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DNA Barcoding Study of the Family Braconidae (Hymenoptera)
Subin Choi,Hyojoong Kim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.10
Many species of braconids play an important role in controlling the populations of insects in agricultural and natural ecosystems, which parasitize pests. Nevertheless, the family Braconidae was scarcely research and DNA barcoding of Braconidae has not been studied in Korea. DNA barcoding can rapidly perform species identification, and classify difficult species in morphological identification. Because most braconids are very small and cryptic in morphology, DNA barcoding is useful in Braconidae taxonomy. In this study, genomic DNA of 280 individual samples were extracted, and, among them, 208 samples were obtained COI (cytochrome c oxidase I) barcode sequences. As a consequence of blast, 48 samples were identified in genus-level, and 51 sample were identified in species-level. Among them, 4 species were revealed as unrecorded in Korea.
Functional Characterization and Proteomic Analysis of Porcine Deltacoronavirus Accessory Protein NS7
( Subin Choi ),( Changhee Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.11
Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus that causes diarrhea in neonatal piglets. Like other coronaviruses, PDCoV encodes at least three accessory or species-specific proteins; however, the biological roles of these proteins in PDCoV replication remain undetermined. As a first step toward understanding the biology of the PDCoV accessory proteins, we established a stable porcine cell line constitutively expressing the PDCoV NS7 protein in order to investigate the functional characteristics of NS7 for viral replication. Confocal microscopy and subcellular fractionation revealed that the NS7 protein was extensively distributed in the mitochondria. Proteomic analysis was then conducted to assess the expression dynamics of the host proteins in the PDCoV NS7-expressing cells. Highresolution two-dimensional gel electrophoresis initially identified 48 protein spots which were differentially expressed in the presence of NS7. Seven of these spots, including two upregulated and five down-regulated protein spots, showed statistically significant alterations, and were selected for subsequent protein identification. The affected cellular proteins identified in this study were classified into functional groups involved in various cellular processes such as cytoskeleton networks and cell communication, metabolism, and protein biosynthesis. A substantial down-regulation of α-actinin-4 was confirmed in NS7-expressing and PDCoV-infected cells. These proteomic data will provide insights into the understanding of specific cellular responses to the accessory protein during PDCoV infection.
Two new records of the genus Aphidius (Braconidae: Aphidiinae) from South Korea
Subin Choi,Deok-Seo Ku,Hyojoong Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04
Aphidiinae in Braconidae includes more than 400 species within approximately 60 genera worldwide. Species of Aphidiinae are mostly known as solitary koinobiont endoparasitoids specifically associated with aphid hosts, and some of them are used for biological control. In the present study, two aphiniine species are newly recognized from South Korea based on morphological and molecular study: Aphidius rhopalosiphi de Stefani-Perez 1902 and A. uzbekistanicus Luzhetzki 1960. Although It was very difficult to discriminate two species morphologically, we cleary identified them by using COI (cytochrome c oxidase subunit I) DNA Barcode sequences. Here we present their morphological descriptions, illustrations and barcoding results.
α-Diazo oxime ethers for N-heterocycle synthesis
Choi, Subin,Ha, Sujin,Park, Cheol-Min The Royal Society of Chemistry 2017 Chemical communications Vol.53 No.45
<P>This Feature Article introduces the preparation and synthetic utility of alpha-diazo oxime ethers. alpha-Oximino carbenes are useful synthons for N-heterocycles, and can be easily prepared from alpha-diazo oxime ethers as precursors. We begin with the preparation of alpha-diazo oxime ethers and their application in [3+ 2] cycloaddition. It turns out that the nature of metals bound to carbenes plays a crucial role in modulating the reactivity of alpha-oximino carbenes, in which copper carbenes smoothly react with enamines, whereas the less reactive enol ethers and nitriles require gold carbenes. In Section 3.2, a discussion on N-O and C-H bond activation is presented. Carbenes derived from diazo oxime ethers show unique reactivity towards N-O and C-H bond activation, in which the proximity of the two functionalities, carbene and oxime ether, dictates the preferred reaction pathways toward pyridines, pyrroles, and 2H-azirines. In Section 3.3, the development of tandem reactions based on alpha-diazo oxime ethers is discussed. The nature of carbenes in which whether free carbenes or metal complexes are involved dissects the pathway and forms different types of 2H-azirines. The 2H-azirine formation turned out to be an excellent platform for the tandem synthesis of N-heterocycles including pyrroles and pyridines. In the last section, we describe the electrophilic activation of 2H-azirines with vinyl carbenes and oximino carbenes. The resulting azirinium species undergo rapid ring expansion rearrangements to form pyridines and pyrazines.</P>
A New Record of the Genus Toxares Haliday (Hymenoptera: Braconidae: Aphidiinae) from Korea
Subin Choi,Deok-Seo Ku,Hyojoong Kim 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
The genus Toxares (Braconidae: Aphidiinae) is reported for the first time in Korea. Members of Toxares are known as solitary koinobiont endoparasitoids especially specific for aphidine aphids. In this study, Toxares deltiger (Haliday, 1833) is found as new to Korea, which has a wide host range for 31 aphid species within Aphidinae. Thus, this species would be useful as natural enemy for biological control in the future. Diagnosis and host information are included.
Subin Choi,Il-Kwon Kim,Jongok Lim,Sunghoon Jung 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
The Genus Eugahania Mercet belongs to the family Encyrtidae (Hymenoptera: Chalcidoidea), containing 8 species in the world. They are mostly parasitoids on the nymphs of leafhoppers (Hemiptera). Until now, Eugahania have been barely studied in the world. In this study, the genus Eugahanua is reported for the first time from Korea including two new records. A key to the species, and diagnoses and photographs for each species are provided.