기체치환포장을 이용한 팽이버섯의 저장성 연장에 관한 연구

박철,신완철,최석영 울산대학교 2005 생활과학논문집 Vol.7 No.1

저농도의 산소를 함유한 기체조성(G108)과 고농도의 이산화탄소를 함유한 기체조성(G604)을 이용하여 팽이버섯의 저장성 연장을 평가하였다. 팽이버섯을 실온(16℃)과 냉장저장(4℃)으로 나누어 저장하면서 중량감소률, pH 변화, 자루 신장률, PPO활성을 측정하였다. 실온저장 대조군은 14일 경과 후 중량이 16.3%까지 감소하였으나, 기체치환 저장군에서는 14일 경과 후 각각 3.5%, 2.0% 감소하였다. pH는 실온저장 대조군에서는 저장 3일째부터 감소하다가 7일째부터 증가하기 시작하여 저장 14일째에는 크게 상승하였다. 반면에 G108과 G604군에서는 pH변화가 거의 일어나지 않았다. 팽이버섯의 자루 신장률은 실온저장 3일에 37.6%, 10일에는 69.5%인 반면, G108과 G604군에서는 14일 기간 동안 신장률이 4.5%, 4.0% 였다. PPO 활성은 대조군에서는 3일째부터 지속적으로 증가하였으나 G108과 G604 모두 7일까지 증가가 일어나지 않았다. 냉장저장 시 대조군의 중량은 3일부터 감소하기 시작하여 28일에는 36.6%까지 감소하였으나, G108과 G604군에서는 28일까지 각각 2.1%, 2.0% 감소하였다. 대조군의 pH 변화는 저장 3일째부터 감소하다가 14일째부터 증가하기 시작하여 저장 28일째에는 크게 상승하였다. 반면에 G108과 G604군에서는 저장 28일까지 pH변화가 거의 일어나지 않았다. 냉장저장 시 자루 신장률은 28일 경과 후 대조군은 5.0%의 신장을 보였지만, G108군과 G604군에서는 각각 1.7%, 1.5% 신장하였다. PPO 활성은 대조근에서는 3일째부터 조금씩 증가하다가 21일부터는 크게 증가하였으나 G108과 G604 모두 저장 28일까지 거의 증가하지 않았다. 이상의 결과로 팽이버섯의 저장시 기체치환으로 버섯의 호흡을 억제함으로써 버섯의 저장성을 연장시킬 수 있음을 알 수 있었다. 실온저장 시 대조군은 3일부터 각종 품질변화가 일어나는 반면에, 기체치환저장은 14일까지 변화가 일어나지 않았으며, 냉장저장 시에는 대조군이 7일경부터 품질변화가 일어나는데, 지체치환저장은 28일까지 큰 변화가 없음을 알 수 있었다. The prolongation of shelf-life of winter mushroom(Flammulina velutipes) using low oxygen concentration(G108) and high carbon dioxide concentration(G604) was evaluated. The weight loss, pH change, stipe elongation and PPO activities were measured during 14 days at 16℃ or during 28 days at 4℃ storage. During 16℃ storage the weigh loss rate of control was 16.3% at 14 days, but that of G108 and G604 were 3.5% and 2.0% at 14 days respectively. The pH of control was decreased at 3 days, but increased after 7 days. However the pH of G108 and G604 were not changed during 14 days. The stipe elongation of control was 37.6% at 3 days and to 69.5% at 10 days, but the stipe of G108 and G604 were grown 4.5%, 4.0% during 14 days. Also, PPO activities of control were increased during 14 days, however that of G108 and G604 were not changed during 7 days. During 4℃ storage the weigh loss rate of control was 36.6% at 28 days, but that of G108 and G604 were little changed during 28 days. The pH of control was decreased at 3 days, but increased after 14 days. The pH of G108 and G604 were not changed during 28 days. The stipe elongation of control was 5.0% at 28 days, but the stipe of G108 and G604 were respectively 1.7% and 1.5% during 28 days. Also, PPO activities of control were continually increased during 28 days, that of G108 and G604 however were little changed during 28 days. These results suggested that the shelf-life of winter mushroom was prolonged by inhibiting the respiration of that using the modified atmosphere packaging. During 16℃ storage the qualities of control were not changed at 3 days, but that of G108 and G604 were not changed during 14 days. Also during 4℃ storage the qualities of control were not changes at 7 days, but that of G108 and G604 were not changed 28 days.

