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Li, Xiaohua,Thwe, Aye Aye,Park, Chang Ha,Kim, Sun Ju,Arasu, Mariadhas Valan,Abdullah Al-Dhabi, Naif,Lee, Sook Young,Park, Sang Un Informa UK (TaylorFrancis) 2017 Biotechnology, biotechnological equipment Vol.31 No.2
<P>The main flavonoid compound rutin is highly expressed in the crop plant buckwheat (Fagopyrum tataricum (L.) Gaertn.) and plays important roles in the human diet. In this study, phenylpropanoid production in buckwheat hairy roots was evaluated following ethephon treatment. Using quantitative real-time polymerase chain reaction and high-performance liquid chromatography, we analysed the relationship between flavonoid and anthocyanin biosynthetic pathway gene regulation and the relative accumulation of the secondary compounds in ethephon-treated buckwheat hairy roots. Generally, the transcription of the biosynthetic pathway genes varied between the treated samples and controls. Most of the flavonoid biosynthetic genes were upregulated by ethephon, typically after four days of treatment. The application of 0.5 mg/L ethephon markedly induced anthocyanin production in hairy roots compared to that induced by the other concentrations tested (0, 1 and 2 mg/L). These data indicate that anthocyanin biosynthesis may play an important role in the response of buckwheat to ethephon-induced stress.</P>
Kim, Yeon Bok,Thwe, Aye Aye,Li, Xiaohua,Tuan, Pham Anh,Zhao, Shicheng,Park, Chun Geon,Lee, Jong Won,Park, Sang Un Humana Press 2014 Applied biochemistry and biotechnology Vol.173 No.8
<P>Astragalus membranaceus is one of the important medicinal plant in China and Korea. It is used to increase metabolism and digestion, enhance the immune system, and promote the healing of wounds and injuries. In the present study, we used quantitative real-time PCR to investigate the expression of genes related to the biosynthesis of flavonoids, in addition to high-performance liquid chromatography to assess calycosin and calycosin-7-O-β-D-glucoside accumulation, in the different plant organs of A. membranaceus. The transcript levels of all genes (AmPAL, AmC4H, Am4CL, AmCHS, AmCHR, AmCHI, AmIFS, AmI3'H, and AmUCGT) involved in calycosin and calycosin-7-O-β-D-glucoside biosynthesis were the highest in the flower. Calycosin content was ordered as follows: leaf (145.56 μg/g dry weight [DW]) > stem (18.3 μg/g DW) > root (1.64 μg/g DW) > flower (0.09 μg/g DW), whereas calycosin-7-O-β-D-glucoside content was ordered as follows: root (4.88 μg/g DW) > stem (3.86 μg/g DW) > leaf (2.0 μg/g DW) > flower (not detected). All genes exhibited the highest transcription levels in the flower, whereas calycosin and its glycoside content were the highest in the leaf and root, respectively. Our results indicate that the enhancement of calycosin-7-O-β-D-glucoside in the roots may originate from high calycosin accumulation in the stem and leaf. Thus, the mechanisms regulating calycosin and calycosin-7-O-β-D-glucoside content differ in the different organs of A. membranaceus. The results are expected to provide baseline information from which the mechanism of flavonoid biosynthesis in the different organs of A. membranaceus may be elucidated.</P>