studying the interference effect of adjacent span via installing a wind deflector and a wind separating board to shelter one span of the bridge model from incoming flow. Issues related to effects of mid-tower stiffness and deck supporting conditions are also discussed. The testing results show that flutter critical wind velocities in smooth flow, with a wind deflector, are remarkably lower than those without. In turbulent wind, torsional and vertical standard deviations for the deck responses at midspan in testing cases without wind deflector are generally less than those at the midspan exposed to wind in testing cases with wind deflector, respectively. When double main spans are exposed to turbulent wind, the existence of either span is a mass damper to the other. Furthermore, both effects of mid-tower stiffness and deck supporting conditions at the middle tower on the flutter and buffeting performances of the Maanshan Bridge are unremarkable.

A novel role of dipeptidyl peptidase 9 in epidermal growth factor signaling.

Yao, Tsun-Wen,Kim, Woo-Shin,Yu, Denise M T,Sharbeen, George,McCaughan, Geoffrey W,Choi, Kang-Yell,Xia, Pu,Gorrell, Mark D American Association for Cancer Research 2011 Molecular cancer research Vol.9 No.7

<P>Dipeptidyl peptidase IV (DPP4), DPP8, DPP9, and fibroblast activation protein (FAP), the four proteases of the DPP4 gene family, have unique peptidase and extra-enzymatic activities that have been implicated in various diseases including cancers. We report here a novel role of DPP9 in regulating cell survival and proliferation through modulating molecular signaling cascades. Akt (protein kinase B) activation was significantly inhibited by human DPP9 overexpression in human hepatoma cells (HepG2 and Huh7) and human embryonic kidney cells (HEK293T), whereas extracellular signal-regulated kinases (ERK1/2) activity was unaffected, revealing a pathway-specific effect. Interestingly, the inhibitory effect of DPP9 on Akt pathway activation was growth factor dependent. DPP9 overexpression caused apoptosis and significantly less epidermal growth factor (EGF)-mediated Akt activation in HepG2 cells. However, such inhibitory effect was not observed in cells stimulated with other growth factors, including connective tissue growth factor, hepatic growth factor, insulin or platelet-derived growth factor-BB. The effect of DPP9 on Akt did not occur when DPP9 enzyme activity was ablated by either mutagenesis or inhibition. The phosphatidylinositol 3-kinase (PI3K)/Akt pathway is a major downstream effector of Ras. We found that DPP9 and DPP8, but not DPP4 or FAP, associate with H-Ras, a key signal molecule of the EGF receptor signaling pathway. These findings suggest an important signaling role of DPP9 in the regulation of survival and proliferation pathways.</P>

Aerostatic instability mode analysis of three-tower suspension bridges via strain energy and dynamic characteristics

Wen-ming Zhang,Kai-rui Qian,Li Wang,Yao-jun Ge 한국풍공학회 2019 Wind and Structures, An International Journal (WAS Vol.29 No.3

Multispan suspension bridges make a good alternative to single-span ones if the crossed strait or river width exceeds 2-3 km. However, multispan three-tower suspension bridges are found to be very sensitive to the wind load due to the lack of effective longitudinal constraint at their central tower. Moreover, at certain critical wind speed values, the aerostatic instability with sharply deteriorating dynamic characteristics may occur with catastrophic consequences. An attempt of an in-depth study on the aerostatic stability mode and damage mechanism of three-tower suspension bridges is made in this paper based on the assessment of strain energy and dynamic characteristics of three particular three-tower suspension bridges in China under different wind speeds and their further integration into the aerostatic stability analysis. The results obtained on the three bridges under study strongly suggest that their aerostatic instability mode is controlled by the coupled action of the anti-symmetric torsion and vertical bending of the two main-spans’ deck, together with the longitudinal bending of the towers, which can be regarded as the first-order torsion vibration mode coupled with the first-order vertical bending vibration mode. The growth rates of the torsional and vertical bending strain energy of the deck after the aerostatic instability are higher than those of the lateral bending. The bending and torsion frequencies decrease rapidly when the wind speed approaches the critical value, while the frequencies of the anti-symmetric vibration modes drop more sharply than those of the symmetric ones. The obtained dependences between the critical wind speed, strain energy, and dynamic characteristics of the bridge components under the aerostatic instability modes are considered instrumental in strength and integrity calculation of three-tower suspension bridges.

Storage strategies for containers in container yards

Yao Wen Ma,Su Min Jeon,Kap Hwan Kim 대한산업공학회 2008 대한산업공학회 춘계학술대회논문집 Vol.2008 No.5

Container storage problem is a critical issue for the efficient operation of container terminal. The storage yard in the study is horizontal layout, in which one quay crane is served by several yard cranes and one vessel is served by several blocks during vessel operation. This study addresses various storage strategies for horizontal container lay yard layout. The storage strategies include the strategy for selecting blocks, reserving spaces for a group of containers in advance, and selecting yard stacks. The proposed strategies are tested by a simulation study.