표고버섯의 저장성 연장에 관한 연구

박철,윤희진,신완철,최석영 울산대학교 2006 생활과학논문집 Vol.7 No.2

저농도의 산소를 함유한 기체조성(G108)과 고농도의 이산화탄소를 함유한 기체조성(G604)을 이용하여 표고버섯의 저장성 연장 효과를 평가하였다. 표고버섯을 실온(22°C)과 냉장저장(4°C)으로 나누어 저장하면서 중량감소율, pH 변화, 색도(L값), PPO 활성, 유리아미노산 함량을 측정하였다. 실온저장 대조군은 7일 경과 후 중량이 6.3%까지 감소하였으나, G108군과 G604군은 각각 3.0%, 3.2% 감소하였다. pH는 실온저장 대조군에서는 저장 3일째 급격히 감소하다가 7일에는 크게 상승하였다. 반면에 G108과 G604군에서는 pH 변화가 3일까지 변화가 없다가 7일에는 감소하였다. L값은 실온저장 3일에 대조군이 46.4인 반면, G108과 G6O4군은 7일에 각각 76.1, 76.6이었다. 냉장저장 시 대조군의 중량은 7일부터 감소하기 시작하여 35일에는 39.0%까지 감소하였으나, G108과 G6O4군에서는 42일까지 각각 3.1%, 3.0% 감소하였다. 대조군의 pH는 저장 21일까지 크게 감소하다가 증가하기 시작하여 저장 35일째에는 크게 상승하였다. 반면에 G108과 G604군에서는 저장 42일까지 pH변화가 거의 일어나지 않았다. 냉장저장 시 대조군의 L값은 14일 경과 후 큰 폭으로 떨어졌지만, G108군과 G6O4군은 42일까지 별로 감소하지 않았다. PPO 활성은 대조군에서는14일부터는 크게 증가하였으나 G108과 G604 모두 저장 42일까지 거의 증가하지 않았다또한 유리 아미노산 함량도 대조군에서는 크게 감소하였으나 기체치환군에서는 그 감소율이 크게 지연되었다. 이상의 결과로 표고버섯의 저장 시 기체치환으로 버섯의 호흡을 억제함으로써 버섯의 저장성을 연장시킬 수 있음을 알 수 있었다. 실온저장 시 대조군은 1일부터 각종 품질 변화가 일어나는 반면에, 기체치환저장은 3일까지 변화가 일어나지 않았으며, 냉장저장 시에는 대조군이 3일경부터 품질 변화가 일어나는데, 기체치환저장은 14일까지 품질의 큰 변화가 없음을 알 수 있었다. The prolongation of shelf-life of oak mushroom(Lentius edodes) using low oxygen concentration(G108) and high carbon dioxide concentration(G604) was evaluated. The weight loss, pH change, colour(L value), PPO activities and free amino acid contents were measured during 7 days at 22"C or during 42 days at 4'c storage. During 22"C storage the weight loss rate of control was 6.3% at 7 days, but that of G108 and G6O4 were 3.0% and3.2% at 7 days respectively. The pH of control was decreased at 3 days, but increased thereafter, However The pH of G108 and G6O4 were not changed during 3 days and were decreased thereafter. The colour (L value) of control was 46.4 at 3 days, but that of G108and G604 were 76.1, 76.6 during 7 days respectively. During 4"C storage the weigh loss rate of control was 39.0% at 35 days, but that of G108 and G6O4 were little changed during 42 days. The pH of control was decreased at 21 days, but increased after 35 days. The pH of G108 and G604 were not changed during 42 days. The colour (L value) of control was highly decreased at 14 days, but that of G108 and G6O4 were little changed during 42 days. Also, PPO activities of control were continually increased from 14 days, that of G108 and G6O4 however were little changed during 42 days. The contents of free amino acids of control were decreased continuously, but their decreasing rate was delayed in the groups of modified atmosphere packaging. These results suggested that the shelf-life of oak mushroom was prolonged by inhibiting the respiration of that using the modified atmosphere packaging. During 22'c storage the qualities of control were not changed at 1 days, but that of G108 and G6O4 were not changed during 3 days. Also during 4f storage the qualities of control were not changed at 3 days, but that of G108and G6O4 were not changed during 14 days.

