헤테로폴리산 담지 메조포러스 물질에서 아이소프로필 나프탈렌의 알킬화반응

김명완,김왕기,김종호,서곤 전남대학교 촉매연구소 2000 觸媒硏究 論文集 Vol.21 No.-

The alkylation of 2-isopropyl naphthalene with isopropyl alcohol was studied over heteropoly acid catalyst supported on mesoporous material. The physicochemical state of loaded heteropoly acid was investigated using XRD, nitrogen adsorption and FT-IR techniques. Heteropoly acid was highly dispersed on the wall of mesoporous material, and retained its Br□nsted acidity. The initial conversion of 2-isopropyl naphthalene was high over the heteropoly acid catalyst supported on mesoporous material, while that was negligible over bulk heteropoly acid. The high selectivity for β,β - diisopropyl naphthalene was explained by the uniform mesopore inducing transition-state shape selectivity.

정상 성인에서 수면박탈이 수면구조와 피로감에 미치는 영향

김완중,신석철,왕성근 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

To identify the effects of sleep deprivation on sleep structure polysomnography was performed for 10healthy young adults. Visual analogue scale was also used to identify the effects of sleep deprivation on fatigue, mood, and sleepiness. The subjects were young adults, ranging in age from 21 to 26 years, without past or present histories of significant medical, neurological, or psychiatric illness as well as no current major sleep disturbances or parasomnias. After one adaptation night, each subject was recorded polysomnography in the sleep laboratory. Sleep records were analyzed according to the criteria of Rechtschaffen and Kales every 20 seconds, and tried paired-t-test and correlation. The results were summarized as follows. 1. After one night of total sleep deprivation, compared with the baseline, TST, stage 3, stage 4, and SWS were significanlty increased, and sleep latency was significantly shortened, and return to normal level after 3 nights of recovery. 2. The relative value(%) of stage 3, stage 4, and SWS were positive correlation between the baseline and the first recovery night. 3. Compared with the baseline, reaction time was significantly delayed in the first recovery night, and returned to normal level after 3 days of recovery. 4. The feeling of fatigue, mood, and sleepiness were significantly worsened in the first day after TSD, and returned to normal level after 3 days of recovery.

수생식물을 이용한 수질정화 SYSTEM 개발에 관한 연구

신대윤,홍완해,왕승호 조선대학교 환경공해연구소 1999 環境公害硏究 Vol.15 No.2

This study focused on the removal of COD, SS, T-N and T-P of home wastewater in batch and continuous culture reactors using parsley. And the amounts of nitrogen and phosphorus in the tissues of parsley were also detected. The results are as follow ; 1. Parsley has achieved 42% COD removal rate, 55% SS, 10% T-P and 41% T-N for 48 hours retention time. Residual concentration was COD 6.1㎎/ℓ, SS ll㎎/ℓ, T-P 1.112㎎/ℓ and T-N 4.9㎎/ℓ respectively. 2. Nitrogen and phosphorous content, accumulated in the tissues of parsley are 40.4% and 9.2% respectively in batch reactor with 48 hours SRT. They are presenting increasing rate 37.6% of nitrogen and 9% of phosphorous, compared with their influent sample, contending 2.8% of nitrogen and 0.19% of phosphorous. 3. Continuous reactor has achieved 42% COD removal rate, 62% SS, 10% T-N, and 46% T-P, Effluent COD concentration was 5.8㎎/ℓ, SS 8.13㎎/ℓ, T-P 0.84㎎/ℓ and T-N 4.09㎎/ℓ.

Preparation and Characterization of A Semi-interpenetrating Network Alkaline Anion Exchange Membrane

Yifu Wang,Heting Wan,Dan Wang,Jilin Wang,Lulu Wang,Ruijiang Feng 한국섬유공학회 2018 Fibers and polymers Vol.19 No.1

