비임균성 뇨도염 환자에서 Chlamydia trachomatis 검출방법에 관한 연구 (배양법, 효소면역법 및 직접면역형광법의 비교 관찰)

최태열,김춘원,김중환,Choi, Tae-Yeal,Kim, Choon-Won,Kim, Jung-Hwan 대한미생물학회 1986 大韓微生物學會誌 Vol.21 No.3

Chlamydia trachomatis is one of the most common cause in non-gonococcal urethritis. There are several diagnostic methods for Chlamydia trachomatis; culture method using McCoy cell, enzyme immunoassay and direct immunofluorescence staining etc. We have studied a sensitivities of culture, chlamydiazyme and direct immunofluorescence staining(DIF). 85 male patients previously conformed to non-gonococcal urethritis have been selected in this study. Three samples were concurrently collected in the same patient. First sample was used to inoculation in McCoy cell, 2'nd sample was used to Chlamydiazyme test and 3'rd sample was used to direct immunofluorescence staining method. The results are following. 1. All culture, Chlamydiazyme and DIF positive cases are 15/85(17.7%). 2. Culture and Chlamydiazyme positive but DIF negative cases absent. 3. Culture and DIF positive, but Chlamydiazyme negative cases are 2/85(2.4%). 4. Chlamydiazyme and DIF positive, but culture negative cases are 9/85(10.6%). 5. Culture positive, but Chlamydiazyme and DIF negative cases are 6/85(7.1%). 6. Chlamydiazyme positive, but culture and DIF negative cases are 7/85(8.2%). 7. DIF positive, but culture and Chlamydiazyme negative cases are 3/85(3.5%). 8. All culture, Chlamydiazyme and DIF negative cases are 43/85(50.1%). In summarized, anyone positive cases of culture, Chlamydiazymc and DIF are 42/85(49.9%).

Chlamydia trachomatis진단에 유용한 단세포군 항체 생산에 관한 연구

최태열(Tae-Yeal Choi),김신규(Think You Kim),김춘원(Choon Won Kim),김기홍(Ki-Hong Kim),황응수(Eung-Soo Hwang),차창룡(Chang-Yong Cha),김광혁(Kwang-Hyuk Kim) 大韓微生物學會 1987 大韓微生物學會誌 Vol.22 No.3

Morganella morganii에 의해 유발되었다고 사료되는 피부 궤양

권상진,이창우,최태열 ( Sang Jin Kwon,Chang Woo Lee,Tae Yeal Choi ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.5

Morganella morganii, a gram, negative rod is often regarded as an opportunistic, secondary invader rather than a primary pathogen on the skin. It has been isolated from blood, sputa, and pus from patients with respiratory tract and wound infections or with bacteremia. A 2-year-old boy presented with erythematous ulcerative lesions on the cheeks and left knee which had a tendency to superficial scarring. The organism isolated from the ulcer displayed a biochemical char acteristics typical of Morganella morganii. The lesions responded well to systemic antibiotic therapy with amikacin and carbenicillin, which were recognized as effective drugs in in vitro sensitivity testing. (Korean J Dermatol 19S7;35(5): 1018-1021)

중합효소연쇄반응을 이용한 Chlamydia trachomatis의 검출

서일혜(Yiel Hea Seo),최태열(Tae Yeal Choi) 大韓微生物學會 1995 大韓微生物學會誌 Vol.30 No.2

Nested Polymerase Chain Reaction을 이용한 연령 및 감염부위별 단순포진 바이러스 아형 분류

허환 ( Hwan Herr ),김영태 ( Young Tae Kim ),최태열 ( Tae Yeal Choi ) 대한피부과학회 1998 大韓皮膚科學會誌 Vol.36 No.4