Cyanidin and Cyanidin-3-O-β-D-glucoside Suppress the Inflammatory Responses of Obese Adipose Tissue by Inhibiting the Release of Chemokines MCP-1 and MRP-2

Suck-Young Choe,Mi-Ran Choe,Ji-Hye Kang,Hoon Yoo,양재하,Mi-Ok Kim,Rina Yu 한국식품영양과학회 2007 Preventive Nutrition and Food Science Vol.12 No.3

Obesity-induced inflammation plays a crucial role in obesity-related pathologies such as type II diabetes and atherosclerosis. Adipose tissue macrophages and the cell-derived proinflammatory chemokines are key components in augmenting inflammatory responses in obesity. Anthocyanins such as cyanidin and cyanidin-3-O-β-D-glucoside (C3G) are known to elicit anti-inflammatory activities by suppressing the production of proinflammatory mediators such as tumor necrosis factor alpha and nitric oxide in LPS-stimulated macrophages. In the present study, we investigated whether cyanidin and C3G have the potential to suppress the inflammatory responses of adipose cells. Cyanidin and C3G not only suppressed the migration of RAW 264.7 macrophages induced by mesenteric adipose tissue-conditioned medium, but also inhibited the activation of the cells to produce inflammatory chemokines such as monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-related protein-2 (MRP-2) in a dose-dependent manner. Cyanidin and C3G also inhibited the release of MCP-1 and MRP-2 from adipocytes and/or macrophages. These findings suggest that cyanidin and C3G may suppress the inflammatory responses of adipose tissue in obesity.

Effects of halogenated Dibenzo - p - Dioxins on Liver Excretory Function

Choe, Suck Young,Yang, Kyu Hwan,Yoo, Byung Sun 생화학분자생물학회 1987 BMB Reports Vol.14 No.2

Malonate-grown Pseudomonas fluorescence induced isocitrate lyase which is one of key enzymes of glyoxylate cycle. This finding indicated that carbon source for this bacteria might be acetyl-CoA generated by decarboxylation of malonyl-CoA. In consideration of the previous investigation that malonate-grown Pseudomonas also induced malonyl-CoA decarboxylase, the following metabolic pathway is suggested Malonate→Vlalonyl-CoA→Acetyl-CoA→Glyoxylate Cycle The malonate-induced isocitrate lyase was purified nearly 30 folds by the combination of ammonium sulfate precipitation, gel filtration and DEAE-cellulose ion exchange chromatography. Molecular size of this enzyme was estimated by gel filtration as 155,000.

Session B : Carbohydrates , Lipids and Natural Product Chemistry : EFFECTS OF BHA AND ACETAMINOPHEN ON THE BILIARY EXCRETION OF PHENOLPHTHALEIN AND THE HEPATIC GLUCURONIDATION IN MALE RATS

Suck Young Choe,Wha Jae Lim,Ri Na Yu 생화학분자생물학회(구 한국생화학분자생물학회) 1993 추계학술대회집 Vol.- No.-

Effects of BHA and BHT on Bromobenzene-induced Hepatotoxicity

Choe, Suck-Young 울산대학교 1987 연구논문집 Vol.18 No.1

식품에 항산화제로 첨가하는 BHA와 BHT의 bromobenzene에 의해 유발되는 간독성에 대한 영향을 조사 하였다. BHA와 BHT의 bromobenzene 을 처리하기 전 10일간 0.25%로 사료에 첨가하였다. BHA는 bromobenzene 에 의해 유발되는 BSP의 담즘을 통한 배설의 억제현상과 담즙배설 억제작용, 그리고 SGPT활성의 상승현상 모두에 대해 보호작용을 나타내었다. 그러나 BHT는 위의 모든 현상에 대해 보호작용을 나타내지 못하고 오히려 더욱 악화 시켰다. 또한 이들 항산화제는 in vitro 상태에서 malondialdehyde(MDA)의 증가에 대해 보호작용을 나타내지 못하였다. Effects of BHA and BHT on bromobenzene-induced hepatotoxicity were studied. BHA and BHT were given to rat in the diet at 0.25% level for 10 days prior to bromobenzene challenge. pretreatment with BHA retrieved the bromobenzene-incluced toxicity: i.e. recovered the reduced bilary excretion of BSP and the enhanced SGPT activity to control levels. However, BHT treatment resulted in a severe necrosis and further decreased the biliary excretion of BSP. Additon of bromobenzene to hepatocyte culture weakly induced the melondialdehyde(MDA) production and the addition of BHA and BHT did not prodect against the enhanced MDA production.

