ACTIVE LQR MULTI-AXLE-STEERING METHOD FOR IMPROVING MANEUVERABILITY AND STABILITY OF MULTI-TRAILER ARTICULATED HEAVY VEHICLES

You-Qun Zhao,Zhao Wen Deng,Qi Xian Zhao,Bao Hua Wang,Wei Gao,Xin Xin Kong 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.4

Directional performance and highway stability are two important aspects that need to be considered in development and design of a heavy articulated vehicles. To improve the maneuverability and stability of a multi-trailer articulated heavy vehicle (MTAHV), an active linear quadratic regulator (LQR) multi-axle-steering method is designed and examined. First, a linear yaw-plane model with four-degree-of freedom (4-DOF) for MTAHV is built and validated. Thus, a reference model supplying the desired state responses is introduced. Then, an active control algorithm of multi-axle-steering for the rear axles of tractor and full-trailer is investigated, and a LQR controller is proposed based on the linear vehicle model to make the control variables track the desired state responses. The control strategy concentrates on keeping the actual yaw rate and side-slip angle follow the steady-state yaw rate and zero side-slip angle. Finally, the effectiveness of the designed approach on enhancing the maneuverability and stability of the MTAHV have been validated through the simulations of the low-speed 360o roundabout and the single lane-change maneuver with high speed, respectively. The method has a certain reference value for improving the active safety of the MTAHV.

Effects of Parafibromin Expression on the Phenotypes and Relevant Mechanisms in the DLD-1 Colon Carcinoma Cell Line

Zhao, Shuang,Sun, Hong-Zhi,Zhu, Shi-Tu,Lu, Hang,Niu, Zhe-Feng,Guo, Wen-Feng,Takano, Yasuo,Zheng, Hua-Chuan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Background: Parafibromin is a protein encoded by the HRPT2 (hyperparathyroidism 2) oncosuppressor gene and its down-regulated expression is involved in pathogenesis of parathyroid, breast, gastric and colorectal carcinomas. This study aimed to clarify the effects of parafibromin expression on the phenotypes and relevant mechanisms of DLD-1 colon carcinoma cells. Methods: DLD-1 cells transfected with a parafibromin-expressing plasmid were subjected to examination of phenotype, including proliferation, differentiation, apoptosis, migration and invasion. Phenotype-related proteins were measured by Western blot. Parafibromin and ki-67 expression was detected by immunohistochemistry on tissue microarrays. Results: The transfectants showed higher proliferation by CCK-8, better differentiation by electron microscopy and ALP activity and more apoptotic resistance to cisplatin by DNA fragmentation than controls. There was no difference in early apoptosis by annexin V, capase-3 activity, migration and invasion between DLD-1 cells and their transfectants. Ectopic parafibromin expression resulted in down-regulated expression of smad4, MEKK, GRP94, GRP78, $GSK3{\beta}$-ser9, and Caspase-9. However, no difference was detectable in caspase-12 and -8 expression. A positive relationship was noted between parafibromin and ki-67 expression in colorectal carcinoma. Conclusions: Parafibromin overexpression could promote cell proliferation, apoptotic resistance, and differentiation of DLD-1 cells.

Ginsenoside Rh2 differentially Mediates microRNA Expression to Prevent Chemoresistance of Breast Cancer

Wen, Xu,Zhang, He-Da,Zhao, Li,Yao, Yu-Feng,Zhao, Jian-Hua,Tang, Jin-Hai Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Chemoresistance is the most common cause of chemotherapy failure during breast cancer (BCA) treatment. It is generally known that the mechanisms of chemoresistance in tumors involve multiple genes and multiple signaling pathways,; if appropriate drugs are used to regulate the mechanisms at the gene level, it should be possible to effectively reverse chemoresistance in BCA cells. It has been confirmed that chemoresistance in BCA cells could be reversed by ginsenoside Rh2 (G-Rh2). Preliminary studies of our group identified some drugresistance specific miRNA. Accordingly, we proposed that G-Rh2 could mediate drug-resistance specific miRNA and corresponding target genes through the gene regulatory network; this could cut off the drug-resistance process in tumors and enhance treatment effects. G-Rh2 and breast cancer cells were used in our study. Through pharmaceutical interventions, we could explore how G-Rh2 could inhibit chemotherapy resistance in BCA, and analyze its impact on related miRNA and target genes. Finally, we will reveal the anti-resistance molecular mechanisms of G-Rh2 from a different angle in miRNA-mediated chemoresistance signals among cells.

