Characterization of the Interactions Between Cucumber mosaic virus and Potato virus Y in Mixed Infections in Tomato.

Mascia, Tiziana,Cillo, Fabrizio,Fanelli, Vilma,Finetti-Sialer, Mariella Matilde,De Stradis, Angelo,Palukaitis, Peter,Gallitelli, Donato APS Press 2010 Molecular plant-microbe interactions Vol.23 No.11

<P>Mixed infection with the SON41 strain of Potato virus Y (PVY-SON41) in tomato increased accumulation of RNAs of strains Fny and LS of Cucumber mosaic virus (CMV-Fny and CMV-LS, respectively) and enhanced disease symptoms. By contrast, replication of PVY-SON41 was downregulated by CMV-Fny and this was due to the CMV-Fny 2b protein. The CMV-FnyΔ2b mutant was unable to systemically invade the tomato plant because its movement was blocked at the bundle sheath of the phloem. The function needed for invading the phloem was complemented by PVY-SON41 in plants grown at 22°C whereas this complementation was not necessary in plants grown at 15°C. Mutations in the 2b protein coding sequence of CMV-Fny as well as inhibition of translation of the 2a/2b overlapping region of the 2a protein lessened both the accumulation of viral RNAs and the severity of symptoms. Both of these functions were complemented by PVY-SON41. Infection of CMV-Fny supporting replication of the Tfn-satellite RNA reduced the accumulation of CMV RNA and suppressed symptom expression also in plants mixed-infected with PVY-SON41. The interaction between CMV and PVY-SON41 in tomato exhibited different features from that documented in other hosts. The results of this work are relevant from an ecological and epidemiological perspective due to the frequency of natural mixed infection of CMV and PVY in tomato.</P>

Unprecedented Tris-Phosphido-Bridged Triangular Clusters with 42 Valence Electrons. Chemical, Electrochemical and Computational Studies of their Formation and Stability

Funaioli, Tiziana,Leoni, Piero,Marchetti, Lorella,Albinati, Alberto,Rizzato, Silvia,Fabrizi de Biani, Fabrizia,Ienco, Andrea,Manca, Gabriele,Mealli, Carlo American Chemical Society 2013 Inorganic Chemistry Vol.52 No.8

<P>This paper presents the synthesis and structural characterization of the unprecedented tris-phosphido-bridged compounds Pt<SUB>3</SUB>(μ-PBu<SUP><I>t</I></SUP><SUB>2</SUB>)<SUB>3</SUB>X<SUB>3</SUB> (X = Cl, Br, I), having only 42 valence electrons, while up to now analogous clusters typically have 44e<SUP>–</SUP>. The new species were obtained by an apparent bielectronic oxidation of the 44e<SUP>–</SUP> monohalides Pt<SUB>3</SUB>(μ-PBu<SUP><I>t</I></SUP><SUB>2</SUB>)<SUB>3</SUB>(CO)<SUB>2</SUB>X with the corresponding dihalogen X<SUB>2</SUB>. Their X-ray structures are close to the <I>D</I><SUB>3<I>h</I></SUB> symmetry, similarly to the 44e<SUP>–</SUP> analogues with three terminal carbonyl ligands. The products were also obtained by electrochemical oxidation of the same monohalides in the presence of the corresponding halide. In a detailed study on the formation of Pt<SUB>3</SUB>(μ-PBu<SUP><I>t</I></SUP><SUB>2</SUB>)<SUB>3</SUB>I<SUB>3</SUB>, the redox potentials indicated that I<SUB>2</SUB> can only perform the first monoelectronic oxidation but is unsuited for the second one. Accordingly, the 43e<SUP>–</SUP> intermediate [Pt<SUB>3</SUB>(μ-PBu<SUP><I>t</I></SUP><SUB>2</SUB>)<SUB>3</SUB>(CO)<SUB>2</SUB>I]<SUP>+</SUP> was ascertained to play a key role. Another piece of information is that, together with the fully oxidized product Pt<SUB>3</SUB>(μ-PBu<SUP><I>t</I></SUP><SUB>2</SUB>)<SUB>3</SUB>I<SUB>3</SUB>, the transient 44e<SUP>–</SUP> species [Pt<SUB>3</SUB>(μ-PBu<SUP><I>t</I></SUP><SUB>2</SUB>)<SUB>3</SUB>(CO)<SUB>3</SUB>]<SUP>+</SUP> is formed in the early steps of the reaction. In order to extract detailed information on the formation pathway, involving both terminal ligand substitutions and electron transfer processes, a DFT investigation has been performed and all the possible intermediates have been defined together with their associated energy costs. The profile highlights many important aspects, such as the formation of an appropriate couple of 43e<SUP>–</SUP> intermediates having different sets of terminal coligands, and suitable redox potentials for the transfer of one electron. Optimizations of 45e<SUP>–</SUP> associative intermediates in the ligand substitution reactions indicate their possible involvement in the redox process with reduction of the overall energy cost. Finally, according to MO arguments, the unique stability of the 42e<SUP>–</SUP> phosphido-bridged Pt<SUB>3</SUB> clusters can be attributed to the simultaneous presence of three terminal halides.</P><P>This paper presents the synthesis and structural characterization of the unprecedented tris-phosphido-bridged compounds Pt<SUB>3</SUB>(μ-PBu<SUP><I>t</I></SUP><SUB>2</SUB>)<SUB>3</SUB>X<SUB>3</SUB> (X = Cl, Br, I), having only 42 valence electrons, while up to now analogous clusters typically have 44e<SUP>−</SUP>. The new species were obtained by an apparent bielectronic oxidation of the 44e<SUP>−</SUP> monohalides Pt<SUB>3</SUB>(μ-PBu<SUP><I>t</I></SUP><SUB>2</SUB>)<SUB>3</SUB>(CO)<SUB>2</SUB>X with the corresponding dihalogen X<SUB>2</SUB>. According to MO arguments, the unique stability of the 42e<SUP>−</SUP> phosphido-bridged Pt<SUB>3</SUB> clusters can be attributed to the simultaneous presence of three terminal halides.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/inocaj/2013/inocaj.2013.52.issue-8/ic400251y/production/images/medium/ic-2013-00251y_0014.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ic400251y'>ACS Electronic Supporting Info</A></P>

