Calcium overload is essential for the acceleration of staurosporine-induced cell death following neuronal differentiation in PC12 cells

Su Ryeon Seo,서정택 생화학분자생물학회 2009 Experimental and molecular medicine Vol.41 No.4

Differentiation of neuronal cells has been shown to accelerate stress-induced cell death, but the underlying mechanisms are not completely understood. Here, we find that early and sustained increase in cytosolic ([Ca2+]c) and mitochondrial Ca2+ levels ([Ca2+]m) is essential for the increased sensitivity to staurosporineinduced cell death following neuronal differentiation in PC12 cells. Consistently, pretreatment of differentiated PC12 cells with the intracellular Ca2+-chelator EGTA-AM diminished staurosporine-induced PARP cleavage and cell death. Furthermore, Ca2+ overload and enhanced vulnerability to staurosporine in differentiated cells were prevented by Bcl-XL overexpression. Our data reveal a new regulatory role for differentiation- dependent alteration of Ca2+ signaling in cell death in response to staurosporine.

Studies on Anti-inflammatory and Anti-aging activity of Fermented Ligustrum Fructus

Su Ryeon CHOI,Eun Jin SHIN,Kee-Young LEE,Sang Moon KANG,Mi Kyeong LEE,Hyung Seo HWANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

The dried fruit of Ligustrum lucidum, Ligustici Fructus, is known to have anti-cancer and anti-inflammatory properties as well as skin improvement. Phytochemical studies of L. lucidum fruit have resulted in the isolation of novel active compounds and the identification of their roles in antioxidant and anti-inflammatory activities. To obtain a large amount of active ingredients, Ligustici Fructus extract was fermented under various conditions using three types of microorganisms: Galactomyces Reesii, Bacillus methylotrophicus, and Saccharomyces cerevisiae. A total of 16 fermented extracts were obtained and effective fermented extracts were screened through DPPH/ABTS radical scavenging analysis and nitric oxide analysis. Among them, E-BF-WB (80% EtOH extract of Ligustici Fructus fermented without broth by Bacillus methylotrophicus) showed the highest activity. First, E-BF-WB significantly inhibited the expression of iNOS, COX-2 gene and inflammatory cytokines IL-1α/β/-6 and TNF-α in LPS-induced RAW264.7 marcophage cells. In addition, MMP-1/2/3/9 involved in wrinkling was significantly reduced in TNF-α-induced Hs68 fibroblasts by E-BF-WB. From these results, we confirmed that E-BF-WB has antioxidant, anti-inflammatory and skin wrinkle improvement functions. These results suggest that E-BF-WB will be developed as a new bio-cosmetic material for skin soothing and anti-aging cosmetics in the future.

TPMS 구조를 적용한 미세먼지 필터 성능 최적화

황수련(Su Ryeon Hwang),김지연(Ji Yeon Kim),김민서(Min Seo Kim),김희라(Hee Ra Kim),정성용(Sung Yong Jung) 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.6

면역세포에서 Bioconversion 전후 제주 감귤 과피 추출물의 항염증 효과

서지은(Jieun Seo),임희진(Heejin Lim),장윤희(Yun-Hee Chang),박혜련(Hye-Ryeon Park),한복경(Bok-Kyung Han),정중기(Jung-Ky Jeong),최경숙(Kyoung-Sook Choi),박수범(Su-Beom Park),최혁준(Hyuk-Joon Choi),황진아(Jinah Hwang) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.3

