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RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단
배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2
Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.
Application of temperature-controlled metabolic chamber in mice
Su In Jang,Seul Gi Yoon,Hye Sun Go,Yong Jae Kim,Hak Su Kim,Ji Hyeon Baek,Tae Ho Kim,Ji Min Choi,Soo Kyung Kang,Da In On,Hyun A Noh,Il Yong Kim,Je Kyung Seong 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Recently, research on various metabolic diseases such as obesity and diabetes has been in the spotlight. A representative example of this is the study of metabolic function analysis using Metabolic cage. It is believed that these studies will greatly contribute to the prevention and treatment of various metabolic diseases in humans. This study focuses on the study of obesity in mice. Mouse metabolic phenotyping has been widely used for detecting metabolic disorder in genetically engineered mouse. Korea Mouse Phenotyping Center (KMPC), nation-wide program for mouse production and phenotyping in Korea has been establishing several pipelines for disease-specific mouse phenotyping to support the mouse research. KMPC has been providing mouse metabolic phenotyping including high fat diet, exercise and cold challenges. Multiple parameter including energy expenditure (EE), O2/CO2 consumption (RER), heat generation and activity has been provided with histology service and body composition. Also temperature can be measured during metabolic chamber with telemetric system. Brown adipogenesis is one of key mechanism for developing new candidate drug target for curing obesity and type 2 diabetes. Several factors including cold, adrenergic b3 agonist and exercise are well known factor leading to brown adipogenesis. Here we introduce mouse metabolic phenotyping services with environmental control chamber at KMPC. Temperature-controlled metabolic cage, the climate chambers can control temperatures from 4 °C to 35 °C, while measuring energy consumption using oxygen consumption, carbon dioxide production, and respiratory exchange ratio and food intake. Using this, exposing the mouse to a low temperature environment can activate BAT by browning WAT, which causes various diseases such as heart disease, diabetes, and obesity. The changes of metabolic parameter was finely measured during cold exposure with temperature-controlled metabolic cage.
( Su Jong Yu ),( Jung-hwan Yoon ),( Won Kim ),( Jeong Min Lee ),( Yun Bin Lee ),( Yuri Cho ),( Dong Hyeon Lee ),( Minjong Lee ),( Jeong-ju Yoo ),( Eun Ju Cho ),( Jeong-hoon Lee ),( Yoon Jun Kim ),( Ch 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.2
Background/Aims: Liver transplantation offers the only definite cure for cirrhosis but lacking donors is problem. Stem cell therapy is attractive in this setting. In this study, we aimed to explore the safety and efficacy of ultrasound-guided percutaneous portal transplantation of peripheral blood monocyte cell (PBMC) in cirrhotic patients. Methods: A total of nine decompensated cirrhotic patients were randomized into three groups: group 1 (n = 3) was control group, group 2 (n = 3) received granulocyte-colony stimulating factor (G-CSF) mobilization for 3 days, and group 3 (n = 3) received G-CSF mobilized PBMCs by leukapheresis and PBMC transplantation through ultrasound-guided percutaneous portal vein puncture. Liver function and clinical features were evaluated. Results: At baseline, the Child-Turcotte-Pugh and the model for end-stage liver disease scores were comparable in study groups. Compared with group 1, there was a tendency to improve liver function in group 3 at 6 months after treatment. Treatment was tolerable and no complications were encountered related to the G-CSF mobilization or percutaneous portal administration of PBMCs. Imaging studies showed patent portal veins at the end of the study period. Conclusions: Autologous PBMC transplantation through ultrasound-guided percutaneous portal vein puncture could be considered as a safe alternative treatment for decompensated cirrhotic patients.