뇌동맥류에 대한 3차원 전산화단층촬영 혈관조영술의 진단적 가치

최일승,김영수,고용,오성훈,오석전,김남규,김광명,이승로 대한신경외과학회 1994 Journal of Korean neurosurgical society Vol.23 No.9

The authors have evaluated the efficacy of three dimensional computed tomographic angiography(3-DCTA) in diagnosing intracranial aneurysm. This literature studied thirty five patients harboring forty intracranial aneurysms using dynamic CT scan with intravenous contrast enhancement. After injection of 2㎎/㎏ of nonionic contrast material(Rayvist^(R)) intravenously, rapid dynamic CT scanning with thin section was performed. Three dimensional reconstruction was performed using the GE 9800 HR suface-rendering method with a threshold of about 100 Hounsfield units(HU). The authors comparatively analysed this 3-DCTA with conventional to surrounding vessels. While 3-DCTA clearly detected 38 aneurysms, conventional angiography did 37 ones. There was no significant difference between 3-DCTA and conventional angiography in demonstrating aneurysmal direction, but 3-DCTA was superior to conventional angiography in demonstrating aneurysmal shape, its neck and surrounding vascular structures. 3-DCTA showed deliberate anatomy of aneurysm and adjacent vessels through 3 dimensional image, and its operative view is helpful in surgical planning 3-DCTA might be expected to substitute for conventional angiography in the near future.

골반에서 발생한 혈관외피종의 뇌 전이 : 증례 보고

최일승,김영수,고용,오성훈,오석전,김남규,김광명 대한신경외과학회 1994 Journal of Korean neurosurgical society Vol.23 No.9

It is well known that intracranial hemangiopericytoma commonly metastasizes elsewhere in the body, espacially bone and lung. However, brain metastasis of extracranial hemangiopencytoma is very rare. The authors report a case of intracranial metastatic hemangiopericytoma from pelvis in a 41 year old woman. Neurologic symptoms developed 14 months after discovering the pelvic mass. After surgjcal excision, the clinical course was improved.

Cyanidin and Cyanidin-3-O-β-D-glucoside Suppress the Inflammatory Responses of Obese Adipose Tissue by Inhibiting the Release of Chemokines MCP-1 and MRP-2

Choe, Mi-Ran,Kang, Ji-Hye,Yoo, Hoon,Yang, Chae-Ha,Kim, Mi-Ok,Yu, Ri-Na,Choe, Suck-Young The Korean Society of Food Science and Nutrition 2007 Preventive Nutrition and Food Science Vol.12 No.3

Obesity-induced inflammation plays a crucial role in obesity-related pathologies such as type II diabetes and atherosclerosis. Adipose tissue macrophages and the cell-derived proinflammatory chemokines are key components in augmenting inflammatory responses in obesity. Anthocyanins such as cyanidin and $cyanidin-3-O-{\beta}-D-glucoside$ (C3G) are known to elicit anti-inflammatory activities by suppressing the production of proinflammatory mediators such as tumor necrosis factor alpha and nitric oxide in LPS-stimulated macrophages. In the present study, we investigated whether cyanidin and C3G have the potential to suppress the inflammatory responses of adipose cells. Cyanidin and C3G not only suppressed the migration of RAW 264.7 macrophages induced by mesenteric adipose tissue-conditioned medium, but also inhibited the activation of the cells to produce inflammatory chemokines such as monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-related protein-2 (MRP-2) in a dose-dependent manner. Cyanidin and C3G also inhibited the release of MCP-1 and MRP-2 from adipocytes and/or macrophages. These findings suggest that cyanidin and C3G may suppress the inflammatory responses of adipose tissue in obesity.

상·하악 전치부의 근관치료에 사용되는 시술길이 (Working Length)에 관한 통계학적 연구

최동훈,박동수,이찬영,이정식 大韓齒科保存學會 1984 Restorative Dentistry & Endodontics Vol.10 No.1

This study was undertaken to obtain the average canal length of upper & lower anterior teeth which was important in canal length measuring procedure of endodontic treatment. It was based upon 827 out-patients who had endodontic treatment on their upper & lower anterior teeth at the Department of Operative Dentistry, Dental Infirmary, Yonsei Medical Center from February, 1978 to June, 1984. The 1249 teeth of these patients were devided into sex and age groups. The root canal length of these teeth were measured. The following results were obtained; 1. The mean root canal length of upper & lower anterior teeth were as follows; Upper central incisors : 21.8mm Upper lateral incisors : 21.0mm Upper canines : 24.1mm Lower central incisors : 18.6mm Lower lateral incisors : 19.9mm Lower canines : 22.6mm 2. There was no significant difference in root canal length between sex. (p>0.05) 3. There was no significant difference in toot canal length between age groups. (p>0.05) 4. The distribution of upper central incisors showed the highest distribution followed by upper lateral incisors and lower central incisors between 10 to 40 year old age groups, and there was no significant difference in the rest of the age groups. There was no significant difference in sex distribution, which was 49.5% for males and 50.4% for females. The number of the upper anterior teeth was 74.3% of all the specimens and the lower anterior 25.7%, and 40.6% of all the specimens were upper central incisors.