企業內 人力開發에 關한 硏究

林義澤 울산대학교 1990 연구논문집 Vol.21 No.2

企業의 長期的인 立場에서 繼續 成長 發展하기 爲하여 人材確保를 積極的으로 推進해야 하며, 有能한 人材確保를 爲하여 敎育訓練에 投資를 아끼지 않아야 한다. 앞으로의 企業의 敎育訓練은 人間重視의 敎育을 展開함으로서 組織에서 차지하는 人間의 위대성을 認識시키고 健全한 職業觀을 涵養하여 勞使가 모두 主人이라는 事實을 體得하게 하는데 이바지해야 할 것이다. From the far-sighted point of view, it is argued that the industry must make its positive efforts in order to ensure able man-power for its continuous growth and progress and that the industry must make a sufficient investment to the job training of its members. It is also argued that the future job training of the industry must make emphasis on the human aspect and furthermore make its members recognize the greatness of human being and also help them cultivate sound job-view, which makes it possible to understand that the employer and the employees is one some body not the opposite one.

마르크스ㆍ레닌主義 극복에 關한 硏究

임의택 울산대학교 1990 연구논문집 Vol.21 No.2

社會의 進化는 必然的 革命이 아닌 目的活動的 改善과 漸次的 進化에 依한다. 生産樣式은 生産의 區分에 不過하고 生産力과 生産關係는 經濟的 槪念이 아니다. 生産力의 發展은 技術과 科學發展에 直結되고 이는 다시 人間精神 作用의 所産이며 單純한 物質의 힘이 아니다. 昨今의 東歐事態는 지난 40年 以上 적어도 外形上으로 굳건히 維持되어온 마르크스 ·레닌主義가 종말로 치닫고 있음을 反證하는 것이다. The social improvement depends on the gradual evolution of the purpose-sensitive a ctivities, not on the inevitable revolution. The mode of production is just a means of production analysis and the relationship of production and productivity is not a economic notion. The advancement of productivity directly relates to the progress of science and technology and also it is the result of the human mental activities, not a simple material energy. The recent affairs of Eastern Europe is strong evidence to foretell the end of Marks-Leninism which has been un fallingly, preserved over the last forty years at least superficially.

Protective Effect of Bcl-2 in NS0 Myeloma Cell Culture is Greater in More Stressful Environments

Tey, B.T.,Al-Rubeai, M. The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.6

In the present study, the protective effects of Bcl-2 over-expression in a suspension culture (without any adaptation) and spent medium (low nutrient and high toxic metabolite conditions) were investigated. In the suspension culture without prior adaptation, the viability of the control cell line fall to 0% by day 7, whereas the Bcl-2 cell line had a viability of 65%. The difference in the viability and viable cell density between the Bcl-2 and control cell lines was more apparent in the suspension culture than the static culture, and became even more apparent on day 6. Fluorescence microscopic counting revealed that the major mechanism of cell death in the control cell line in both the static and suspension cultures was apoptosis. For the Bcl-2 cell lines, necrosis was the major mode of cell death in the static culture, but apoptosis became equally important in the suspension culture. When the NS0 6A1 cell line was cultured in spent medium taken from a 14 day batch culture, the control cell line almost completely lost its viability by day 5, whereas, the Bcl-2 still had a viability of 73%. The viable cell density and viability of the Bcl-2 cell line cultivated in fresh medium were 2.2 and 2.7 fold higher, respectively, than those of the control cultures. However, the viable cell density and viability of the Bcl-2 cultivated in the spent medium were 8.7 and 7.8 fold higher, respectively, than those of the control cultures. Most of the dead cells in the control cell line were apoptotic; whereas, the major cell death mechanisms in the Bcl-2 cell line were necrotic.

BcI-2 Over-expression Reduced the Serum Dependency and Improved the Nutrient Metabolism in a NS0 Cells Culture

Tey Beng Ti,Al-Rubeai Mohamed The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