Ram semen preserved at 0℃ with soybean lecithin Tris-based extender substituted for egg yolk

Zhao, Jian-qing,Xiao, Guo-liang,Zhu, Wen-liang,Fang, Di,Li, Na,Han, Chun-mei,Gao, Qing-hua Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.2

Objective: The present study evaluated the preservation of ram semen at 0℃ using soybean lecithin with a Tris-fructose extender. Methods: Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×108 sperm/mL, followed by cooling to 0℃ in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288 h, respectively. Two hundred and twenty-three ewes were inseminated for 216 h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination. Results: The results showed that there were no differences in sperm progressive motility at 0, 24, 72, and 144 h (p>0.05). After 216 h, the sperm progressive motility in the control group and 0.5% concentration groups was significantly higher when compared to 0.25% concentration (p<0.05). The 0.5% concentration group demonstrated the highest survival rate and had no difference with the control group (p>0.05). At 216 h, the sperm progressive motility of all groups was still above 50%. The acrosome integrity of all groups was decreased with prolongation of storage time, but there was no difference at each time point (p>0.05). There was no significant difference in the lambing rate and pregnancy rate between the 0.5% concentration group and the control group (p>0.05). Conclusion: These results suggest that ram sperm is capable of fertilization after preservation at 0℃ with 0.5% of soybean lecithin in Tris-based extender substituted for egg yolk and produce normal offspring after insemination.

Kocuria subflava sp. nov., isolated from marine sediment from the Indian Ocean

Jiang, Zhao,Zhang, Wei-Hua,Yuan, Chang-Guo,Chen, Jia-Yang,Cao, Li-Xiang,Park, Dong-Jin,Xiao, Min,Kim, Chang-Jin,Li, Wen-Jun Springer-Verlag 2015 Antonie van Leeuwenhoek Vol.108 No.6

벼 이앙재배에서 유기질 복합비료 시용이 잡초억제와 벼 생육환경에 미치는 영향

Xue-Hua An,Xue-Ping Zhao,이상복(Sang-Bok Lee),임일빈(Il-Bin Im),Wen-Hao An 韓國雜草學會 2010 Weed&Turfgrass Science Vol.30 No.3

