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Li, Xiaohua,Thwe, Aye Aye,Park, Chang Ha,Kim, Sun Ju,Arasu, Mariadhas Valan,Abdullah Al-Dhabi, Naif,Lee, Sook Young,Park, Sang Un Informa UK (TaylorFrancis) 2017 Biotechnology, biotechnological equipment Vol.31 No.2
<P>The main flavonoid compound rutin is highly expressed in the crop plant buckwheat (Fagopyrum tataricum (L.) Gaertn.) and plays important roles in the human diet. In this study, phenylpropanoid production in buckwheat hairy roots was evaluated following ethephon treatment. Using quantitative real-time polymerase chain reaction and high-performance liquid chromatography, we analysed the relationship between flavonoid and anthocyanin biosynthetic pathway gene regulation and the relative accumulation of the secondary compounds in ethephon-treated buckwheat hairy roots. Generally, the transcription of the biosynthetic pathway genes varied between the treated samples and controls. Most of the flavonoid biosynthetic genes were upregulated by ethephon, typically after four days of treatment. The application of 0.5 mg/L ethephon markedly induced anthocyanin production in hairy roots compared to that induced by the other concentrations tested (0, 1 and 2 mg/L). These data indicate that anthocyanin biosynthesis may play an important role in the response of buckwheat to ethephon-induced stress.</P>
Kim, Yeon Bok,Thwe, Aye Aye,Kim, Ye Ji,Li, Xiaohua,Kim, Haeng Hoon,Park, Phun Bum,Suzuki, Tastsuro,Kim, Sun-Ju,Park, Sang Un American Chemical Society 2013 Journal of agricultural and food chemistry Vol.61 No.17
<P>Tartary buckwheat (Fagopyrum tataricum Gaertn.) contains a high level of flavonoid compounds, which have beneficial and pharmacological effects on health. In this study, we isolated full-length cDNAs encoding hydroxycinnamoyl-coenzyme A quinate hydroxycinnamoyltransferase (HQT) and <I>p</I>-coumarate 3′-hydroxylase (C3H), which are involved in chlorogenic acid (CGA) biosynthesis. We examined the expression levels of HQT and C3H using real-time RT-PCR in different organs and sprouts of two tartary buckwheat cultivars (Hokkai T8 and T10) and analyzed CGA content using high-performance liquid chromatography. Among the organs, the flowers in both cultivars showed the highest levels of CGA. We concluded that the expression pattern of <I>FtHQT</I> and <I>FtC3H</I> did not match the accumulation pattern of CGA in different organs of T8 and T10 cultivars. Gene expression and CGA content varied between the cultivars. We presume that <I>FtHQT</I> and <I>FtC3H</I> levels might be controlled by multiple metabolic pathways in different organs of tartary buckwheat. Probably, <I>FtC3H</I> might have a greater effect on CGA biosynthesis than <I>FtHQT</I>. Our results will be helpful for a greater understanding of CGA biosynthesis in tartary buckwheat.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2013/jafcau.2013.61.issue-17/jf4000659/production/images/medium/jf-2013-000659_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf4000659'>ACS Electronic Supporting Info</A></P>