Characterization of Brain Microstructural Abnormalities in High Myopia Patients: A Preliminary Diffusion Kurtosis Imaging Study

Wang Huihui,Wen Hongwei,Li Jing,Chen Qian,Li Shanshan,Wang Yanling,Wang Zhenchang 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.7

Objective: To evaluate microstructural damage in high myopia (HM) patients using 3T diffusion kurtosis imaging (DKI). Materials and Methods: This prospective study included 30 HM patients and 33 age- and sex-matched healthy controls (HCs) with DKI. Kurtosis parameters including kurtosis fractional anisotropy (FA), mean kurtosis (MK), axial kurtosis (AK), and radial kurtosis (RK) as well as diffusion metrics including FA, mean diffusivity, axial diffusivity (AD), and radial diffusivity derived from DKI were obtained. Group differences in these metrics were compared using tract-based spatial statistics. Partial correlation analysis was used to evaluate correlations between microstructural changes and disease duration. Results: Compared to HCs, HM patients showed significantly reduced AK, RK, MK, and FA and significantly increased AD, predominately in the bilateral corticospinal tract, right inferior longitudinal fasciculus, superior longitudinal fasciculus, inferior fronto-occipital fasciculus, and left thalamus (all p < 0.05, threshold-free cluster enhancement corrected). In addition, DKI-derived kurtosis parameters (AK, RK, and MK) had negative correlations (r = -0.448 to -0.376, all p < 0.05) and diffusion parameter (AD) had positive correlations (r = 0.372 to 0.409, all p < 0.05) with disease duration. Conclusion: HM patients showed microstructural alterations in the brain regions responsible for motor conduction and visionrelated functions. DKI is useful for detecting white matter abnormalities in HM patients, which might be helpful for exploring and monitoring the pathogenesis of the disease.

Anti-collision Device for the One-Dimensional Scanning Probe Fitted to a Measuring Machine

Huihui Tian,Hongxi Wang,Guanwei Wang,Wenhong Liang 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.11

The probe is at the heart of the measuring machine operation. A variety of probe types and designs are available today. However, many users continue to use the conventional one-dimensional scanning ones because of their low cost, adequate level of accuracy and performance. During the measurement process, impact phenomena between the probe ball and the part surface occur frequently due to inaccurate programming of the software and improper measurement operation, as well as the one-dimensional scanning probe is infinitely stiff in the motion direction along the machine axis, resulting in plastic deformation of the probe stylus and damage of the probe sensor. It is therefore desirable to protect the scanning probe against collision with a workpiece which is to be measured. A new anti-collision device incorporating a compliant mechanism and permanent magnets is designed in this paper. The analysis of dynamic performance for anti-collision system regarding contact force, magnetic force, velocity, position, has been performed and the maximum contact force in the event of collision is available. Numerical results and prototype experiments show the validity of the designed anti-collision device for the one-dimensional scanning probe.

KAT8/MOF-Mediated Anti-Cancer Mechanism of Gemcitabine in Human Bladder Cancer Cells

( Huihui Zhu ),( Yong Wang ),( Tao Wei ),( Xiaoming Zhao ),( Fuqiang Li ),( Yana Li ),( Fei Wang ),( Yong Cai ),( Jingji Jin ) 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.2

Histone acetylation is a well-characterized epigenetic modification controlled by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Imbalanced histone acetylation has been observed in many primary cancers. Therefore, efforts have been made to find drugs or small molecules such as HDAC inhibitors that can revert acetylation levels to normal in cancer cells. We observed dose-dependent reduction in the endogenous and exogenous protein expression levels of KAT8 (also known as human MOF), a member of the MYST family of HATs, and its corresponding histone acetylation at H4K5, H4K8, and H4K16 in chemotherapy drug gemcitabine (GEM)-exposed T24 bladder cancer (BLCA) cells. Interestingly, the reduction in MOF and histone H4 acetylation was inversely proportional to GEM-induced γH2AX, an indicator of chemotherapy drug effectiveness. Furthermore, pGL4-MOF-Luc reporter activities were significantly inhibited by GEM, thereby suggesting that GEM utilizes an MOF-mediated anti-BLCA mechanism of action. In the CCK-8, wound healing assays and Transwell® experiments, the additive effects on cell proliferation and migration were observed in the presence of exogenous MOF and GEM. In addition, the promoted cell sensitivity to GEM by exogenous MOF in BLCA cells was confirmed using an Annexin V-FITC/PI assay. Taken together, our results provide the theoretical basis for elucidating the anti-BLCA mechanism of GEM.