본 연구는 유기질 복합비료가 벼 재배 논에서 잡초 및 벼 생육에 미치는 영향을 파악하기 위하여 수행되었다. 실내 검정 시험에서 유기질 복합비료를 125-250㎏ 10a-1 처리시 이앙벼는 지상부가 10.3~27.2% 증가하였고, 피는 50.3-89.2%의 출현이 억제되었으며, 사마귀풀은 낮은 처리량에서도 높은 감수성을 보였다, 유기질 복합비료는 피, 물달개비, 사마귀풀에 대하여 담수심(1-5㎝)이 깊을수록 높게 억제하였으나, 올방개와 같은 사초과 잡초에 대해서는 억제 효과가 나타나지 않았다. 또 20-35℃의 비교적 높은 온도에서 피와 물달개비는 각각 75.4~92.2%, 49.5~81.6%의 높은 출현억제율을 나타냈다. 유기질 복합비료 시용 10일 후 낙수 시 피와 물달개비는 각각 33.7%, 23.0%의 출현억제율을 나타내었다. 포장시험에서 시험 후 토양의 유기물은 관행구애서 유기질 복합비료 시용구보다 약간 낮게 나타났다. 벼의 생육은 초장 및 경수가 관행구 대비 적었으며 그 차이는 생육후기에 더 많이 나타났고, 벼의 수량은 관행구 대비 4% 감소하였다. 논잡초 억제 효과는 유기질 복합비료 시용구에서 60.5%로 관행구 보다 낮게 나타났다. The effects of organic-compound fertilizer on weed control and rice growth in the transplanting rice cultivation were researched in this article. The results showed that the growth of transplanted rice was promoted (the increased rate was 10.3 - 27.2 %), but the plant number for Echinochloa crus-galli was controlled (the inhibition rate was 50.3~89.2%), besides, small amount of organic-compound fertilizer in controlling Aneilema keisak was highly effective when the transplanted rice was treated with 125~250 ㎏ 10a-1 organic-compound fertilizer. Moreover, E. crus-galli, Monochoria vaginalis and A. keisak were inhibited largely by increasing the water depth (1~5 ㎝), whereas Eleocharis kuroguwai and other sedges weeds were not effected by water depth. Compared with the temperature of 20~35℃, the plant occurrence of E. crus-galli and M. vaginalis at high temperature was inhibited (the inhibition rate were 75.4 -92.2% and 49.5% - 81.6%, respectively). When the transplanted rice was treated with the above organic-compound fertilizer after 10day, the inhibition rate of plant occurrence for E. crus-galli and M. vaginalis was 33.7% and 23.3%, respectively. Similarly, in the later period of rice growth, the plant height and the numbers of tiller grew slowly in conventional soil in comparison with the soil treated with organic-compound fertilizer. It has been found that the rice yield in experimental soil was decreased by 4%. This result in the field experiment showed that organic material contents in conventional soil were lower than that in soil which used the organic-compound fertilizer. Weed control in experimental soil showed 60.5% lower effects than that in conventional soil.

Apoptotic Effect of Propyl Gallate in Activated Rat Hepatic Stellate Cells

Xian-Hua Che,손동환,Wen-Yi Jiang,Daya Ram Parajuli,Yu-Zhe Zhao,이성희 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12

Hepatic stellate cells (HSCs) play a central role in liver fibrosis. Inhibition of HSC growth and induction of apoptosis have been proposed as therapeutic strategies for the treatment and prevention of liver fibrosis. Propyl gallate (PG) is an antioxidant widely used in processed foods, cosmetics and medicinal preparations. However, the anti-fibrotic effect of PG in liver injury is unclear. In this study, we investigated whether PG could induce apoptosis in activated HSCs. Treatment of activated HSCs with PG inhibited cell viability in a dose- and time-dependent manner. PG induced apoptosis as demonstrated by morphological changes, poly(ADP-ribose) polymerase (PARP) cleavage, caspase-3 cleavage, increased Bad expression, and decreased Bcl-2 protein expression. Through stimulation of the activation of c-Jun NH2-terminal protein kinase (JNK) and p38 mitogen-activated protein kinases (MAPK) by PG treatment, we demonstrated that JNK and p38 MPAK are not involved in PG-induced apoptosis using their specific inhibitors. Taken together, these findings indicate that PG induces apoptosis in activated HSCs. The potential anti-fibrotic effect of PG warrants further evaluation.

Micrococcus endophyticus sp. nov., isolated from surface-sterilized Aquilaria sinensis roots.

Chen, Hua-Hong,Zhao, Guo-Zhen,Park, Dong-Jin,Zhang, Yu-Qin,Xu, Li-Hua,Lee, Jae-Chan,Kim, Chang-Jin,Li, Wen-Jun Society for General Microbiology 2009 International journal of systematic and evolutiona Vol.59 No.5

<P>A Gram-positive bacterial strain, designated YIM 56238(T), was isolated from plant roots (Aquilaria sinensis), and characterized by using a polyphasic approach. Strain YIM 56238(T) grew optimally at pH 7.0-8.0 and at 28 degrees C. Analysis of the 16S rRNA gene sequence of strain YIM 56238(T) indicated that it belongs to the genus Micrococcus. Chemotaxonomic data strongly supported the classification of this strain within the genus Micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine and glycine; the predominant menaquinones were MK-8(H(2)) (63.6 %) and MK-7(H(2)) (21.1 %); the phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown ninhydrin-negative phospholipid; and the major cellular fatty acids were iso-C(15 : 0) (30.95 %) and anteiso-C(15 : 0) (53.75 %). The G+C content of the genomic DNA was 72.9 mol%. A number of physiological features were found that clearly distinguished strain YIM 56238(T) from recognized species of the genus Micrococcus. DNA-DNA hybridization studies suggested that the novel strain represents a separate genomic species. On the basis of the data, therefore, strain YIM 56238(T) represents a novel species of the genus Micrococcus, for which the name Micrococcus endophyticus sp. nov. is proposed. The type strain is YIM 56238(T) (=DSM 17945(T)=KCTC 19156(T)).</P>