감귤류와 감귤류의 과피는 오랜 기간 동양의학의 약재로 사용되어 왔고 최근에는 생과와 주스로 많이 소비되고 있다. 하지만 감귤류 가공 공정 시 많은 과피 부산물이 발생하므로 활용방안이 필요하다. 감귤류의 과피에는 플라바논(flavanone)이 풍부하며 이 플라바논의 형태 중 배당체보다 비배당체의 체내 흡수가 더 효과적이므로 비배당체가 생리적 효과가 더 뛰어나다. Bioconversion(물질전환)은 cytolase에 의해 narirutin과 naringin은 naringenin으로, hesperidin은 hesperetin으로 각각 전환되며, 본 연구는 감귤의 과피에 다량 존재하는 플라바논을 물질전환 한 뒤 면역세포에서 항산화 및 항염증 효과를 확인하였다. 물질전환 후에 감귤 과피 추출물의 전자공여능이 농도 의존적으로 높아지는 것을 확인하였고, 면역세포인 RAW264.7에 200, 500 μg/mL 감귤 bioconversion 전(CU)?후(CU-C) 과피 추출물을 4시간 동안 처리한 후 LPS(1 μg/mL, 8시간)를 처리하였다. 염증 관련 효소인 iNOS와 COX-2의 mRNA와 단백질 발현을 확인한 결과 물질전환과 관계없이 감귤 과피 추출물이 LPS에 의해 유도된 iNOS와 COX-2의 mRNA와 단백질 발현을 농도 의존적으로 감소시켰지만 물질전환 전보다 후 추출물의 억제 효과가 더 높았다. iNOS에 의해 생성되는 NO 역시 감귤 과피 추출물이 LPS에 의해 유도된 NO 생성을 억제시켰다. 본 연구 결과는 감귤 과피 추출물이 항산화와 항염증 생리활성을 보였지만 cytolase를 이용하여 물질전환 한 감귤 과피 추출물이 물질전환 전보다 이러한 생리활성이 강화됨을 보임으로써 향후 감귤류 부산물로 폐기되어온 감귤류 과피가 산화적 스트레스와 염증반응에 기인하는 만성질환을 위한 기능성 소재로 활용이 가능할 것으로 기대된다. 감귤 과피 추출물의 in vitro 상에서 검증된 항염증 효능이 향후에 in vivo 상에서 여러 염증 조직에 미치는 영향에 대한 추가 검증이 필요하다고 사료된다. Citrus and its peels, which are by-products from juice and/or jam processing, have long been used in Asian folk medicine. Citrus peels show an abundant variety of flavanones, and these flavanones have glycone and aglycone forms. Aglycones are more potent than glycones with a variety of physiological functions since aglycone absorption is more efficient than glycones. Bioconversion with cytolase converted narirutin and naringin into naringenin and hesperidin into hesperetin. Therefore, this study aimed to investigate the anti-oxidant and anti-inflammatory effects of bioconversion of Citrus unshiu (CU) peel extracts with cytolase (CU-C) in RAW264.7 cells. HPLC chromatograms showed that CU and CU-C had 23.42% and 29.39% total flavonoids, respectively. There was substantial bioconversion of narirutin to naringenin and of hesperidin to hesperetin. All citrus peel extracts showed DPPH scavenging activities in a dose-dependent manner, and CU-C was more potent than intact CU. RAW264.7 cells were pre-treated with 0∼500 μg/mL of citrus peel extracts for 4 h and then stimulated by 1 μg/mL of lipopolysaccharide (LPS) for 8 h. All citrus peel extracts showed decreased mRNA levels and protein expression of LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner. Especially, CU-C markedly inhibited mRNA and protein expression of iNOS and COX-2 compared to intact citrus peel extracts. All citrus peel extracts showed decreased NO production by iNOS activity. This result suggests that bioconversion of citrus peel extracts with cytolase may provide potent functional food materials for prevention of chronic diseases attributable to oxidation and inflammation by boosting the anti-inflammatory effects of citrus peels.

하드디스크의 잔존 수명 예측에 1D CNN-LSTM 을 이용한 모델 적용 연구

서양진 ( Yangjin Seo ) 한국정보처리학회 2020 한국정보처리학회 학술대회논문집 Vol.27 No.2

제품이나 부품의 잔존 수명을 정확하게 예측할 수 있다면 고장이나 중단으로 인한 손실을 방지하는 것이 가능해질 것이다. 제품의 잔존 수명은 시계열 데이터 분석을 통해 예측될 수 있으며, 최근에는 딥러닝을 이용한 잔존 수명 예측 연구가 활발하게 진행되고 있다. 본 연구에서 우리는 컴퓨터 기반 시스템의 주요 고장 요소가 되고 있는 하드디스크의 잔존 수명을 예측하는 문제에 1D CNN-LSTM 을 이용한 모델을 적용하고, RMSE 와 R-Square 값을 이용해 적용한 모델의 성능을 평가하였다.

