그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력

최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1

An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.

중합효소연쇄반응을 이용한 Helicobacter pylori검출

최태열,박경남,강정옥,서일혜 대한감염학회 1997 감염 Vol.29 No.5

배 경 : H.pylori는 위염, 위궤양 재발과 밀접한 관계를 갖고 있기 때문에 검사실적 진단은 정확하여야 한다. H. pylori의 세균배양이 가장 바람직하지만 시설이나 노력면에서 많은 어려움이 있다. 이에 저자는 H. pylori의 세균배양과 더불어 PCR 을 실시하여 PCR의 유용성을 판단코져 다음과 같은 실험을 실시 하였다. 방 법 : 위장 장애를 호소하여 위내시경과 생검을 실시한 247명의 병리조직소견에 따라 정상대조군(57명), 만성위염(131명), 활동성만성위염(19명), 만성궤양(8명), 위암(32명) 으로 분류하였다. 세균배양은 brain heart infusion egg yolk agar선택배지를 사용하였고 PCR은 usease A gene sequence 를 증폭할수 있는 primer 2쌍을 선택하여 nested PCR 을 실시하였다. 결 과 : 세균배양에 의한 H. pylori의 검출율은 100명(40%) 였으며, PCR 에 의한 H.pylori의 검출율은 179명(72%) 으로 PCR 법이 세균배양법보다 검출율이 높았다(P:<0.05, Chi-square test, SPSS, ver7.0, USA). 세균배양과 PCR 모두 음성이 68명, 모두양성이 100명, 세균배양음성 PCR양성이 79명이였으며 PCR의 예민도는 0.1pg DNA(1 bacterial cell)였다. 일반세균을 이용한 특이도 검사에서 양성인 예는 없었다. 결 론 : 상기 결과로 미루어 보아 위생검조직에서 H.pylori의 검출은 연구자들이 사용한 PCR 법이 세균배양법보다 신속 정확하였다. Background : Helicobacter pyloir has been implicated in the pathogenesis of active chronic gastritis and peptic ulcer disease in man. Thus, diagnosis and treatment of H. pylori infection are now of growing importance in ucle management. A variety of noninvasive and invasive methods have been described for the detection of H. pylori, but all of these techniques have disadvantages such as time consuming or insensitivity. So we describe the polymerase chain reaction(PCR) assay for the sensitive and specific detection of H. pylori. Methods : Gastric biopsy specimens were obtained from 147 patients undergoing endoscopic examinations at Hayang University Hospital. One half of the specimen was processed for routine culture and the other half for PCR. Bacterial genomic DNA from gastric biopsies are extracted by Instagene. Two sets of primer pairs derived from the nucleotide sequence of the urease A gene of H. pylori were used. Result: H. pylori was cultured in 100(40%) cases and PCR assay deteted 179(72%) cases (P<0.05, Chi-square test, SPSS ver. 7.0, USA). Culture and PCR-positive cases totalled 100, and there were 68 cases negative by both mothods. There were 79 culture-negative and PCR-positive cases, but non that were culture-positive and PCR-negative. The assay was sensitive for as little as 0.1 pg of DNA (1 bacterial cell). The specificity of detection was confirmed by ensuring that the primers did not amplify DNA extract from other bacteria. Conclusion: The PCR is rapid, accurate, and sensitive method for the detection of H. pylori.

상악 제1 및 하악 제2 소구치의 발거를 이용한 교정치료

나종열,김태우,양원식 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.1

교정치료를 위한 상악 제1 소구치, 하악 제2소구치의 발치는 한국인에서 Ⅱ급 부정교합환자가 많지 않기 때문에 그리 흔하지 않다고 생각한다. 이와 같은 발치는 다음과 같은 장점을 제공할 수 있다. 첫째, 하악 전치를 약간만 retraction을 하며, 주로 하악의 구치부를 전방이동하여 Ⅱ급 관계의 key correction을 손쉽게 할 수 있다. 둘째, 하악전치의 과도한 설측경사가 일어남을 방지한다. 이는 하악 안모의 심한 변화를 방지하여 준다. 단점으로는 하악 제1대구치의 근심경사를 들 수 있으며, 이는 적절한 mechanics의 사용으로 방지하여 줄 수 있다. 본 증례들은 Ⅱ급 1류의 특성을 가진 환자들로서 상악 제1소구치, 하악 제2소구치의 발이를 이용하여 교정치료를 하여 비교적 양호한 결과를 얻어 다음과 같이 보고하고자 한다. The purpose of this report is to present the successful improvement of occlusal relationship and facial esthetics in Class Ⅱ div. 1 malocclusion by orthodontic treatment with upper first premolars and lower second premolars extracted. Before treatment, the patients showed Class Ⅱdiv. 1 relation with severe overjet, deep overbite, large ANB angle, retrusive mandible and a convex soft tissue profile. After treatment, normal canine and molar relationships were obtained. Facial esthetics were improved. There were no mesial tipping of lower first molars and root resorptions. With the adequate diagnosis and treatment plan and biomechanics, the application of upper first and lower second premolar extraction may be one of good strategies in some Class Ⅱcases treatment.

