갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징

오병권,홍경애,송성준,유장걸,이선주,이영일 제주대학교 방사능이용연구소 2003 연구보고 Vol.17 No.-

제주도에서 자생하는 갯쑥부정이(Heteropappus hispidus(Thunb.))에 감마선을 10, 20, 40, 80, 120, 160, 200, 300, 400Gy 수준으로 나누어서 조사한 뒤 발아율 및 발아 30일 후의 본엽 출현을, LD_(50), 본엽길이, 줄기부분의 안토시아닌 색소 형성 유무, 생육 60일 후의 로젯형 엽, multi-shoot 형성율, 초장, 채화 결실율등의 주요 양적형질들을 생존개체를 대상으로 실시하였다. 120Gy 까지의 발아율은 88.5%로 대조구와 큰 차이를 보이지 않았으나 160Gy 이상에서 부터는 급격히 감소하여 치사선량(LD_(50))은 160Gy 범위 정도였다. 또한, 본엽 출연율과 그 길이는 조사선량이 높아질수록 억제되었다. 안토시아닌 색소 형성은 조사전량이 높을수록 대조구 2%에서 30%로 높아졌으며 로젯형도 고선량 처리구에서 발생하였다. Multi-shooting은 80Gy 이상일 때 부터 나타났으며 multi-shooting을 형성하는 개체들은 초장은 짧고 줄기는 가늘게 다발형으로 자라는 특성이 있어서 만일 형질이 고정된다면 화분 관상용 개체로 선발하기에 적합하였다. 특히, 조사선량이 증가됨에 따라서 안토시아닌 색소 형성, 로젯형 개체, multi-shoot등의 개체가 많이 출현되었는데 이는 돌연변이 유기의 선발지표가 될 수 있을 것으로 보인다. The seeds of naturally growing Heteropappus hispidus Thunb were treated by nine different doses(0, 10, 20, 40, 80, 120, 160, 200, 300, 400㏉) of gamma rays to investigate their germination rate and to quantify the characteristics of their germinated plant as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120 ㏉ was not greatly affected but was rapidly decreased at over 160 ㏉. It seemed that lethal dose(LD_(50)) of germination was 160 ㏉. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40 ㏉. Multi-shoots were developed over 80 ㏉. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the morphologically markers for the mutant selection of Heteropappus hispidus (Thunb.)

갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징

오병권,홍경애,송성준,유장걸,이선주,이영일 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

제주도에서 자생하는 갯쑥부쟁이(Heteropappus hispidus (Thunb.))에 감마선을 10, 20, 40, 80, 120, 160, 200, 300, 400 Gy 수준으로 나누어서 조사한 뒤 발아율 및 발아 30일 후의 본엽 출현율, LD_(50), 본엽길이, 줄기부분의 안토시아닌 색소 형성 유무, 생육 60일 후의 로젯형 엽, multi-shoot 형성율, 초장, 개화 결실률 등의 주요 양적형질들을 생존개체를 대상으로 실시하였다. 120 Gy 까지의 발아율은 88.5%로 대조구와 큰 차이를 보이지 않았으나 160 Gy 이상에서 부터는 급격히 감소하여 치사선량(LD_(50))은 160 Gy 범위 정도였다. 또한, 본엽 출현율과 그 길이는 조사선량이 높아질수록 억제되었다. 안토시아닌 색소 형성은 조사선량이 높을수록 대조구 2%에서 30%로 높아졌으며 로젯형도 고선량 처리구에서 발생하였다. Multi-shooting은 80 Gy 이상일 때 부터 나타났으며 multi-shooting을 형성하는 개체들은 초장은 짧고 줄기는 가늘게 다발형으로 자라는 특성이 있어서 만일 형질이 고정된다면 화분 관상용 개체로 선발하기에 적합하였다. 특히, 조사선량이 증가됨에 따라서 안토시아닌 색소 형성, 로젯형 개체, multi-shoot등의 개체가 많이 출현되었는데 이는 돌연변이 유기의 선발지표가 될 수 있을 것으로 보인다. The seeds of naturally growing Heteropappus hispidus (Thunb.) were treated by nine different doses (0, 10, 20, 40, 80, 120, 160, 200, 300, 400 ㏉) of gamma rays to investigate their germination rate and to quantify the characteristics of their germinated plants as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120 ㏉ was not greatly affected but was rapidly decreased at over 160 ㏉. It seemed that lethal dose (LD_(50)) of germination was 160 ㏉. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40 ㏉. Multi-shoots were developed over 80 ㏉. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the high dose of gamma rays could be utilized as the morphological markers for the mutant selection of Heteropappus hispidus (Thunb.).