NF-kappaB-inducing kinase phosphorylates and blocks the degradation of Down syndrome candidate region 1.

Lee, Eun Jung,Seo, Su Ryeon,Um, Ji Won,Park, Joongkyu,Oh, Yohan,Chung, Kwang Chul American Society for Biochemistry and Molecular Bi 2008 The Journal of biological chemistry Vol.283 No.6

<P>Down syndrome, the most frequent genetic disorder, is characterized by an extra copy of all or part of chromosome 21. Down syndrome candidate region 1 (DSCR1) gene, which is located on chromosome 21, is highly expressed in the brain of Down syndrome patients. Although its cellular function remains unknown, DSCR1 expression is linked to inflammation, angiogenesis, and cardiac development. To explore the functional role of DSCR1 and the regulation of its expression, we searched for novel DSCR1-interacting proteins using a yeast two-hybrid assay. Using a human fetal brain library, we found that DSCR1 interacts with NF-kappaB-inducing kinase (NIK). Furthermore, we demonstrate that NIK specifically interacts with and phosphorylates the C-terminal region of DSCR1 in immortalized hippocampal cells as well as in primary cortical neurons. This NIK-mediated phosphorylation of DSCR1 increases its protein stability and blocks its proteasomal degradation, the effects of which lead to an increase in soluble and insoluble DSCR1 levels. We show that an increase in insoluble DSCR1 levels results in the formation of cytosolic aggregates. Interestingly, we found that whereas the formation of these inclusions does not significantly alter the viability of neuronal cells, the overexpression of DSCR1 without the formation of aggregates is cytotoxic.</P>

The acute effects of hydrocortisone on cardiac electrocardiography, action potentials, intracellular calcium, and contraction: The role of protein kinase C

Park, Mi-Hyeong,Park, Seo-In,Kim, Jong-Hui,Yu, Jing,Lee, Eun Hye,Seo, Su Ryeon,Jo, Su-Hyun North-Holland 2019 Molecular and cellular endocrinology Vol.494 No.-

<P><B>Abstract</B></P> <P>Hydrocortisone exerts adverse effects on various organs, including the heart. This study investigated the still unclear effects of hydrocortisone on electrophysiological and biochemical aspects of cardiac excitation–contraction coupling. In guinea pigs’ hearts, hydrocortisone administration reduced the QT interval of ECG and the action potential duration (APD). In guinea pig ventricular myocytes, hydrocortisone reduced contraction and Ca<SUP>2+</SUP> transient amplitudes. These reductions and the effects on APD were prevented by pretreatment with the protein kinase C (PKC) inhibitor staurosporine. In an overexpression system of <I>Xenopus</I> oocytes, hydrocortisone increased hERG K<SUP>+</SUP> currents and reduced Kv1.5 K<SUP>+</SUP> currents; these effects were negated by pretreatment with staurosporine. Western blot analysis revealed dose- and time-dependent changes in PKCα/βII, PKCε, and PKCγ phosphorylation by hydrocortisone in guinea pig ventricular myocytes. Therefore, hydrocortisone can acutely affect cardiac excitation–contraction coupling, including ion channel activity, APD, ECG, Ca<SUP>2+</SUP> transients, and contraction, possibly via biochemical changes in PKC.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Hydrocortisone decreased QT interval and action potential duration in cardiomyocytes. </LI> <LI> Hydrocortisone reduced amplitude of contraction and Ca<SUP>2+</SUP> transient in cardiomyocytes. </LI> <LI> Hydrocortisone increased hERG K<SUP>+</SUP> current and decreased Kv1.5 K<SUP>+</SUP> current. </LI> <LI> PKC inhibitor prevented the effects of hydrocortisone on cardiac electrophysiology. </LI> <LI> Hydrocortisone activated PKCα/βII and PKCε in cardiomyocytes. </LI> </UL> </P>