Luminescence and photoconductivity properties of porous polycrystalline silicon

Yue Zhao,Dong-sheng Li,Jie Zhao,Wen-bin Sang,De-ren Yang,Min-hua Jiang 한국물리학회 2008 Current Applied Physics Vol.8 No.2

In recent years, the photoluminescence and the photoconductivity of porous silicon were comprehensively studied. But the photoluminescence and the photoconductivity of porous polycrystalline silicon have not been wholely studied. In this paper, the results showed that luminescent property of the samples prepared by poly-crystal silicon wafers may be related to the defects on Si complexes surface, which can be proved by microwave-detected photoconductivity decay measurements. Furthermore, the luminescence of samples was disappeared under the external illumination, which may be related to the elimination of luminescent-centers. In addition, the conductivities of the samples were dependent on etched time and current density, and the large porosity of samples led to isotropic photoconductivity, which may be related to the change of energy band structure of the devices. In recent years, the photoluminescence and the photoconductivity of porous silicon were comprehensively studied. But the photoluminescence and the photoconductivity of porous polycrystalline silicon have not been wholely studied. In this paper, the results showed that luminescent property of the samples prepared by poly-crystal silicon wafers may be related to the defects on Si complexes surface, which can be proved by microwave-detected photoconductivity decay measurements. Furthermore, the luminescence of samples was disappeared under the external illumination, which may be related to the elimination of luminescent-centers. In addition, the conductivities of the samples were dependent on etched time and current density, and the large porosity of samples led to isotropic photoconductivity, which may be related to the change of energy band structure of the devices.

Phase Stability and Thermo-Physical Properties of Nickel-Aluminum Binary Chemically Disordered Systems via First-Principles Study

Zhiqin Wen,Yuhong Zhao,Jianhua Li,Hua Hou 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.6

The effect of Al content and crystal structures on ground state, phase stability, elasticity and thermodynamics of Ni1−xAlx(x = 0.25, 0.50 and 0.75) binary chemically disordered systems are investigated using first-principles method in combinationwith quasi-harmonic Debye-Grüneisen model. The special quasirandom structures are applied to model disordered bodycenteredcubic (bcc) and face-centered cubic (fcc) phases. The Gibbs free energy of mixing of equiatomic Ni0.5Al0.5is thelowest. The nonmagnetic fcc structure’ Ni1−xAlx are predicted to be more favorable phases. Disordered Ni1−xAlx are lessstable than ordered L21Ni3Aland B2 NiAl, and L21phase is the most likely to form a nuclear growth. The somewhat differentimpact of Al content on elastic properties has been extracted that the resistance to volume change, shear deformation andelastic deformation of Ni1−xAlx decrease with increasing Al content. For bcc and fcc phases, Ni0.75Al0.25and Ni0.25Al0.75arepredicted to be ductile behavior, while Ni0.5Al0.5exhibit brittleness. The structural, vibrational and electronic contributionsare taken into account to study the thermodynamic properties at finite temperature. The lattice constants a and volumetricthermal expansion coefficient α of Ni1−xAlx systems increase with the increase of Al content. Nevertheless, it is decreasingfor heat capacity Cv and Cvvib. The vibrational entropy Svib of bcc Ni0.25Al0.75is the largest in considered temperature. The α,Cvvib and Svib of disordered Ni1−xAlx are larger than that of ordered Ni3Aland NiAl. Vibrational and electronic entropy arethe dominating at finite temperature stabilization mechanism.