그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력

최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1

An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.

중합효소연쇄반응을 이용한 Helicobacter pylori검출

최태열,박경남,강정옥,서일혜 대한감염학회 1997 감염 Vol.29 No.5

배 경 : H.pylori는 위염, 위궤양 재발과 밀접한 관계를 갖고 있기 때문에 검사실적 진단은 정확하여야 한다. H. pylori의 세균배양이 가장 바람직하지만 시설이나 노력면에서 많은 어려움이 있다. 이에 저자는 H. pylori의 세균배양과 더불어 PCR 을 실시하여 PCR의 유용성을 판단코져 다음과 같은 실험을 실시 하였다. 방 법 : 위장 장애를 호소하여 위내시경과 생검을 실시한 247명의 병리조직소견에 따라 정상대조군(57명), 만성위염(131명), 활동성만성위염(19명), 만성궤양(8명), 위암(32명) 으로 분류하였다. 세균배양은 brain heart infusion egg yolk agar선택배지를 사용하였고 PCR은 usease A gene sequence 를 증폭할수 있는 primer 2쌍을 선택하여 nested PCR 을 실시하였다. 결 과 : 세균배양에 의한 H. pylori의 검출율은 100명(40%) 였으며, PCR 에 의한 H.pylori의 검출율은 179명(72%) 으로 PCR 법이 세균배양법보다 검출율이 높았다(P:<0.05, Chi-square test, SPSS, ver7.0, USA). 세균배양과 PCR 모두 음성이 68명, 모두양성이 100명, 세균배양음성 PCR양성이 79명이였으며 PCR의 예민도는 0.1pg DNA(1 bacterial cell)였다. 일반세균을 이용한 특이도 검사에서 양성인 예는 없었다. 결 론 : 상기 결과로 미루어 보아 위생검조직에서 H.pylori의 검출은 연구자들이 사용한 PCR 법이 세균배양법보다 신속 정확하였다. Background : Helicobacter pyloir has been implicated in the pathogenesis of active chronic gastritis and peptic ulcer disease in man. Thus, diagnosis and treatment of H. pylori infection are now of growing importance in ucle management. A variety of noninvasive and invasive methods have been described for the detection of H. pylori, but all of these techniques have disadvantages such as time consuming or insensitivity. So we describe the polymerase chain reaction(PCR) assay for the sensitive and specific detection of H. pylori. Methods : Gastric biopsy specimens were obtained from 147 patients undergoing endoscopic examinations at Hayang University Hospital. One half of the specimen was processed for routine culture and the other half for PCR. Bacterial genomic DNA from gastric biopsies are extracted by Instagene. Two sets of primer pairs derived from the nucleotide sequence of the urease A gene of H. pylori were used. Result: H. pylori was cultured in 100(40%) cases and PCR assay deteted 179(72%) cases (P<0.05, Chi-square test, SPSS ver. 7.0, USA). Culture and PCR-positive cases totalled 100, and there were 68 cases negative by both mothods. There were 79 culture-negative and PCR-positive cases, but non that were culture-positive and PCR-negative. The assay was sensitive for as little as 0.1 pg of DNA (1 bacterial cell). The specificity of detection was confirmed by ensuring that the primers did not amplify DNA extract from other bacteria. Conclusion: The PCR is rapid, accurate, and sensitive method for the detection of H. pylori.