The over-expression of Bcl-2 has greatly improved the culture period, specific growth rate, and maximum viable cell density of NS0 cells culture under low serum condition. Further analysis of these data suggests that a saturation model of the Monod type can be used to represent the relationships of specific growth rate and initial serum concentration. The ${\mu}_{max}$ and $K_s$ for the Bcl-2 cell line is $0.927day^{-1}\;and\;0.947\%(v/v)$ respectively, which are $21\%$ greate and $7\%$ lower respectively than its control counterpart. Study on the amino acid supplementation revealed that Bcl-2 cell lines possess greater improvement in the specific growth rate and maximum viable cell density compared to the control cell lines. A further increase in the amino acid supplementation has resulted a $17\%$ decrease in specific growth rate and no improvement in maximum viable cell density in the control culture. However, the Bcl-2 cell line exhibited a better growth characteristic in this culture condition compared to that of control cell lines. The higher specific growth rate and maximum viable cell density of the Bcl-2 cell line in medium fortified with serum and MEM EM suggested a more efficient nutrient metabolism compared to that in the control cell line. The low serum and amino acid utilisation rate and the higher cell yield may prove to be important in the development of serum/protein free culture.

A Novel Remote Rehabilitation System with the Fusion of Noninvasive Wearable Device and Motion Sensing for Pulmonary Patients

Tey, Chuang-Kit,An, Jinyoung,Chung, Wan-Young Hindawi 2017 Computational and mathematical methods in medicine Vol.2017 No.-

<P>Chronic obstructive pulmonary disease is a type of lung disease caused by chronically poor airflow that makes breathing difficult. As a chronic illness, it typically worsens over time. Therefore, pulmonary rehabilitation exercises and patient management for extensive periods of time are required. This paper presents a remote rehabilitation system for a multimodal sensors-based application for patients who have chronic breathing difficulties. The process involves the fusion of sensory data—captured motion data by stereo-camera and photoplethysmogram signal by a wearable PPG sensor—that are the input variables of a detection and evaluation framework. In addition, we incorporated a set of rehabilitation exercises specific for pulmonary patients into the system by fusing sensory data. Simultaneously, the system also features medical functions that accommodate the needs of medical professionals and those which ease the use of the application for patients, including exercises for tracking progress, patient performance, exercise assignments, and exercise guidance. Finally, the results indicate the accurate determination of pulmonary exercises from the fusion of sensory data. This remote rehabilitation system provides a comfortable and cost-effective option in the healthcare rehabilitation system.</P>

Optimal Conditions for Hepatitis B Cove Antigen Production in Shaked Flask Fermentation

Tey Beng Ti,Yong Kok Hoe,Ong Hong Puay,Ling Tau Chuan,Ong Swee Tin,Tan Yan Peng,Ariff Avbakariya,Tan Wen Siang The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.5

The effects of various environmental factors such as pH (5, 6, 7, 8 and 9), temperature (30, 37 and $40^{\circ}C$) and rotational speed (150, 200 and 250 rpm) on the growth and the hepatitis B core antigen (HBcAg) production of Escherichia coli W3110IQ were examined in the present Study. The highest growth rate is achieved at pH 7, $37^{\circ}C$ and at a rotational Speed of 250 rpm which is 0.927 $h^{-1}$. The effect of pH on cell growth is more substantial compared to other parameters; it recorded a $123\%$ different between the highest growth rate (0.927 $h^{-1}$) at pH 7 and lowest growth at pH 5. The highest protein yield is achieved at pH 9, rotational speed of 250 rpm and $40^{\circ}C$. The yield of protein at pH 7 is $154\%$ higher compared to the lowest yield achieved at pH 5. There is about $28\%$ different of the protein yield for the E. coli cultivated at 250 rpm compared to that at 150 rpm which has the lowest HBcAg yield. The yield of protein at $40^{\circ}C$ is $38\%$ higher compared to the lowest yield achieved at $30^{\circ}C$.

Production of Hepatitis B Core Antigen in a Stirred Tank Bioreactor: The Influence of Temperature and Agitation

Tey, Beng Ti,Chua, Mung Ing,Chua, Ghee Sung,Ng, Michelle Yeen Tan,Biak, Dayang Radiah Awang,Tan, Wen Siang,Ling, Tau Chuan The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.2

The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli$(0.844 h^{-1})$ was achieved at a temperature of $37^{\circ}C$ and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W3110lQ in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of $44^{\circ}C$ and an agitation speed of 250 rpm. The relative protein concentration at $44^{\circ}C$ is 30 and 6% higher compared to that at 30 and $37^{\circ}C$, respectively.