Programmed-release intraosseus anesthesia as an alternative to lower alveolar nerve block in lower third molar extraction: a randomized clinical trial

Renato Pol,Tiziana Ruggiero,Marta Bezzi,Davide Camisassa,Stefano Carossa 대한치과마취과학회 2022 Journal of Dental Anesthesia and Pain Medicine Vol.22 No.3

Background: Intraosseous anesthesia is the process by which an anesthetic solution, after penetration of the cortical bone, is directly injected into the spongiosa of the alveolar bone supporting the tooth. This study aimed to compare the effectiveness of the traditional inferior alveolar nerve block (IANB) and computerized intraosseous anesthesia in the surgical extraction of impacted lower third molars, compare their side effects systemically by monitoring heart rate, and assess patients’ a posteriori preference of one technique over the other. Methods: Thirty-nine patients with bilaterally impacted third molars participated in this study. Each patient in the sample was both a case and control, where the conventional technique was randomly assigned to one side (group 1) and the alternative method to the contralateral side (group 2). Results: The traditional technique was faster in execution than anesthesia delivered via electronic syringe, which took 3 min to be administered. However, it was necessary to wait for an average of 6 ± 4 min from the execution to achieve the onset of IANB, while the latency of intraosseous anesthesia was zero. Vincent's sign and lingual nerve anesthesia occurred in 100% of cases in group 1. In group 2, Vincent’s sign was recorded in 13% of cases and lingual anesthesia in four cases. The average duration of the perceived anesthetic effect was 192 ± 68 min in group 1 and 127 ± 75 min in group 2 (P < 0.001). The difference between the heart rate of group 1 and group 2 was statistically significant. During infiltration in group 1, heartbeat frequency increased by 5 ± 13 beats per minute, while in group 2, it increased by 22 ± 10 beats per minute (P < 0.001). No postoperative complications were reported for either technique. Patients showed a preference of 67% for the alternative technique and 20% for the traditional, and 13% of patients were indifferent. Conclusion: The results identified intraosseous anesthesia as a valid alternative to conventional anesthesia in impacted lower third molar extraction.

Functional characterization of a minimal sequence essential for the expression of human TLX2 gene

( Silvia Borghini ),( Tiziana Bachetti ),( Monica Fava ),( Marco Di Duca ),( Roberto Ravazzolo ),( Isabella Ceccherini ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.12

A Xenogenic Bone Derivative as a Potential Adjuvant for Bone Regeneration and Implant Osseointegration: An In Vitro Study

Graziella Bellone,Barbara Vizio,Tiziana Scirelli,Giorgio Emanuelli 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.3

