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Functional Characterization and Proteomic Analysis of Porcine Deltacoronavirus Accessory Protein NS7
( Subin Choi ),( Changhee Lee ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.11
Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus that causes diarrhea in neonatal piglets. Like other coronaviruses, PDCoV encodes at least three accessory or species-specific proteins; however, the biological roles of these proteins in PDCoV replication remain undetermined. As a first step toward understanding the biology of the PDCoV accessory proteins, we established a stable porcine cell line constitutively expressing the PDCoV NS7 protein in order to investigate the functional characteristics of NS7 for viral replication. Confocal microscopy and subcellular fractionation revealed that the NS7 protein was extensively distributed in the mitochondria. Proteomic analysis was then conducted to assess the expression dynamics of the host proteins in the PDCoV NS7-expressing cells. Highresolution two-dimensional gel electrophoresis initially identified 48 protein spots which were differentially expressed in the presence of NS7. Seven of these spots, including two upregulated and five down-regulated protein spots, showed statistically significant alterations, and were selected for subsequent protein identification. The affected cellular proteins identified in this study were classified into functional groups involved in various cellular processes such as cytoskeleton networks and cell communication, metabolism, and protein biosynthesis. A substantial down-regulation of α-actinin-4 was confirmed in NS7-expressing and PDCoV-infected cells. These proteomic data will provide insights into the understanding of specific cellular responses to the accessory protein during PDCoV infection.
DNA Barcoding Study of the Family Braconidae (Hymenoptera)
Subin Choi,Hyojoong Kim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.10
Many species of braconids play an important role in controlling the populations of insects in agricultural and natural ecosystems, which parasitize pests. Nevertheless, the family Braconidae was scarcely research and DNA barcoding of Braconidae has not been studied in Korea. DNA barcoding can rapidly perform species identification, and classify difficult species in morphological identification. Because most braconids are very small and cryptic in morphology, DNA barcoding is useful in Braconidae taxonomy. In this study, genomic DNA of 280 individual samples were extracted, and, among them, 208 samples were obtained COI (cytochrome c oxidase I) barcode sequences. As a consequence of blast, 48 samples were identified in genus-level, and 51 sample were identified in species-level. Among them, 4 species were revealed as unrecorded in Korea.
Choi Ji Young,Yun Jihye,Heo Subin,Kim Dong Wook,Choi Sang Hyun,Yoon Jiyoung,Kim Kyuwon,Jung Kee Wook,Myung Seung-Jae 대한영상의학회 2023 Korean Journal of Radiology Vol.24 No.11
Objective: Cine magnetic resonance imaging (MRI) has emerged as a noninvasive method to quantitatively assess bowel motility. However, its accuracy in measuring various degrees of small bowel motility has not been extensively evaluated. We aimed to draw a quantitative small bowel motility score from cine MRI and evaluate its performance in a population with varying degrees of small bowel motility. Materials and Methods: A total of 174 participants (28.5 ± 7.6 years; 135 males) underwent a 22-second-long cine MRI sequence (2-dimensional balanced turbo-field echo; 0.5 seconds per image) approximately 5 minutes after being intravenously administered 10 mg of scopolamine-N-butyl bromide to deliberately create diverse degrees of small bowel motility. In a manually segmented area of the small bowel, motility was automatically quantified using a nonrigid registration and calculated as a quantitative motility score. The mean value (MV) of motility grades visually assessed by two radiologists was used as a reference standard. The quantitative motility score’s correlation (Spearman’s ρ) with the reference standard and performance (area under the receiver operating characteristics curve [AUROC], sensitivity, and specificity) for diagnosing adynamic small bowel (MV of 1) were evaluated. Results: For the MV of the quantitative motility scores at grades 1, 1.5, 2, 2.5, and 3, the mean ± standard deviation values were 0.019 ± 0.003, 0.027 ± 0.010, 0.033 ± 0.008, 0.032 ± 0.009, and 0.043 ± 0.013, respectively. There was a significant positive correlation between the quantitative motility score and the MV (ρ = 0.531, P < 0.001). The AUROC value for diagnosing a MV of 1 (i.e., adynamic small bowel) was 0.953 (95% confidence interval, 0.923–0.984). Moreover, the optimal cutoff for the quantitative motility score was 0.024, with a sensitivity of 100% (15/15) and specificity of 89.9% (143/159). Conclusion: The quantitative motility score calculated from a cine MRI enables diagnosis of an adynamic small bowel, and potentially discerns various degrees of bowel motility.
Two new records of the genus Aphidius (Braconidae: Aphidiinae) from South Korea
Subin Choi,Deok-Seo Ku,Hyojoong Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04
Aphidiinae in Braconidae includes more than 400 species within approximately 60 genera worldwide. Species of Aphidiinae are mostly known as solitary koinobiont endoparasitoids specifically associated with aphid hosts, and some of them are used for biological control. In the present study, two aphiniine species are newly recognized from South Korea based on morphological and molecular study: Aphidius rhopalosiphi de Stefani-Perez 1902 and A. uzbekistanicus Luzhetzki 1960. Although It was very difficult to discriminate two species morphologically, we cleary identified them by using COI (cytochrome c oxidase subunit I) DNA Barcode sequences. Here we present their morphological descriptions, illustrations and barcoding results.
A First Record of Genus Testudobracon Quicke (Hymenoptera: Braconidae: Braconinae) from Korea
Subin Choi,Il-Kwon Kim,Hyojoong Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10
The genus Testudobracon (Braconidae: Braconinae) is reported for the first tine in Korea. The genus is a small genuswith 12 species distributed in the world, of which species are known as ectoparasitoids of the cecidomyiid (Diptera).Testudobracon species are distinguished from other braconid genus by the following characters: terminal flagellomere pointedbut without a prominent spine; mesoscutum largely setae, notauli well developed.In this study, a braconid genus, Testudobracon, is recorded from Korea for the firs time based on Testudobracon pleuralis(Ashmead, 1906). we provided diagnosis, host list and photographs of diagnostic characteristics for this species.
A New Record of Species of the Microplitis (Hymenoptera: Braconidae: Microgastrinae) in Korea
Choi, Subin,Kim, Hyojoong The Korean Society of Systematic Zoology 2018 Animal Systematics, Evolution and Diversity Vol.34 No.3
The genus Microplitis (Braconidae: Microgastrinae) is reported for the first time from Korea. The Microplitis has often quite small hypopygium, very short ovipositor and rugose propodeum with large aleola. The genus contains 190 described species, among them 19 speceis were recorded in Korea. Microplitis varicolor was established by Viereck in 1917. The species is found as new to Korea in this study. Microplitis varicolor is endoparasitoid, especially attacking species of Noctuidae (Insecta: Lepidoptera) including pests of leguminous crop. In this study, diagnosis, photographs and host lists are included.