위암세포주에서 Recombinant Human Interferon-r와 Adriamycin의 투여순서가 항암효과에 미치는 영향

홍원선,손영숙,김창민,강윤구,이춘택,김유철,임영혁,남현석,이진오,강태웅 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-

Numerous previous studies, both in vitro and in vivo, have demonstrated that the cytotoxicity can be enhanced by the combination of chemotherapeutic agent and interferons(IFNs) in various types of cancer cells. We have previously reported that combined treatment of MKN-45, human gastric adenocarcinoma cells, with adriamycin(ADM) and recombinant human interferon-r(rh-IFN-r) increased in the cytotoxicity. In this study, the effects of combination timing of rh-IFN-r and ADM on the cytotoxicity against MKN-45 were investigated using MTT assay. MKN-45 was treated with rh-IFN-r and ADM in vitro on three schedules : Treat A ; rh-IFN-r and ADM were treated simultaneously, Treat B ; rh-IFN-r was treated 24 hours after the treatment with ADM, Treat C ; rh-IFN-r was treated for 72 hours and followed by the treatment with ADM. The survival of MKN -45 was inhibited by ADM dose-dependently. 102 and 103U/ml of rh-IFN-r significantly inhibited the survival of MKN-45(% survival : 35.1 ±-1.2% and 34.4 ±1.1% in Treat A and 42.5 ± 2.1% and 45.9-±2.5% in Treat C, respectively). However no difference in the survival was observed between 102 and 103U/ml of rh-IFN-r. Combined treatment with rh-IFN-r and ADM significantly augmented the cytotoxicity at low concentrations of ADM. Combined effects of rh-IFN-r and ADM were evaluated using IC30(,ag/ml) to ADM. IC30s of MKN-45 in Treat A, B and C at 102 U/ml of rh -IFN-r _ were 0.019 -?- 0.003, 0.045 :I:0.001 and 0.054 ± 0.012, respectively, while IC30 of MKN-45 treated with ADM alone was 0.052±0.004. IC30s of MKN-45 in ADM alone group, Treat A, Treat B and Treat C at 103U/ml of rh-IFN-r were 0.047 ±0.003, 0.004 -±0.001, 0.031 ±0.004 and 0.056 0.008, respectively. These results indicate IC30s of Treat A and B were significantly lower than those of ADM alone(p<0.05) and IC30s of Treat A was significantly lower than those of Treat B(p <0.01). IC30s of Treat C, however, were not different from those of ADM alone. From these results demonstrating that cytotoxic effects were increased by the combination of rh-IFN-r and ADM in the order, Treat A > Treat B> Treat C, it can be concluded that the simultaneous administration of rh-IFN-r and ADM may be the most effective method to combine these two therapeutic modalties.

진행 위암환자에서 Lymphokine-Activated Killer (LAK) 활성의 저하

홍원선,김영환,송재관,강윤구,이진오,강태웅,김정룡 대한내과학회 1990 대한내과학회지 Vol.38 No.3

Natural killer(NK) and lymphokine-activated killer(LAK) activities were determined in 31 patients with unresectable stomach cancer before and immediately after chemotherapy with 5-fluorouracil, adriamycin and mitomycin C(FAM) and 31 healthy volunteers. The major purposes of the study were focused on whether peripheral blood lymphocytes(PBL) in stomach cancer patients had a similar ability in the generation of LAK activity to those in healthy volunteers and what the effect was of chemotherapy with FAM on the generation of LAK activity. LAK cells were generated in vitro by culturing human PBL with 100 U/㎖ of recombinant human interleukin-2(rH-IL-2) for 72 hours. K562(human myelogenous leukemia cell line) and MKN-45(human stomach adenocarcinoma cell line) were used as target cells for NK and LAK activities. NK activity against K562, a sensitive line, was significantly depressed in patients with stomach cancer compared with that in healthy volunteers(p<0.01). However, LAK activity against K562 was similar to that of the controls. Age, sex and performance status(ECOG 0-2 and 3-4) did not have an influence on both NK and LAK activities. LAK activity was significantly higher than NK activity, either against K562 or MKN-45, in both healthy volunteers and patients with stomach cancer(p<0.001). NK and LAK activities against MKN-45, a NK-resistant line, in patients with stomach cancer showed significantly lower levels than those in healthy volunteers. We also analyzed the effect of chemotherapy with FAM on NK and LAK activities, with no suppression of NK and LAK activities being observed. In this study, we have demonstrated that PBL of stomach cancer have a reduced ability to generate LAK activity in response to rH-IL-2. However, LAK activity generated from PBL receiving chemotherapy with FAM was similar to that of PBL without chemotherapy.