Several clinical conditions may limit the success of bone regeneration and/or implant osseointegration. For this reason, many compounds have been tested for their ability to stimulate this biological process. Synthetic hydroxyapatite (HA), mimicking natural bone hydroxyapatite, and extra-cellular matrix proteins, such as type I collagen, are potential candidates. However, the synthetic origin of HA and the denaturing conditions required for extracting collagen from skin and derma are sources of potential drawbacks. This study examines the in vitro effects of a natural bone derivative (NBD) extracted from equine bone and containing both natural, non-synthetic bone hydroxyapatite and native, non-denatured, type I bone collagen as a possible active compound for stimulating bone regeneration and implant osseointegration. The activity of NBD was tested on bone marrow stromal cells (BMSCs), evaluating their growth/viability by the methylthiazol tetrazolium (MTT) assay and their migration potential by a scratch assay. Moreover, expression of the hyaluronic acid receptor (CD44) and the C-X-C chemokine receptor type 4 (CXCR4, CD184) on the surface of BMSCs was assessed by flow cytometry, and the release of Transforming Growth Factor (TGF)-b, Interleukin (IL)-1a and IL-6 was quantified using an enzyme-linked immunosorbent assay (ELISA). The effect of NBD-coated implants on human osteoblasts was tested by measuring alkaline phosphatase (ALP) activity with the p-nitrophenyl phosphate (pNPP) degradation test. NBD stimulated BMSC growth/viability, migration, CD184 surface expression and the release of TGF-b1. NBD-coated implants increased ALP activity of human osteoblasts. These results indicate that NBD may be an adjuvant to accelerate both bone regeneration and osseointegration.

Application of a novel approach to modelling the supercritical extraction kinetics of oil from two sets of chia seeds

David Villanueva-Bermejo,Tiziana Fornari,Maria V. Calvo,Javier Fontecha,Jose A.P. Coelho,Rui M. Filipe,Roumiana P. Stateva 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.82 No.-

The kinetics of the supercriticalfluid extraction of edible and discarded chia seeds was studied andmodelled for thefirst time. The total oil was removed at 45 MPa and 60 C after 240 min. The extractionkinetics was simulated using a dynamic model in gPROMS ModelBuilder environment and the kineticparameters estimated. Triolein was chosen as a model compound of the chia oil. The agreement betweenthe experimental yields and those calculated by the model was good with deviations in the range(1.2–6.6) %, except at 25 MPa and 60 C (AARD = 9.5%).

Talazoparib versus Chemotherapy in Patients with HER2-Negative Advanced Breast Cancer and a Germline BRCA1/2 Mutation Enrolled in Asian Countries: Exploratory Subgroup Analysis of the Phase III EMBRACA Trial

이경훈,손주혁,Annabel Goodwin,Tiziana Usari,Silvana Lanzalone,임석아,김성배 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose We evaluated study outcomes in patients enrolled in Asian regions in the phase III EMBRACA trial of talazoparib vs. chemotherapy. Materials and Methods Patients with human epidermal growth factor receptor 2–negative germline BRCA1/2-mutated advanced breast cancer who received prior chemotherapy were randomized 2:1 to talazoparib 1 mg/day or chemotherapy (physician’s choice). Primary endpoint was progression-free survival (PFS) per independent central review in the intent-to-treat (ITT) population. This post-hoc analysis evaluated efficacy/safety endpoints in the ITT population of patients enrolled in Asian regions. Results Thirty-three patients were enrolled at Asian sites (talazoparib, n=23; chemotherapy, n=10). Baseline characteristics were generally comparable with the overall EMBRACA population. In Asian patients, median PFS was 9.0 months (95% confidence interval [CI], 3.0 to 15.2) for talazoparib and 7.1 months (95% CI, 1.2 to not reached) for chemotherapy (hazard ratio [HR], 0.74 [95% CI, 0.22 to 2.44]). Objective response rate was numerically higher for talazoparib vs. chemotherapy (62.5% [95% CI, 35.4 to 84.8] vs. 25.0% [95% CI, 3.2 to 65.1]). Median overall survival was 20.7 months (95% CI, 9.4 to 40.1) versus 21.2 months (95% CI, 2.7 to 35.0) (HR, 1.41 [95% CI, 0.49 to 4.05]). In Asian patients, fewer grade 3/4 adverse events (AEs), serious AEs (SAEs), grade 3/4 SAEs, and AEs resulting in dose reduction/discontinuation occurred with talazoparib than chemotherapy; for talazoparib, the frequency of these events was lower in Asian patients versus overall EMBRACA population. Conclusion In this subgroup analysis, talazoparib numerically improved efficacy versus chemotherapy and was generally well tolerated in Asian patients, with fewer grade 3/4 treatment-emergent AE (TEAEs), SAEs, and TEAEs leading to dose modification vs. the overall EMBRACA population.