Breakdown of the Vaginal Ecosystem in Patients with rheumatoid arthritis

심승철(Seung Cheol Shim),김태환(Tae Hwan Kim),전재범(Jae Bum Jun),정성수(Sung Soo Jung),이인홍(In Hong Lee),배상철(Sang Cheol Bae),황정혜(Jung Hye Hwang),문영진(Young Jin Moon),조수현(Soo Hyun Cho),최태열(Tae Yeal Choi),유대현(Dae Hy 대한내과학회 1998 대한내과학회 추계학술대회 Vol.55 No.-

사염화에틸렌에 의한 병아리배자의 심장기형 및 기능변화에 관한 연구

김 완(Wan Kim),김원규(Won-Kyu Kim),최태열(Tae-Yeal Choi),조자연(Ja-Yeon Cho) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2

본 연구는 사염화에틸렌 (tetrachloroethylene)을 병아리 배자에 투여하여 심혈관기형과의 유관성을 조사하였다. 또한 투여 후 초기 변화로써 세포자사와 연관된 세포손상 및 혈류량 감소와 같은 생리기능적 장애등을 조사하여, 심혈관기형의 형태발생학적 기전에의 접근을 시도하고자 하였다. Hamburger-Hamilton 발생 27기 및 28기의 수정된 계란을 90~96시간 배양하여 실험군에는 40 μM, 50 μM의 두가지 농도의 사염화에틸렌을 mineral oil 0.05 ml에 용해시켜 융모요막에 주입하였고, 대조군은 식염수, mineral oil군으로 나누어 각각 0.05 ml의 식염수와 mineral oil을 투여하였다. 사염화에틸렌 주입 후 6분에서 30분간 심전도 변화를 추적하였고, pulse doppler velocity meter로 배대동맥 (dorsal aorta)을 통해 혈류의 속도를 측정하였으며, 실험군과 대조군의 14일 배자들을 고정하고 조직표본을 제작한 후 광학현미경으로 관찰하였고, 전자현미경조직표본을 제작하여 투과전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 40~50 μM 농도에서 심혈관 기형이 가장 잘 유발되어, 여러 가지 형태의 심장기형이 실험군에서 통계적으로 유의하게 (p<0.01) 관찰되었고, 상당한 치사율을 동반하였다. 심장기형으로는 대형심실사이막결손, 대형심방사이막결손, 심장속막융기 결손, 양대혈관 오른심실기시 (double outlet right ventricle)와 동맥줄기개존을 동반한 단심실이었으며, 이중 대형심실사이막결손이 실험군 410예중 48예 (11.7%)로 가장 많았다. 심전도상에선 투여 6분에서 30분 후에 심실세동을 포함한 부정맥이 보였고, 도플러 (doppler) 측정상 심전도 이상과 일치하여 배대동맥의 혈류량 감소가 두드러졌다. 광학현미경 소견상 개별 세포사를 주 소견으로 하는 대량의 세포괴사를 보였으며, 전자현미경적소견상 배자심근 (embryonic myocardium)의 세포손상을 나타내는 소견이 관찰되었다. Chlorinated solvents are recognized as well-known drinking water contaminants and potent teratogens for developing embryos. Among theses compounds, tetrachloroethylene was studied to evaluate the teratogenic effects in detail. This study was also undertaken to understand the teratogenetic mechanisms by assessing the relationship between the physiological impairments and cellular degeneration including apoptosis induced by tetrachloroethylene, and the cardiovascular malformations. Fertilized white leghorn eggs (n=530) were incubated for 90~96 hours up to Hamburger-Hamilton stage 27~28. To the experimental group, tetrachloroethylene was dissolved in mineral oil at concentrations of 40 μM and 50 μM and was injected into the chorioallantoic membrane. One half of the control was injected with saline and the other half with mineral oil. The experimental animals are studied for ECG changes, and light and electron microscopic observation. Two silver thread electrodes were used to record the ECG. ECG changes were recorded for 6~30 minutes after tetrachloroethylene injections. In parallel studies, blood velocity through the dorsal aorta was measured by a pulse Doppler velocity meter with 20 MHz probe. The specimens were stained with hematoxylin and eosin for light microscopic study and the Hitachi H-800 EM was used for transmission electron microscopic evaluation. The embryos underwent formalin fixation on day 14, and the presence of malformations was observed by a microscopy. The results obtained are as follows: Repeated injections of 40~50 μM tetrachloroethylene appeared to have a strong influence on the formation of cardiovascular malformations. Various types of cardiac malformations were observed in the experimental group compared to control group, that were statistically significant (p<0.01) and many embryos also died. The malformations observed were large ventricular septal defect, endocardial cushion defect, double outlet right ventricle and truncus arteriosus associated with single ventricle. Large ventricular septal defect was most common type of cardiovascular abnormalities. Arrythymias including ventricular fibrillations were evident on ECG 6~30 minutes after the injections. Blood flow through the dorsal aorta was markedly decreased in concordance with the ECG changes. A large amount of individual cell death suggesting apoptosis was seen on light microscopic evaluation and the electron microscope revealed cellular degeneration of the embryonic myocardium. We concluded that tetrachloroethylene, like other chloride compounds has potent teratogenic effects. The cardiac malformations were probably caused by decreased blood flow due to physiofunctional changes and cellular necrosis associated with apoptosis. By including birds and mammals in further experiments, we hope to elucidate an embryological clue into how early cardiovascular malformations are caused.