Effect of Formal Education Level on Measurement of Rheumatoid Arthritis Disease Activity
( Hyeon Su Kim ),( Ui Hong Jung ),( Hyesun Lee ),( Seong Kyu Kim ),( Hwajeong Lee ),( Jung Yoon Choe ),( Sang Gyu Kwak ),( Theodore Pincus ),( Sung Hoon Park ) 대한류마티스학회 2015 대한류마티스학회지 Vol.22 No.4
Objective. The aim of this study is to analyze the capacity of three demographic variables - age, sex, and formal education level - as well as disease duration to explain variation in 7 Core Data Set variables and 4 indices used to assess rheumatoid arthritis (RA), in a cohort of Korean patients seen in usual care. Methods. All RA Core Data Set measures were collected in usual care of 397 RA patients, including tender/swollen joint counts (TJC, SJC) 28, physician global estimate of status, erythrocyte sedimentation rate, C-reactive protein, and a multidimensional health assessment questionnaire to assess physical function, pain, and patient global estimate of status (PATGL). Four indices were computed: disease activity score with 28 joint count (DAS28), simplified disease activity index (SDAI), clinical disease activity index (CDAI), and routine assessment of patient index data 3 (RAPID3). Descriptive statistics and multivariate generalized linear models were used in data analysis. Results. Patients with lower education had higher scores, indicating greater severity, for all 7 Core Data Set measures and 4 indices (significant for TJC, function, pain, PATGL, DAS28, SDAI, CDAI, RAPID3). In a series of regressions that included age, sex, disease duration, and education, formal education level was the only significant variable to explain variation in TJC, pain, PATGL, physician global estimate of status (DOCGL), DAS28, SDAI, CDAI, and RAPID3. Conclusion. Significant associations with education were found in Korean RA patients according to most RA Core Data Set measures and 4 indices. Education was more likely than age, sex, or disease duration to explain variation in most measures and indices. (J Rheum Dis 2015;22:231-237)
( Su Jong Yu ),( Hyunsoo Kim ),( Hophil Min ),( Areum Sohn ),( Young Youn Cho ),( Jeong-ju Yoo ),( Dong Hyeon Lee ),( Eun Ju Cho ),( Jeong-hoon Lee ),( Jungsoo Gim ),( Taesung Park ),( Yoon Jun Kim ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Achievement of a complete response (CR) after transarterial chemoembolization (TACE) is the most robust predictor of favorable outcomes in patients with hepatocellular carcinoma (HCC). The aim of this study was to identify blood-based biomarkers to predict a sustained CR after TACE using targeted proteomics. Methods: Consecutive patients with HCC who had undergone TACE were drawn from our prospective cohort [training set (n=100) and validation set (n=80)]. Serum samples were obtained before and 6 months after TACE. Treatment responses were evaluated using the modified Response Evaluation Criteria in Solid Tumors (mRECIST). Multiple reaction monitoring-mass spectrometry (MRM-MS) was used to measure marker candidate proteins (MCPs) with regard to their association with the recurrence of HCC and a sustained CR after TACE. Results: In the training set, the MRM-MS assay identified 5 MCPs (MRM-MS marker panel). When this 5-marker panel was combined with the best-performing clinical variables (tumor number, baseline PIVKA, and baseline AFP), the resulting ensemble model had the highest area under the receiver operating curve (AUROC) value in predicting a sustained CR after TACE in the training and validation sets (0.881 and 0.813, respectively). Further, the ensemble model remained an independent predictor of rapid progression (hazard ratio, 2.889; 95% confidence interval, 1.612-5.178; P<0.001) in the entire population by multivariate analysis. Conclusions: Our ensemble model before TACE can predict a sustained CR after TACE. Therefore, this model can aid in determining the best candidates for TACE and the need for adjuvant therapy.
Effect of pelvic artery embolization on AMH level in postpartum hemorrhage patients
( Su Min Hyun ),( Eun Ji Oh ),( Kyong-no Lee ),( Hyeon Ji Kim ),( Jee Yoon Park ),( Kyung Joon Oh ) 대한산부인과학회 2022 대한산부인과학회 학술대회 Vol.108 No.-
Objective: In postpartum hemorrhage (PPH) patients, pelvic artery embolization (PAE) is one of treatment options that can avoid hysterectomy. However, the impact of PAE on ovarian function in women wishing to retain their fertility remains controversial. Therefore, we tried to find out whether PAE affects the ovarian reservation by measuring in AMH (anti-mllerian hormone) level. Methods: This study is retrospective analysis of prospectively collected sample of AMH at the time of average 12 weeks after delivery (median 87 days) from August 2020 to April 2022. A total number of patients who underwent embolization due to PPH was 31, consisting of 29 patients referred from other hospital and 4 patients delivered at our hospital. Each patient underwent PAE for one of uterine, ovarian, internal iliac, vaginal, or rectal artery using gelfoam or glue. Inverse probability of treatment weighing method was used to minimize bias. The variables were adjusted using maternal age, multifetal pregnancy, mode of delivery, parity, and underlying disease as matching variables. Multivariable analysis was done by adjusting interval from delivery to blood sampling, body mass index, breast feeding, menstruation, and underlying disease. Results: In 31 patients who underwent PAE, the materials used for embolization were gelfoam for 23 patients, glue for 3 patients, and both gelfoam and glue for 5 patients. Target vessels for embolization were as follows; 29 cases of uterine artery, 1 case of ovarian artery, 2 cases of internal iliac artery, 2 cases of vaginal artery, and 1 case of rectal artery. Multivariable analysis showed that there is no difference in AMH levels according to whether PAE is done or not. (estimates -0.52, confidence interval -1.62~0.58, p value=0.35) Conclusion: PAE does not seem to affect ovarian reserve, as measured by AMH level.