두개내 고압과 두개내 저압을 교대로 보인 두통 환자 1예

정선주,권순억,김종성 대한두통연구회 2002 두통 Vol.3 No.2

임신여성을 제외한 성인에서의 B군 연쇄구균 감염에 관한 임상적 고찰

고경식,권선희,안우석,이원욱,김구엽,서환조 대한감염학회 1995 감염 Vol.27 No.4

목적:B군 연쇄구균은 최근 보고에 의하면 만성 기저질환(당뇨병, 악성종양등)이 있는 성인에서 감염빈도 및 사망률, 이환율이 증가하고 있는 추세이며 그 중요성이 대두되고 있다. 이에 저자들은 B군 연쇄구균 감염의 임상양상, 기저질환(위험인자)을 알아보고, B군 연쇄구균 항균제 감수성등을 규명하고자 하였다. 방법:1986년 1월부터 1993년 12월까지 경희대학교 부속병원에서 B군 연쇄구균이 배양된 274예를 대상으로 의무기록지를 검토하였다. 결과: 1) 전체 연구대상은 274예로 98예(35.8%)가 남자였고, 176예(64.2%)가 여자였다. 이들의 연령분포를 보면 신생아군에서 감염이 많이 발생하였다 이후 감소추세를 보이다가 20대 이상에서 다시 증가 하는 추세를 보였다. 2) 감염 양상은 274명중 명확한 감염이 156명(56.9%), 추정감염이 55명(20.1%), 비감염이 63명(23.0%)이었다. 임신을 제외한 18세 이상의 성인은 231명으로 남자가 83명, 여자가 148명이었고 남여 비율은 1:1.78이었다. 이중 133예(57.6%)에서 명확한 감염이었고, 46명(19.9%)에서 추정 감염, 52명(22.5%)에서 비감염이었다. 명확한 감염환자 133명중 31명(23.3%)이 병원내 감염이였다. 3) 18세 이상의 성인 231명에서 Group B Streptococcus가 분리 동정된 감염 부위는 자궁 경부 77예(33.3%), 요 73예(31.6%), 객담및 상기도 39예(16.9%), 농 24예(10.4%)등이었다. 4) 명확한 감염을 가지고 있었던 성인 133명의 임상양상은 요로 감염 37명(28%), 자궁 경부염 37명(28%), 피부, 연조직 감염 23명(17%), 폐렴 10명(8%), 패혈증 8명(6%)등이었다. 5) 명확한 감염을 가지고 있었던 성인 133명중 92명(69%)에서 한가지 이상의 만성 기저질환이 있었으며, 이중 당뇨병 32%, 뇌 혈관 질환 21%, 고혈압 19%, 신부전 12%, 악성종양 5%등을 차지하였다. 6) 항균제 감수성 검사(251예)에서 penicillin 98.4%, cephalothin 99.6%, cefamandol 98.8%, erythromycin 96.0%, clindamycin 89.4%, oxacillin 88.8%, lincomycin 87.8%, methicillin 86.8%, minocycline 49.4%, trimethoprim-sulfamethoxazole 15.5%의 감수성을 보였다. 7) 명확한 성인 감염 133명중 6명(4.5%)이 사망하였다. 결론:B군 연쇄구균은 만성 기저질환이 있는 성인에서 감염 빈도가 높고, 치명적인 결과를 초래할수 있으므로 감염 예방 및 조기 발견, 적절한 항균제 감수성 검사에 따라 조기 치료로 사망률을 줄여야 할것으로 생각된다. Background: Several reports have suggested that the incidence of diseases caused by Group B streptococcus is increasing in nonpregnant adults, particularly those with serious underlying diseases. The objectives of this study were to define the clinical manifestations, underlying conditions(risk factors) of definite group B streptococcal diseases in nonpregnant adults and to obtain antimicrobial sensitivity of these infections. Methods: We analyzed the records of 274 cases of the Group B streptococcus isolated in Kyung-Hee University hospital between 1986 and 1993. Results: 1) Total Group B streptococcal isolates were 274 cases : Of these cases, 7 percent were 1 month of age or younger, 3 percent were older than 1 month but younger than 18 years of age, and 91 percent were 18 or older. 2) Men and nonpregnant women accounted for 87 percent (n=231) : 133 cases (57.6%) with definite infection, 46 cases(19.9%) with possible infection, and 52 cases(22.5%) with no infection. 31 cases of 133 (23.3%) nonpregnant adult with definite infection apeared to have acquired the disease nosocomially. 3) In the 133 nonpregnant adult patients, the most common clinical diagnosis were urinary tract infection(28%), and cervicitis(28%). 4) In the 133 nonpregnant adult patients, the underlying diseases or conditions were diabetes mellitus(32%), cerebrovascular accident(21%), hypertension(19%), renal failure(12%), and malignant neoplasm(5%), Sixty-nine percent of the cases had one or more underlying diseases. 5) In the antimicrobial sensitivity tests, 98.4% of isolates were sensitive to penicillin, 99.6% to cephalothin, 98,8% to cefamandol, 96.0% to erythromycin, 89.4% to clindamycin, and 49.4% to minocycline. 6) The case mortality rate was 4.5%(6 of 133). Conclusion:We found group B streptococcal infection to be a significant and apparently increasing cause of definite diseases in nonprognant adults, particularly in those with serious underlying diseases. Recognizing these infections early in the groups with serious underlying diseases will be helpful in lowering mortality.