임상 ; 적극적 감염 관리를 통한 신생아 중환자실내의 MRSA 근절 효과: 격리 프로그램 및 Chlorhexidine 사용

김형진 ( Hyoung Jin Kim ),강성진 ( Sung Jin Kang ),박현경 ( Hyun Kyung Park ),김창렬 ( Chang Ryul Kim ),최태열 ( Tae Yeal Choi ),오성희 ( Sung Hee Oh ) 대한주산의학회 2010 Perinatology Vol.21 No.3

목적: 신생아중환자실에서 MRSA 집락화 및 균혈증을 줄이기 위한 노력으로 격리 프로그램과 2% CHG 사용 효과를 알아보고자 하였다. 방법: 2007년 6월부터 2009년 10월까지 한양대학교 서울병원 신생아중환자실에 입원한 414명의 환아를 대상으로 의무 기록을 후향적으로 조사하였다. 2007년 6월부터 2007년 10월까지를 1기, 2007년 11월부터 2008년 10월까지 격리 프로그램을 시행한 기간을 2기, 2008년 11월부터 2009년 10월까지 격리 프로그램과 더불어 피부소독제를 2% CHG으로 바꾸어 사용한 기간을 3기로 하였다. 전 기간 내내 MRSA 감시 배양(MRSA surveillance culture)을 시행하였다. 결과: MRSA 보균 및 감염의 위험인자로 알려진 출생 체중, 조산아, 입원 기간, 중심혈관 카테터 삽입 기간 등에서 3군간 유의한 차이를 보이는 요인이 없었으나, 격리 프로그램 및 2% CHG 사용에 따라 MRSA 집락화 및 균혈증 발생률이 통계학적으로 의미 있게 감소하였다. MRSA 집락화 및 균혈증의 위험 인자 조사에서는 입원기간, 중심혈관 삽입 여부 및 기간, 신생아 호흡 곤란 증후군 진단받은 경우, 기관지폐 형성이상 진단받은 경우에서 위험성이 더 높은 것으로 나타났고 격리 프로그램과 CHG 사용을 시행한 경우 위험성이 감소하는 것으로 나타났다. 결론: 신생아 중환자실에서 MRSA 보균 및 감염률을 낮추기 위해서는 철저한 손 씻기와 적극적인 MRSA 배양검사를 하면서, 적극적인 격리 관리와 피부 소독제로 CHG를 사용하는 것을 고려해 보아야 하며, 중심혈관 삽입기간을 최소화하는 노력도 해야 한다. Purpose: The increasing incidence and mortality of Methicillin-resistant Staphylococcus aureus (MRSA) colonization or blood-stream infection is an important problem in neonatal intensive care unit (NICU). The aims of this study are to evaluate the effective eradication of MRSA through the aggressive isolation program with or without the use of 2% chlorhexidine-gluconate (CHG) and to investigate significant risk factors of MRSA colonization in NICU. Methods: This study is a retrospective collected data among 414 neonates admitted to a NICU from June 1, 2007, through October 31, 2009. We divided the groups into 3 periods according to isolation program or the use of 2% CHG. Results: The aggressive isolation program decreased the incidence of MRSA colonization and the additional use of 2% CHG has reduced much more the incidence of MRSA colonization and bacteremia. Days of hospitalization, use of central line, days of using central line, presence of respiratory distress syndrome (RDS) or bronchopulmonary dysplasia (BPD), isolation program, and isolation program + use of CHG were significant factors associated with MRSA colonization or bacteremia in univariate logistic regression analysis. Days of using central line and isolation program + use of CHG were significant after in multivariate logistic regression analysis. Conclusion: Hand hygiene, active MRSA surveillance culture, isolation, contact isolation, nursing/doctor cohorts and the use of 2% CHG as skin sterilizer were effective in eradicating to MRSA. The effort of shortening the days of using central line is also necessary.