A series of semi-interpenetrating network (semi-IPN) anion exchange membranes (QCS/St-G8-2-8, Quaternized chitosan/styrene-[maleic alkylene group diethyl bis (octyl dimethyl chloro/bromide), abbreviated as G8-2-8] were prepared via in-situ polymerization by Styrene (St) and G8-2-8 in QCS casting solution. During the process of in-situ polymerization, linearblock polymers (St-G8-2-8) of Styrene and G8-2-8 was constructed, then was mixed with QCS casting solution, followed crosslinking the QCS by glutaraldehyde (GA). With the increasing content of linear block polymer, water uptake and swelling ratio of the composite membrane decreased; This kind of linear structure makes an order arrangement of quaternaryammonium groups which improves the OH− migration efficiency. At 70 oC, the M-30 composite membrane performs a high OH− conductivity of 8.20×10-2 S·cm-1, the methanol permeability is 3.23×10-6 cm-2·s-1 which is still lower than Nafion 115 of 2.42×10-6 cm-2·s-1, but M-30 shows a higher selectivity of 25.3 than Nafion 115 of 11.6. Furthermore, the membranes exhibited excellent thermal stability (≥150 oC), the tensile strength of the composite membrane is in the range of 14-25 MPa and elongation at break is in the range of 16-37 % at room temperature, as well as superior chemical stability in 1.0 M KOH solution for 250 h.

A small hairpin RNA targeting myeloid cell leukemia-1 enhances apoptosis in host macrophages infected with Mycobacterium tuberculosis

Fei-yu Wang,Yu-qing Zhang,Xin-min Wang,Chan Wang,Xiao-fang Wang,Jiang-dong Wu,Fang Wu,Wan-jiang Zhang,Le Zhang 한국미생물학회 2016 The journal of microbiology Vol.54 No.4

Myeloid cell leukemia-1 (Mcl-1) plays an important role in various cell survival pathways. Some studies indicated that the expression of Mcl-1 was upregulated in host cells during infection with the virulent Mycobacterium tuberculosis strain, H37Rv. The present study was designed to investigate the effect of inhibiting Mcl-1 expression both in vivo and in vitro on apoptosis of host macrophages infected with M. tuberculosis using a small hairpin (sh)RNA. Mcl-1 expression was detected by the real time-polymerase chain reaction, western blotting, and immunohistochemistry. Flow cytometry and transmission electron microscopy were used to measure host macrophage apoptosis. We found elevated Mcl-1 levels in host macrophages infected with M. tuberculosis H37Rv. The expression of Mcl-1 was downregulated efficiently in H37Rv-infected host macrophages using shRNA. Knockdown of Mcl-1 enhanced the extent of apoptosis in H37Rv-infected host macrophages significantly. The increased apoptosis correlated with a decrease in M. tuberculosis colony forming units recovered from H37Rv-infected cells that were treated with Mcl-1-shRNA. Reducing Mcl-1 accumulation by shRNA also reduced accumulation of the anti-apoptotic gene, Bcl-2, and increased expression of the pro-apoptotic gene, Bax, in H37Rv-infected host macrophages. Our results showed that specific knockdown of Mcl-1 expression increased apoptosis of host macrophages significantly and decreased the intracellular survival of a virulent strain of M. tuberculosis. These data indicate that interference with Mcl-1 expression may provide a new avenue for tuberculosis therapy.

Green-revertible Chlorina 1 (grc1) is Required for the Biosynthesis of Chlorophyll and the Early Development of Chloroplasts in Rice

Jieqin Li,Yihua Wang,Juntao Chai,Lihua Wang,Chunming Wang,Wuhua Long,Di Wang,Yunlong Wang,Ming Zheng,Cheng Peng,Mei Niu,Jianmin Wan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.5

The nuclear genes involved in chloroplast developmentand chlorophyll biosynthesis must be investigated tounderstand their functions in plant growth and development. In this study, we isolated and identified a unique leaf-colormutant of rice with a green-yellow phenotype before thefour-leaf stage and named the mutation green-revertiblechlorina 1 (grc1). The mutants had significantly lower plantheight, number of tillers, and panicle length and headedsignificantly earlier than the wild type. The levels ofchlorophylls, carotenoids, and chlorophyll precursors werealso lower. The mutation in grc1 affected chloroplastultrastructure, particularly thylakoid development. Geneticanalysis indicated that the green-yellow phenotype wascontrolled by a single recessive gene. We mapped the grc1gene to a 32.4-kb region on the long arm of chromosome 6. Through map-based cloning, we identified a 45-bp insertionin the genomic region of LOC_Os06g40080, which encodeda heme oxygenase. Expression of LOC_Os06g40080 wassignificantly down-regulated in the grc1 mutant. Subcellularlocalization showed that this heme oxygenase was localizedin the chloroplast. In summary, we isolated and identified thegene for grc1, which plays an important role in chlorophyllbiosynthesis and chloroplast development in rice.

A putative plastidial adenine nucleotide transporter, BRITTLE1-3, plays an essential role in regulating chloroplast development in rice (Oryza sativa L.)

