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李敏載,河永七,李光雄,邊宇玄 서울대학교 1975 서울대학교 論文集 Vol.25 No.-
Serveral physiological characterstics of sulfuroxidizing bacteria such as Thiobacillus thiooxidans and T. concretivorus are described. 1) Effect of pH on inhibitory function of pyruvate. The rate of oxygen uptake was reduced in accordance with decrease of pH. And on the other hand inhibition rate of pyruvate was more severe in lower pH than in higher pH. High concentration of hydrogen ion seemed to accelerate inhibitory function of pyruvate. Limiting concentration of pyruvate which absolutely inhibit oxygen uptake was lowered with time. In O time, 10^-2M of pyruvate absolutely inhibited oxygen uptake in pH 5. After 24 hours limiting concentration was lowered to 10^-3M. 2) Effect of organic compounds on the oxygen uptake of T. concretivorns. Effect of glucose, fructose, xylose, glutamate, succinate, malate, glycine, lactate, acetate, pyruvate, citrate, formate, and cisaconitate on thiosulfate oxidation and availability of these compounds as sloe source of energy by T. concretivorus were observed. A 0.5% concentration of malate and glycine accelerated thiosulfate oxidation almost react somewhat inhibitory. Pyruvate and citrate inhibited thiosulfate oxidation. In thiosulfatefree medium, organic compounds except formate and pyruvate affected no significant influences on oxygen uptake. 3) Enzyme assay. Of enzymes concerned TCA cycle and glycolysis, T. concretivorus had most of those enzyme activities even though they were low. Activities of hexokinase and succinic dehydrogenase were somewhat high and that of aconitase was very low on the contrary. 4) Optimum condition of mass culture of sulfur oxidizing bacteria. In a large jar fermenter opimum conditions including agitations speed, volume of supplying gases, and constitution rate of oxygen and carbon dioxide, etc. were experimented. The sulfuroxidier, T. thiooxidans was grown most effectively under the condition of automatically controlled pH of 1.0, and with the aeration of oxygen and carbon dioxide mixture (5 : 1) at the flow rate of 0.05 vvm, and at the agitation velocity of 200 rpm in this laboratory scale fermenter.
Development of a Short Form Depression Screening Questionnaire for Korean Soldiers
Byeon Gihwan,Jo Sun-Jin,Lee Hae-Woo,Yim Hyeon-Woo,Park Jong-Ik 대한의학회 2021 Journal of Korean medical science Vol.36 No.27
Background: The frequencies of South Korean soldiers' depression and resulting suicide are increasing every year. Thus, this study aimed to develop and confirm the reliability and validity of a simple short form depression screening scale for soldiers. Methods: This study was conducted as part of a 2013 research project named ‘The Epidemiological Study on the Prevalence of Depression in Military Service and a Search for High Risk Group Management.’ Clinical depression was diagnosed using the Korean version of the Mini International Neuropsychiatric Interview and suicide risk was assessed through the Korean version of the Composite International Diagnostic Interview. Furthermore, the Center for Epidemiological Studies for Depression Scale (CES-D), the Stress Response Inventory, and the Barret Impulsiveness Scale were employed. Of the 20 CES-D items, three of the most correlated items with clinical diagnosis were derived to form the short form scale. Analyses for internal consistency, concurrent validity, and factor analysis were implemented for its validation. We performed a receiver operating curve (ROC) analysis using a clinical diagnosis of depression as a gold standard to calculate the area under the curve (AUC) value, cut-off score, and corresponding sensitivity and specificity to that cut-off score. Results: According to the results of the correlation analysis, 7, 18, and 4 were selected to be on our scale. The three-item scale was reliable with a Cronbach's alpha value of 0.720, and a factor was derived from the factor analysis. The ROC analysis showed a high discriminant validity, with an AUC value of 0.891. The sensitivity and specificity were 84.8% and 78.2%, and 71.7% and 91.6%, respectively, for each when the selected cut-off scores were 2 and 3, respectively. Depression screened through the scale when the cut-off score was 2 or 3 was significantly associated with suicidality, stress, and social support. Conclusion: The depression screening questionnaire for Korean soldiers developed through this study demonstrated high reliability and validity. Since it comprises only three items, it can be utilized easily and frequently. It is expected to be employed in a large-scale suicide prevention project targeting military soldiers in the future; it will be beneficial in selecting high-risk groups for depression.