Image Color and Texture-based Modeling and Simulation of Ripeness Identification for Fresh Corn Ears

Huihui Wang,Yan Lv,Yonghai Sun,Xuejun Wang,Xueheng Tao,Jixin Yang 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.6

Fabrication of Protective Textile with N-doped TiO2 Embedded Citral Microcapsule Coating and Its Air Purification Properties

Shuo Wang,Huihui Ding,Yishi Zhao,Yonggui Li,Wei Wang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.2

The protective textile was successfully prepared using N-doped TiO2 (N-TiO2) embedded citral microcapsule coating. N-TiO2 loaded citral microcapsules were synthesized through interfacial polymerization. Results showed the average diameter of N-TiO2 loaded citral microcapsule with core/shell (Mcitral : MHDI) 2:1 was 1.069 μm. Its encapsulation efficiency was 75.63 %. The citral content from finished cotton fabric measured by gas chromatography was up to 2.90 mg/g (weight of essence: weight of fabric). The essence release content of citral was nearly 50 % of their aroma after 12 weeks, exhibiting good physical function for air purification. Additionally, aromatic textile coated with N-TiO2 embedded citral microcapsules also exhibited photocatalytic activity for the degradation of formaldehyde under visible light irradiation. This functionalfabric would have potential applications in the air purification filed due to the chemical and physical dual effect.

Expression profiles of genes involved in fatty acid and lipid biosynthesis in developing seeds of Paeonia ostii

Li Chaoqiong,Hu Lizong,Que Beibei,Hu Yueran,Guo Yuanyuan,Zhang Minghui,Wang Zenan,Wang Xueqin,Liu Hongzhan,Wang Junsheng,Tian Huihui,Li Xiaoli 한국유전학회 2021 Genes & Genomics Vol.43 No.8

Background Paeonia ostii seeds were identifed as novel sources of edible plant oil with a high proportion of α-linolenic acid, a type of n-3 fatty acid with many health benefts. Due to the unreliability of seed oil content and quality, it is necessary to discover the mechanism underlying lipid biosynthesis in Paeonia ostii seeds. Objectives This study aimed to identify the key genes involved in lipid biosynthesis in Paeonia ostii seeds by analyzing the relationship among the seed characteristics and the expression patterns of lipid genes in Paeonia ostii during seed development. Methods Preliminary research on Paeonia ostii seed development was carried out from 10 days after pollination until maturity, focusing on phenology, oil content and lipid profles. In addition, we investigated the spatiotemporal expression of 36 lipid biosynthetic genes in Paeonia ostii by using quantitative real-time PCR. Results The results suggested that the development of Paeonia ostii seeds from pollination to maturity could be divided into three periods. The 36 lipid genes showed various spatiotemporal expression patterns and fve gene groups with distinct temporal patterns during seed development were identifed by clustering analysis of expression data. Furthermore, the relationships between gene expression and lipid/fatty acid accumulation and some candidate key lipid genes were discussed. Conclusions This study provided the global patterns of fatty acid and lipid biosynthesis-related gene expression, which are critical to understanding the molecular basis of lipid biosynthesis and identifying the lipid accumulation rate-limiting genes during seed development.

Quantitative characterization and magnetic separation of copper pyrometallurgical tailing for molybdenum and copper pre-concentration and cleaning of environmentally hazardous elements

Huihui Zhou,Guijian Liu,Chuncai Zhou,Yu Chen,Muhammad Arif,Mei Sun,Yuan Liu,Hongyang Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.122 No.-

The comprehensive and harmless utilization of copper slag flotation tailing (CSFT) is the key to a wastefreeand sustainable copper industry. Here, the mineralogy and molybdenum micro-dissemination inCSFT were quantified and characterized, and the feasibility of the comprehensive recovery of Mo, Cuand Fe resources from CSFT and hazardous elements fractionation behavior by magnetic separationwas discussed. To investigate the occurrence and abundance of Mo in each phase, CSFT was classified intofour types of phases, including magnetite/hematite (27.26%), silicate associations (43.37%), and metallicsulphides and oxides. Molybdenum distribution is closely related to magnetite/hematite-Fe and S. Themagnetic separation results indicated that Mo and Cu were enhanced in magnetic products by 34–41and 15–21%, respectively. Cleaner non-magnetic residues were found to decrease significantly by 37–44, 58–60, and 11–19% for Cu, As, and Cr, respectively. Mineral fractionation was observed instead ofchemical changes during magnetic separation. Despite a weak separation effect on magnetic and nonmagneticphases due to their close bonds and fine-disseminated minerals, the Fe-silicate associationswithout magnetic phases were well separated into non-magnetic residues. The enrichment of Ca, Mgand separation of an iron-silicate component in non-magnetic residues enhanced the cementitious propertyand allowed the development of more pathways of reutilization.