시설재배 작물의 적정 시비량 결정을 위한 간이 검정법

홍순달,임태준,강성수,양원석 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

토양의 질산태 질소 함량이 67㎎ ㎏-1에서 343 ㎎ ㎏-1의 분포를 갖는 8개 시설재배 토양에서 시비수준을 무비구와 표준시비구로 하여 오이의 생산력을 검정하고 토양 화학성과의 상호관계 및 회귀관계의 평가로부터 질소 적정 시비량 추천모델을 추정하였다. 토양의 질산태 질소 함량과 무비구 오이의 생체중, 비료효과 및 시비효율의 상호관계를 Cate & Nelson 분할법에 의하야 추정한 질소 시비의 상한기준은 280㎎ ㎏-1으로 추정되었다. 토양의 질산태 질소 수준에 따른 표준시비구의 생체중 변화의 관계로부터 토마토 질소 표준시비를 위한 질산태 질소 하한기준은 100㎎ ㎏-1으로 추정되었다. 따라서 상한기준과 하한기준 사이의 질산태 질소 검정에 의한 토마토, 오이, 배추의 질소 시비량 추천모델은 다음과 같이 개발하였다. 질소 표준시비량에 대한 비율 Y=-0.5556X +155.568. 재배기간이 장기간인 오이의 질소 덧거름 시비량 결정방법을 확립하기 위하여 ChlorophyⅡ meter (SPAD502, Minolta)를 활용한 엽록소 검정법을 검토하였다. 평가방법은 NO3-N 함량이 67∼343㎎ ㎏-1인 8개 토양에서 무비구 오이의 생체중과 경시적 엽위별 엽록소 측정치와의 회귀관계로부터 오이의 생산능력과 질소공급능력에 대한 엽록소 측정치의 기준을 추정하였다. 오이 재배기간 동안 엽위별 엽록소 측정치는 정식 후 39일째 13매 잎에서 최대를 보인 후 감소되었으며, 그 이후 오이의 엽록소 측정치는 토양의 질소 공급능력에 따라 현저한 차이를 나타냈다. 정식 후 39일째 13매엽의 엽록소 측정치는 토양의 질산태 질소, 무비구 오이의 생체중 및 질소 흡수량, 시비효율과 유의성 있는 상관관계를 보였다. 그리고 오이 최대 생체중에 대한 부족비율은 13매엽 엽록소 측정치와 유의성 있는 부의 상관을 보였으며 엽록소 최대치에 대한 부족비율과는 곡선적인 정의 상관을 보였다. 따라서 오이 최대 생체중에 대한 부족비율을 덧거름 요구량이라고 가정하면 13매엽의 엽록소 측정치는 덧거름의 사용시기와 기준을 결정할 수 있는 가능성을 보였다. 따라서 시설재배 오이의 밑거름은 질산태 질소의 토양검정에 의하여 결정하고 덧거름은 오이 정식 후 39일째 13매엽의 엽록소 측정으로 결정할 수 있는 종합적인 질소 시비추천 방안을 확립하였다.