혈청 고 Alkaline Phosphatase 활성도를 보인 각종 질환 52 예의 Alkaline Phosphatase 의 Isoenzyme Study

오현관 ( Hyun Kwan Oh ),박승일 ( Seung Il Park ),홍순표 ( Soon Pyo Hong ),박상호 ( Sang Ho Park ),강준구 ( Jun Koo Kang ),서영환 ( Young Whan Seo ),최태열 ( Tae Yeal Choi ) 대한내과학회 1984 대한내과학회지 Vol.27 No.5

단세포군 항체를 이용한 Chlamydia trachomatis의 면역형 결정

윤규석,김덕언,최태열 대한감염학회 1993 감염 Vol.25 No.1

An immunotyping of 27 strains of Chlamydia trachomatis isolated from patients with non-gonococcal urethritis or pelvic inflammatory diseases in Korea was achieved in dot-enzyme linked immunosorbent assay (dot-ELISA) with monoclonal antibodies. Monoclonal antibodies were produced with standard techniques by immunization of Balb/c mice and fusion with SP 2/0 myeloma cell. Seven type-specific (D,E,F,H,I,J,L₂), 2 subspecies-specific (B-complex, C-complex) and species-specific (HMC 1-1) monoclonal antibodies were used for immunotyping. Immunotyping of 12 control strains and 27 clinical strains isolated in Korea was studied by using dot-ELISA. Species-specific (HMC 1-1) monoclonal antibody reacted with all control strains and 27 isolated. Subspecies-specific (B-complex) monoclonal antibody reacted with B/HAR-36, Ba/Aphach-2, D/UW-3Cx, E/Bour, LGV type Ⅰ/440, LGV type Ⅱ/CDC control strains and 19 isolates. Type-specific monoclonal antibody of D was reacted with D/UW-3/Cx control strain and 10 isolates. E type-specific monoclonal antibody racted with E/Bour control strain and 6 isolates. F type-specific monoclonal antibody reacted with 5 isolates. Three isolates which racted with subspecies-specific monoclonal antibody didn't react with any type-specific monoclonal antibodies. Subspecies-specific (C-complex) monoclonal antibody reated with A/HAR-13, C/CDC, H/UW-43/Cx, J/UW-36/Cx control strains and 2 clinical isolates, but the isolates did not react with any type-specific monoclonal antibodies. One of 27 clinical isolates reacted with any type-specific monoclonal antibodies. One of 27 isolates reacted with species-specific (HMC 1-1) monoclonal antibody didn't react with any other subspecies-and type-specific monoclonal antibodies. In conclusion, the major immunotypes of C.trachomatis from urogenital system in Korea were D, E and F, and dot-ELISA with monoclonal antibody may contribute for immunotyping as a simple and specific technique.