Vascular Biology : TIMP-2: An Endogenous Inhibitor of Angiogenesis

서동완 ( Dong Wan Seo ),( Soo Hyeon Kim ),( Su Ryeon Seo ),( Gie Taek Chun ),( Nam Soo Kim ),( Pyeung Hyeun Kim ),( Se Won Yie ),( Woo Hyeon Byeon ) 한국지질동맥경화학회(구 한국지질학회) 2007 한국지질동맥경화학회 추계학술발표논문집 Vol.2007 No.-

Invited Mini Review : Neuroprotective roles of pituitary adenylate cyclase-activating polypeptide in neurodegenerative diseases

( Eun Hye Lee ),( Su Ryeon Seo ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.7

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic bioactive peptide that was first isolated from an ovine hypothalamus in 1989. PACAP belongs to the secretin/glucagon/vasoactive intestinal polypeptide (VIP) superfamily. PACAP is widely distributed in the central and peripheral nervous systems and acts as a neurotransmitter, neuromodulator, and neurotrophic factor via three major receptors (PAC1, VPAC1, and VPAC2). Recent studies have shown a neuroprotective role of PACAP using in vitro and in vivo models. In this review, we briefly summarize the current findings on the neurotrophic and neuroprotective effects of PACAP in different brain injury models, such as cerebral ischemia, Parkinson`s disease (PD), and Alzheimer`s disease (AD). This review will provide information for the future development of therapeutic strategies in treatment of these neurodegenerative diseases. [BMB Reports 2014; 47(7): 369-375]

Propionibacterium acnes에 의한 염증반응에서 Eurya persicifolia Gagnep. 추출물의 억제효과

신진학,서수련,Shin, Jin Hak,Seo, Su Ryeon 한국미생물학회 2019 미생물학회지 Vol.55 No.3

여드름은 일반적인 피부 염증성 질환으로 알려져 있다. 여드름은 모낭 내 피지선에서 나타나는 만성 염증 질환이다. Propionibacterium acnes (P. acnes)의 증식은 대식세포가 염증성 사이토카인을 분비하도록 자극한다. 최근 연구에서 여러 천연 추출물이 P. acnes에 의해 매개되는 염증반응을 감소시키는 것을 확인하였다. 그러나 P. acnes에 의한 염증반응에서 E. persicifolia Gagnep. (E. persicifolia) 추출물의 억제효과에 관한 연구는 수행되지 않았다. 따라서 본 연구에서는 P. acnes에 의해 유도된 염증 반응에서 E. persicifolia 추출물의 항 염증효과를 조사하였다. P. persicifolia 추출물은 마우스 대식세포주인 RAW 264.7에서 P. acnes에 의해 유도된 IL-$1{\beta}$, IL-6, TNF-${\alpha}$ 및 iNOS와 같은 염증 매개체의 발현 수준을 억제하였다. 또한 E. persicifolia 추출물이 염증성 사이토카인 발현의 주요 조절인자인 NF-${\kappa}B$ 전사 활성화를 억제한다는 것을 발견했다. 본 연구 결과는 P. acnes의 치료를 위한 잠재적인 치료물질로서 E. persicifolia 추출물을 제안한다. Acne is a chronic inflammatory disease outbreak in the sebaceous glands within the hair follicle. The proliferation of Propionibacterium acnes (P. acnes) causes monocytes to stimulate secretion of inflammatory cytokines. A number of studies proposed the inhibitory effects of P. acnes-mediated inflammation by several natural extracts. However, studies on the effect of Eurya persicifolia Gagnep. (E. persicifolia) extracts on the inflammatory responses by P. acnes have not been explored yet. In this study, we investigated the anti-inflammatory effect of E. persicifolia extract in the inflammatory reactions induced by P. acnes. We found that E. persicifolia extract successfully diminished the expression levels of inflammatory mediators such as IL-$1{\beta}$, IL-6, TNF-${\alpha}$, and iNOS in P. acnes-activated mouse macrophage RAW 264.7 cells. We found that the immunosuppressive effect of E. persicifolia extract in the P. acnes-activated inflammatory signaling is mediated by the regulation of NF-${\kappa}B$ transcriptional activation, which is a key regulator of inflammatory cytokine expression. Our results suggest that E. persicifolia extract held potentials for the treatment of P. acnes by suppressing NF-${\kappa}B$ signaling pathways.