사람폐암세포주 (PC-14)에서 Cyclosporin A에 의한 Adriamycin 내성의 극복

김영환,홍원선,송재관,강윤구,이진오,강태웅,김건열,한용철 대한내과학회 1990 대한내과학회지 Vol.38 No.3

Cyclosporin A and verapamil were tested using MTT assay to evalute the modification effect on the resistance to adriamycin in a human lung cancer cell line(PC-14) and its resistant subline(PC-14/A). PC-14/A was derived by the continuous exposure of PC-14 to incremental concentrations of adriamycin. PC-14/A was 2.5 times more resistant to adriamycin in terms of ICso than PC-14. Cyclosporin A alone, at a concentration of 2.5㎍/㎖, inhibited the growth of PC-14 to 68.3%. 2.5㎍/ ㎖ and 5.0㎍/㎖ of cyclosporin A showed an increase in the cytotoxicity of adriamycin (p<0.01) with 5.0㎍/㎖ being greater than 2.5㎍/㎖(p<0.01). Excluding the direct cytotoxic effect, however, cyclosporin A did not increase in the sensitivity of PC-14 to adriamycin but only showed an additional cytotoxic effect with adriamycin. Verapamil (up to 6.0㎍/㎖) did not inhibit the growth of PC-14. 3.0㎍/㎖ of verapamil did not increase the cytotoxic effect of adriamycin. The combination of cyclosporin A and verapamil with adriamycin enhanced the cytotoxicity of adriamycin, but the result was similar to that of cyclosporin A with adriamycin. 5.0㎍/㎖ of cyclosporin A modified the adriamycin resistance of PC-14/A(SR, 3.2). However, 3.0㎍/㎖ of verapamil did not significantly reverse the adriamycin resistance of PC-14/A. The modified effect of the combination of 5.0㎍/㎖ of cyclosporin A and 3.0㎍/㎖ of verapamil was similar to that of 5.0㎍/㎖ of cyclosporin A alone in PC-14/A. These results demonstrate that cyclosporin A has an additional cytotoxic effect with adriamycin in PC-14 and PC-14/A and has overcome the acquired resistance to adriamycin in PC-14/A. They also suggest that cyclospoin A may have the therapeutic potential in the treatment of human lung cancer.

λ-경쇄형 다발성 골수종 1예

박용관,김태원,장영,김진호,강정원,천영욱,박유환,정춘해 조선대학교 1995 The Medical Journal of Chosun University Vol.20 No.1

Multiple myeloma is a disease caused by neoplastic plasma cells that synthesize abnormal amouts of immunoglobulin or immunoglobulin fragments. Light chain myeloma are regarded as a separate category characterized by a more malignant clinical course. Light chain myelomas are said to grow fastest of all and are associated with more osteolytic lesions, more hypercalcemia, and a higher incidence of renal failure and amyloidsis than either the IgG, IgA varienties The authors experienced a case of patients with λ-light chain myeloma. A 43-year-old male patient admitted to our hospital with the chief complaint of both rib and lower back pain. The radiologic findings showed multiple pathologic fracture in ribs. osteolytic lesions in 2nd, 3rd cervical spineimmuture plasma cells. Serum electrophoresis showed normal finding. Urine electrophoresis evealed an M-spike. Urine immunoelectrophoress demonstrated λ-monoclonal protein. With the cycle of melphalan, prednisone and α-interferon chemotherapy improved of pain was observed. So we reported the case with brief review of previous literature.

Purification and Identification of Paenibacillus sp., Isolated from Diseased Larvae of Allomyrina dichotoma (Coleoptera: Scarabaeidae) in Insect Farms

Tae Hwa Kang,Sang Hun Han,Hang Yeon Weon,Young Bo Lee,Namjung Kim,Seong Hyeon Kim,Weon Tae Kim,Gwan Ho Park,Seong Hee Nam,Hae Chul Park 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