지능형 원격 네트워크 온실

문병현,송주열,이현성,손경규,엄태환,김정우,김아름 대구대학교 정보통신연구소 2004 情報通信硏究 Vol.3 No.2

The system designed in this paper, can mark the interior state of greenhouse using sensors of temperature, humidity and illumination on greenhouse interior by greenhouse system for farm village area's agriculture automation. And, the real time monitor is possible real time monitoring by camera. Also, system that can supply water using spring cooler system and humidifier. The proposed system can sustain a designed environment of the greenhouse through rentilation fan, fluorescent light and roof door control. TCP/IP is used for the remote control of state the sensors in the client program. 본 논문에서 설계된 시스템은 농촌 지역의 농업 자동화를 위한 온실 시스템으로 온실 내부에 온도, 습도, 조도 센서를 이용한 온실 내부의 상태를 표시할 수 있으며 카메라를 이용하여 실시간 관측이 가능하다. 또한 본 시스템은 지붕 개폐, 스프링 쿨러와 가습기를 이용한 수분 공급, 환풍기, 형광등 등을 통하여 온실 내부의 환경을 유지시킬 수 있다. 모든 센서를 이용한 상태와 온실 제어를 TCP/IP를 이용한 클라이언트 프로그램에서 원격으로 관리할 수 있다.

Mycobacterium abscessus 5례

김정현,최원호,강정옥,최태열 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

단세포군 항체를 이용한 Chlamydia trachomatis의 면역형 결정

윤규석,김덕언,최태열 대한감염학회 1993 감염 Vol.25 No.1

An immunotyping of 27 strains of Chlamydia trachomatis isolated from patients with non-gonococcal urethritis or pelvic inflammatory diseases in Korea was achieved in dot-enzyme linked immunosorbent assay (dot-ELISA) with monoclonal antibodies. Monoclonal antibodies were produced with standard techniques by immunization of Balb/c mice and fusion with SP 2/0 myeloma cell. Seven type-specific (D,E,F,H,I,J,L₂), 2 subspecies-specific (B-complex, C-complex) and species-specific (HMC 1-1) monoclonal antibodies were used for immunotyping. Immunotyping of 12 control strains and 27 clinical strains isolated in Korea was studied by using dot-ELISA. Species-specific (HMC 1-1) monoclonal antibody reacted with all control strains and 27 isolated. Subspecies-specific (B-complex) monoclonal antibody reacted with B/HAR-36, Ba/Aphach-2, D/UW-3Cx, E/Bour, LGV type Ⅰ/440, LGV type Ⅱ/CDC control strains and 19 isolates. Type-specific monoclonal antibody of D was reacted with D/UW-3/Cx control strain and 10 isolates. E type-specific monoclonal antibody racted with E/Bour control strain and 6 isolates. F type-specific monoclonal antibody reacted with 5 isolates. Three isolates which racted with subspecies-specific monoclonal antibody didn't react with any type-specific monoclonal antibodies. Subspecies-specific (C-complex) monoclonal antibody reated with A/HAR-13, C/CDC, H/UW-43/Cx, J/UW-36/Cx control strains and 2 clinical isolates, but the isolates did not react with any type-specific monoclonal antibodies. One of 27 clinical isolates reacted with any type-specific monoclonal antibodies. One of 27 isolates reacted with species-specific (HMC 1-1) monoclonal antibody didn't react with any other subspecies-and type-specific monoclonal antibodies. In conclusion, the major immunotypes of C.trachomatis from urogenital system in Korea were D, E and F, and dot-ELISA with monoclonal antibody may contribute for immunotyping as a simple and specific technique.

Infrequent Restriction Site-Polymerase Chanin Reaction에 의한 Vibiro vulnificus

백은정,강정옥,최태열 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

Chlamydia trachomatis에서 분리된 Cryptic plasmid DNA의 클로닝

유정한,김덕언,박일규,강정옥,최태열 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.5

생소한 HBsAg 선별검사키트 bioelisa와 IEMA Well의 평가

김덕언,김홍석,김신규,이혜림,박일규,강정옥,최태열 대한임상검사정도관리학회 1997 臨床檢査와 精度管理 Vol.19 No.2

정상면역 환자에서 외상 후 발생한 Mycobacterium fortuitum에 의한 무릎아래주머니염 1예

박동원,김지은,백수영,박혜선,손창남,안성은,박혜정,장시형,백승삼,최충혁,최태열,배현주 대한감염학회 2008 감염과 화학요법 Vol.40 No.5

Mycobacterium fortuitum is a rare pathogen, frequently found in water, soil, animals and plant materials, It can cause infections involving skin, soft tissue and skeletal system after direct inoculation of the pathogen through surgical traumas, Punctures and injections. We report a case of infrapatellar bursitis caused by M. fortuitum in an immunocompetent, 42-year-o1d female, which occurred after bicycle trauma. She experienced marked improvement after surgical excision and debridement of the wound site and antimicrobial therapy.