Jia Lyu,Yihua Wang,Linglong Liu,Chunming Wang,Yulong Ren,Cheng Peng,Feng Liu,Yunlong Wang,Mei Niu,Di Wang,Ming Zheng,Kunneng Zhou,Shaolu Zhao,Fuqing Wu,Haiyang Wang,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5

Differentiation from proplastids into chloroplasts isa light- and energy-dependent process. How this process isregulated is still poorly understood at the molecular level. We herein report a new putative plastidial adenine nucleotidetransporter, BRITTLE1-3 (referred to as OsBT1-3), encoded bythe rice (Oryza sativa) White Stripe Leaf 2 (WSL2) gene. Loss of OsBT1-3 function results in defective chloroplastbiogenesis, severely reduced photosynthetic efficiency, andfinally a white stripe leaf phenotype in the first four leaves. The expression levels of genes related to chlorophyllbiosynthesis and photosynthesis are drastically reduced,accompanied with over accumulation of reactive oxygenspecies (ROS) in the wsl2 mutant. OsBT1-3 is targeted tothe chloroplasts and it expresses in almost all tissues inplants, especially in young leaves. OsBT1-3 consists of 419amino acids and exhibits features of all mitochondrialcarrier proteins, including a typical transmembrane-spanningdomain and a highly conserved sequence motif designatedas the ‘mitochondrial energy transfer signatures’. Phylogeneticanalysis shows that OsBT1-3 is a putative plastidialadenine nucleotide transporter and is most closely relatedto ZmBT1-2. Together, these observations suggest that thenew putative adenine nucleotide transporter, OsBT1-3,plays an essential role in regulating chloroplast biogenesisand maintenance of ROS homeostasis during rice seedlingde-etiolation.

Chinas Importation of Korean Variety Shows

Wan Wang,Surng-Gahb Jahng 중앙대학교 영상콘텐츠융합연구소 2016 TechArt :Journal of Arts and Imaging Science Vol.3 No.1

INF-SUP METHOD FOR ESTABLISHING INEQUALITIES

WAN-LAN WANG,QIUYAN ZHANG,JIN-YAN MIAO 장전수학회 2022 Advanced Studies in Contemporary Mathematics Vol.32 No.4

This paper systematically summarizes the inf-sup method for establishing inequalities. With different results and specific techniques, we not only use this method to establish the geometric-arithmetic mean inequality, but also establish the new r-th mean inverses.

Effects of wilting and additives on the ensiling quality and in vitro rumen fermentation characteristics of sudangrass silage

Wan, Jiang Chun,Xie, Kai Yun,Wang, Yu Xiang,Liu, Li,Yu, Zhu,Wang, Bing Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.1

Objective: This study was conducted to investigate the effects of molasses and Lactobacillus plantarum on the ensiling quality and in vitro rumen fermentation of sudangrass silage prepared with or without wilting. Methods: The ensiling experiment, measured with 3 replicates, was carried out according to a 2×4 (wilted stages×additives) factorial treatment structure. Dry matter of the fresh (210 g/kg fresh matter) or wilted (305 g/kg fresh matter) sudangrass were ensiled (packed into 5.0-L plastic jars) without additive (control) or with molasses (M), Lactobacillus plantarum (LP), or molasses + Lactobacillus plantarum (M+LP). After 60 days of ensiling, the silages were analyzed for the chemical, fermentation, and in vitro characteristics. Results: After 60 days of ensiling, the fermentation parameters were affected by wilted, the additives and the interactions of wilted with the additives (p<0.05). The M+LP treatment at wilted had higher lactic acid levels and V-score (p<0.05) but lower pH values and butyric acid concentrations than the other treatments. In comparison with sudangrass before ensiling, after ensiling had lower dry matter and higher non-fibrous carbohydrate. The in vitro gas production, in vitro dry matter digestibility, in vitro crude protein digestibility, and in vitro acid fiber detergent digestibility changed under the effects of the additives. Significant interactions were observed between wilted and the additives in terms of in vitro gas production at 48 h, asymptotic gas production, gas production rate, half time, and the average gas production rate. The total volatile fatty acid levels in the additive treatments were higher than those in the control. Conclusion: Wilting and supplementation with molasses and Lactobacillus plantarum had the ability to improve the ensiling quality and in vitro nutrient digestibility of sudangrass silage. The M+LP treatment at wilted exhibited the strongest positive effects on silage quality and in vitro ruminal fermentation characteristics.