Allomyrina dichotoma is a typical pet beetles in Korea. From 2010, similar symptoms of milky spore disease were found collectively in grubs of the species reared in insect farms. They shared a specific symptom that the skin of last instar larvae was changed softer with opaque white and infested grubs eventually died. To clarify the cause of the symptom, we collected the larvae of A. dichotoma from five farms and examined intestinal bacterial florae of them using pyrosequencing technique. From those results, a member of Paenibacillus was found only in the larvae showing the symptom of disease. Through PCR analysis using a Paenibacillus specific primer set, we obtained the partial 16S rRNA gene sequence and confirmed the microbe as Paenibacillus sp. For detailed characterizing, a whole guts was extracted from each larva showing the sign of the disease and incubated at 70℃ for 15 min to isolate spore forming bacteria. After then, each content of guts was cultured on MYPGPNAL agar medium (12.5 μg/ml of nalidixic acid) at 30℃. The 16S rRNA gene sequence analysis for six isolate showed that they were closely related to P. rigui (97.5~98.0% similarity) and to P. chinjuensis (95.9~96.6% similarity). Additional tests including API test and cellular fatty acid composition analysis were performed, but the strain couldn’t be identified at species level, suggesting it may represent novel species of the genus Paenibacillus.

A Thermal Noise Measurement System for Noise Temperature Standards in <tex> $W$</tex>-Band

Tae-Weon Kang,Jeong-Hwan Kim,No-Weon Kang,Jin-Seob Kang Institute of Electrical and Electronics Engineers 2015 IEEE Transactions on Instrumentation and Measureme Vol. No.

<P>A W-band waveguide noise measurement system has been developed for noise temperature (NT) standards. The system consists of two standard noise sources and a waveguide radiometer. A horn-type cryogenic noise source operating at the liquid nitrogen temperature and an ambient temperature termination have been employed as standard noise sources. To measure noise power radiated from the noise sources including a device under test, a total-power waveguide radiometer has been constructed and its performance has been evaluated. Measured NT of a commercial waveguide noise source is presented with the measurement uncertainty of 0.10-0.13 dB (k = 2) in W-band.</P>

Correlating Fully Anechoic Chamber to Open Area Test Site Measurements by the Normalized Site Attenuation

Tae Weon Kang,Byung Wook Kim,Yeon Choon Chung,Hyo Tae Kim 한국전자파학회JEES 2001 Journal of Electromagnetic Engineering and Science Vol.1 No.2

RF and Microwave Power Standards from 10 MHz to 40 GHz over Decades

Tae-Weon Kang,Jae-Yong Kwon,Jeong-Il Park,No-Weon Kang 한국전자파학회JEES 2018 Journal of Electromagnetic Engineering and Science Vol.18 No.2

Radio frequency (RF) and microwave power is one of the key quantities in the framework of electromagnetic measurement standards. Therefore, the stability of the power standard is essential to users’ reliable measurements in various areas. Coaxial and waveguide thermistor mounts are used as transfer standards of RF and microwave power. Over decades, the effective efficiencies of thermistor mounts have been measured using coaxial and waveguide microcalorimeters in the frequency range of 10 MHz–40 GHz. The measurement uncertainty of the effective efficiency is evaluated. Results show that the power standards have been well maintained within the measurement uncertainty.

Considerations on the 1/r-Extrapolation in RE Measurements on an OATS Using a Spherical Dipole Radiator

Tae Weon Kang,Yeon Choon Chung,Byung Wook Kim,Sung Ho Won,Hyo Tae Kim 한국정보과학회 1999 Journal of Electrical Engineering and Information Vol.4 No.4

In radiated emission (RE) tests on an open area test site (OATS), measurements are often made at distances other than that relevant to the specification limit. In this case. the measured value is converted to the limit distance using a simple l/r extrapolation. However, this extrapolation is not accurate because waves reflected from the metal ground plane are present on an OATS. In this paper, we have considered the extrapolation errors using a spherical dipole radiator (SDR) by experimental and theoretical works. The theoretical extrapolation factor is determined by the analytical solution of the SDR. The field strengths at 3 m and 10 m distances have been measured to obtain experimental extrapolation factors. The results show that the 1/r extrapolation introduces measurement uncertainty of -4.86~+0.44 ㏈ for horizontal polarization and -9.16~-0.06 ㏈ for vertical polarization.