Efficacy and Safety of Combination Antiplatelet Therapies in Patients With Symptomatic Intracranial Atherosclerotic Stenosis

Kwon, Sun U.,Hong, Keun-Sik,Kang, Dong-Wha,Park, Jong-Moo,Lee, Ju-Hun,Cho, Yong-Jin,Yu, Kyung-Ho,Koo, Ja-Seong,Wong, K.S. Lawrence,Lee, Seung-Hoon,Lee, Kyung Bok,Kim, Dong-Eog,Jeong, Sang-Wook,Bae, He Ovid Technologies Wolters Kluwer -American Heart A 2011 Stroke Vol.42 No.10

<P>An optimal strategy for management of symptomatic intracranial atherosclerotic stenosis (ICAS) has not yet been established. We compared the efficacy of 2 combinations of antiplatelets, aspirin plus cilostazol (cilostazol group) verus aspirin plus clopidogrel (clopidogrel group), on the progression of ICAS, which is known to be associated with clinical stroke recurrence.</P>

포스터 발표 : Ethambutol에 의해 유발된 급성 신부전 1예

권순효 ( Kwon Sun Hyo ),김정훈 ( Kim Jeong Hun ),이준영 ( Lee Jun Yeong ),박진우 ( Park Jin U ),길효욱 ( Gil Hyo Ug ),양종오 ( Yang Jong O ),이은영 ( Lee Eun Yeong ),이현순 ( Lee Hyeon Sun ),홍세용 ( Hong Se Yong ) 대한신장학회 2003 춘계학술대회 초록집 Vol.22 No.1

The Thickness and Texture of Temporal Bone in Brain CT Predict Acoustic Window Failure of Transcranial Doppler

Kwon, Jee-Hyun,Kim, Jong S.,Kang, Dong-Wha,Bae, Kyun-Seop,Kwon, Sun U. LITTLE, BROWN AND COMPANY, MEDICAL JOURNALS 2006 JOURNAL OF NEUROIMAGING Vol.16 No.4

<P>BACKGROUND AND PURPOSE: Although transcranial Doppler (TCD) is useful for evaluation and treatment of ischemic stroke, temporal acoustic window failure (TAWF) limits its application. We performed this study to reveal whether initial brain CT of acute stroke patients predicted TAWF. METHODS: We performed TCD in 92 acute ischemic stroke patients (57 males, aged 38-84 years) with brain CT scans. We measured the thickness (BTW) and evaluated the texture of the temporal bone in acoustic windows shown in the CT scan with the use of picture archiving and communication system. The bone mineral density (BMD) of the vertebral bodies, femurs, and whole body was also measured. RESULTS: Doppler signals were adequately obtained in 121 (65.8%) of the 184 middle cerebral arteries (MCA) from both cerebral hemispheres. BTW and inhomogeneity of temporal bone, besides age, sex, and hypercholesterolemia, was strongly correlated with TAWF. BMD was not directly correlated with TAWF, but inhomogeneous temporal bone was correlated with lower BMD scores. If the brain CT scan showed thick (BTW >or= 2.7 mm) and inhomogeneous temporal bone, the possibility of TAWF was 93.5%, while if brain CT scan showed thin (BTW < 2.7 mm) and homogenous temporal bone, it was only 5.4%. CONCLUSIONS: The texture as well as the thickness of the temporal bone was the most important determinant of TAWF. When acute stroke patients had thin and homogeneous temporal bones in initial brain CT scan, TAWF was very rare, and loss of MCA signals on TCD specifcally indicated the absence of blood flow in MCA.</P>