The Performance of Anion Exchange Expanded Bed Adsorption Chromatography on the Recovery of G6PDH from Unclarified Feedstock with High Biomass Concentration

Beng Ti Tey,Yen Mei Chow,Mohd Nordin Ibrahim,Arbakariya Ariff,Tau Chuan Ling 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.5

The bed stability of Streamline DEAE (ρ = 1.2 g/mL) in a 20 mm (i.d.) glass expanded bed contactor, and its performance on the recovery of glucose 6-phosphate dehydrogenase (G6PDH) from unclarified yeast homogenate were investigated. A residence time distribution study showed that a stable expanded bed was achieved. The theoretical plate and Bodenstein numbers determined were 25 and 53, respectively. A recovery yield of 87% and purification factor of 4.1 were achieved in the operation using 5% (w/v) biomass concentration feedstock. The performance of the anion exchange EBAC was still considerable good at a biomass concentration as high as 15% (w/v).

Production of Hepatitis B Core Antigen in a Stirred Tank Bioreactor: The Influence of Temperature and Agitation

Beng Ti Tey,Mung Ing Chua,Ghee Sung Chua,Michelle Yeen Tan Ng,Dayang Radiah Awang Biak,Wen Siang Tan,Tau Chuan Ling 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.2

The influence of temperature and agitation on the growth of Escherichia coli expressing hepatitis B core antigen (HBcAg) in stirred tank bioreactor were investigated. The highest specific growth rate for E. coli (0.844 h-1) was achieved at a temperature of 37°C and an agitation speed of 250 rpm. The activation energy for the growth of the E. coli strain W3110IQ in the stirred tank bioreactor was estimated to be 11 kcal/mol. The highest protein yield was achieved at a temperature of 44°C and an agitation speed of 250 rpm. The relative protein concentration at 44°C is 30 and 6% higher compared to that at 30 and 37°C, respectively.

Clinical Relevance and Functional Role of Nuclear Met in Hepatocellular Carcinoma

( Sze Keong Tey ),( Edith Yuk Ting Tse ),( Frankie Chi Fat Ko ),( Xiao Wen Mao ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Met is a receptor tyrosine kinase which triggers a wide range of normal physiological signaling cascades. However, a perturbation of the Met pathway is commonly found in human cancers. Emerging evidence has shown the presence of nuclear Met in some cancerous tissues and cell lines, suggesting that nuclear Met could have unexplored functions in the nucleus. The present study aimed to assess the expression and functions of nuclear Met in hepatocellular carcinoma (HCC). Methods: Nuclear Met expression of 103 clinicopathologically characterized HCC paired samples was examined by immunohistochemistry using an antibody against the carboxyl terminus of Met. Statistical analyses were applied to evaluate the association of nMet with different clinical parameters. Nuclear localization of Met was determined by western blot analysis and immunofluorescence microscopy. Met cytoplasmic fragments were characterized by in vitro functional assay such as migration, invasion and proliferation in HCC cells. Nude mice model was employed to investigate the in vivo functional impact of nuclear Met. Results: Nuclear Met is overexpressed in nearly 90% of HCC paired samples and its expression is progressively increased along HCC development from non-tumorous liver tissue to advanced HCC. Nonetheless, nuclear Met overexpression is significantly associated with venous invasion and poorer overall survival. We found that nuclear Met, which has a lower molecular weight than Met, could only be detected using an antibody against the carboxyl terminus of Met (C28) in tumorous tissues. This finding strongly suggests that nuclear Met only comprises of the carboxyl cytoplasmic region of full length Met. Moreover, both western blot analysis of nuclear fraction of HCC cells and immunofluorescence confirmed the nuclear localization of Met. We designed construct J1, J3 and T2 that encode Met fragment truncated after tyrosine residues D972 and P1027 in the juxtamembrane region and after tyrosine kinase domain beginning at L1157, respectively. Immunofluorescence microscopy showed both J1 and J3 constructs are dominantly expressed in the nucleus whereas T2 construct is expressed in the cytoplasm. These observations indicated the region in between J1 and T2 as the important region that facilitates the nuclear localization of Met. In vitro functional assay showed that nMet significantly promoted HCC cell proliferation and anchorage independent growth. It also significantly augmented HCC cell migration and invasiveness. Besides that, nMet also enhanced HCC tumor formation in animal model. Furthermore, we showed that nMet promoted tumor invasiveness and aggressiveness through NF-κ B/MMP2 pathway. Conclusions: Nuclear Met is overexpressed and associated with venous invasion and poorer overall survival in HCC. We found that nuclear Met is actually the carboxyl terminal fragment of Met and translocates into nucleus to promote invasiveness in HCC cells.