High-Throughput Sequencing Reveals Bacterial Diversity in Raw Milk Production Environment and Production Chain in Tangshan City of China

Huihui Cao,Yanhua Yan,Lei Wang,Lixue Dong,Xueliang Pang,Sining Tang,Aijun Li,Aili Xiang,Litian Zhang,Baiqin Zheng 한국축산식품학회 2021 한국축산식품학회지 Vol.41 No.3

Raw milk is a nature media of microbiota that access milk from various sources, which constitutes a challenge in dairy production. This study characterizes the relationship between the raw milk quality and the bacteria diversity at different sampling sites in dairy farms, aiming to provide a strong scientific basis for good hygienic practices and optimized procedure in milk production. High-throughput sequencing of 16S rRNA V3-V4 region was used to analyze the components, abundance and diversity of 48 bacterial population sampled from 8 different sites in dairy farm: pre-sterilized cow’s teats (C1), post-sterilized cow’s teats (C2), milking cluster (E), milk in storage tank (M1), transport vehicle (M2), storage equipment (E2), cow’s dung samples (F) and drinking water (W). Firmicutes account for predominantly 32.36% (C1), 44.62% (C2), 44.71% (E), 41.10% (M1), 45.08% (M2), 53.38% (F) of all annotated phyla. Proteobacteria accounts for 81.79% in W group and Actinobacteria 56.43% in E2 group. At the genus level, Acinetobacter was the most abundant genus that causes bovine mastitis, Acinetobacter and Arthrobacter were dominant in C1, C2, and E groups, Kocuria in E2 group and Arcobacter in W group. E, C1, and C2 group have very similar bacterial composition, and M1 and M2 demonstrated similar composition, indicating that the milking cluster was polluted by the environment or contact with cow udders. Bacterial population composition in different sampling sites identified by NGS reveals a correlation between the bacteria communities of raw milk production chain and the quality of raw milk.

Effect of Lateral Boundary Scheme on the Simulation of Tropical Cyclone Track in Regional Climate Model RegCM3

Xiaodan Wang,Zhong Zhong,Yijia Hu,Huihui Yuan 한국기상학회 2010 Asia-Pacific Journal of Atmospheric Sciences Vol.46 No.2

The effect of the lateral boundary scheme in regional climate model (RCM) on the track simulation of tropical cyclone (TC) was investigated using RegCM3, for the case of Winnie (1997), which formed in the Western Pacific and landed on China in August 1997. The results show that there is an inevitable simulation error in the track of Winnie, and the narrower buffer zone size (BZS) will make a great error. However, it was demonstrated that a much broader BZS does not allow a better track simulation of Winnie, and the optimal BZS does not reduce the track error substantially. Moreover, the configuration scheme of nudging parameters plays an important role in the track simulation, and different nudging parameter configuration scheme could make the root mean square errors (RMSEs) of simulated track by more than two times. Nevertheless, the optimal configuration scheme can reduce the track error effectively by maintaining the equilibrium between the two additional nudging terms in the prognostic equations in the buffer zone, whereas both the strong nudging scheme and the weak nudging scheme distort the track simulation of the Winnie. It is also found that the simulated weaker west Pacific subtropical high (WPSH), which leads to the turning of the TC ahead of time, is the reason for the track simulation error. A possible approach for reducing track simulation error of TCs is also discussed.

Research on the Error Detection Technology of Complex Parts based on Improved Bee Colony Algorithm

Jianghua Ge,Huihui Zhang,Yaping Wang,Xiulin Sui,Yongguo Sun 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.12

For Parts of complex structures with multiple characteristics, traditional methods are often difficult and inefficient, so the three-dimensional detection techniques have been developed. But the detection used for the complex structural components still get some problems, such as : it’s difficult to collect data of complex structural components in the data collection process, because of size is always limited ; features of parts retained difficultly in data processing; there are many iterations and calculations in the optimization algorithm of geometric error evaluation, and the search direction is blind. In this paper, the improved colony algorithm is used to get the cylindrical error by establishing the mathematical model with the point cloud data that has been processed. First, collect data acquisition of more structural parts with hand-held laser scanners; Secondly, denoise and streamline the raw point cloud collected before, and then make registration and alignment with the actual CAD model of part; Finally, according to the resultant point cloud data above, in accordance with the principle of minimum condition assessment, establish evaluation model of cylinder error based on improved bee colony algorithm. Experimental results show that for various structural parts, the precision of this detection method is high enough, and detecting speed is also fast.