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Songjin Oh,Moongi Ji,Byeongchan Choi,Jonghye Yun,Jae-Hyun Park,In Duk Jung,Man-Jeong Paik 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11
Sepsis is a deleterious and systemic inflammatory response to infection. Despite advances in treatment, the mortality rate of sepsis remains high and its pathophysiology is not well understood. Thus, in this study, a metabolomic study was conducted for monitoring of metabolite changes and detection of biomarker for early diagnosis in mouse plasma samples of lipopolysaccharide-induced sepsis. Organic acid (OA) and fatty acid (FA) profiling analyses were performed by gas chromatography-tandem mass spectrometry as methoxime (MO)/tert-butyldimethylsilyl (TBDMS) derivatives. Amino acid (AA) profiling analysis was performed by liquid chromatography-tandem mass spectrometry. In this result, 14 OAs, nine FAs, and 34 AAs were determined. In the Mann-Whitney test, four OAs, three FFAs, and 17 AAs were significantly different (P < 0.05). In addition, star graphic pattern showed distorted shapes with characteristic and readily distinguishable patterns for each group. In the multivariate analysis of PCA and PLS-DA, two groups of control and sepsis showed clear separation. The major metabolites to distinguish between the two groups were identified as tyrosine, methionine, serine, proline, and asparagine. Therefore, the present metabolomic study will be useful for the biomarker detection in sepsis.
Byeongchan Choi,Moongi Ji,Songjin Oh,Mi ja Lee,Man-Jeong Paik 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11
Barley (Hordeum vulgare), a member of the grass family, is a major cereal grain grown in temperate climates globally. It is the most widely adapted cereal grain species with various nutrients and provided as a peeled form after milling in the market. This milling process is known to cause changes in the nutritional composition and taste of barley as well as metabolite levels. However, metabolomic study between pre- and post-milling barley was not fully understood. Among many metabolites, amino acids (AAs) are essential components of organisms, which play an important role for evaluation of various tastes. In addition, organic acids (OAs) contribute to the flavor of foods. Thus, in this study, metabolomic study of OA and AA was performed in three species (Saechalssalbori, Hinchalssalbori, and Yeongbaekchal) of pre- and post-milling barley. Profiling analyses of OAs as methoxime/tert-butyldimethylsilyl (TBDMS) derivatives and AAs as ethoxylcarbonyl-TBDMS derivatives were performed by gas chromatography-tandem mass spectrometry. The 36 metabolite levels of 16 OAs and 20 AAs were determined in the three species. Altered OAs and AAs were monitored in post-milling compared with pre-milling. In Saechalssalbori, α-ketoglutaric and pipecolic acid were significantly decreased (P-value < 0.05). In Hinchalssalbori, malonic acid, oxaloacetic acid, alanine, glycine, α-aminobutyric acid, leucine, isoleucine, proline, pipecolic acid, serine, γ-aminobutyric acid, phenylalanine, and glutamic acid were significantly decreased. In Yeongbaekchal, glycolic acid, 2-hydroxybutyric acid, fumaric acid, 4-hydroxyphenylacetic acid, 2-hydroxyglutaric acid, pipecolic acid, γ -aminobutyric acid, and tyrosine were significantly decreased, while α-ketoglutaric acid, cis-aconitic acid, α-aminobutyric acid, pyroglutamic acid, and glutamine were significantly increased. In PLS-DA score plot, the three species were completely separated between pre- and post-milling. Therefore, the present metabolomic study will be useful for the monitoring of altered metabolites following physical milling processes.