Neural stem cells from a mouse model of Rett syndrome are prone to senescence, show reduced capacity to cope with genotoxic stress, and are impaired in the differentiation process

Nicola Alessio,Francesco Riccitiello,Tiziana Squillaro,Stefania Capasso,Stefania Del Gaudio,Giovanni Di Bernardo,Marilena Cipollaro,Mariarosa A. B. Melone,Gianfranco Peluso,Umberto Galderisi 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Several aspects of stem cell life are governed by epigenetic variations, such as DNA methylation, histone modifications, and chromatin remodeling. Epigenetic events are also connected with the impairment of stem cell functions. For example, during senescence, there are significant changes in chromatin organization that alter transcription. The MECP2 protein can bind methylated cytosines and contribute to regulating gene expression at one of the highest hierarchical levels. Researchers are particularly interested in this protein, as up to 90% of Rett syndrome patients have an MECP2 gene mutation. Nevertheless, the role of MECP2 in this disease remains poorly understood. We used a mouse model of Rett syndrome to evaluate whether residual MECP2 activity in neural stem cells (NSCs) induced the senescence phenomena that could affect stem cell function. Our study clearly demonstrated that the reduced expression of MECP2 is connected with an increase in senescence, an impairment in proliferation capacity, and an accumulation of unrepaired DNA foci. Mecp2+/− NSCs did not cope with genotoxic stress in the same way as the control cells did. Indeed, after treatment with different DNA-damaging agents, the NSCs from mice with mutated Mecp2 accumulated more DNA damage foci (γ-H2AX+) and were more prone to cell death than the controls. Senescence in Mecp2+/− NSCs decreased the number of stem cells and progenitors and gave rise to a high percentage of cells that expressed neither stem/progenitor nor differentiation markers. These cells could be senescent and dysfunctional.

HEATED INTRACLUSTER GAS AND RADIO CONNECTIONS: THE SINGULAR CASE OF MKW 3S

MAZZOTTA PASQUALE,BRUNETTI GIANFRANCO,GIACINTUCCI SIMONA,VENTURI TIZIANA,BARDELLI SANDRO The Korean Astronomical Society 2004 Journal of The Korean Astronomical Society Vol.37 No.5

Similarly to other cluster of galaxies previously classified as cooling flow systems, the Chandra observation of MKW 3s reveals that this object has a complex X-ray structure hosting both a X-ray cavity and a X-ray filament. Unlike the other clusters, however, the temperature map of the core of MKW 3s shows the presence of extended regions of gas heated above the radially averaged gas temperature at any radius. As the cluster does not show evidences for ongoing major mergers Mazzotta et al. suggest a connection between the heated gas and the activity of the central AGN. Nevertheless, due to the lack of high quality radio maps, this interpretation was controversial. In this paper we present the results of two new radio observations of MKW 3s at 1.28 GHz and 604 MHz obtained at the GMRT. Together with the Chandra observation and a separate VLA observation at 327 MHz from Young, we show unequivocal evidences for a close connection between the heated gas region and the AGN activity and we briefly summarize possible implications.

Hybrid membranes for the production of blood contacting surfaces: physicochemical, structural and biomechanical characterization

Martina Todesco,Carlo Zardin,Laura Iop,Tiziana Palmosi,Pietro Capaldo,Filippo Romanato,Gino Gerosa,Andrea Bagno 한국생체재료학회 2021 생체재료학회지 Vol.25 No.3

Background: Due to the shortage of organs’ donors that limits biological heart transplantations, mechanical circulatory supports can be implanted in case of refractory end-stage heart failure to replace partially (Ventricular Assist Device, VAD) or completely (Total Artificial Heart, TAH) the cardiac function. The hemocompatibility of mechanical circulatory supports is a fundamental issue that has not yet been fully matched; it mostly depends on the nature of blood-contacting surfaces. Methods: In order to obtain hemocompatible materials, a pool of hybrid membranes was fabricated by coupling a synthetic polymer (polycarbonate urethane, commercially available in two formulations) with a decellularized biological tissue (porcine pericardium). To test their potential suitability as candidate materials for realizing the blood-contacting surfaces of a novel artificial heart, hybrid membranes have been preliminarily characterized in terms of physicochemical, structural and mechanical properties. Results: Our results ascertained that the hybrid membranes are properly stratified, thus allowing to expose their biological side to blood and their polymeric surface to the actuation system of the intended device. From the biomechanical point of view, the hybrid membranes can withstand deformations up to more than 70 % and stresses up to around 8 MPa. Conclusions: The hybrid membranes are suitable for the construction of the ventricular chambers of innovative mechanical circulatory support devices.