Performance Comparison of Two Gene Set Analysis Methods for Genome-wide Association Study Results: GSA-SNP vs i-GSEA4GWAS

Kwon, Ji-Sun,Kim, Ji-Hye,Nam, Doug-U,Kim, Sang-Soo Korea Genome Organization 2012 Genomics & informatics Vol.10 No.2

Gene set analysis (GSA) is useful in interpreting a genome-wide association study (GWAS) result in terms of biological mechanism. We compared the performance of two different GSA implementations that accept GWAS p-values of single nucleotide polymorphisms (SNPs) or gene-by-gene summaries thereof, GSA-SNP and i-GSEA4GWAS, under the same settings of inputs and parameters. GSA runs were made with two sets of p-values from a Korean type 2 diabetes mellitus GWAS study: 259,188 and 1,152,947 SNPs of the original and imputed genotype datasets, respectively. When Gene Ontology terms were used as gene sets, i-GSEA4GWAS produced 283 and 1,070 hits for the unimputed and imputed datasets, respectively. On the other hand, GSA-SNP reported 94 and 38 hits, respectively, for both datasets. Similar, but to a lesser degree, trends were observed with Kyoto Encyclopedia of Genes and Genomes (KEGG) gene sets as well. The huge number of hits by i-GSEA4GWAS for the imputed dataset was probably an artifact due to the scaling step in the algorithm. The decrease in hits by GSA-SNP for the imputed dataset may be due to the fact that it relies on Z-statistics, which is sensitive to variations in the background level of associations. Judicious evaluation of the GSA outcomes, perhaps based on multiple programs, is recommended.

Morphological characteristics of bud in chestnut hybrids

( U. K. Lee ),( Mahn Jo Kim ),( Moon Ho Lee ),( Yong Hee Kwon ),( Myong Soo Hwang ),( Sun Chang Kim ) 한국임학회 2005 한국임학회 학술발표논문집 Vol.2005 No.-

Risk of Hepatocellular Carcinoma in Patients with Chronic Hepatitis C Treated with Direct-Acting Antivirals

( Sun Hong Yoo ),( Jung Hyun Kwon ),( Hee Yeon Kim ),( Chang Wook Kim ),( Chan Ran You ),( Sang Wook Choi ),( Se Hyun Cho ),( Joon-Yeol Han ),( Do Seon Song ),( U Im Chang ),( Jin Mo Yang ),( Sung Won 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: The risk of hepatocellular carcinoma (HCC) in chronic hepatitis C (CHC) is decreased in patients achieved sustained viral response (SVR) after antiviral therapy based on pegylated interferon. However, the increasing risk of HCC development and recurrence in the patients who achieved SVR with all-oral DAA agents is controversy. The aim of this study was to evaluate the de-novo HCC development after antiviral therapy based on all-oral DAA agents in CHC patients without a history of previous HCC. Methods: A total of 963 CHC patients who were treated with interferon free regimen in the 8 affiliated hospitals of the Catholic University of Korea between March 2015 and March 2017 were retrospectively analyzed. De-novo HCC was defined as the newly development of HCC after DAA regimens but no prior HCC history before the DAA treatment. Results: Of the 963 patients, a total of 574 patients received DAA treatment who were followed up for at least 12 weeks with no history of previous HCC. The overall median follow-up period from the start of antiviral therapy was 10.4 months. SVR was achieved in 548 of the 574 (95.5%) patients. De-novo HCC was detected in 5 of 574 (0.87%) patients during the follow-up period. A univariate analysis showed that liver cirrhosis (p = 0.006) and a higher level of serum alpha fetoprotein (AFP) at the time of end treatment response (ETR) (p = 0.010) were significantly associated with HCC development. However, a multivariate analysis indicated a higher level of serum AFP at the time of ETR (p = 0.044, HR 11.80, 95% CI: 1.07-130.44) was the independent risk factor for HCC development. Conclusions: Our study demonstrates that there still exists a risk of newly HCC development in patients treated with all-oral-DAA agents for CHC. Also, serum AFP at the time of ETR was associated with HCC development and it may be a useful biomarker for the prediction of HCC development after all-oral-DAA agents for CHC.