단세포군 항체를 이용한 Chlamydia trachomatis의 면역형 결정

윤규석,김덕언,최태열 대한감염학회 1993 감염 Vol.25 No.1

An immunotyping of 27 strains of Chlamydia trachomatis isolated from patients with non-gonococcal urethritis or pelvic inflammatory diseases in Korea was achieved in dot-enzyme linked immunosorbent assay (dot-ELISA) with monoclonal antibodies. Monoclonal antibodies were produced with standard techniques by immunization of Balb/c mice and fusion with SP 2/0 myeloma cell. Seven type-specific (D,E,F,H,I,J,L₂), 2 subspecies-specific (B-complex, C-complex) and species-specific (HMC 1-1) monoclonal antibodies were used for immunotyping. Immunotyping of 12 control strains and 27 clinical strains isolated in Korea was studied by using dot-ELISA. Species-specific (HMC 1-1) monoclonal antibody reacted with all control strains and 27 isolated. Subspecies-specific (B-complex) monoclonal antibody reacted with B/HAR-36, Ba/Aphach-2, D/UW-3Cx, E/Bour, LGV type Ⅰ/440, LGV type Ⅱ/CDC control strains and 19 isolates. Type-specific monoclonal antibody of D was reacted with D/UW-3/Cx control strain and 10 isolates. E type-specific monoclonal antibody racted with E/Bour control strain and 6 isolates. F type-specific monoclonal antibody reacted with 5 isolates. Three isolates which racted with subspecies-specific monoclonal antibody didn't react with any type-specific monoclonal antibodies. Subspecies-specific (C-complex) monoclonal antibody reated with A/HAR-13, C/CDC, H/UW-43/Cx, J/UW-36/Cx control strains and 2 clinical isolates, but the isolates did not react with any type-specific monoclonal antibodies. One of 27 clinical isolates reacted with any type-specific monoclonal antibodies. One of 27 isolates reacted with species-specific (HMC 1-1) monoclonal antibody didn't react with any other subspecies-and type-specific monoclonal antibodies. In conclusion, the major immunotypes of C.trachomatis from urogenital system in Korea were D, E and F, and dot-ELISA with monoclonal antibody may contribute for immunotyping as a simple and specific technique.

Infrequent Restriction Site-Polymerase Chanin Reaction에 의한 Vibiro vulnificus

백은정,강정옥,최태열 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

Mycobacterium abscessus 5례

김정현,최원호,강정옥,최태열 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

Chlamydia trachomatis에서 분리된 Cryptic plasmid DNA의 클로닝

유정한,김덕언,박일규,강정옥,최태열 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.5

생소한 HBsAg 선별검사키트 bioelisa와 IEMA Well의 평가

김덕언,김홍석,김신규,이혜림,박일규,강정옥,최태열 대한임상검사정도관리학회 1997 臨床檢査와 精度管理 Vol.19 No.2

정상면역 환자에서 외상 후 발생한 Mycobacterium fortuitum에 의한 무릎아래주머니염 1예

박동원,김지은,백수영,박혜선,손창남,안성은,박혜정,장시형,백승삼,최충혁,최태열,배현주 대한감염학회 2008 감염과 화학요법 Vol.40 No.5

Mycobacterium fortuitum is a rare pathogen, frequently found in water, soil, animals and plant materials, It can cause infections involving skin, soft tissue and skeletal system after direct inoculation of the pathogen through surgical traumas, Punctures and injections. We report a case of infrapatellar bursitis caused by M. fortuitum in an immunocompetent, 42-year-o1d female, which occurred after bicycle trauma. She experienced marked improvement after surgical excision and debridement of the wound site and antimicrobial therapy.

Chlamydia trachomatis진단에 유용한 단세포군 항체 생산에 관한 연구

최태열(Tae-Yeal Choi),김신규(Think You Kim),김춘원(Choon Won Kim),김기홍(Ki-Hong Kim),황응수(Eung-Soo Hwang),차창룡(Chang-Yong Cha),김광혁(Kwang-Hyuk Kim) 大韓微生物學會 1987 大韓微生物學會誌 Vol.22 No.3

HIV 검사

최태열 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.2

Detection of HIV antibodies is still the most efficient and most common way to determine whether an individual has been exposed to HIV and to screen blood and blood products for this infections agent. A test is considered positive when assay such as the enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA), and western blots (immunoblots)are considered reactive. A positive test result indicates exposure and, outside of the perinatal and neonatal period, is presumed to indicate infection by teh virus. The ELISA is the most commonly used to screen for HIV because of its relatively low cost, standardized procedure, high reliability, and ability to give rapid result. Under optimal laboratory conditions, the sensitivity and specificity in most cases are>99%. The Western blot assay is the test most commonly used for confirming the presence of HIV-specific antibodies. However, western blots are more expensive and more time consuming than ELISAs, and they require more technical expertise owing to subjective interpretations, Specificity and sensitivity are high but are some what dependent on the interpretive criteria employed. A definitive test for an active infection is the recovery of HIV from cells or cell-free fluids of the infected individual by using the solid-phase capture technique, indirect immunofluorescence assay, ploymerase chain reaction... etc. Surrogate markers (CD4+ -CEll count, P24 antigen in plasma, 2-microglobulin... etc.) are not only important in predicting the out come of HIV infection but also useful in determining when treatment should be administered. Inconclusion, while awaiting the development of more-effective antiviral therapies, early testing for HIV in potentially infected individuals is recommended to allow prompt medical treatment and surveillance and to prevent the spread of the virus to other individuals.