Seroprevalence of tuberculosis in domesticated elk (<i>Cervus canadensis</i>) in Korea
Kang, Shin-Seok,Byeon, Hyeon-Seop,Ku, Bok Kyung,Kim, Sang Woo,Kim, Jiro,Woo, Jeongim,Ahn, Byeongwoo,Kim, Soojin,Monoldorova, Sezim,Park, Chan-Ho,Cho, Sang-Nae,Jeon, Bo-Young Elsevier 2016 Research in veterinary science Vol.107 No.-
<P><B>Abstract</B></P> <P>Tuberculosis is a contagious disease in animals, primarily cattle, although it also affects wild animals and humans. There are few data on the state of tuberculosis in domesticated elk (<I>Cervus canadensis</I>) in Korea. In order to investigate tuberculosis in elk, the effectiveness of an enzyme linked immunosorbent assay (ELISA) using MPB70 and MPB83 antigens was compared with the tuberculin skin test (TST), and seroprevalence was measured with this assay using serum samples collected from domesticated elk herds in Korea. The respective sensitivities of the MPB70 and MPB83 ELISAs were 51.9% (95% CI 42.0–61.6) and 49.1% (95% CI 39.3–58.9), and their specificities were 100.0% (95% CI 92.6–100.0) and 97.9% (95% CI 88.9–100.0), respectively, in comparison with the TST. The herd prevalence ranged from 50 to 80% and the mean herd seropositive rate was 67.7% (21 of 31). Of 819 serum samples, 163 (19.9%) were seropositive, and the within-region prevalence ranged from 18.5–58.0%. In conclusion, the ELISA using the MPB70 and MPB83 antigens showed moderate sensitivity and high specificity compared to TST in elk, and tuberculosis was assumed to be fairly prevalent in domesticated elk in Korea.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Establishment of ELISA using the MPB70 and MPB83 antigens for tuberculosis of elk </LI> <LI> Performance of these ELISAs (sensitivity and specificity) compared with TST </LI> <LI> Estimation of seroprevalence of tuberculosis in elk in Korea using these ELISAs </LI> <LI> First report on prevalence data of tuberculosis in domesticated elk in Korea </LI> <LI> A basic epidemiological data of tuberculosis in elk in an Asian region </LI> </UL> </P>
Salmonella typhimurium 내의 plasmid pKM101 존재와 유전자 uvrB 결손이 화학물질의 돌연변이 유발성과 독성에 미치는 영향
이세영,변우현 한국유전학회 1979 Genes & Genomics Vol.1 No.1
The effects of uvrB deletion and plasmid pKM101 on mutagenesis and cell death of Salmonella typhimurium were studied using 10 potent known chemical mutagens (MNNG, MMS, EMS, DES, Sodium azide, 4-NQO, AF-2, Niridazole, MT-C, and Ad-m). MNNG-, EMS-, and DES-induced mutagenesis were not affected in the presence of pKM101 gene product. MMS-induced mutation, however, was plasmid pKM101 dependent. R- strains were not mutagenized by the MMS treatment. Generally, alkylating agent-induced cell death was not affected by uvrB gene product. 4-NQO and AF-2 induced UV-mimic DNA damage. The effects of pKM101 and uvrB deletion on 4-NQO-induced and AF-2-induced mutagenesis and toxicity were similar to those of UV-induced mutagenesis and toxicity. Niridazole showed both UV-mimic and highly lethal effect on uvrB^- strains while MT-C and Ad-m showed less lethal effect. Plasmid pKM101 gene product showed some protection effect on MNNG, MMS, 4-NQO, and AF-2 cytotoxicities. On the other hand, no effect was observed on the toxicity caused by EMS, DES, Niridazole, MT-C, and Ad-m. All the tested compounds except alkylating agents and MT-C enhanced mutagenesis by uvrB deletion and plasmid pKM 101. The uvrB gene produce seemed to be needed in mutagenesis by MT-C.